Abstract:
OBJECTIVE:To report the phenotypes and genetic basis of a novel (A)γδβ(0)-thalassemia found in Thai individuals with several forms of thalassemia. DESIGNS AND METHODS:An initial study was done in an adult Thai woman who had hypochromic microcytic red cells with unusually 100% Hb F. Extended study was carried out on her parents and another 17 unrelated individuals with elevated Hb F. Hb analysis was performed by capillary electrophoresis and DNA analysis was done using PCR. A novel diagnostic method based on multiplex PCR assays was developed. RESULTS:DNA analysis of the proband revealed the homozygosity for a novel deletion of 118.3 kb, removing the entire (A)γ, ψβ, δ-, β-globin and five olfactory receptor (OR) genes with an insertion of a 179 bp inverted DNA sequence located behind the OR52A5 gene located downstream and an insertion of 7 orphan nucleotides. Her parents were both carriers of this mutation. Further screening in suspected cases in our series unexpectedly led to identification of an additional 17 cases with this mutation in different genotypes including plain heterozygote, homozygote, compound heterozygote with Hb E, and double heterozygote with several forms of α-thalassemia. Hematological features associated with these genetic interactions are presented. CONCLUSIONS:Haplotype analysis indicated a single origin of this novel deletion-inversion-insertion (A)γδβ(0)-thalassemia in the Thai population. Differentiation of this mutation and other high Hb F determinants documented previously could be done by using a developed multiplex PCR assay.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Singha K,Fucharoen G,Hama A,Fucharoen Sdoi
10.1016/j.clinbiochem.2015.03.023subject
Has Abstractpub_date
2015-07-01 00:00:00pages
703-8issue
10-11eissn
0009-9120issn
1873-2933pii
S0009-9120(15)00123-Xjournal_volume
48pub_type
杂志文章abstract:BACKGROUND:Reference intervals have traditionally been partitioned by age based on statistical significance and physiological relevance. However, analyte concentration does not change abruptly with age, but rather dynamically. In this study, we establish biochemical marker continuous reference intervals for a Canadian ...
journal_title:Clinical biochemistry
pub_type: 临床试验,杂志文章
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abstract:OBJECTIVES:In 2004, a novel grading system for papillary non-invasive bladder cancer was introduced; low grade (LG) and high grade (HG) in lieu of the former G1, G2, G3. This change allowed for increased reproducibility as well as diminished interobserver variability in histopathological grading among individual pathol...
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abstract:BACKGROUND:While investigating estrogen response element (ERE) binding properties of human estrogen receptor-α (hERα) in breast cancer cytosols, other ERE-binding proteins (ERE-BP) were observed. DESIGN AND METHODS:Recognition properties of ERE-BP were evaluated by electrophoretic mobility shift assays (EMSA) with ERE...
journal_title:Clinical biochemistry
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doi:10.1016/j.clinbiochem.2013.07.005
更新日期:2013-11-01 00:00:00
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doi:10.1016/0009-9120(92)80004-z
更新日期:1992-10-01 00:00:00
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更新日期:1990-12-01 00:00:00
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更新日期:2012-11-01 00:00:00
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doi:10.1016/j.clinbiochem.2015.05.021
更新日期:2015-11-01 00:00:00
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doi:10.1016/s0009-9120(83)90366-1
更新日期:1983-04-01 00:00:00
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doi:10.1016/j.clinbiochem.2012.09.001
更新日期:2012-12-01 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2007-01-01 00:00:00
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doi:10.1016/j.clinbiochem.2018.08.012
更新日期:2018-11-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(90)80032-e
更新日期:1990-04-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2008.02.017
更新日期:2008-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0009-9120(81)90211-3
更新日期:1981-06-01 00:00:00
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更新日期:2010-09-01 00:00:00
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pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2014.03.005
更新日期:2014-05-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2018.04.010
更新日期:2018-06-01 00:00:00
abstract:OBJECTIVES:Δ(9)-Tetrahydrocannabinol (THC) can be measured in exhaled breath by using an aerosol particle collection device. The sampling procedure is simple, non-invasive and takes only 2-3min. In the present study we measured the amount of THC in exhaled breath of cannabis users at specific time intervals up to 3h af...
journal_title:Clinical biochemistry
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doi:10.1016/j.clinbiochem.2016.06.003
更新日期:2016-09-01 00:00:00
abstract:OBJECTIVES:Studies from both experimental animals and humans suggest that administration of exogenous DHEA may have beneficial endocrine-metabolic, immunologic and neurologic effects. Several groups have administered DHEA to humans, but to the best of our knowledge, no one at this point has published a summary of the r...
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pub_type: 杂志文章
doi:10.1016/s0009-9120(99)00021-1
更新日期:1999-07-01 00:00:00
abstract:OBJECTIVES:Presence of residual blood is a common problem in cerebrospinal fluid (CSF) diagnostics of ventriculitis. We hypothesised that neutrophil granulocytes in infected, blood-containing CSF lose CD62L expression. Therefore CD62L expression on neutrophils may present a complementary marker to distinguish between p...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.07.003
更新日期:2010-11-01 00:00:00
abstract::A condition defined as nonalcoholic fatty liver disease (NAFLD) is frequently found in humans. Deemed as a benign condition until recently, more emphasis is now put on the potential harmful evolution of the inflammatory form, that is, nonalcoholic steatohepatitis (NASH), toward end-stage liver disease. This review hig...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2004.10.014
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND:A determination of serum Transthyretin (TTR, Prealbumin) level is an objective method of assessing protein catabolic loss of severely ill patients and numerous studies have shown that TTR levels correlate with patient outcomes of non-critically ill patients. We evaluated whether TTR level correlates with the...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.06.016
更新日期:2008-10-01 00:00:00
abstract:OBJECTIVES:This study investigates how some of human physiological parameters, in particular sex and daytime, can contribute to the normal variation in blood gene expression. DESIGN AND METHODS:The expressions of two genes: SOD1 (Cu/Zn) and SOD2 (Mn) in white blood cells of 19 healthy individuals (10 men and 9 women) ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.08.064
更新日期:2008-11-01 00:00:00
abstract:OBJECTIVES:Analysis of serum/plasma methylmalonic acid (MMA) is important for the diagnosis and management of methylmalonic acidemia in pediatric populations. This work focuses on developing and validating a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to monitor methylmalonic acidemia using a simpl...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2016.05.010
更新日期:2016-09-01 00:00:00