当前位置： SCI文献检索 > CLINICAL BIOCHEMISTRY期刊下所有文献 > A fluorometric high performance liquid chromatographic assay for vanillylmandelic acid (VMA) developed from a commercial kit method.

A fluorometric high performance liquid chromatographic assay for vanillylmandelic acid (VMA) developed from a commercial kit method.

Abstract：

:A fluorometric procedure based on the Bio-Rad Vanillylmandelic Acid (VMA) by High Performance Liquid Chromatography (HPLC) Test was developed. Detection by fluorescence provides better sensitivity and specificity than the Pisano spectrophotometric method. Use of a C18 mini-column extraction to extract VMA prior to HPLC analysis significantly reduced the late eluting peaks. A linear range was established up to 400 mumol/L of VMA. Within-run and between-run CV's were 3.0% and 3.7%, respectively. In a comparison of this fluorometric method with the Pisano spectrophotometric method, a linear regression of y = 0.807x + 2.185 was obtained with a correlation coefficient of 0.945. In the analysis of over 50 samples, no intereferences have been found.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Huth JA,Bottjen PL,Ludvigsen CW

doi

10.1016/s0009-9120(86)80030-3

subject

Has Abstract

pub_date

1986-08-01 00:00:00

pages

221-4

issue

4

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(86)80030-3

journal_volume

19

pub_type

杂志文章

相关文献

CLINICAL BIOCHEMISTRY文献大全
  • Proportion of conjugated bilirubin in bile in relation to hepatic bilirubin UDP-glucuronyltransferase activity.

    abstract：:To elucidate the diagnostic relevance of biliary conjugated bilirubin, biliary bilirubin from normal volunteers (NV), patients with Gilbert's syndrome (GS) and Crigler-Najjar syndrome type II (C-N II), and from various rat strains was fractionated. Biliary bilirubin diglucuronide (BDG) was present at lower levels, and...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/0009-9120(90)80024-d

    authors： Adachi Y,Yamashita M,Nanno T,Yamamoto T

    更新日期：1990-04-01 00:00:00

  • A decade of HPLC-MS/MS in the routine clinical laboratory--goals for further developments.

    abstract：:During the past decade, tandem mass spectrometry hyphenated to liquid chromatography separation systems (HPLC-MS/MS) has developed to an important technology in clinical chemistry - not only for research purposes but also for routine use. At present, most important application fields are target analyses in therapeutic...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2008.02.017

    authors： Vogeser M,Seger C

    更新日期：2008-06-01 00:00:00

  • A sensitive and rapid mass spectrometric method for the simultaneous measurement of eight steroid hormones and CALIPER pediatric reference intervals.

    abstract：OBJECTIVES:To develop an accurate assay and establish the normal reference intervals for serum cortisol, corticosterone, 11-deoxycortisol, androstenedione, 21-hydroxyprogesterone, testosterone, 17-hydroxyprogesterone, and progesterone. These steroids are commonly used as biomarkers for the diagnosis and monitoring of e...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2013.01.002

    authors： Kyriakopoulou L,Yazdanpanah M,Colantonio DA,Chan MK,Daly CH,Adeli K

    更新日期：2013-05-01 00:00:00

  • Relevance of pre-analytical blood management on the emerging cardiovascular protein biomarkers TWEAK and HMGB1 and on miRNA serum and plasma profiling.

    abstract：BACKGROUND:Disease-independent sources of biomarker variability include pre-analytical, analytical and biological variance. The aim of the present study was to evaluate whether the pre-analytical phase has any impact on the emerging heart disease TWEAK and HMGB1 protein markers and miRNA biomarkers, and whether peptido...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2016.11.005

    authors： Basso D,Padoan A,Laufer T,Aneloni V,Moz S,Schroers H,Pelloso M,Saiz A,Krapp M,Fogar P,Cornoldi P,Zambon CF,Rossi E,La Malfa M,Marotti A,Brefort T,Weis TM,Katus HA,Plebani M

    更新日期：2017-03-01 00:00:00

  • Mutation-based diagnostic testing for primary hyperoxaluria type 1: survey of results.

    abstract：OBJECTIVES:To test for specific mutations in the alanine:glyoxylate aminotransferase (AGT) gene, in order to diagnose primary hyperoxaluria type 1 (PH1). DESIGN AND METHODS:Samples of liver and/or DNA from 81 patients were submitted to our laboratory for diagnostic testing for PH1. Using a panel of selected mutations,...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.clinbiochem.2008.01.018

    authors： Coulter-Mackie MB,Lian Q,Applegarth DA,Toone J,Waters PJ,Vallance H

    更新日期：2008-05-01 00:00:00

  • Homozygous bisalbuminemia or homozygous mutant albumin?

