当前位置： SCI文献检索 > CLINICAL BIOCHEMISTRY期刊下所有文献 > Isolation of drugs from blood serum by the Du Pont Prep I Automated Sample Processor and a C18 bonded phase adsorption column.

Isolation of drugs from blood serum by the Du Pont Prep I Automated Sample Processor and a C18 bonded phase adsorption column.

Abstract：

:We describe a fast and efficient method for the isolation of drugs from blood serum by use of the DU PONT PREP I Automated Sample Processor and octadecyl bonded phase columns. Drugs are selectively adsorbed onto C18 bonded phase and eluted with methanol. The recovered residue is essentially free of sample matrix chromogens and shows very low background on thin layer chromatography. It is also suitable for analysis by gas liquid chromatography and high pressure liquid chromatography. Recovery of drugs added to serum is greater than 90 per cent for most drugs studied which include weakly acidic, neutral and alkaline drugs. The method offers tremendous savings to the user in time and dollars, since it is fully automated from the extraction step to the drying of the extract, and the columns are reusable at least 10 times. Up to 12 samples can be processed simultaneously in less than 60 minutes.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Repique EV,Sacks HJ,Farber SJ

doi

10.1016/s0009-9120(81)91245-5

subject

Has Abstract

pub_date

1981-08-01 00:00:00

pages

196-200

issue

4

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(81)91245-5

journal_volume

14

pub_type

杂志文章

相关文献

CLINICAL BIOCHEMISTRY文献大全
  • Plasma TNF-R1 and insulin concentrations in relation to leptin levels among normal and overweight children.

    abstract：OBJECTIVES:Leptin is a multifunctional polypeptide produced primarily by adipocytes and associated with the occurrence of obesity and insulin resistance. Tumor necrosis factor-alpha (TNF-alpha), a polypeptide cytokine produced primarily by mononuclear phagocytes, plays a key role in the initiation of the inflammatory r...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(02)00314-4

    authors： Chu NF,Shen MH,Wu DM,Shieh SM

    更新日期：2002-06-01 00:00:00

  • Association of glutathione-S-transferase gene polymorphism and lipoprotein subclasses in hemodialysis patients.

    abstract：OBJECTIVES:End-stage renal disease (ESRD) is characterized by profound dyslipidemia and enhanced oxidative stress. The patients also show evidence of exhausted and/or deficient anti-oxidative defense enzymes, one of them being glutathione-S-transferase (GST). This study investigates relationship between GST gene polymo...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2013.11.011

    authors： Vekic J,Zeljkovic A,Jelic-Ivanovic Z,Damjanovic T,Suvakov S,Matic M,Savic-Radojevic A,Simic T,Spasojevic-Kalimanovska V,Gojkovic T,Spasic S,Dimkovic N

    更新日期：2014-04-01 00:00:00

  • Plasma arginase concentration measured by an enzyme-linked immunosorbent assay (ELISA) in normal adult population.

    abstract：:Human liver arginase has many biological effects on lymphocytes, macrophages, liver cells, and tumor cells, in addition to its major role in the liver urea cycle. We have developed a sandwich enzyme-linked immunosorbent assay (ELISA) method to quantitate arginase concentrations in plasma that can be applied to various...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/0009-9120(93)80009-j

    authors： Wang SR,Chen ML,Huang MH,Lin HY,Tsai JJ,Kuo BI

    更新日期：1993-12-01 00:00:00

  • Triglyceride assay using the ABA-50 and Boehringer Mannheim Triglyceride reagent kit.

    abstract：:1. A procedure for the assay of triglycerides on the ABA-50 is described. 2. The Boehringer Mannheim Triglyceride (Fully Enzymatic method) reagent kit has been evaluated with this method and was found to be satisfactory. ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(77)93190-3

    authors： Owen VM,Tai OH

    更新日期：1977-12-01 00:00:00

  • Ornithine methyl ester. An unusual metabolite encountered in the urine of patients with a urea cycle disorder characterized by hyperammonemia, hyperornithinemia and homocitrullinuria.

