Activated KCNQ1 channel promotes fibrogenic response in hereditary gingival fibromatosis via clustering and activation of Ras.


BACKGROUND AND OBJECTIVE:Activated potassium channels were found to be strongly correlated with gingival overgrowth (GO) phenotype as we reviewed syndromic hereditary gingival fibromatosis (HGF). Nevertheless, the functional roles of potassium channels in gingival fibrosis or gingival overgrowth remained uncovered. The aim of the present study was to explore the pathogenic role of aberrantly activated potassium channel in Hereditary Gingival Fibromatosis (HGF). METHODS:Gingival tissues were collected from 9 HGF patients and 15 normal controls. Expression of KCNQ1 was detected by immunohistochemistry. Gingival fibroblasts were isolated, and outward K+ currents were detected by whole-cell patch-clamp analysis, transmembrane potential was determined by flow cytometry. Normal human gingival fibroblasts (NHGFs) were transfected with KCNQ1 adenovirus or treated with KCNQ1 selective agonist ML277 and antagonist chromanol 293B. Accumulation of Extracellular Matrix (ECM) was measured by Western blotting and Sircol Soluble Collagen Assay. Content of secreted TGF-β1 was measured by ELISA. Active RAS pull-down assay and cell immunofluorescence were utilized to verify RAS activation. RESULTS:KCNQ1 was upregulated in gingival tissues derived from HGF patients and HGF gingival fibroblasts presented increased outward K+ currents than NHGFs. Overexpression of KCNQ1, or KCNQ1 agonist ML277, promoted fibrotic responses of NHGFs. TGF-β1 and KCNQ1 channels formed a positive feed-back loop. ML277 generated lateral clustering and activation of Ras on plasma membrane, followed by augmented MAPK/AP-1 signaling pathway output. JNK or ERK1/2 inhibitors suppressed ML277-induced AP-1 and ECM upregulation. CONCLUSION:Activation of KCNQ1 potassium channel promoted fibrogenic responses in NHGFs via Ras/MAPK/AP-1 signaling.


J Periodontal Res


Gao Q,Yang C,Meng L,Wang Z,Chen D,Peng Y,Yang K,Bian Z




Has Abstract


2020-12-31 00:00:00






  • Single nucleotide polymorphisms in long noncoding RNA, ANRIL, are not associated with severe periodontitis but with adverse cardiovascular events among patients with cardiovascular disease.

    abstract:BACKGROUND AND OBJECTIVE:Biological plausibility of an association between severe periodontitis and cardiovascular disease (CVD) has been proven. Genetic characteristics play an important role in both complex inflammatory diseases. Polymorphisms (single nucleotide polymorphisms [SNPs]) in the long noncoding RNA, antise...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Schulz S,Seitter L,Werdan K,Hofmann B,Schaller HG,Schlitt A,Reichert S

    更新日期:2018-10-01 00:00:00

  • Correlation between periodontal disease, inflammatory alterations and pre-eclampsia.

    abstract:BACKGROUND AND OBJECTIVE:Several studies have hypothesized that periodontal disease may increase the risk of pre-eclampsia. The correlation between the two diseases would probably be based on hypertension-related cytokine release in the local periodontal environment. The aim of this study was to evaluate the associatio...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Politano GT,Passini R,Nomura ML,Velloso L,Morari J,Couto E

    更新日期:2011-08-01 00:00:00

  • Effect of 1,25(OH)2 D3 and 20(OH)D3 on interleukin-1β-stimulated interleukin-6 and -8 production by human gingival fibroblasts.

    abstract:BACKGROUND AND OBJECTIVE:Vitamin D-1,25(OH)2 D3 or 1,25D3-maintains healthy osseous tissue, stimulates the production of the antimicrobial peptide cathelicidin and has anti-inflammatory effects, but it can cause hypercalcemia. Evidence links diminished serum levels of 1,25D3 with increased gingival inflammation. Period...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Nakashyan V,Tipton DA,Karydis A,Livada R,Stein SH

    更新日期:2017-10-01 00:00:00

  • Galectin-8 mediates fibrogenesis induced by cyclosporine in human gingival fibroblasts.

    abstract:BACKGROUND AND OBJECTIVE:During cyclosporine-induced gingival overgrowth, the homeostatic balance of gingival connective tissue is disrupted leading to fibrosis. Galectins are glycan-binding proteins that can modulate a variety of cellular processes including fibrosis in several organs. Here, we study the role of galec...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Smith PC,Metz C,de la Peña A,Oyanadel C,Avila P,Arancibia R,Vicuña L,Retamal C,Barake F,González A,Soza A

    更新日期:2020-10-01 00:00:00

  • Fluoridation effects on periodontal disease among adults.

