Ornithine methyl ester. An unusual metabolite encountered in the urine of patients with a urea cycle disorder characterized by hyperammonemia, hyperornithinemia and homocitrullinuria.

abstract：BACKGROUND:Histamine (HA) is a small amine playing an important role in anaphylactic reactions. In order to identify and quantify HA in plasma matrix, different methods have been developed but present several disadvantages. Here, we developed an alternative method using liquid chromatography coupled with an ultra-high ...

journal_title：Clinical biochemistry

authors： Laurichesse M,Gicquel T,Moreau C,Tribut O,Tarte K,Morel I,Bendavid C,Amé-Thomas P

更新日期：2016-01-01 00:00:00

abstract：OBJECTIVES:Cortisol metabolism is controlled by 11beta-hydroxysteroid dehydrogenase (11beta-HSD) isoenzymes, which interconvert cortisol and cortisone. Accurate measurement of the cortisol and cortisone concentrations and their ratio provide useful information about 11beta-HSD activity. METHODS:Cortisol and cortisone ...

journal_title：Clinical biochemistry

authors： Kushnir MM,Neilson R,Roberts WL,Rockwood AL

更新日期：2004-05-01 00:00:00

abstract：OBJECTIVES:Behçet's disease is a multisystem disorder characterized by a chronic inflammation including acute attacks and remission periods. Decreased enzyme activity of the antioxidant system and increased levels of free radicals may have important roles in the damage of tissues observed in the disease period. In addi...

journal_title：Clinical biochemistry

authors： Orem A,Yandi YE,Vanizor B,Cimşit G,Uydu HA,Malkoç M

更新日期：2002-05-01 00:00:00

abstract：OBJECTIVE:Salivary cortisol in the assessment of glucocorticoid related disorders. DESIGN-METHODS: Serum and salivary cortisol were measured in 189 patients (22 Cushing's syndrome, 67 pseudo-Cushing, 11 Addison's disease, 89 controls) at 8:00 and 24:00 h. RESULTS:Serum and salivary cortisol correlated in the whole stu...

journal_title：Clinical biochemistry

authors： Restituto P,Galofré JC,Gil MJ,Mugueta C,Santos S,Monreal JI,Varo N

更新日期：2008-06-01 00:00:00

abstract：:Generally acid phosphatase (ACP) assay is used for testing cases of alleged rape. Comparison of three different chemical methods for Acid Phosphatase (Andersch's method with p-nitrophenyl-phosphate substrate and tartrate inhibitor (A-Tart), Roy's method with thymolphthalein phosphate substrate (R-TMP), and Babson's me...

journal_title：Clinical biochemistry

authors： Pragay DA,Casey SJ,Gotthelf J

更新日期：1977-10-01 00:00:00

abstract：:A fluorometric procedure based on the Bio-Rad Vanillylmandelic Acid (VMA) by High Performance Liquid Chromatography (HPLC) Test was developed. Detection by fluorescence provides better sensitivity and specificity than the Pisano spectrophotometric method. Use of a C18 mini-column extraction to extract VMA prior to HPL...

journal_title：Clinical biochemistry

authors： Huth JA,Bottjen PL,Ludvigsen CW

更新日期：1986-08-01 00:00:00

abstract：OBJECTIVES:To compare HPLC methods with short and long elution times for HbA1c measurement in blood. METHODS:Comparison between G7-Tosoh (1.2 min); Bio-Rad-Variant-II-Turbo (1.3 min) and Arkray-HA-8160 (2.9 min). RESULTS:Passing-Bablok regression equations were: Y=0.17+0.96X; Y=-0.39+1.01X; Y=-0.40+1.0X and the means...

journal_title：Clinical biochemistry

authors： Leon-Justel A,Santotoribio JD,Dominguez-Pascual I,Delgado AL,Macias C,Herrera MT,Leal A,Mangas MA,Acosta D,Guerrero JM

更新日期：2009-10-01 00:00:00

abstract：:During sequential physiologic maturation of an individual follicle, the number of granulosa cells increases in excess of 1000-fold, while intra-ovarian concentrations of sex steroids escalate by 100-fold. The recent development of several in vitro ovarian systems has permitted a more extensive and direct assessment of...

journal_title：Clinical biochemistry

authors： Veldhuis JD

更新日期：1981-10-01 00:00:00

abstract：OBJECTIVES:There has been growing interest in the use of serum B-type natriuretic peptide (BNP) and the N-terminal segment of its pro-hormone (NT-proBNP) as biomarkers for cardiac disease. The aim of this review is to summarize the current state of knowledge regarding BNP and NT-proBNP measurement in the pediatric popu...

journal_title：Clinical biochemistry

authors： Davis GK,Bamforth F,Sarpal A,Dicke F,Rabi Y,Lyon ME

更新日期：2006-06-01 00:00:00

abstract：:A procedure for the quantitative determination of oxalate in urine suitable for use in clinical laboratories is described. Oxalate is extracted from urine and subsequently assayed by measuring the amount of hydrogen peroxide produced in an oxidation reaction catalyzed by oxalate oxidase. The reproducibility of the met...

