Abstract：
BACKGROUND:Parotid secretory protein (PSP) is a major salivary protein that is thought to possess both antibacterial and anti-inflammatory activity. A major question is whether PSP expression can be regulated by humoral factors and bacteria. Periodontitis is an inflammatory lesion initiated by interaction between gingival keratinocytes and periodontopathogenic microorganisms such as the Gram-negative anaerobe Porphyromonas gingivalis. Cytokines and sex hormones have been implicated in the progression of various forms of periodontal diseases. MATERIALS AND METHODS:We investigated the expression of PSP and its regulation in primary cultures of human gingival keratinocytes (HGK). HGK at the third or fourth passage were exposed to heat-killed P. gingivalis, tumor necrosis factor-alpha (TNF-alpha) and 17beta-estradiol. The PSP mRNA levels were examined by real-time polymerase chain reaction (PCR). The protein expression of PSP was confirmed by immunofluorescence. RESULTS:Heat-killed P. gingivalis, TNF-alpha and 17beta-estradiol all resulted in increased HGK levels of mRNA for PSP as determined by real-time PCR analysis. Immunofluorescence demonstrated increased PSP localized within the cytoplasm of HGK following exposure to killed P. gingivalis. CONCLUSION:The present study has demonstrated for the first time that PSP is expressed in keratinocytes and that it can be up-regulated by bacteria and humoral factors. Thus PSP may have a role in the innate defense system at the gingival epithelial surface.
journal_name
J Periodontal Resjournal_title
Journal of periodontal researchauthors
Shiba H,Venkatesh SG,Gorr SU,Barbieri G,Kurihara H,Kinane DFdoi
10.1111/j.1600-0765.2005.00781.xsubject
Has Abstractpub_date
2005-04-01 00:00:00pages
153-7issue
2eissn
0022-3484issn
1600-0765pii
JRE781journal_volume
40pub_type
杂志文章abstract：:We investigated the co-localization of lysosomal cathepsins B, H and L, and horseradish peroxidase (HRP) in junctional epithelial (JE) cells both as a fluid-phase endocytotic marker to demonstrate the fluid-phase endocytotic capacity of JE cells, and to understand the morphological relationships of the endocytosed for...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1997.tb00575.x
更新日期：1997-11-01 00:00:00
abstract：:Interleukin-8 (IL-8) is believed to play an important role in the pathogenesis of various forms of periodontitis. In addition, the anti-IL-8 autoantibody has been recently recognized as a potent modulator of IL-8 function. In the current study, the concentrations of IL-8 and its autoantibody in gingival crevicular flu...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1034/j.1600-0765.2003.02001.x
更新日期：2003-02-01 00:00:00
abstract：:The immunoglobulin class distribution of antibody to human collagen type I has been examined in sera and gingival extracts from patients with adult chronic periodontitis. Tissue extracts were made either by simple washing or ultrasonication. With either method, IgG and IgA antibodies to collagen were present in higher...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1992.tb02086.x
更新日期：1992-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Tobacco use is associated with increased periodontal destruction in both cigarette smokers and smokeless tobacco users. Gingival keratinocytes are the first cells in contact with microbial and tobacco components and play a key role in the innate immune response to these agents. The objective of...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2009.01262.x
更新日期：2010-08-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Periodontal ligament stem cells (PDLSCs) have potential for osteogenic differentiation and show a great foreground in treating bone diseases. Histone three lysine 27 (H3K27) demethylase lysine demethylase 6A (KDM6A) is a critical epigenetic modifier and plays an important role in regulating ost...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12830
更新日期：2020-12-24 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Statins are inhibitors of 3-hydroxy-3-methylglutaryl coenzyme A reductase and have anti-inflammatory effects independent of cholesterol lowering. Recent clinical studies have indicated that statin intake has a beneficial effect on periodontal disease. However, the underlying mechanisms have not...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12132