    abstract：:In the urine of six subjects with a urea cycle disorder characterized by hyperammonemia, hyperornithinemia and homocitrullinuria, an unusual ninhydrin-reaction compound was encountered. This unknown on hydrolysis yielded ornithine as the only amino acid and, on dansylation studied, yielded didansyl ornithine. The meta...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(77)90986-9

    authors： Gordon BA,Gatfield PD,Taller E

    更新日期：1977-04-01 00:00:00

  • Variations in ovarian steroid levels during the luteal phase of the menstrual cycle.

    abstract：:1. Six subjects were investigated during the luteal phase of the menstrual cycle to determine whether the ovarian steroids, estrogen and progesterone were released in an episodic manner. 2. Analysis of plasma obtained every 20 min. during a normal working day provided no evidence for pulsatile release of either steroi...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(75)92479-0

    authors： Younglai EV,Smith SL,Cleghorn JM,Streiner DL

    更新日期：1975-08-01 00:00:00

  • Urinary kidney injury molecule-1 and neutrophil gelatinase-associated lipocalin as indicators of tubular damage in normoalbuminuric patients with type 2 diabetes.

    abstract：OBJECTIVES:Renal dysfunction has been reported in normoalbuminuric patients, demonstrating the necessity to improve the diagnostic and prognostic tools for diabetic kidney disease (DKD) investigation. Therefore, the aim of this study was to investigate whether the urinary levels of neutrophil gelatinase-associated lipo...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.10.016

    authors： de Carvalho JA,Tatsch E,Hausen BS,Bollick YS,Moretto MB,Duarte T,Duarte MM,Londero SW,Premaor MO,Comim FV,Delanghe JR,Moresco RN

    更新日期：2016-02-01 00:00:00

  • Serum testosterone quantitation by liquid chromatography-tandem mass spectrometry: interference from blood collection tubes.

    abstract：OBJECTIVES:During the development of a testosterone assay by LC-MS/MS, we encountered significant assay interference introduced by blood collection tubes. We examined a number of commonly used blood collection tubes for the presence of interference and its impact on testosterone quantitation. DESIGN AND METHODS:A numb...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2012.08.008

    authors： Shi RZ,van Rossum HH,Bowen RA

    更新日期：2012-12-01 00:00:00

  • The effect of the mode of delivery on the maternal-neonatal erythrocyte membrane acetylcholinesterase activity.

    abstract：UNLABELLED:Free radical production and high catecholamine levels are implicated with the modulation of acetylcholinesterase (AChE) activity. OBJECTIVE:To investigate the effect of the mode of delivery on maternal-neonatal erythrocyte membrane AChE activity. SUBJECTS AND METHODS:Some women with normal pregnancy (N = 3...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2008.04.005

    authors： Vlachos DG,Schulpis KH,Parthimos T,Mesogitis S,Vlachos GD,Partsinevelos GA,Antsaklis A,Tsakiris S

    更新日期：2008-07-01 00:00:00

  • Molecular and hematological characteristics of a novel form of α-globin gene triplication: the hemoglobin St.Luke's-Thailand [α95(G2)Pro→Arg] or Hb St. Luke's [A2] HBA2.

    abstract：OBJECTIVES:This study aims to report a hitherto un-described α-globin gene triplicated allele with a novel hemoglobin (Hb) variant on α2-globin gene, Hb St.Luke's-Thailand [α95(G2)Pro-Arg]. DESIGNS AND METHODS:A study was done on a 1.5-year-old Thai boy who showed pallor and jaundice after birth. Hb analysis was perfo...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2013.01.022

    authors： Singha K,Fucharoen G,Jetsrisuparb A,Fucharoen S

    更新日期：2013-05-01 00:00:00

  • Elevation of carbohydrate antigen 125 in chronic heart failure may be caused by mechanical extension of mesothelial cells from serous cavity effusion.