journal_title：Clinical biochemistry

authors： Crider QE,Curran DF

更新日期：1984-12-01 00:00:00

abstract：:Three commercially available assays (an enzyme-linked immunosorbent assay ELISA, an immunoradiometric assay, IRMA, and a nephelometric assay) for the determination of lipoprotein(a) [Lp(a)] were compared with respect to the dependency of these assays on the various apolipoprotein(a) [apo(a)] isoforms. Although there w...

journal_title：Clinical biochemistry

authors： Leus FR,Leerink CB,Prins J,van Rijn HJ

更新日期：1994-12-01 00:00:00

abstract：:Many clinical laboratories use the Beckman Microzone electrophoretic system (Beckman Instruments Inc. Fullerton, CA 92634) for serum protein separation. Although detailed instruction is supplied in the manufacturer's manual (1) for protein separation, the effect of different sample application positions on the cellulo...

journal_title：Clinical biochemistry

authors： Chu SY,Turkington VE

更新日期：1978-12-01 00:00:00

abstract：OBJECTIVE:A survey of operators of a bedside blood glucose monitoring (BGM) program at a tertiary health care institution was performed to identify potential outcome indicators for our quality assurance program. DESIGN AND METHODS:170 surveys were randomly distributed to each nursing unit. The survey consisted of 20 q...

journal_title：Clinical biochemistry

authors： Collier CP,Houlden RL,Gleeson KA,Patrzykat A,Rhymer SL

更新日期：1998-06-01 00:00:00

abstract：:Immunoturbidimetric assays for specific proteins are available on "open system" clinical chemistry analyzers. The analytical performance of nine immunoturbidimetric specific protein assays (C3, C4, CRP, Haptoglobin, IgA, IgG, IgM, RF, and Transferrin) was compared to immunonephelometry. Testing was performed on the Ab...

journal_title：Clinical biochemistry

authors： Mali B,Armbruster D,Serediak E,Ottenbreit T

更新日期：2009-10-01 00:00:00

abstract：OBJECTIVE:To investigate the feasibility of detecting circulating cancer cells in peripheral blood from gastric cancer patients using Piwi-interacting RNAs (piRNAs) as markers. DESIGN AND METHODS:Real-time quantitative reverse transcription-polymerase chain reaction was used to analyze piR-651 and piR-823 levels in th...

journal_title：Clinical biochemistry

authors： Cui L,Lou Y,Zhang X,Zhou H,Deng H,Song H,Yu X,Xiao B,Wang W,Guo J

更新日期：2011-09-01 00:00:00

abstract：OBJECTIVE:Dyslipidemia is commonly seen in patients with end-stage renal disease (ESRD). This prospective study investigates whether small-sized high-density lipoprotein (HDL) particles alone or in combination with high sensitivity C-reactive protein (hsCRP) are independent determinants of ESRD mortality. DESIGN AND M...

journal_title：Clinical biochemistry

authors： Vekic J,Zeljkovic A,Bogavac-Stanojevic N,Jelic-Ivanovic Z,Spasojevic-Kalimanovska V,Simic-Ogrizovic S,Dopsaj V,Spasic S

更新日期：2011-06-01 00:00:00

abstract：OBJECTIVES:Recurrent miscarriage (RM) is a challenging medical problem because of its unknown pathogenesis and etiology in most of the cases. Recent studies suggest the role of persistent environmental pollutants such as organochlorine pesticides (OCPs) in the etiology of RM. The present study was conducted to investig...

journal_title：Clinical biochemistry

authors： Pathak R,Mustafa M,Ahmed RS,Tripathi AK,Guleria K,Banerjee BD

更新日期：2010-01-01 00:00:00

abstract：OBJECTIVES:Increased free radical activity and lipid peroxidation may be implicated in the pathogenesis of preeclampsia. This study was initiated to assess antioxidant enzyme and trace metals's status in preeclampsia. DESIGN AND METHODS:The comparison was made between the pregnant women with or without preeclampsia an...

journal_title：Clinical biochemistry

authors： Ilhan N,Ilhan N,Simsek M

更新日期：2002-07-01 00:00:00

abstract：OBJECTIVE:The aim of our research is to determine whether the time of blood sampling and fasting of patients have an impact on TSH values. DESIGN AND METHODS:A total of 198 participants were enrolled in this study and classified into five groups: A--the first sample collection for TSH measurement was taken between 7:0...

journal_title：Clinical biochemistry

authors： Mirjanic-Azaric B,Stojakovic-Jelisavac T,Vukovic B,Stojanovic D,Vujnic M,Uletilovic S

更新日期：2015-12-01 00:00:00

abstract：OBJECTIVES:We propose a simple way to reliably rank assays for improvement according to patient risk, based solely on EQA imprecision and biological variation data. Because the underlying technique aligns the imprecision class of an assay from EQA data, peer performance can be used to assess achievable imprecision and ...