更新日期：2014-08-01 00:00:00
abstract：BACKGROUND:Alveolar bone resorption is a characteristic feature of periodontal diseases and involves the removal of both the mineral and organic constituents of the bone matrix, which is caused by either multinucleated osteoclast cells or matrix metalloproteinases (MMPs). The gram-negative bacterium, Porphyromonas ging...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2004.00743.x
更新日期：2004-10-01 00:00:00
abstract：:The titre and avidity of IgG antibodies to Porphyromonas gingivalis whole cells and a 47 kDa cell surface protein were determined in serum samples taken from 20 subjects with adult periodontitis and 20 controls, matched for age, gender, ethnic origin and oral hygiene status. Antibody titres were measured by ELISA and ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1997.tb01379.x
更新日期：1997-01-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:CYP1A1 rs1048943 polymorphism was reported to be correlated with periodontitis; however, its association with aggressive periodontitis (AgP) has not yet been investigated. The aim of the study was to investigate the association between the CYP1A1 gene rs1048943 variant with generalized aggressi...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12658
更新日期：2019-10-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Biological plausibility of an association between severe periodontitis and cardiovascular disease (CVD) has been proven. Genetic characteristics play an important role in both complex inflammatory diseases. Polymorphisms (single nucleotide polymorphisms [SNPs]) in the long noncoding RNA, antise...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12555
更新日期：2018-10-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Naringenin, a naturally occurring flavonoid, possesses a wide range of pharmacological properties. The purpose of this study was to investigate the effect of naringenin on human osteoclastogenesis and osteoclastic bone resorption. MATERIAL AND METHODS:Naringenin was tested in a human osteoclas...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2008.01107.x
更新日期：2009-04-01 00:00:00
abstract：:Effects of lathyrogens such as aminoacetonitrile (AAN), beta-aminopropionitrile (BAPN) and cysteamine--known inhibitors of cross-linking of collagen--on the mechanical strength of the periodontal ligament of the rat mandibular first molar were examined by measuring the ultimate load required to extract the tooth from ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1989.tb00880.x
更新日期：1989-09-01 00:00:00
abstract：OBJECTIVE:Smoking causes pathological changes in all tissues, including gingiva and alveolar bone. The aim of present study was to evaluate apoptotic tissue alterations and tissue destruction in smoker and non-smoker periodontitis patients and healthy individuals. METHODS:Gingival biopsy samples from 15 systemically a...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12723
更新日期：2020-06-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Alveolar bone resorption is a characteristic feature of periodontal diseases and involves removal of both the mineral and the organic constituents of the bone matrix, a process mainly carried out by multinucleated osteoclast cells. (-)-Epigallocatechin gallate, the main constituent of green tea...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2006.00935.x
更新日期：2007-06-01 00:00:00
abstract：:In the present study, the effect of bacterial protease on the periodontal tissues in rats was investigated histologically and histometrically. In the control group, 0.03 ml distilled water was applied to the lingual marginal gingiva of the mandibular incisor while, in the experimental group, 0.03 ml bacterial protease...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1989.tb02009.x
更新日期：1989-05-01 00:00:00
abstract：:This paper reviews the literature on prepubertal periodontitis (PP) according to the definition and the classification of PP by Page. The generalized form of PP (G-PP) defined by Page was identified as the oral manifestation of a systemic disease called leukocyte adhesion deficiency (LAD) and thus the pathogenesis is ...