    abstract::Numerous studies report that water fluoridation reduces caries in children, but little current evidence exists about fluoridation's effects on the periodontal health of adults. To address this issue, we estimated fluoridation effects on periodontal disease among 1066 Washington state employees and their spouses, aged ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Grembowski D,Fiset L,Spadafora A,Milgrom P

    更新日期:1993-05-01 00:00:00

  • Five parameters of gingival crevicular fluid from eight surfaces in periodontal health and disease.

    abstract::Volume and amounts of myeloperoxidase (MPO), lactoferrin (LF), aryl sulfatase (AS) and lactate dehydrogenase (LDH) were measured in gingival crevicular fluid (GCF) collected from the mesial and distal proximal surfaces of the premolars and first and second molars of 3 subject groups. Group assignment was based on subj...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Smith QT,Au GS,Freese PL,Osborn JB,Stoltenberg JL

    更新日期:1992-09-01 00:00:00

  • The modulatory role of cementum matrix on osteoblastic cells in vitro.

    abstract::The formation of new cementum is an important issue in clinical periodontology, as cementum is required to provide attachment for newly formed periodontal tissues to the root surface. In this study a model of cementogenesis in vitro was used in order to test the effects of root surface demineralization on the migratio...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Tenorio D,Foyle DM,Hughes FJ

    更新日期:1997-05-01 00:00:00

  • Parotid secretory protein is expressed and inducible in human gingival keratinocytes.

    abstract:BACKGROUND:Parotid secretory protein (PSP) is a major salivary protein that is thought to possess both antibacterial and anti-inflammatory activity. A major question is whether PSP expression can be regulated by humoral factors and bacteria. Periodontitis is an inflammatory lesion initiated by interaction between gingi...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Shiba H,Venkatesh SG,Gorr SU,Barbieri G,Kurihara H,Kinane DF

    更新日期:2005-04-01 00:00:00

  • Increased oxygen exposure alters collagen expression and tissue architecture during ligature-induced periodontitis.

    abstract:BACKGROUND AND OBJECTIVE:The aim of this study was to evaluate the effects of increased oxygen availability on gene expression and on collagen deposition/maturation in the periodontium following disease. MATERIAL AND METHODS:Male Wistar rats had ligatures placed around their molars to induce periodontal disease, and a...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Gajendrareddy PK,Junges R,Cygan G,Zhao Y,Marucha PT,Engeland CG

    更新日期:2017-06-01 00:00:00

  • Isorhamnetin inhibits Prevotella intermedia lipopolysaccharide-induced production of interleukin-6 in murine macrophages via anti-inflammatory heme oxygenase-1 induction and inhibition of nuclear factor-κB and signal transducer and activator of transcript

    abstract:BACKGROUND AND OBJECTIVE:Interleukin-6 (IL-6) is a key proinflammatory cytokine that has been considered to be important in the pathogenesis of periodontal disease. Therefore, host-modulatory agents directed at inhibiting IL-6 appear to be beneficial in terms of attenuating periodontal disease progression and potential...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Jin JY,Choi EY,Park HR,Choi JI,Choi IS,Kim SJ

    更新日期:2013-12-01 00:00:00

  • Patients with chronic periodontitis present increased risk for osteoporosis: A population-based cohort study in Taiwan.

    abstract:OBJECTIVES:Chronic periodontitis is a bone destructive inflammatory disease with an adverse impact on general health and suggested underlying factors in common with osteoporosis. A few studies have examined the possible relationship between chronic periodontitis and osteoporosis; however, the results remain inconclusiv...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Mau LP,Kuan YC,Tsai YC,Lin JJ,Huynh-Ba G,Weng PW,Shieh YS,Cheng WC,Huang RY

    更新日期:2017-10-01 00:00:00

  • Gingival tissue, an extrasynovial source of malondialdehyde-acetaldehyde adducts, citrullinated and carbamylated proteins.

    abstract:BACKGROUND AND OBJECTIVE:Postranslational modification of proteins can lead to the production of autoantibodies and loss of immune tolerance. This process has been hypothesised to be a critical factor in the pathogenesis of rheumatoid arthritis. The objective of this study was to demonstrate that inflamed human gingiva...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Bright R,Thiele GM,Manavis J,Mikuls TR,Payne JB,Bartold PM

    更新日期:2018-02-01 00:00:00

  • Diabetes in the dental office: using NHANES III to estimate the probability of undiagnosed disease.

    abstract:BACKGROUND AND OBJECTIVE:Recent data have suggested that in the past 15 years there has been a dramatic increase in the incidence of diabetes mellitus in the USA. However, evidence suggests that approximately one-third of diabetes cases remain undiagnosed. Because 60% of Americans see a dentist at least once per year f...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Borrell LN,Kunzel C,Lamster I,Lalla E

    更新日期:2007-12-01 00:00:00

  • Impact of periodontal disease on quality of life: a systematic review.

