Abstract:
OBJECTIVES:Familial hypercholesterolemia (FH) is characterized by increased oxidative stress (OS) levels. In the postprandial state, lipids and lipoproteins modulate OS status through their impact on pro-oxidant and antioxidant mechanisms. The objective of this study was to evaluate in patients with FH the response to an unsaturated oral fat load test (OFLT) by analyzing the mRNA levels of genes involved in the glutathione and thioredoxin antioxidant systems. DESIGN AND METHODS:We analyzed 14 FH patients and 20 normolipidemic and normoglycemic controls. In both groups, mRNA values of antioxidant enzyme genes (glutathione and thioredoxin systems) were determined at baseline and at 2, 4, 6, and 8h after OFLT by real time PCR. RESULTS:In the fasting state the mRNA levels of antioxidant enzymes GPX4 and the GSR, GSS, and GCLC enzymes (involved in glutathione regeneration and synthesis) and thioredoxin (TXN), were significantly increased in the FH group compared to the healthy controls. Some genes (GPX1 and GPX4) were increased at 4h in both groups, but values for the rest of the antioxidant enzyme mRNAs were decreased in FH patients after 4h from unsaturated OFLT and were increased in controls. CONCLUSIONS:We concluded that an OFLT with predominantly unsaturated fat has a different effect on postprandial antioxidant enzyme mRNA levels in controls than in FH patients. Increased antioxidant enzyme mRNA is not the main way to reduce postprandial oxidative stress in FH. This difference could determine the influence of dietary patterns in these patients.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Cortes R,Martinez-Hervas S,Ivorra C,De Marco G,Gonzalez-Albert V,Rojo-Martínez G,Saez G,Carmena R,Ascaso JF,Real JT,Chaves FJdoi
10.1016/j.clinbiochem.2014.09.006subject
Has Abstractpub_date
2014-12-01 00:00:00pages
291-7issue
18eissn
0009-9120issn
1873-2933pii
S0009-9120(14)00663-8journal_volume
47pub_type
杂志文章abstract:OBJECTIVES:There has been growing interest in the use of serum B-type natriuretic peptide (BNP) and the N-terminal segment of its pro-hormone (NT-proBNP) as biomarkers for cardiac disease. The aim of this review is to summarize the current state of knowledge regarding BNP and NT-proBNP measurement in the pediatric popu...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2005.12.004
更新日期:2006-06-01 00:00:00
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journal_title:Clinical biochemistry
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journal_title:Clinical biochemistry
pub_type: 杂志文章
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journal_title:Clinical biochemistry
pub_type: 杂志文章
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更新日期:2018-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1016/s0009-9120(00)00196-x
更新日期:2001-02-01 00:00:00
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更新日期:2009-07-01 00:00:00
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更新日期:2013-06-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2004.03.009
更新日期:2004-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-04-01 00:00:00
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更新日期:2010-08-01 00:00:00
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更新日期:2017-08-01 00:00:00
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pub_type: 杂志文章,多中心研究
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更新日期:1993-02-01 00:00:00
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journal_title:Clinical biochemistry
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更新日期:2010-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-12-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2004.12.010
更新日期:2005-05-01 00:00:00
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journal_title:Clinical biochemistry
pub_type: 杂志文章
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更新日期:2018-08-01 00:00:00
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journal_title:Clinical biochemistry
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更新日期:2018-11-01 00:00:00
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journal_title:Clinical biochemistry
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doi:10.1016/j.clinbiochem.2005.04.002
更新日期:2005-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1976-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:2018-07-01 00:00:00