MiR-153-3p inhibits osteogenic differentiation of periodontal ligament stem cells through KDM6A-induced demethylation of H3K27me3.


BACKGROUND AND OBJECTIVE:Periodontal ligament stem cells (PDLSCs) have potential for osteogenic differentiation and show a great foreground in treating bone diseases. Histone three lysine 27 (H3K27) demethylase lysine demethylase 6A (KDM6A) is a critical epigenetic modifier and plays an important role in regulating osteogenic differentiation. Multiple microRNAs have been found to play important roles in osteogenesis. The aim of this study was to explore the mechanisms underlying the roles of miR-153-3p and KDM6A in PDLSC osteogenesis. METHODS:The levels of the osteogenic markers alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2), and osteopontin (OPN) were measured by western blotting. Osteoblast activity and mineral deposition were detected by ALP and Alizarin red S (ARS) staining. The levels of miR-153-3p and KDM6A were measured by quantitative real-time PCR (qRT-PCR). A luciferase reporter assay was used to confirm the interaction between KDM6A and miR-153-3p. Gain-of-function and loss-of-function assays were performed to identify the roles of miR-153-3p and KDM6A in the osteogenic differentiation of PDLSCs. RESULTS:In osteogenic PDLSCs, the expression of KDM6A, ALP, Runx2, and OPN was upregulated, whereas that of miR-153-3p was downregulated. miR-153-3p downregulation or KDM6A overexpression promoted the osteogenic differentiation of PDLSCs, as demonstrated by increases in ALP activity, matrix mineralization, and ALP, Runx2, and OPN expression. KDM6A was confirmed to be a target of miR-153-3p, and KDM6A overexpression reversed the inhibitory effect of miR-153-3p mimic on PDLSC osteogenesis. KDM6A promoted ALP, Runx2, and OPN expression through the demethylation of H3K27me3 on the promoter regions of these genes. CONCLUSION:miR-153-3p inhibited PDLSC osteogenesis by targeting KDM6A and inhibiting ALP, Runx2, and OPN transcription. These findings provide latent hope for PDLSCs application in periodontal therapy.


J Periodontal Res


Jiang H,Jia P




Has Abstract


2020-12-24 00:00:00






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    abstract:BACKGROUND AND OBJECTIVE:Osteonecrosis of the jaw (ONJ) is associated with bisphosphonate (BP) therapy. BPs alter osteoblast production of mediators of osteoclastogenesis, including interleukin (IL)-6, RANKL and osteoprotegerin (OPG), a RANKL antagonist. This can inhibit bone turnover and lead to necrosis. There is lit...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Tipton DA,Seshul BA,Dabbous MKh

    更新日期:2011-02-01 00:00:00

  • Bradykinin-2 receptor-mediated release of 3H-arachidonic acid and formation of prostaglandin E2 in human gingival fibroblasts.

    abstract::Bradykinin stimulated production of prostaglandin E2 (PGE2) and the release of 3H-arachidonic acid by gingival fibroblasts in a time- and dose-dependent manner. The effect on PGE2 biosynthesis was seen already after 15 seconds and was maximal after 5 minutes. Several structurally unrelated inhibitors of arachidonic ac...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Modéer T,Ljunggren O,Lerner UH

    更新日期:1990-11-01 00:00:00

  • Isolation and characterization of a 180-kiloDalton salivary glycoprotein which mediates the attachment of Actinomyces naeslundii to human buccal epithelial cells.

    abstract::The adherence of Actinomyces naeslundii to human buccal mucosa is mediated by specific interactions between the bacterial cell surface fimbriae and complementary beta-linked galactoside receptors on the epithelial cell surface. The buccal mucosa and the bacteria that colonize its surface are constantly bathed in saliv...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Babu JP,Dabbous MK,Abraham SN

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  • Estimation of sensitivity and specificity of site-specific diagnostic tests.

    abstract::Clinical trials designed to estimate the sensitivity and specificity of periodontal diagnostic tests often use multiple sites per patient as experimental units of analyses. Since site-specific test results within a patient are dependent observations, a correlated binomial model should be employed to estimate the sensi...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Hujoel PP,Moulton LH,Loesche WJ

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  • A tumor necrosis factor-alpha antagonist inhibits inflammatory bone resorption induced by Porphyromonas gingivalis infection in mice.

    abstract:BACKGROUND:A tumor-necrosis factor-alpha (TNF-alpha) antagonist, the WP9QY peptide, was designed based on the crystal structure of the TNF-beta/TNF-receptor complex in order to overcome the disadvantages of macromolecules such as antibodies or soluble receptors by reducing the molecular size of TNF-alpha antagonists. I...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Suzuki Y,Aoki K,Saito H,Umeda M,Nitta H,Baron R,Ohya K

