Batch to batch imprecision in the affinity chromatography assay of glycated hemoglobin.

Abstract:

:An affinity chromatographic method (AC) was compared with ion exchange chromatography (IEC) for determination of glycated hemoglobin (gHb). In vitro, increasing glucose concentration produced no change on AC-determined gHb. gHb measurement by AC was highly correlated with mean blood glucose (r = 0.96). Mean values of gHb were apparently higher, and variation greater, by AC than by IEC. Intra-assay variation was 2.0% and 3.0%, respectively. Inter-assay variation was 4.8% by IEC and 6.4% by AC. When different batches of AC-columns were compared, mean values as well as precision differed considerably. The affinity chromatographic method tested here shows several expected advantages, but precision and inter-batch variation of the columns remain to be improved.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Wettre S,von Schenck H

doi

10.1016/s0009-9120(86)80010-8

subject

Has Abstract

pub_date

1986-12-01 00:00:00

pages

364-6

issue

6

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(86)80010-8

journal_volume

19

pub_type

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