    abstract：OBJECTIVES:The practical application of elevated carbohydrate antigen 125 (CA125) to predict clinical outcome in chronic heart failure (CHF) is under debate. The mechanism for this CA125 elevation remains unknown. We hypothesize that mechanical stress on mesothelial cells initiates CA125 synthesis. DESIGN AND METHODS:...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2013.09.008

    authors： Huang F,Zhang K,Chen J,Cai Q,Liu X,Wang T,Lv Z,Wang J,Huang H

    更新日期：2013-11-01 00:00:00

  • Activity and protein level of CuZnSOD and MnSOD in benign and malignant liver tumors.

    abstract：OBJECTIVES:Superoxide dismutase (SOD) is a key antioxidant enzyme, responsible for scavenging of superoxide anion - a precursor of all reactive oxygen species (ROS). ROS are implicated in many pathologies, particularly in tumor disease. The aim of our work was to evaluate SOD isoforms' activity and protein level change...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2007.09.014

    authors： Skrzycki M,Scibior D,Podsiad M,Czeczot H

    更新日期：2008-01-01 00:00:00

  • The use of proteomics for the assessment of clinical samples in research.

    abstract：:Proteomics, the analysis of expressed proteins, has been an important developing area of research for the past two decades [Anderson, NG, Anderson, NL. Twenty years of two-dimensional electrophoresis: past, present and future. Electrophoresis 1996;17:443-453]. Advances in technology have led to a rapid increase in app...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,评审

    doi：10.1016/j.clinbiochem.2004.09.002

    authors： Aldred S,Grant MM,Griffiths HR

    更新日期：2004-11-01 00:00:00

  • Telomerase activity, and tissue polypeptide specific antigen (TPS) in Egyptian breast cancer patients.

    abstract：OBJECTIVES:Breast cancer is the most common malignancy among Egyptian women. The aim of this study is to evaluate the role of both telomerase and TPS estimation in assessment of breast cancer. METHODS:The study included 40 patients with breast cancer, and 20 patients with benign breast diseases. Telomerase activity in...

    journal_title：Clinical biochemistry

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0009-9120(01)00253-3

    authors： Kassim SK,Fawzy H,El Rassad MM,Khalil FK,Khalifa A

    更新日期：2001-09-01 00:00:00

  • Beta-hexosaminidase isoenzyme profiles in serum, plasma, platelets and mononuclear, polymorphonuclear and unfractionated total leukocytes.

    abstract：OBJECTIVES:The relative proportion in percentage of the isoenzyme A of beta-hexosaminidase (Hex) is the single discriminatory function most frequently used for the biochemical screening of heterozygote Tay-Sachs disease carriers. It has been suggested that the assay of the Hex isoenzymes in homogeneous cell preparation...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2005.05.016

    authors： Casal JA,Cano E,Tutor JC

    更新日期：2005-10-01 00:00:00

  • Establishment of reference intervals for the salivary cortisol circadian cycle, by electrochemiluminescence (ECLIA), in healthy adults.

    abstract：OBJECTIVES:To determine salivary cortisol reference intervals in a healthy adult population, at 6 different time points during a 24-hour (h) period. METHODS:In a prospective study, salivary cortisol concentrations were measured upon waking, one-hour post-waking and at specific times of the day: at 12 h00, 16 h00, 20 h...

    journal_title：Clinical biochemistry

    pub_type： 临床试验,杂志文章

    doi：10.1016/j.clinbiochem.2018.02.012

    authors： Gagnon N,Fréchette I,Mallet PL,Dubé J,Houde G,Fink GD

    更新日期：2018-04-01 00:00:00

  • Association between oxidative stress and changes of trace elements in patients with breast cancer.

    abstract：OBJECTIVES:To investigate the association between oxidative stress and certain trace elements in the blood of breast cancer patients. DESIGN AND METHODS:Malondialdehyde (MDA) was measured in serum of patients with breast cancer (n = 35) and controls (n = 35) by high performance liquid chromatography. Trace elements we...

    journal_title：Clinical biochemistry

    pub_type： 临床试验,杂志文章

    doi：10.1016/s0009-9120(98)00096-4

    authors： Huang YL,Sheu JY,Lin TH

    更新日期：1999-03-01 00:00:00

  • Serum HDL-C levels, log (TG/HDL-C) values and serum total cholesterol/HDL-C ratios significantly correlate with radiological extent of disease in patients with community-acquired pneumonia.