    更新日期:2006-04-01 00:00:00

  • Immunoglobulin G profiles in different forms of periodontitis.

    abstract:BACKGROUND:Races and Gm(23) allotypes can modify the serum levels of IgG subclasses. The average serum levels of IgG subclasses of African-Americans have been reported to be higher than those of Caucasians in both healthy young adults and patients with aggressive periodontitis; Gm(23)-positive subjects generally had hi...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Chung HY,Lu HC,Chen WL,Lu CT,Yang YH,Tsai CC

    更新日期:2003-10-01 00:00:00

  • Alpha-terpineol complexed with beta-cyclodextrin reduces damages caused by periodontitis in rats.

    abstract:BACKGROUND AND OBJECTIVE:This study aimed to assess the effectiveness of the treatment with alpha-terpineol (αTPN) complexed with beta-cyclodextrin (βCD) on oral, blood, and hepatic parameters in ligature-induced periodontitis. MATERIAL AND METHODS:Forty female rats were distributed among the following groups: control...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Vasconcelos ACCG,Vasconcelos DFP,da Silva FRP,França LFC,Alves EHP,Di Lenardo D,Pessoa LDS,Nascimento HMS,Carvalho ADS,Sousa FBM,Barbosa ALDR,Medeiros JR,Novaes PD,Mariano FS,Lima BDS,Araujo AAS,Júnior LJQ,de Oliveira A

    更新日期:2020-12-01 00:00:00

  • Immunolocalization of matrix metalloproteinases and TIMP-1 (tissue inhibitor of metalloproteinases) in human gingival tissues from periodontitis patients.

    abstract::The matrix metalloproteinases (MMPs) collagenase, gelatinase A (72 kDa gelatinase), stromelysin, and their specific inhibitor TIMP-1 (tissue inhibitor of metalloproteinases), were immunolocalized using specific polyclonal antisera in gingival tissues from 21 patients with chronic inflammatory periodontal disease. Mono...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Meikle MC,Hembry RM,Holley J,Horton C,McFarlane CG,Reynolds JJ

    更新日期:1994-03-01 00:00:00

  • Nicotine inhibits osteogenic differentiation of human periodontal ligament cells under cyclic tensile stress through canonical Wnt pathway and α7 nicotinic acetylcholine receptor.

    abstract:BACKGROUND AND OBJECTIVES:Nicotine, the main psychoactive component of tobacco, affects cell metabolism, proliferation, adhesion and, importantly, the osteogenic differentiation of fibroblasts. Approximately 15% of all orthodontic patients are adults among who one-fifth are smokers. Hence, it is necessary to have insig...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Yu W,Hu B,Shi X,Cao Z,Ren M,He Z,Lin J,Deng H,Hu R

    更新日期:2018-08-01 00:00:00

  • Co-purified gelatinases alter the stability and biological activities of human plasma fibronectin preparations.

    abstract:BACKGROUND AND OBJECTIVE:Fibronectin (FN) is an important cell adhesion molecule that is used widely to characterize cell behavior. Preparations of FN purified from human plasma by gelatin-Sepharose affinity chromatography typically also contain gelatin-binding gelatinases that may cleave FN, reduce its stability and a...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Pal S,Chen Z,Xu X,Mikhailova M,Steffensen B

    更新日期:2010-04-01 00:00:00

  • Porphyromonas gingivalis heat shock protein vaccine reduces the alveolar bone loss induced by multiple periodontopathogenic bacteria.

    abstract:OBJECTIVES:Heat shock protein (HSP) can be utilized as a vaccine to cross-protect against multiple pathogenic species. The present study was performed to evaluate Porphyromonas gingivalis heat shock protein 60 (HSP60) as a vaccine candidate to inhibit multiple bacteria-induced alveolar bone loss. MATERIAL AND METHODS:...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Lee JY,Yi NN,Kim US,Choi JS,Kim SJ,Choi JI

    更新日期:2006-02-01 00:00:00

  • Urokinase-plasminogen activator protects periodontal ligament fibroblast from oxidative induced-apoptosis and DNA damage.

    abstract:BACKGROUND AND OBJECTIVE:Urokinase-plasminogen activator (uPA) is a serine protease expressed at high basal level in normal gingival cervical fluid. Despite its known pathologic role in tissue proteolysis in periodontitis, little is known concerning uPA physiological function in oral tissue. Recent evidence in cancer c...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Ahmad Akhoundi MS,Rokn A,Bagheri R,Momeni N,Hodjat M

    更新日期:2018-10-01 00:00:00

  • Long-term exposure of human gingival fibroblasts to cigarette smoke condensate reduces cell growth by modulating Bax, caspase-3 and p53 expression.