    abstract：INTRODUCTION:Bisalbuminemia is a genetic condition in which an albumin variant is found in serum in addition to normal albumin. METHODS AND MATERIALS:Serum protein electrophoresis using the Sebia HYDRASYS electrophoresis system was performed on an 84 year old male. RESULTS:Serum protein electrophoresis showed a singl...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2011.10.005

    authors： Kulkarni K,Rowe P,Christensen CL,Higgins T

    更新日期：2012-01-01 00:00:00

  • Establishment of reference intervals of 24 chemistries in apparently healthy adult Han population of Northern China.

    abstract：BACKGROUND:The availability of reference intervals is essential for physicians to interpret laboratory results. Most of our laboratory reference intervals are derived from data on the foreign population. We have studied the reference intervals of 24 common laboratory biochemical tests in an apparently healthy adult Han...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2012.06.022

    authors： Yang S,Qiao R,Li Z,Wu Y,Yao B,Wang H,Cui L,Yang Y,Zhang J

    更新日期：2012-10-01 00:00:00

  • Comparison by LC-MS and MALDI-MS of prostate-specific antigen from five commercial sources with certified reference material 613.

    abstract：OBJECTIVE:Prostate-specific antigen (PSA) samples from five commercial sources were compared with PSA from the European Commission Community Bureau of Reference, known as CRM 613, using liquid chromatography-mass spectrometry (LC-MS) and matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS). DESIGN ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2004.10.016

    authors： Satterfield MB,Welch MJ

    更新日期：2005-02-01 00:00:00

  • Spurious hyperbilirubinemia caused by naproxen.

    abstract：OBJECTIVE:To confirm that the naproxen metabolite O-desmethylnaproxen interferes with the Jendrassik and Grof method for total bilirubin and was a possible cause of spurious hyperbilirubinemia in a case of naproxen overdose. DESIGN AND METHODS:Plasma was spiked with naproxen or O-desmethylnaproxen before total and dir...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2008.09.119

    authors： Al Riyami N,Zimmerman AC,Rosenberg FM,Holmes DT

    更新日期：2009-01-01 00:00:00

  • Simultaneous determination of fluoxetine and norfluoxetine in dried blood spots using high-performance liquid chromatography-tandem mass spectrometry.

    abstract：BACKGROUND:Therapeutic drug monitoring (TDM) of the widely prescribed antidepressant fluoxetine (FLU) is recommended in certain situations, such as occurrence of toxicity, inadequate response or suspect of poor adherence. Dried blood spot (DBS) sampling is an increasingly studied alternative for TDM, particularly for o...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2017.10.002

    authors： da Silva ACC,Raasch JR,Vargas TG,Peteffi GP,Hahn RZ,Antunes MV,Perassolo MS,Linden R

    更新日期：2018-02-01 00:00:00

  • Performance of flow cytometry to screen urine for bacteria and white blood cells prior to urine culture.

    abstract：BACKGROUND:The gold standard test for the diagnosis of urinary tract infection is bacterial culture. However, urine cultures are labor intensive and costly. Furthermore, since results take 1-2 days many patients are treated presumptively prior to culture results being known. METHODS:We evaluated the Sysmex UF-1000i fo...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2013.03.005

    authors： Giesen CD,Greeno AM,Thompson KA,Patel R,Jenkins SM,Lieske JC

    更新日期：2013-06-01 00:00:00

  • Technological trends in clinical laboratory science.

    abstract：OBJECTIVE:This article will review the advancements and new developments being made in (1) advanced computers, (2) microtechnology, (3) advanced immunodiagnostics, (4) neural networks, and (5) molecular biology. The influence of these technologies and their products on clinical laboratories is also discussed. CONCLUSI...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/0009-9120(94)00075-7

    authors： Burtis CA

    更新日期：1995-06-01 00:00:00

  • The importance of low level QC for high sensitivity troponin assays.

    abstract：BACKGROUND:With the advent of the new high-sensitivity troponin assays, it is becoming critical to measure troponin accurately to low concentrations. To ensure assay performance is acceptable, appropriate QC must be run. METHODS:In addition to the routine use of commercial QC material, we prepared pools of human QC ma...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2018.05.007

    authors： Hickman PE,Koerbin G,Badrick T,Oakman C,Potter JM

    更新日期：2018-08-01 00:00:00

  • Enhanced reduction in oxidative stress and altered glutathione and thioredoxin system response to unsaturated fatty acid load in familial hypercholesterolemia.

    abstract：OBJECTIVES:Familial hypercholesterolemia (FH) is characterized by increased oxidative stress (OS) levels. In the postprandial state, lipids and lipoproteins modulate OS status through their impact on pro-oxidant and antioxidant mechanisms. The objective of this study was to evaluate in patients with FH the response to ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2014.09.006

    authors： Cortes R,Martinez-Hervas S,Ivorra C,De Marco G,Gonzalez-Albert V,Rojo-Martínez G,Saez G,Carmena R,Ascaso JF,Real JT,Chaves FJ

