Abstract:
OBJECTIVE:The aim of our research is to determine whether the time of blood sampling and fasting of patients have an impact on TSH values. DESIGN AND METHODS:A total of 198 participants were enrolled in this study and classified into five groups: A--the first sample collection for TSH measurement was taken between 7:00 and 8:00 a.m. at fasting and the second after 140 min without food intake; B--between 7:00 and 8:00 a.m. at fasting and the second after 140 min with food intake; C--between 7:00 and 8:00 a.m. at fasting the previous day and the second one between 7:00 and 8:00 a.m. at fasting the following day; D--between 9:00 and 10:00 a.m. at fasting the previous day and the second one between 9:00 and 10:00 a.m. at fasting the following day, and E--between 9:00 and 10:00 a.m. at fasting the previous day and the second one between 7:00 and 8:00 a.m. on the following day. Serum TSH concentration was measured by electrochemiluminescence immunoassay (ECLIA, Roche Diagnostics, Mannheim, Germany). RESULTS:TSH values (mIU/L) were in group A: 2.50 (2.20-2.81) first samples, 1.74 (1.52-1.96) second samples, p<0.001; B: 2.11 (1.52-2.72) first samples, 1.56 (1.13-1.81) second samples, p<0.001; C: 2.60 (2.28-2.91) first samples, 2.23 (1.92-2.53) second samples, p<0.001; D: 1.80 (1.48-2.11) first samples, 1.77 (1.44-2.09) second samples, p<0.597; and E: 1.32 (1.11-2.16) first samples, 1.67 (1.48-2.93) second samples, p<0.001. CONCLUSION:The time of sample collection must be standardised for the purpose of standardisation and harmonisation of TSH measurements.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Mirjanic-Azaric B,Stojakovic-Jelisavac T,Vukovic B,Stojanovic D,Vujnic M,Uletilovic Sdoi
10.1016/j.clinbiochem.2015.08.020subject
Has Abstractpub_date
2015-12-01 00:00:00pages
1347-9issue
18eissn
0009-9120issn
1873-2933pii
S0009-9120(15)00400-2journal_volume
48pub_type
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更新日期:2008-10-01 00:00:00
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