Activated KCNQ1 channel promotes fibrogenic response in hereditary gingival fibromatosis via clustering and activation of Ras.


BACKGROUND AND OBJECTIVE:Activated potassium channels were found to be strongly correlated with gingival overgrowth (GO) phenotype as we reviewed syndromic hereditary gingival fibromatosis (HGF). Nevertheless, the functional roles of potassium channels in gingival fibrosis or gingival overgrowth remained uncovered. The aim of the present study was to explore the pathogenic role of aberrantly activated potassium channel in Hereditary Gingival Fibromatosis (HGF). METHODS:Gingival tissues were collected from 9 HGF patients and 15 normal controls. Expression of KCNQ1 was detected by immunohistochemistry. Gingival fibroblasts were isolated, and outward K+ currents were detected by whole-cell patch-clamp analysis, transmembrane potential was determined by flow cytometry. Normal human gingival fibroblasts (NHGFs) were transfected with KCNQ1 adenovirus or treated with KCNQ1 selective agonist ML277 and antagonist chromanol 293B. Accumulation of Extracellular Matrix (ECM) was measured by Western blotting and Sircol Soluble Collagen Assay. Content of secreted TGF-β1 was measured by ELISA. Active RAS pull-down assay and cell immunofluorescence were utilized to verify RAS activation. RESULTS:KCNQ1 was upregulated in gingival tissues derived from HGF patients and HGF gingival fibroblasts presented increased outward K+ currents than NHGFs. Overexpression of KCNQ1, or KCNQ1 agonist ML277, promoted fibrotic responses of NHGFs. TGF-β1 and KCNQ1 channels formed a positive feed-back loop. ML277 generated lateral clustering and activation of Ras on plasma membrane, followed by augmented MAPK/AP-1 signaling pathway output. JNK or ERK1/2 inhibitors suppressed ML277-induced AP-1 and ECM upregulation. CONCLUSION:Activation of KCNQ1 potassium channel promoted fibrogenic responses in NHGFs via Ras/MAPK/AP-1 signaling.


J Periodontal Res


Gao Q,Yang C,Meng L,Wang Z,Chen D,Peng Y,Yang K,Bian Z




Has Abstract


2020-12-31 00:00:00






  • Effect of loading on the migration of periodontal fibroblasts in the rat incisor.

    abstract::The influence of occlusal loading on periodontal fibroblasts was investigated in hypoloaded (shortened out of occlusion), functionally loaded and hyperloaded (constant linguointrusive mechanical loads of 9.4 +/- 0.06 g) lower left rat incisors. One hour following injection of 3H-thymidine, half of the animals in each ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Michaeli Y,Shamir D,Weinreb M,Steigman S

    更新日期:1994-01-01 00:00:00

  • Alkaline phosphatase activity in human periodontal ligament: age effect and relation to cementum growth rate.

    abstract::Recently, a relationship was demonstrated between the thickness of the cementum layer in rat molars and the activity of alkaline phosphatase (ALP) in the adjoining periodontal ligament (PDL). It was the aim of the present study to investigate whether such a relationship also exists in the periodontium of man. Healthy ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: van den Bos T,Beertsen W

    更新日期:1999-01-01 00:00:00

  • Characterisation of black-pigmented anaerobes isolated from diseased and healthy periodontal sites.

    abstract::Prevotella intermedia has recently been re-defined and a new species, Prevotella nigrescens has been proposed. However, there is little data available on the incidence of these new species in periodontal health or disease. Black-pigmented anaerobes isolated from diseased and healthy subgingival sites were identified b...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Teanpaisan R,Douglas CW,Walsh TF

    更新日期:1995-07-01 00:00:00

  • Co-purified gelatinases alter the stability and biological activities of human plasma fibronectin preparations.

    abstract:BACKGROUND AND OBJECTIVE:Fibronectin (FN) is an important cell adhesion molecule that is used widely to characterize cell behavior. Preparations of FN purified from human plasma by gelatin-Sepharose affinity chromatography typically also contain gelatin-binding gelatinases that may cleave FN, reduce its stability and a...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Pal S,Chen Z,Xu X,Mikhailova M,Steffensen B

    更新日期:2010-04-01 00:00:00

  • Common target genes of palatal and gingival fibroblasts for EMD: the microarray approach.

    abstract:BACKGROUND AND OBJECTIVE:Connective tissue grafts are frequently applied, together with Emdogain(®) , for root coverage. However, it is unknown whether fibroblasts from the gingiva and from the palate respond similarly to Emdogain. The aim of this study was therefore to evaluate the effect of Emdogain(®) on fibroblasts...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Gruber R,Stähli A,Miron RJ,Bosshardt DD,Sculean A

