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Activity and protein level of CuZnSOD and MnSOD in benign and malignant liver tumors.

Abstract：

OBJECTIVES:Superoxide dismutase (SOD) is a key antioxidant enzyme, responsible for scavenging of superoxide anion - a precursor of all reactive oxygen species (ROS). ROS are implicated in many pathologies, particularly in tumor disease. The aim of our work was to evaluate SOD isoforms' activity and protein level changes in liver tumors. DESIGN AND METHODS:Materials were obtained from patients with liver tumors and with liver cirrhosis diagnosed by routine histopathological examination. Activity and protein level of SOD were determined by means of the Beauchamp and Fridovich assay and by Western blot analysis. RESULTS:Decreased activity of CuZnSOD and MnSOD and distinct differences in SOD isoforms' protein expression in liver cirrhosis were found. Results also showed higher protein level and activity of SOD isoforms in liver malignant tumors than in benign ones. CONCLUSIONS:Malignant tumors have a better antioxidant system than benign ones. Moreover, weakening of antioxidant mechanisms and accumulation of oxidative damage in cirrhotic liver could initiate liver carcinogenesis.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Skrzycki M,Scibior D,Podsiad M,Czeczot H

doi

10.1016/j.clinbiochem.2007.09.014

subject

Has Abstract

pub_date

2008-01-01 00:00:00

pages

91-6

issue

1-2

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(07)00369-4

journal_volume

41

pub_type

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