Method for monitoring of the protein amino group changes during carbonylation.

Abstract:

OBJECTIVES:Carbonylation of the protein amino, guanidino and thiol groups is one of the important causes of vascular complications in diabetes. We developed a simple spectrophotometric method for monitoring of the changes in the protein amino group contents during carbonylation. DESIGN AND METHODS:The method is based on the reaction of amino group with p-benzoquinone in the slightly alkaline media. It was applied during carbonylation in vitro with methylglyoxal and in vivo in 13 patients with type 2 diabetes and 20 healthy persons. RESULTS:The method is simple, fast, precise (RSD in the range of 1.2-1.8%) and accurate (recovery about 100%). The content of amino groups in human serum albumin isolated from diabetics was significantly lower (p<0.01) in comparison with a control group. CONCLUSION:The method developed is suitable for quantification of protein amino groups during in vitro carbonylation as well as for clinical practice.

journal_name

Clin Biochem

journal_title

Clinical biochemistry

authors

Aćimović JM,Jovanović VB,Veselinović MR,Srećković VD,Mandić LM

doi

10.1016/j.clinbiochem.2011.05.019

subject

Has Abstract

pub_date

2011-08-01 00:00:00

pages

994-9

issue

12

eissn

0009-9120

issn

1873-2933

pii

S0009-9120(11)00370-5

journal_volume

44

pub_type

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