    更新日期:2015-02-01 00:00:00

  • Expression and intracellular localization of progesterone receptors in cultured human gingival fibroblasts.

    abstract:OBJECTIVE:The aim of this immunocytochemical study was to characterize the expression and distribution of the progesterone receptor (PR) and estrogen receptor (ER) in gingival fibroblasts using culture cells derived from people at various ages. BACKGROUND:The reaction of female hormones is tissue or cell specific, and...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Kawahara K,Shimazu A

    更新日期:2003-06-01 00:00:00

  • A Salvia miltiorrhiza ethanol extract ameliorates tissue destruction caused by experimental periodontitis in rats.

    abstract:BACKGROUND AND OBJECTIVE:Salvia miltiorrhiza Bunge (Labiatae), or Danshen, is a Chinese medicine used for treatment of cardiac diseases by improving blood circulation and inhibiting inflammatory responses. In this study, we aimed to determine whether an ethanol extract of S. miltiorrhiza can ameliorate tissue damage ca...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Chiang CY,Tsai HT,Chang WL,Chin YT,Chang WJ,Tu HP,Chiu HC,Fu E

    更新日期:2016-02-01 00:00:00

  • Lipid peroxidation: a possible role in the induction and progression of chronic periodontitis.

    abstract:OBJECTIVES:Reactive oxygen species (ROS) are implicated in the destruction of the periodontium during inflammatory periodontal diseases. The imbalance in oxidant/antioxidant activity may be a key factor in the damaging effects of ROS. This study aimed to determine the lipid peroxidation levels in gingival crevicular fl...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Tsai CC,Chen HS,Chen SL,Ho YP,Ho KY,Wu YM,Hung CC

    更新日期:2005-10-01 00:00:00

  • Effects of periodontal non-surgical therapy plus azithromycin on glycemic control in patients with diabetes: a randomized clinical trial.

    abstract:BACKGROUND AND OBJECTIVE:Periodontitis may alter the systemic condition in patients with diabetes and hence interfere with glycemic control. The objective of this study was to determine the quantifiable changes in glycated hemoglobin (HbA1C) after periodontal non-surgical therapy plus azithromycin in a mixed population...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章,随机对照试验


    authors: Botero JE,Yepes FL,Ochoa SP,Hincapie JP,Roldan N,Ospina CA,Castrillon CA,Becerra MA

    更新日期:2013-12-01 00:00:00

  • Activation of cannabinoid receptor CB2 regulates osteogenic and osteoclastogenic gene expression in human periodontal ligament cells.

    abstract:BACKGROUND AND OBJECTIVE:Cannabinoid receptor CB2, expressed in osteoblasts and osteoclasts, plays a crucial role in the regulation of bone metabolism. Since periodontal ligament (PDL) cells can differentiate into osteoblasts, this study was undertaken to investigate CB2 expression and the effect of CB2 activation on o...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Qian H,Zhao Y,Peng Y,Han C,Li S,Huo N,Ding Y,Duan Y,Xiong L,Sang H

    更新日期:2010-08-01 00:00:00

  • Role of the junctional epithelium in periodontal innate defense and homeostasis.

    abstract:BACKGROUND AND OBJECTIVE:  The junctional epithelium provides the front-line defense against periodontal bacterial infection. The migration of neutrophils into the junctional epithelium might represent a protective reaction against bacterial infections. However, neutrophils penetrate into the junctional epithelium even...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Tsukamoto Y,Usui M,Yamamoto G,Takagi Y,Tachikawa T,Yamamoto M,Nakamura M

    更新日期:2012-12-01 00:00:00

  • Matrix metalloproteinases and their inhibitors in gingival mast cells in persons with and without human immunodeficiency virus infection.

    abstract:BACKGROUND:Mast cells are a prominent cell type in the gingival infiltrate in periodontitis. In this study we examined the expression by gingival mast cells of matrix metalloproteinases, MMP-1, MMP-2, MMP-8 and the tissue inhibitors of metalloproteinases, TIMP-1 and TIMP-2. METHODS:Gingival specimens from 12 human imm...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Naesse EP,Schreurs O,Helgeland K,Schenck K,Steinsvoll S

    更新日期:2003-12-01 00:00:00

  • Effect of model fitting artifacts on the stepwise approach to identifying patterns of attachment loss.

    abstract::The stepwise approach to the determination of periodontal attachment loss involves fitting linear, logarithmic, and exponential models to individual site data and concluding that the form of loss is consistent with the model that has the greatest r-value, provided that the model predicts loss in excess of a site-speci...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Cohen ME

