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A simplified assay for serum 25-hydroxycalciferol.

Abstract：

:The measurement of serum 25-hydroxycalciferol (25-OH D) has proved to be a reliable index of the vitamin D nutritional status in man providing the renal 25-hydroxycalciferol-1 alpha-hydroxylase is functional. Since it is known that 25 OHD binds to alpha-globulins for its transport to the kidney, we have developed a competitive binding assay using an alpha-globulin enriched fraction (Cohn's fraction IV) as the ligand protein. A detection limit of 0.1 ng 25-OHD/tube was achieved and the non specific binding amounted to less than 3% of the initial binding. The intra- and inter-assay variations were 8.9 and 8.4% respectively. The reference values (nmol/1 +/- 2 s.d.) obtained from samples of 40 children were 50.5 +/- 21.5 with extremes of 34.8 and 95.2. The method thus proved to be a reliable and sensitive tool to assess serum 25-OHD levels.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Delvin EE,Dussault M,Glorieux FH

doi

10.1016/s0009-9120(80)90735-3

subject

Has Abstract

pub_date

1980-06-01 00:00:00

pages

106-8

issue

3

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(80)90735-3

journal_volume

13

pub_type

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