    abstract:BACKGROUND AND OBJECTIVE:Smoking cigarettes increases the risk of oral tissue damage leading to periodontal disease. Gingival fibroblasts, the predominant cell type inhabiting gingival connective tissue, play a critical role in remodeling and maintaining gingival structure. The objective of this study was to investigat...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Alamri A,Semlali A,Jacques É,Alanazi M,Zakrzewski A,Chmielewski W,Rouabhia M

    更新日期:2015-08-01 00:00:00

  • Cytolethal distending toxin of Actinobacillus actinomycetemcomitans. Occurrence and association with periodontal disease.

    abstract::The cytolethal distending toxin (Cdt) of Actinobacillus actinomycetemcomitans, a periodontal pathogen, is a newly described cytotoxin with immunosuppressive properties, capable of causing cell cycle arrest of lymphocytes. The objectives of this study were to investigate the occurrence of A. actinomycetemcomitans with ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Tan KS,Song KP,Ong G

    更新日期:2002-08-01 00:00:00

  • Isorhamnetin inhibits Prevotella intermedia lipopolysaccharide-induced production of interleukin-6 in murine macrophages via anti-inflammatory heme oxygenase-1 induction and inhibition of nuclear factor-κB and signal transducer and activator of transcript

    abstract:BACKGROUND AND OBJECTIVE:Interleukin-6 (IL-6) is a key proinflammatory cytokine that has been considered to be important in the pathogenesis of periodontal disease. Therefore, host-modulatory agents directed at inhibiting IL-6 appear to be beneficial in terms of attenuating periodontal disease progression and potential...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Jin JY,Choi EY,Park HR,Choi JI,Choi IS,Kim SJ

    更新日期:2013-12-01 00:00:00

  • Lipid peroxidation: a possible role in the induction and progression of chronic periodontitis.

    abstract:OBJECTIVES:Reactive oxygen species (ROS) are implicated in the destruction of the periodontium during inflammatory periodontal diseases. The imbalance in oxidant/antioxidant activity may be a key factor in the damaging effects of ROS. This study aimed to determine the lipid peroxidation levels in gingival crevicular fl...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Tsai CC,Chen HS,Chen SL,Ho YP,Ho KY,Wu YM,Hung CC

    更新日期:2005-10-01 00:00:00

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    abstract:BACKGROUND AND OBJECTIVE:Aggregatibacter actinomycetemcomitans is involved in oral and systemic infections, and is associated with, eg aggressive forms of periodontitis and with endocarditis. The cagE gene encodes a ≈39 kDa putative exotoxin expressed by A. actinomycetemcomitans. The level of conservation of cagE, and ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Johansson A,Claesson R,Höglund Åberg C,Haubek D,Oscarsson J

    更新日期:2017-10-01 00:00:00

  • Detection of putative periodontal pathogens in non-insulin-dependent diabetes mellitus and non-diabetes mellitus by polymerase chain reaction.

    abstract::It has been assumed that there is a relationship between periodontal diseases and diabetes mellitus, however the putative periodontal microorganisms in non-diabetes mellitus (non-DM) individuals and non-insulin-dependent diabetes mellitus (NIDDM) patients have not been well studied. In this study, the detection rates ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Yuan K,Chang CJ,Hsu PC,Sun HS,Tseng CC,Wang JR

    更新日期:2001-02-01 00:00:00

  • Interleukin-1 and interleukin-8 in nicotine- and lipopolysaccharide-exposed gingival keratinocyte cultures.

    abstract:BACKGROUND AND OBJECTIVE:Tobacco use is associated with increased periodontal destruction in both cigarette smokers and smokeless tobacco users. Gingival keratinocytes are the first cells in contact with microbial and tobacco components and play a key role in the innate immune response to these agents. The objective of...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Johnson GK,Guthmiller JM,Joly S,Organ CC,Dawson DV

    更新日期:2010-08-01 00:00:00

  • Effect of cell donor age on the synthetic properties of fibroblasts obtained from phenytoin-induced gingival hyperplasia.

    abstract::Diploid fibroblasts obtained from explants of human gingiva and maintained in vitro undergo a several-fold decrease in protein and collagen synthesis as a function of increasing donor age. Using drug-induced gingival hyperplasia as a model, we performed experiments to learn whether fibroblasts derived from hyperplasti...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


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  • Predominant immunoreactivity of Porphyromonas gingivalis heat shock protein in autoimmune diseases.

    abstract:BACKGROUND AND OBJECTIVE:Autoimmune diseases, including atherosclerosis, diabetes mellitus and rheumatoid arthritis, can be triggered and aggravated by the pathogen-driven antigenic peptide from Porphyromonas gingivalis HSP60. P. gingivalis is the major pathogen of chronic periodontitis, which is a global epidemic prev...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Jeong E,Lee JY,Kim SJ,Choi J