    更新日期:1996-01-01 00:00:00

  • Analysis of interleukin-1beta-modulated mRNA gene transcription in human gingival keratinocytes by epithelia-specific cDNA microarrays.

    abstract:BACKGROUND/OBJECTIVES:Proinflammatory cytokines such as interleukin-1beta are known to be synthesized in oral gingivitis and periodontitis and lead to the activation of the transcription factor nuclear factor-kappaB (NF-kappaB). Although numerous effects of interleukin-1beta on mesenchymal cells are known, e.g. up-regu...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Steinberg T,Dannewitz B,Tomakidi P,Hoheisel JD,Müssig E,Kohl A,Nees M

    更新日期:2006-10-01 00:00:00

  • Repeated exposure to whole cigarette smoke promotes primary human gingival epithelial cell growth and modulates keratin expression.

    abstract:BACKGROUND AND OBJECTIVE:The gingiva is the first oral tissue directly exposed to cigarette smoke (CS). Exposure to CS compromises the structure and function of gingival tissue. Damaging or altering the gingival epithelium leads to a compromised protective barrier of the periodontium, resulting in several diseases. The...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Alharbi IA,Rouabhia M

    更新日期:2016-10-01 00:00:00

  • Crevicular fluid and serum IgG subclasses and corresponding mRNA expressing plasma cells in periodontitis lesions.

    abstract::Recent reports suggest that specific serum IgG subclasses are a feature of several forms of periodontitis. GCF antibodies are both serum-derived and locally produced by the abundant plasma cells of the diseased periodontal tissue. Previous work has shown that crevicular fluid (GCF) levels of IgG may be reduced in acti...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Kinane DF,Takahashi K,Mooney J

    更新日期:1997-01-01 00:00:00

  • Evidence that cyclosporine inhibits periodontal prostaglandin I2 synthesis.

    abstract::Cyclosporine (CsA) is a selective immunosuppressant widely used in clinical therapy. Like phenytoin and nifedipine, the drug is associated with gingival overgrowth. This study considers the interaction of CsA and prostaglandin I2 (PGI2), in particular the action of the drug on gingival tissue in vitro and in vivo. The...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Nell A,Matejka M,Solar P,Ulm C,Sinzinger H

    更新日期:1996-02-01 00:00:00

  • Differential reactivity of anti-E-selectin monoclonal antibodies in human gingival tissue.

    abstract::E-selectin is a cytokine-inducible endothelial glycoprotein which participates in the binding of leukocytes to vascular endothelium. Variable levels of expression of E-selectin have been reported in gingivitis and periodontitis, and two differing concepts of its significance have emerged: either gingival blood vessels...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Pietrzak ER,Savage NW,Xu LJ,Walsh LJ

    更新日期:1995-11-01 00:00:00

  • A fluid-phase endocytotic capacity and intracellular degradation of a foreign protein (horseradish peroxidase) by lysosomal cysteine proteinases in the rat junctional epithelium.

    abstract::We investigated the co-localization of lysosomal cathepsins B, H and L, and horseradish peroxidase (HRP) in junctional epithelial (JE) cells both as a fluid-phase endocytotic marker to demonstrate the fluid-phase endocytotic capacity of JE cells, and to understand the morphological relationships of the endocytosed for...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Yamaza T,Kido MA,Kiyoshima T,Nishimura Y,Himeno M,Tanaka T

    更新日期:1997-11-01 00:00:00

  • Implant osseointegration in irradiated bone: an experimental study.

    abstract:BACKGROUND AND OBJECTIVES:The aim of this study was to evaluate the effect of radiotherapy on the osseointegration of dental implants in the tibia and the action of platelet-rich plasma (PRP) as an adjuvant therapy. MATERIAL AND METHODS:A total of 18 rabbits received two implants in each tibial epiphysis, totalizing 7...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Ocaña RP,Rabelo GD,Sassi LM,Rodrigues VP,Alves FA

    更新日期:2017-06-01 00:00:00

  • Evaluation of physiological tooth movements within clinically normal periodontal tissues by means of periodontal pulsation measurements.

    abstract::The three-dimensional physiological tooth movement synchronized with heartbeat, periodontal pulsation, has already been demonstrated by previous researchers. The purpose of this study was to estimate physiological tooth movements within clinically normal periodontal tissues, thus establishing periodontal pulsation sta...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Ioi H,Morishita T,Nakata S,Nakasima A,Nanda RS

    更新日期:2002-04-01 00:00:00

  • Clinical application of autologous fibroblast cell culture in gingival recession treatment.