journal_title：Journal of periodontal research
pub_type： 杂志文章,评审
doi：10.1111/j.1600-0765.1990.tb01205.x
更新日期：1990-01-01 00:00:00
abstract：:The characteristic hemagglutination (HA) of Leptotrichia buccalis was used for measuring its attachment to various human cells and for determining if saliva contained hemagglutination inhibition (HI) factors. The microbial strain utilized displayed the characteristic EM morphology of L. buccalis. Sonicated preparation...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1978.tb00196.x
更新日期：1978-09-01 00:00:00
abstract：:We have reported recently that increased expression of membrane alkaline phosphatase (ALP) activity is a phenotypical characteristic of gingival fibroblasts located in chronic inflammatory periodontal lesions. To understand the cellular properties of these cells, we isolated ALP-positive gingival fibroblasts from pati...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1996.tb00495.x
更新日期：1996-05-01 00:00:00
abstract：:The 3 human salivary cystatins S, SA and SN are multifunctional proteins that possess a cysteine protease inhibitory property, but their ability to act as such is very different (SN > SA > S). One form, S, also appears to possess antibacterial properties towards the bacterium Porphyromonas gingivalis, often associated...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1999.tb02279.x
更新日期：1999-11-01 00:00:00
abstract：BACKGROUND/OBJECTIVES:PTH plays an important role in bone remodeling, and different actions have been reported depending on its administration method. iPSCs are promising as a cell source for regeneration of periodontal tissue due to their ability of proliferation and pluripotency. However, the effects of PTH on iPSCs ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12762
更新日期：2020-10-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Regulation of epithelial cell behavior associated with periodontitis is not well elucidated but many responses will ultimately be regulated by growth factor receptors. Using a rat experimental periodontitis model, protein and gene expression of select growth factor receptors in junctional and p...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2006.00881.x
更新日期：2006-08-01 00:00:00
abstract：:Accumulating evidence indicates that TNFalpha plays an important role in the pathogenesis of periodontitis, but the effect of TNFalpha on the degradation of the periodontal ligament is not well understood. This study used reverse transcriptase-PCR to investigate the effects of TNFalpha on matrix metalloproteinase (MMP...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1034/j.1600-0765.2002.00656.x
更新日期：2002-06-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Neutrophil extracellular traps (NETs) are a recently discovered antimicrobial mechanism used by neutrophils that have been proposed as an intervention in the pathogenesis of periodontitis. The objective of our study was to characterize the expression of NETs in gingival tissues with periodontit...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/jre.12621
更新日期：2019-06-01 00:00:00
abstract：:Recently, nitric oxide (NO) has been shown to be vital in inflammatory processes. Nitric oxide synthase (NOS) exists in three different isoforms, two constitutively produced with physiological roles, and an inducible form, iNOS, which is involved in inflammation. This study examined the localisation of iNOS in biopsie...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1034/j.1600-0765.2000.035006369.x
更新日期：2000-12-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:Irsogladine maleate counters gap junctional intercellular communication reduction induced by interleukin-8 or Actinobacillus actinomycetemcomitans in cultured human gingival epithelial cells. Interleukin-1 beta is involved in periodontal disease. Little is known, however, about the effect of in...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2007.01000.x
更新日期：2008-02-01 00:00:00
abstract：:The stepwise approach to the determination of periodontal attachment loss involves fitting linear, logarithmic, and exponential models to individual site data and concluding that the form of loss is consistent with the model that has the greatest r-value, provided that the model predicts loss in excess of a site-speci...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1996.tb00458.x
更新日期：1996-01-01 00:00:00
abstract：:This umbrella review appraised existing systematic reviews and meta-analysis to establish the impact of periodontal disease and therapy on general and oral health-related quality of life. A systematic electronic literature search was carried out in accordance with the PRISMA guideline up to January 2020 using PubMed, ...
journal_title：Journal of periodontal research
pub_type： 杂志文章,meta分析,评审
doi：10.1111/jre.12805
更新日期：2021-01-01 00:00:00
abstract：:The effectiveness of an ideal antimicrobial agent depends on its ability to kill microbes with minimal toxicity to host cells. Depending on the treatment regimen, antimicrobial agents come into contact with host cells for various intervals of time. Sanguinarium (SANG), chlorhexidine (CHX) and tetracycline (TET) are 3 ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1997.tb00542.x
更新日期：1997-04-01 00:00:00
abstract：:Using an agar-skim milk gel method, over 300 oral bacterial isolates were tested for their ability to produce trypsin or chymotrypsin inhibitor. Eleven bacterial strains, including seven isolates identified as Bacteroides intermedius, were found to inhibit both proteolytic activities. The inhibitory factors of the 11 ...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.1991.tb01726.x
更新日期：1991-09-01 00:00:00
abstract：BACKGROUND AND OBJECTIVE:beta defensin antimicrobial peptides are important in epithelial innate immunity, and their differential expression is associated with periodontal diseases. The aims of this study were to determine the mRNA expression of human beta defensin-1 and -2 in the gingival tissue of patients with gingi...
journal_title：Journal of periodontal research
pub_type： 杂志文章
doi：10.1111/j.1600-0765.2006.00964.x
更新日期：2007-10-01 00:00:00