    更新日期:2012-12-01 00:00:00

  • Tanshinone IIA treatment alleviated the rat gingival connective tissue overgrowth induced by cyclosporine A.

    abstract:BACKGROUND AND OBJECTIVE:The use of cyclosporine A induces fibrous enlargement of the gingival connective tissue. Existing treatment modalities, although effective, do not necessarily prevent the recurrence of the lesion. Emerging evidence indicates that tanshinone IIA (Tan IIA) could effectively attenuate a variety of...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Ma S,Liu W,Liu P,Liu J,Chen L,Qin C

    更新日期:2016-10-01 00:00:00

  • Correlation between periodontal disease, inflammatory alterations and pre-eclampsia.

    abstract:BACKGROUND AND OBJECTIVE:Several studies have hypothesized that periodontal disease may increase the risk of pre-eclampsia. The correlation between the two diseases would probably be based on hypertension-related cytokine release in the local periodontal environment. The aim of this study was to evaluate the associatio...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Politano GT,Passini R,Nomura ML,Velloso L,Morari J,Couto E

    更新日期:2011-08-01 00:00:00

  • Differences in the developmental origins of the periosteum may influence bone healing.

    abstract:BACKGROUND AND OBJECTIVE:The jaw bone, unlike most other bones, is derived from neural crest stem cells, so we hypothesized that it may have different characteristics to bones from other parts of the body, especially in the nature of its periosteum. The periosteum exhibits osteogenic potential and has received consider...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Ichikawa Y,Watahiki J,Nampo T,Nose K,Yamamoto G,Irie T,Mishima K,Maki K

    更新日期:2015-08-01 00:00:00

  • Effects of periodontal non-surgical therapy plus azithromycin on glycemic control in patients with diabetes: a randomized clinical trial.

    abstract:BACKGROUND AND OBJECTIVE:Periodontitis may alter the systemic condition in patients with diabetes and hence interfere with glycemic control. The objective of this study was to determine the quantifiable changes in glycated hemoglobin (HbA1C) after periodontal non-surgical therapy plus azithromycin in a mixed population...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章,随机对照试验


    authors: Botero JE,Yepes FL,Ochoa SP,Hincapie JP,Roldan N,Ospina CA,Castrillon CA,Becerra MA

    更新日期:2013-12-01 00:00:00

  • Surface characteristics of cells from different layers of keratinized and non-keratinized oral epithelia.

    abstract::Portions of clinically healthy non-keratinized and keratinized oral epithelia were removed from adult vervet monkeys (Cercopithecus aethiops). The epithelium was separated from the underlying lamina propria by trypsin digestion, following which the epithelial cells were separated from the various epithelial layers. ...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Cleaton-Jones P

    更新日期:1975-05-01 00:00:00

  • Regulation of epithelial cell growth factor receptor protein and gene expression using a rat periodontitis model.

    abstract:BACKGROUND AND OBJECTIVE:Regulation of epithelial cell behavior associated with periodontitis is not well elucidated but many responses will ultimately be regulated by growth factor receptors. Using a rat experimental periodontitis model, protein and gene expression of select growth factor receptors in junctional and p...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Ekuni D,Firth JD,Putnins EE

    更新日期:2006-08-01 00:00:00

  • Interleukin-1 genotypes and the association between periodontitis and cardiovascular disease.

    abstract::An epidemiological association between periodontitis and cardiovascular disease has been reported in multiple studies. Various mechanisms have been proposed as potential explanations for this association, including a common factor that predisposes certain individuals to a hyper-responsive inflammatory response. Variat...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Kornman KS,Pankow J,Offenbacher S,Beck J,di Giovine F,Duff GW

    更新日期:1999-10-01 00:00:00

  • Butyrate, a bacterial metabolite, induces apoptosis and autophagic cell death in gingival epithelial cells.

    abstract:BACKGROUND AND OBJECTIVE:Butyrate is produced by some types of anaerobic periodontal bacteria. Millimolar concentrations of butyrate are found in mature dental plaque from periodontitis patients. Although butyrate reportedly has a variety of effects in many mammalian cells, its effect on gingival epithelial cells is no...

    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Tsuda H,Ochiai K,Suzuki N,Otsuka K

    更新日期:2010-10-01 00:00:00

  • Significance of elevated gingival crevicular fluid tumor necrosis factor-alpha and interleukin-8 levels in chronic hemodialysis patients with periodontal disease.

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    journal_title:Journal of periodontal research

    pub_type: 杂志文章


    authors: Dağ A,Firat ET,Kadiroğlu AK,Kale E,Yilmaz ME

    更新日期:2010-08-01 00:00:00