    abstract::The diagnosis of periodontal disease is commonly based on objective evaluations of the patient's medical/dental history as well as clinical and radiographic examinations. However, periodontal disease should also be evaluated subjectively through measures that quantify its impact on oral health-related quality of life....

    journal_title:Journal of periodontal research

    pub_type: 杂志文章,评审


    authors: Ferreira MC,Dias-Pereira AC,Branco-de-Almeida LS,Martins CC,Paiva SM

    更新日期:2017-08-01 00:00:00

  • Expression of endothelin-1 in gingival epithelial cells.

    abstract:OBJECTIVES AND BACKGROUND:Endothelin-1 (ET-1) is a 21-amino acid peptide with multifunctional regulation. ET-1 expresses in various cells during inflammation. The present study aimed to examine the ET-1 expression in oral epithelial cells after infection with the periodontal pathogen and to investigate the presence of ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Yamamoto E,Awano S,Koseki T,Ansai T,Takehara T

    更新日期:2003-08-01 00:00:00

  • Detection of putative periodontal pathogens in non-insulin-dependent diabetes mellitus and non-diabetes mellitus by polymerase chain reaction.

    abstract::It has been assumed that there is a relationship between periodontal diseases and diabetes mellitus, however the putative periodontal microorganisms in non-diabetes mellitus (non-DM) individuals and non-insulin-dependent diabetes mellitus (NIDDM) patients have not been well studied. In this study, the detection rates ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Yuan K,Chang CJ,Hsu PC,Sun HS,Tseng CC,Wang JR

    更新日期:2001-02-01 00:00:00

  • The effect of ketoprofen creams on periodontal disease in rhesus monkeys.

    abstract::Ketoprofen creams were evaluated for the treatment of periodontal disease in a placebo-controlled, double-blind study in the rhesus monkeys, Macaca mulatta. Two formulations containing ketoprofen (1%), with or without vitamin E, were evaluated against appropriate controls (8 monkeys per group). Two weeks prior to trea...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Li KL,Vogel R,Jeffcoat MK,Alfano MC,Smith MA,Collins JG,Offenbacher S

    更新日期:1996-11-01 00:00:00

  • Significance of elevated gingival crevicular fluid tumor necrosis factor-alpha and interleukin-8 levels in chronic hemodialysis patients with periodontal disease.

    abstract:BACKGROUND AND OBJECTIVE:The prevalence of chronic renal disease in industrialized countries is increasing, and chronic renal disease and periodontitis can have significant, reciprocal effects. The aim of this study was to evaluate the associations between specific clinical parameters and the levels of tumor necrosis f...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Dağ A,Firat ET,Kadiroğlu AK,Kale E,Yilmaz ME

    更新日期:2010-08-01 00:00:00

  • Alpha-terpineol complexed with beta-cyclodextrin reduces damages caused by periodontitis in rats.

    abstract:BACKGROUND AND OBJECTIVE:This study aimed to assess the effectiveness of the treatment with alpha-terpineol (αTPN) complexed with beta-cyclodextrin (βCD) on oral, blood, and hepatic parameters in ligature-induced periodontitis. MATERIAL AND METHODS:Forty female rats were distributed among the following groups: control...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Vasconcelos ACCG,Vasconcelos DFP,da Silva FRP,França LFC,Alves EHP,Di Lenardo D,Pessoa LDS,Nascimento HMS,Carvalho ADS,Sousa FBM,Barbosa ALDR,Medeiros JR,Novaes PD,Mariano FS,Lima BDS,Araujo AAS,Júnior LJQ,de Oliveira A

    更新日期:2020-12-01 00:00:00

  • Implant osseointegration in irradiated bone: an experimental study.

    abstract:BACKGROUND AND OBJECTIVES:The aim of this study was to evaluate the effect of radiotherapy on the osseointegration of dental implants in the tibia and the action of platelet-rich plasma (PRP) as an adjuvant therapy. MATERIAL AND METHODS:A total of 18 rabbits received two implants in each tibial epiphysis, totalizing 7...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Ocaña RP,Rabelo GD,Sassi LM,Rodrigues VP,Alves FA

    更新日期:2017-06-01 00:00:00

  • Electron-immunocytochemistry of laminin and type-IV collagen in the junctional epithelium of rat molar gingiva.

    abstract::The present study was carried out to examine the ultrastructural localization of laminin and type-IV collagen in the junctional epithelium of rat molar gingiva by means of the indirect immunoperoxidase method. Intense laminin reaction occurred in both the internal and the external basal laminae. Laminin reaction produ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Sawada T,Yamamoto T,Yanagisawa T,Takuma S,Hasegawa H,Watanabe K

    更新日期:1990-11-01 00:00:00

  • Prepubertal periodontitis: a review of diagnostic criteria, pathogenesis, and differential diagnosis.