    更新日期：2014-12-01 00:00:00

  • Simplified method for enzymatic urine oxalate assay.

    abstract：:A procedure for the quantitative determination of oxalate in urine suitable for use in clinical laboratories is described. Oxalate is extracted from urine and subsequently assayed by measuring the amount of hydrogen peroxide produced in an oxidation reaction catalyzed by oxalate oxidase. The reproducibility of the met...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(84)90710-0

    authors： Crider QE,Curran DF

    更新日期：1984-12-01 00:00:00

  • Batch to batch imprecision in the affinity chromatography assay of glycated hemoglobin.

    abstract：:An affinity chromatographic method (AC) was compared with ion exchange chromatography (IEC) for determination of glycated hemoglobin (gHb). In vitro, increasing glucose concentration produced no change on AC-determined gHb. gHb measurement by AC was highly correlated with mean blood glucose (r = 0.96). Mean values of ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(86)80010-8

    authors： Wettre S,von Schenck H

    更新日期：1986-12-01 00:00:00

  • Association of chitotriosidase enzyme activity and genotype with the risk of nephropathy in type 2 diabetes.

    abstract：OBJECTIVE:The immune-inflammatory system has been implicated in the pathogenesis of diabetic nephropathy; however, many of the mechanisms involved remain unclear. Chitotriosidase enzyme is an active human chitinase and a major protein product of activated macrophages. Although playing an important role in innate and ac...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.11.009

    authors： Elmonem MA,Amin HS,El-Essawy RA,Mehaney DA,Nabil M,Kamel LN,Farid IM

    更新日期：2016-04-01 00:00:00

  • Isolation of drugs from blood serum by the Du Pont Prep I Automated Sample Processor and a C18 bonded phase adsorption column.

    abstract：:We describe a fast and efficient method for the isolation of drugs from blood serum by use of the DU PONT PREP I Automated Sample Processor and octadecyl bonded phase columns. Drugs are selectively adsorbed onto C18 bonded phase and eluted with methanol. The recovered residue is essentially free of sample matrix chrom...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(81)91245-5

    authors： Repique EV,Sacks HJ,Farber SJ

    更新日期：1981-08-01 00:00:00

  • Recent advances in head and neck squamous cell carcinoma--a review.

    abstract：:The current review presents the results of the most recent studies performed on different aspects of human head and neck squamous cell carcinoma, including radiosensitivity induction, efficiency improvement of monoclonal antibodies using low-intensity ultrasound, chemical compounds such as toll-like receptor (TLC) ago...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2014.05.066

    authors： Safdari Y,Khalili M,Farajnia S,Asgharzadeh M,Yazdani Y,Sadeghi M

    更新日期：2014-09-01 00:00:00

  • Unilateral nephrectomy causes an early abrupt decrease in plasma arginine and simultaneous reduction in glomerular filtration rate in living kidney donors.

    abstract：OBJECTIVES:Living donor kidney transplantation is regarded as beneficial to allograft recipients and not particularly detrimental to the donors. Recently we have documented a reduced glomerular filtration rate (GFR) in living kidney donors (LKDs). Considering kidneys as essential for l-arginine (Arg) metabolism, in the...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2013.04.031

    authors： Zunić G,Tomić A,Spasić S

    更新日期：2013-10-01 00:00:00

  • The multiple functions and mechanisms of osteopontin.

    abstract：:Osteopontin (OPN) is a highly phosphorylated glycophosphoprotein having acidic characteristics and rich in aspartic acid. OPN, a multifunctional protein, has important functions on cardiovascular diseases, cancer, diabetes and kidney stone diseases and in the process of inflammation, biomineralization, cell viability ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2018.07.003

    authors： Icer MA,Gezmen-Karadag M

    更新日期：2018-09-01 00:00:00

  • Slow beta-migrating lipoprotein: an atherogenic subclass of low density lipoproteins.

    abstract：OBJECTIVE:To clarify the possibility that midband Lp in LDL fractions might act as an atherogenic lipoprotein in their interaction with macrophages. DESIGN AND METHODS:Low density lipoproteins (LDL) isolated by zonal ultracentrifugation from midband lipoprotein-positive serum in type lib hyperlipidemics were subjected...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/0009-9120(96)00009-c

    authors： Kawano M,Shinomiya M,Kanzaki T,Morisaki N,Shirai K,Saito Y,Yoshida S

    更新日期：1996-06-01 00:00:00

  • Stability of analytes related to clinical chemistry and bone metabolism in blood specimens after delayed processing.