    abstract:BACKGROUND AND OBJECTIVE:Gingival recession is defined as soft and hard tissue displacement resulting in root surface exposure. The optimal outcome of gingival recession treatment is complete, predictable and long-lasting root coverage with a significant level of tissue regeneration. Tissue engineering, which applies a...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章,随机对照试验


    authors: Milinkovic I,Aleksic Z,Jankovic S,Popovic O,Bajic M,Cakic S,Lekovic V

    更新日期:2015-06-01 00:00:00

  • Effect of 1,25(OH)2 D3 and 20(OH)D3 on interleukin-1β-stimulated interleukin-6 and -8 production by human gingival fibroblasts.

    abstract:BACKGROUND AND OBJECTIVE:Vitamin D-1,25(OH)2 D3 or 1,25D3-maintains healthy osseous tissue, stimulates the production of the antimicrobial peptide cathelicidin and has anti-inflammatory effects, but it can cause hypercalcemia. Evidence links diminished serum levels of 1,25D3 with increased gingival inflammation. Period...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Nakashyan V,Tipton DA,Karydis A,Livada R,Stein SH

    更新日期:2017-10-01 00:00:00

  • Prepubertal periodontitis: a review of diagnostic criteria, pathogenesis, and differential diagnosis.

    abstract::This paper reviews the literature on prepubertal periodontitis (PP) according to the definition and the classification of PP by Page. The generalized form of PP (G-PP) defined by Page was identified as the oral manifestation of a systemic disease called leukocyte adhesion deficiency (LAD) and thus the pathogenesis is ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章,评审


    authors: Watanabe K

    更新日期:1990-01-01 00:00:00

  • (-)-Epigallocatechin gallate induces apoptosis, via caspase activation, in osteoclasts differentiated from RAW 264.7 cells.

    abstract:BACKGROUND AND OBJECTIVE:Alveolar bone resorption is a characteristic feature of periodontal diseases and involves removal of both the mineral and the organic constituents of the bone matrix, a process mainly carried out by multinucleated osteoclast cells. (-)-Epigallocatechin gallate, the main constituent of green tea...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Yun JH,Kim CS,Cho KS,Chai JK,Kim CK,Choi SH

    更新日期:2007-06-01 00:00:00

  • Immunohistochemical localization of epidermal growth factor receptors in human gingival epithelia.

    abstract::Epidermal growth factor (EGF) is a small molecular weight polypeptide which is thought to have important functions in epithelial growth and differentiation and in wound healing. EGF exerts its action on cells through binding to a cell surface receptor. Using immunohistochemistry and a monoclonal antibody (mAb) directe...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Nordlund L,Hormia M,Saxén L,Thesleff I

    更新日期:1991-07-01 00:00:00

  • Neutrophils in chronic and aggressive periodontitis in interaction with Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans.

    abstract:BACKGROUND AND OBJECTIVE:This study analyzed the interaction of Porphyromonas gingivalis ATCC 33277 and Aggregatibacter actinomycetemcomitans Y4 with peripheral blood polymorphonuclear neutrophils taken from patients with aggressive periodontitis and chronic periodontitis. MATERIAL AND METHODS:Peripheral blood polymor...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Guentsch A,Puklo M,Preshaw PM,Glockmann E,Pfister W,Potempa J,Eick S

    更新日期:2009-06-01 00:00:00

  • Regulation of oxidative-stress responsive genes by arecoline in human keratinocytes.

    abstract:BACKGROUND AND OBJECTIVE:Arecoline, an arecanut alkaloid present in the saliva of betel quid chewers, has been implicated in the pathogenesis of a variety of inflammatory oral diseases, including oral submucous fibrosis and periodontitis. To understand the molecular basis of arecoline action in epithelial changes assoc...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Thangjam GS,Kondaiah P

    更新日期:2009-10-01 00:00:00

  • Galectin-8 mediates fibrogenesis induced by cyclosporine in human gingival fibroblasts.

    abstract:BACKGROUND AND OBJECTIVE:During cyclosporine-induced gingival overgrowth, the homeostatic balance of gingival connective tissue is disrupted leading to fibrosis. Galectins are glycan-binding proteins that can modulate a variety of cellular processes including fibrosis in several organs. Here, we study the role of galec...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Smith PC,Metz C,de la Peña A,Oyanadel C,Avila P,Arancibia R,Vicuña L,Retamal C,Barake F,González A,Soza A

    更新日期:2020-10-01 00:00:00

  • The effects of beam hardening on digital subtraction radiography.

    abstract::Quantitative assessment of osseous changes attributable to periodontal disease is made possible by digital subtraction radiography. Tissues through which x rays travel to produce dental radiographs essential to this process alter the energy spectrum of the beam such that calibration errors result when densitometry is ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Webber RL,Tzukert A,Ruttimann U

    更新日期:1989-01-01 00:00:00