    abstract::This paper reviews the literature on prepubertal periodontitis (PP) according to the definition and the classification of PP by Page. The generalized form of PP (G-PP) defined by Page was identified as the oral manifestation of a systemic disease called leukocyte adhesion deficiency (LAD) and thus the pathogenesis is ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章,评审


    authors: Watanabe K

    更新日期:1990-01-01 00:00:00

  • Merkel-like cells in Malassez epithelium in the periodontal ligament of cats: an immunohistochemical, confocal-laser scanning and immuno electron-microscopic investigation.

    abstract::The cellular heterogeneity of Malassez epithelium (ME) residing in the periodontal ligament has recently been reported, and the presence and coexistence of the neuropeptides calcitonin gene-related peptide (CGRP), substance P (SP) and vasoactive intestinal peptide (VIP) in single cells in ME has been shown (1). Howeve...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Tadokoro O,Maeda T,Heyeraas KJ,Vandevska-Radunovic V,Kozawa Y,Hals Kvinnsland I

    更新日期:2002-12-01 00:00:00

  • Inhibitory effect of enamel matrix derivative on osteoblastic differentiation of rat calvaria cells in culture.

    abstract:BACKGROUND AND OBJECTIVE:The effect of enamel matrix derivative (EMD) on bone differentiation remains unclear. Transforming growth factor beta1 (TGF-beta1) is reported to be contained in EMD. The aim of this study was to clarify the effect of EMD on osteoblastic cell differentiation and the possible role of TGF-beta1. ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Hama H,Azuma H,Seto H,Kido J,Nagata T

    更新日期:2008-04-01 00:00:00

  • Cost-effective design for dental randomized clinical trials with longitudinal observations.

    abstract::In general, randomized clinical trials (RCT) in dentistry involve longitudinal observations. In such studies, the total cost is a function of the number of study subjects and visits, the study duration, and the type and number of examinations at each visit. In this paper, we derived the minimum cost design for longitu...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Hefti AF,Yang MC

    更新日期:1999-04-01 00:00:00

  • Early-predictors of marginal bone loss around morse taper connection implants loaded with single crowns: A prospective longitudinal study.

    abstract:BACKGROUND AND OBJECTIVE:Few studies estimated the joint effect of implant design (length and diameter), peri-implant, and occlusal variables on early marginal bone loss. The monitoring of these factors during the first year after implant loading may be effective in preventing early implant failure. This prospective lo...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Radaelli MTB,Federizzi L,Nascimento GG,Leite FRM,Boscato N

    更新日期:2020-04-01 00:00:00

  • Subcutaneous and continuous administration of lipopolysaccharide increases serum levels of triglyceride and monocyte chemoattractant protein-1 in rats.

    abstract:OBJECTIVES:Previous studies have suggested an association between chronic infection and cardiovascular disease, and combined infection/inflammation is frequently related with hypertriglyceridemia, which may be a risk factor of cardiovascular disease. In fact, experimental transient or intermittent administration of lip...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Uchiumi D,Kobayashi M,Tachikawa T,Hasegawa K

    更新日期:2004-04-01 00:00:00

  • Tensile strength, histological and immunohistochemical observations of periodontal wound healing in the dog.

    abstract::This study was designed to study wound strength at the dentine/connective tissue interface and at the bone/connective tissue interface following full thickness flap surgery. Flaps of uniform dimension were outlined in four young adult beagle dogs using a standardised double bladed knife and vertical incisions 10 mm ap...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Werfully S,Areibi G,Toner M,Bergquist J,Walker J,Renvert S,Claffey N

    更新日期:2002-10-01 00:00:00

  • Evaluation of physiological tooth movements within clinically normal periodontal tissues by means of periodontal pulsation measurements.

    abstract::The three-dimensional physiological tooth movement synchronized with heartbeat, periodontal pulsation, has already been demonstrated by previous researchers. The purpose of this study was to estimate physiological tooth movements within clinically normal periodontal tissues, thus establishing periodontal pulsation sta...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Ioi H,Morishita T,Nakata S,Nakasima A,Nanda RS

    更新日期:2002-04-01 00:00:00

  • Nicotine inhibits osteogenic differentiation of human periodontal ligament cells under cyclic tensile stress through canonical Wnt pathway and α7 nicotinic acetylcholine receptor.

    abstract:BACKGROUND AND OBJECTIVES:Nicotine, the main psychoactive component of tobacco, affects cell metabolism, proliferation, adhesion and, importantly, the osteogenic differentiation of fibroblasts. Approximately 15% of all orthodontic patients are adults among who one-fifth are smokers. Hence, it is necessary to have insig...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Yu W,Hu B,Shi X,Cao Z,Ren M,He Z,Lin J,Deng H,Hu R

    更新日期:2018-08-01 00:00:00