    abstract：BACKGROUND:In several studies, it was shown that there was a marked decrease in serum levels of HDL-C during infection and inflammation in general. In particular, a decrease in the level of serum HDL-C was also shown in pneumonia. Correlations between inflammatory markers such as acute phase proteins, cytokines and ser...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2006.01.015

    authors： Deniz O,Tozkoparan E,Yaman H,Cakir E,Gumus S,Ozcan O,Bozlar U,Bilgi C,Bilgic H,Ekiz K

    更新日期：2006-03-01 00:00:00

  • Efficacy of simultaneous determination of bone alkaline phosphatase mass concentration in serum and urinary excretion of pyridinium cross-links for detection of bone metastases.

    abstract：OBJECTIVES:To examine the diagnostic validity of bone alkaline phosphatase (BAP) in serum (a marker of bone formation) and of urinary excretion of pyridinium cross-links (PYR) (a marker of bone resorption) for detection of bone metastases. DESIGN AND METHODS:Two heterogeneous immunoassays were employed for determinati...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/0009-9120(95)96621-a

    authors： Withold W,Georgescu G,Khakzad H,Vosberg H,Mueller-Gaertner HW,Reinauer H

    更新日期：1995-10-01 00:00:00

  • Macro creatine kinase type 2 in human colonic tissues.

    abstract：:Macro creatine kinase type 2 (MCK-2) is a promising new tumour marker for carcinoma of the colon. We have purified it from tumour tissue, from serum and, for the first time, from normal colonic epithelium and identified it in a tissue culture line of colon cancer cells. The molecular weight of each preparation, regard...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(85)80071-0

    authors： Rogalsky VY,Koven IH,Miller DR,Pollard A

    更新日期：1985-12-01 00:00:00

  • Matrix metalloproteinase7 -181A/G polymorphism is associated with increased cancer risk among high-quality studies: Evidence from a meta-analysis.

    abstract：OBJECTIVES:To evaluate the effect of Matrix metalloproteinase 7 -181A/G polymorphism (rs11568818) on the risk of cancer, we performed a meta-analysis. DESIGN AND METHODS:24 case-control studies were identified by searching Pubmed, Embase, ISI, OVID MEDLINE and EBSCO databases. Odd ratios and corresponding 95% confiden...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章,meta分析

    doi：10.1016/j.clinbiochem.2013.07.015

    authors： Wu J,Guan X,Li YT,Bai P,Wu J

    更新日期：2013-11-01 00:00:00

  • Performance characteristics of beta-2-microglobulin measurements on a Beckman Immage analyzer with the DakoCytomation reagent kit.

    abstract：OBJECTIVE:Evaluation of serum beta-2-microglobulin (beta2M) in an automated analyzer. DESIGN AND METHODS:The DakoCytomation beta2M kit is an antibody based reagent intended for quantitative determination of beta2M in serum and plasma by rate nephelometry. RESULTS:The limit of blank is 0.16 mg/L. The method is linear ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2009.06.030

    authors： De BK,Jimenez E,De S

    更新日期：2009-10-01 00:00:00

  • Regulation of apolipoprotein B biogenesis in human hepatocytes: posttranscriptional control mechanisms that determine the hepatic production of apolipoprotein B-containing lipoproteins.

    abstract：OBJECTIVES:Hepatic overproduction of apolipoprotein B (apoB)-containing lipoproteins appears to be a common cause of hyperlipoproteinemia in humans. Patients with overproduction states secrete denser cholesterol ester-rich lipoprotein particles which are highly atherogenic. The formation of apoB particles involves a ve...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/0009-9120(95)00007-v

    authors： Adeli K,Mohammadi A,Macri J

    更新日期：1995-04-01 00:00:00

  • The effect of specimen collection additives and sample storage on the Dupont ACA glucose method.