    abstract：OBJECTIVES:We investigated the stability of 36 analytes related to clinical chemistry in a controlled storage study. DESIGN AND METHODS:Blood was collected from 11 subjects and was maintained for 45 min, 2.5 h, 5 h, or 24 h after phlebotomy before centrifugation. RESULTS:Statistically significant changes were observe...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2009.02.010

    authors： Zwart SR,Wolf M,Rogers A,Rodgers S,Gillman PL,Hitchcox K,Ericson KL,Smith SM

    更新日期：2009-06-01 00:00:00

  • Effect of ethanol on tumor necrosis factor signaling during liver regeneration.

    abstract：OBJECTIVES:The liver has tremendous regenerative capacity but can be damaged by toxins, such as ethanol (EtOH). It has long been known that EtOH inhibits liver regeneration. Recent work demonstrates that the proinflammatory cytokine, tumor necrosis factor ( (TNF), is required for normal liver regeneration, as well as f...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(99)00057-0

    authors： Diehl AM

    更新日期：1999-10-01 00:00:00

  • Use of different chemical methods for acid phosphatase in cases of rape.

    abstract：:Generally acid phosphatase (ACP) assay is used for testing cases of alleged rape. Comparison of three different chemical methods for Acid Phosphatase (Andersch's method with p-nitrophenyl-phosphate substrate and tartrate inhibitor (A-Tart), Roy's method with thymolphthalein phosphate substrate (R-TMP), and Babson's me...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(77)92784-9

    authors： Pragay DA,Casey SJ,Gotthelf J

    更新日期：1977-10-01 00:00:00

  • Plasma advanced glycation endproduct, methylglyoxal-derived hydroimidazolone is elevated in young, complication-free patients with Type 1 diabetes.

    abstract：OBJECTIVES:Elevated advanced glycation endproducts (AGEs) are implicated in diabetic complications. Methylglyoxal-derived hydroimidazolone (MG-H) is one of the most abundant AGEs in vivo. Our objective was to develop a time-saving, specific method to measure free MG-H in plasma and determine its levels in complication-...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2008.12.016

    authors： Han Y,Randell E,Vasdev S,Gill V,Curran M,Newhook LA,Grant M,Hagerty D,Schneider C

    更新日期：2009-05-01 00:00:00

  • Cystatin C as a predictor of all-cause mortality and myocardial infarction in patients with non-ST-elevation acute coronary syndrome.

    abstract：OBJECTIVES:To investigate the predictive value of cystatin C among patients diagnosed with non-ST-elevation acute coronary syndrome (nSTE-ACS). DESIGN AND METHODS:Admission serum samples from 245 nSTE-ACS patients were measured with a novel cystatin C immunoassay based on a dry-reagent, double monoclonal design. Creat...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2012.02.012

    authors： Ristiniemi N,Lund J,Tertti R,Christensson A,Ilva T,Porela P,Pulkki K,Pettersson K

    更新日期：2012-05-01 00:00:00

  • Comparison of new solid-phase extraction methods for chromatographic identification of drugs in clinical toxicological analysis.

    abstract：OBJECTIVES:Newly presented solid-phase extraction methods (Solid Phase Disc Extraction, SPEC-Plus, Multi-Modal, and Solid Phase Micro Extraction, SPME have been checked with respect to their applicability to clinical toxicological analysis. In comparison with conventional liquid/liquid-extraction and a common mixed-pha...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(96)00096-3

    authors： Degel F

    更新日期：1996-12-01 00:00:00

  • The importance of circulating levels of salusin-α, salusin-β, and heregulin-β1 in atherosclerotic coronary arterial disease.

    abstract：OBJECTIVE:The relationship between the severity of atherosclerotic coronary artery disease (CAD) and circulating levels of salusin-α, salusin-β and heregulin-β1 has been investigated. In addition, the relationship with these peptides and high sensitive C-reactive protein (hsCRP) has been investigated. METHODS:The stud...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2020.10.003

    authors： Arkan A,Atukeren P,Ikitimur B,Simsek G,Koksal S,Gelisgen R,Ongen Z,Uzun H

    更新日期：2021-01-01 00:00:00

  • Alpha-1-antitrypsin molecular testing in Canada: A seven year, multi-centre comparison.

    abstract：BACKGROUND:Laboratory confirmation of alpha-1-antitrypsin (A1AT) deficiency may be achieved by multiple methods. Here, we compare the relative comprehensiveness and efficiency of pathogenic variant (PV) detection of four different protocols utilized at different diagnostic centres in Canada. METHODS:Diagnostic results...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,多中心研究

    doi：10.1016/j.clinbiochem.2020.05.001

    authors： Mattman A,Gilfix BM,Chen SX,DeMarco ML,Kyle BD,Parker ML,Agbor TA,Jung B,Selvarajah S,Barakauskas VE,Vaags AK,Estey MP,Nelson TN,Speevak MD

    更新日期：2020-07-01 00:00:00