journal_title：Clinical biochemistry

authors： Mackay M,Hegedus G,Badrick T

更新日期：2016-05-01 00:00:00

abstract：:Liquid chromatography (LC) coupled to mass spectrometry (MS) or tandem mass spectrometry (MS/MS) has become increasingly important in clinical and forensic toxicology as well as doping control and is now a robust and reliable technique for routine analysis in these fields. In recent years, methods for LC-MS(/MS)-based...

journal_title：Clinical biochemistry

authors： Peters FT

更新日期：2011-01-01 00:00:00

abstract：BACKGROUND:Vitamin D-binding protein (DBP) is the main systemic transporter of 1.25(OH)2D3 and is essential for its cellular endocytosis. There are two known polymorphisms in exon 11 of the DBP gene resulting in amino acid variants: GAT-->GAG substitution replaces aspartic acid by glutamic acid in codon 416; and ACG-->...

journal_title：Clinical biochemistry

authors： Ongagna JC,Pinget M,Belcourt A

更新日期：2005-05-01 00:00:00

abstract：:We evaluated a method for quantifying bone isoenzyme of alkaline phosphatase (ALP) which utilizes wheat-germ lectin to precipitate this fraction. In precision studies, CVs ranged from 3.2 to 11.4% (within-day) and from 3.7 to 11.5% (day-to-day). The assay procedure was linear to 1100 U/L and was easily adapted to auto...

journal_title：Clinical biochemistry

authors： Burlina A,Plebani M,Secchiero S,Zaninotto M,Sciacovelli L

更新日期：1991-10-01 00:00:00

abstract：OBJECTIVES:To investigate the possible association between functional variant Ala-9Val in the MnSOD gene and asthma in the case-control study comprising 626 Caucasian subjects. METHODS:MnSOD genotypes were determined by PCR with subsequent restriction analysis by the BsaWI enzyme. RESULTS:Significant differences in a...

journal_title：Clinical biochemistry

authors： Holla LI,Kankova K,Vasku A

更新日期：2006-03-01 00:00:00

abstract：OBJECTIVES:We established population-based reference limits for plasma total homocysteine (tHcy) according to creatinine. DESIGN AND METHODS:In 7042 middle-aged and elderly subjects from the Hordaland Homocysteine Study, we used statistical modeling to establish nomograms for tHcy according to creatinine in the whole ...

journal_title：Clinical biochemistry

authors： Elshorbagy AK,Oulhaj A,Konstantinova S,Nurk E,Ueland PM,Tell GS,Nygård O,Vollset SE,Refsum H

更新日期：2007-11-01 00:00:00

abstract：OBJECTIVE:We performed a side-by-side comparison between three stain-then-lyse commercially available methods (Ortho-mune Lysing solution, FACS lysing solution and ImmunoPrep reagent system) and a lyse-then-stain method using hypotonic NH4Cl. The major difference between these methods is that only in the latter the ali...

journal_title：Clinical biochemistry

authors： Pacifici R,Zuccaro P,Cozzi-Lepre A,di Carlo S,Bacosi A,Fattorossi A

更新日期：1998-04-01 00:00:00

abstract：OBJECTIVES:The aim of the study was to evaluate the concentration of interleukin 12 (IL-12), the activity of phospholipase A(2) (PLA(2)), and platelet-activating factor acetylhydrolase (PAF-AH) in type 1 diabetes (DM1) patients treated with intensive insulin therapy. DESIGN AND METHODS:Studied parameters were measured...

journal_title：Clinical biochemistry

authors： Wegner M,Araszkiewicz A,Pioruńska-Mikołajczak A,Zozulińska-Ziółkiewicz D,Wierusz-Wysocka B,Pioruńska-Stolzmann M

更新日期：2009-11-01 00:00:00

abstract：:Congestive heart failure usually features a mild to moderate increase in aspartate aminotransferase (AST) activity. A marked increase in AST may occur rarely and is usually preceded by prolonged shock or hypotensive episodes and is accompanied by hyperbilirubinemia. I report two patients with congestive heart failure ...

journal_title：Clinical biochemistry

authors： Nanji AA

更新日期：1983-04-01 00:00:00

abstract：OBJECTIVES:Although numerous recent studies have focused on analyses of microRNA expression profiles in cancer cells, the expression patterns of the enzymes responsible for the generation of miRNAs remains largely unexplored. The purpose of this study was to investigate whether Dicer mRNA expression is altered during p...

journal_title：Clinical biochemistry

authors： Pampalakis G,Diamandis EP,Katsaros D,Sotiropoulou G

更新日期：2010-02-01 00:00:00

abstract：OBJECTIVES:We have previously identified three minor immunophilins of molecular weights 37 kDa, 14 kDa, and 5-8 kDa capable of binding tacrolimus and sirolimus. DESIGN AND METHODS:When tested against pure preparations of five sirolimus metabolites, the 14 kDa protein had almost no cross-reactivity, the 37 kDa protein ...

journal_title：Clinical biochemistry

authors： Davis DL,Murthy JN,Gallant-Haidner H,Yatscoff RW,Soldin SJ

更新日期：2000-02-01 00:00:00