    abstract：:Two studies are described in this paper for the purpose of extending the application of the Dupont ACA glucose method to (1) analysis of specimens containing sodium fluoride and sodium iodoacetate, and (2) analysis of specimens containing EDTA, provided analysis is conducted immediately following specimen collection a...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(82)96520-1

    authors： Dubois JA,Roberts G,Beekman J

    更新日期：1982-04-01 00:00:00

  • Effects of metamizol and magnesium sulfate on enzyme activity of glucose 6-phosphate dehydrogenase from human erythrocyte in vitro and rat erythrocyte in vivo.

    abstract：OBJECTIVE:Effects of metamizol and magnesium sulfate on erythrocyte glucose 6-phosphate dehydrogenase enzyme activity were investigated in in vitro and in vivo conditions. METHODS:For in vitro studies, glucose 6-phosphate dehydrogenase was purified from human erythrocyte and rats were used for in vivo studies. Enzyme ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(01)00207-7

    authors： Ciftçi M,Ozmen I,Büyükokuroğlu ME,Pençe S,Küfrevioğlu OI

    更新日期：2001-06-01 00:00:00

  • The effect of nephropathy on plasma sphingosine 1-phosphate concentrations in patients with type 2 diabetes.

    abstract：OBJECTIVES:Sphingosine 1-phosphate (S1P) is carried in plasma by the HDL particles and albumin. It mediates several protective functions of HDL. Because of its barrier-enhancing effect, it has attracted attention in diseases associated with endothelial dysfunction. We examined the impact of circulating levels of S1P in...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2015.08.001

    authors： Bekpinar S,Yenidunya G,Gurdol F,Unlucerci Y,Aycan-Ustyol E,Dinccag N

    更新日期：2015-12-01 00:00:00

  • Mitochondrial dysfunction in skin biopsies and blood mononuclear cells from two cases of fibromyalgia patients.

    abstract：OBJECTIVES:Coenzyme Q(10) (CoQ(10)) is an essential electron carrier in the mitochondrial respiratory chain and a strong antioxidant. Signs associated with skin alteration and mitochondrial dysfunction have been observed in patients with fibromyalgia (FM). The aim of this study was to analyze CoQ(10) levels, mitochondr...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2010.06.013

    authors： Cordero MD,Moreno-Fernández AM,Carmona-López MI,Sánchez-Alcázar JA,Rodríguez AF,Navas P,de Miguel M

    更新日期：2010-09-01 00:00:00

  • A sensitive method for the determination of hydrochlorothiazide in serum by high pressure liquid chromatography.

    abstract：:A high pressure liquid chromatographic method for the determination of hydrochlorothiazide in serum has been elaborated and verified in a blood level study in normal individuals given a single oral dose of 50 mg hydrochlorothiazide. Based on 5 ml of serum, the limit of detection of the method is 2 ng/ml. ...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/s0009-9120(78)90434-4

    authors： Robinson WT,Cosyns L

    更新日期：1978-08-01 00:00:00

  • Plasma circular RNA panel acts as a novel diagnostic biomarker for colorectal cancer.

    abstract：BACKGROUND:Colorectal cancer (CRC) is one of the most common cancers worldwide, and emerging lines of evidence have implicated circular RNAs (circRNAs), a novel class of endogenous noncoding RNAs, in CRC development. However, whether plasma circRNAs might be novel diagnostic biomarkers for CRC remains unclear. METHODS...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2019.10.012

    authors： Lin J,Cai D,Li W,Yu T,Mao H,Jiang S,Xiao B

    更新日期：2019-12-01 00:00:00

  • Quality assessment of microarray experiments.

    abstract：OBJECTIVES:We describe a quality assurance procedure to maximize the value of oligonucleotide microarray expression profiles. DESIGN, METHODS, AND RESULTS:Background microarray noise was 82.2+/-54.5 and 51.8+/-12.4 units, respectively, before and after enacting the program (P<0.0001). We also noted improved concordanc...

    journal_title：Clinical biochemistry

    pub_type： 杂志文章

    doi：10.1016/j.clinbiochem.2005.04.010

    authors： Carter DE,Robinson JF,Allister EM,Huff MW,Hegele RA

    更新日期：2005-07-01 00:00:00