Role of sex and time of blood sampling in SOD1 and SOD2 expression variability.

Abstract:

OBJECTIVES:This study investigates how some of human physiological parameters, in particular sex and daytime, can contribute to the normal variation in blood gene expression. DESIGN AND METHODS:The expressions of two genes: SOD1 (Cu/Zn) and SOD2 (Mn) in white blood cells of 19 healthy individuals (10 men and 9 women) were examined by qPCR at 09.00, 12.00, and 15.00 h over three consecutive weeks at 7-day intervals. SOD1 and SOD2 expressions differ between sexes, higher expression being exhibited in males (P<0.005). In the male group both genes had lower expression (P<0.005) with smaller variation at 09.00 h. In females, both inter- and intra-subject, variability in SOD1 expression was increased at ovulation. Intra-individually, the expressions of genes positively correlated in males, but less in females. CONCLUSIONS:SOD1 and SOD2 expressions demonstrate how sex and daytime, merit being controlled when human gene expression analyses are evaluated, particularly within the framework of clinical trials or cohort studies.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Khymenets O,Covas MI,Farré M,Langohr K,Fitó M,de la Torre R

doi

10.1016/j.clinbiochem.2008.08.064

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

1348-54

issue

16-17

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(08)00299-3

journal_volume

41

pub_type

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