Abstract:
BACKGROUND AND OBJECTIVE:Smoking cigarettes increases the risk of oral tissue damage leading to periodontal disease. Gingival fibroblasts, the predominant cell type inhabiting gingival connective tissue, play a critical role in remodeling and maintaining gingival structure. The objective of this study was to investigate the effect of long-term exposure to cigarette smoke on human gingival fibroblast survival/apoptosis and the molecular pathways involved in these cell responses. MATERIAL AND METHODS:Human gingival fibroblasts were extracted from healthy non-smokers and cultured in the presence of cigarette smoke condensate (CSC). At the end of each time point, cell growth was evaluated by means of MTT assay. Apoptotic and necrotic gene's expression was investigated by polymerase chain reaction array and by annexin V/propidium iodide staining and cell cycle assays. Western blot was used to investigate Bax and p53 proteins. These tests were supported by caspase 3 activity analyses. RESULTS:High levels of CSC decreased cell growth and deregulated cell cycle progression by increasing the G(0)/G(1) and reducing the S and G(2)/M phases of the gingival fibroblasts. Polymerase chain reaction arrays revealed the activation of several apoptotic genes by CSC, including TNF receptors, caspases, Bax and p53. This was supported by increases in the Bax and p53 protein levels as well as by an elevated activity of caspase-3 in the CSC-exposed cells. Immunofluorescence staining demonstrated that both Bax and caspase-3 displayed a cytosolic and mitochondrial distribution in the CSC-exposed gingival fibroblasts, compared to controls. The damaging effect of CSC on gingival fibroblast growth was also supported by the decrease in interleukin 6 and 8 secretion by the gingival fibroblasts. CONCLUSION:These results suggest that CSC may contribute to deregulating fibroblast functions. This can compromise fibroblast-epithelial cell interactions, which ultimately increases the risk of gingival tissue damage and the onset of periodontitis.
journal_name
J Periodontal Resjournal_title
Journal of periodontal researchauthors
Alamri A,Semlali A,Jacques É,Alanazi M,Zakrzewski A,Chmielewski W,Rouabhia Mdoi
10.1111/jre.12223subject
Has Abstractpub_date
2015-08-01 00:00:00pages
423-33issue
4eissn
0022-3484issn
1600-0765journal_volume
50pub_type
杂志文章abstract:BACKGROUND AND OBJECTIVE:Activated potassium channels were found to be strongly correlated with gingival overgrowth (GO) phenotype as we reviewed syndromic hereditary gingival fibromatosis (HGF). Nevertheless, the functional roles of potassium channels in gingival fibrosis or gingival overgrowth remained uncovered. The...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12836
更新日期:2020-12-31 00:00:00
abstract:OBJECTIVE:To describe the periodontal conditions among 30-39- and 50-59-year-old rural Thais from the Province of Songkhla, Southern Thailand. METHODS:A total of 359 dentate persons were given a clinical examination comprising recordings of plaque and calculus in six teeth, and bleeding on probing, attachment level an...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2003.00367.x
更新日期:2003-04-01 00:00:00
abstract:OBJECTIVE AND BACKGROUND:To evaluate the association between periodontal disease, tooth loss and coronary heart disease (CHD). There is still controversy about the relationship between periodontal disease and tooth loss with vessel obstruction assessed using coronary angiography. MATERIAL AND METHODS:This cross-sectio...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12301
更新日期:2016-04-01 00:00:00
abstract:BACKGROUND/OBJECTIVES:PTH plays an important role in bone remodeling, and different actions have been reported depending on its administration method. iPSCs are promising as a cell source for regeneration of periodontal tissue due to their ability of proliferation and pluripotency. However, the effects of PTH on iPSCs ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12762
更新日期:2020-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Salivary proteomics technology can be used to evaluate the disease progression of periodontitis and the systemic screening of proteomes of saliva from subjects with aggressive periodontitis has not been available. The objective of this preliminary study was to compare the proteomic profile of w...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2008.01172.x
更新日期:2009-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Enamel matrix derivative (EMD) is used clinically to promote periodontal tissue regeneration, and it has been reported that EMD can induce the formation of osteoclasts in mouse marrow cultures. In the present study, we investigated the mechanisms of EMD-induced osteoclast formation using a mous...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2005.00868.x
更新日期:2006-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:To ascertain whether interdental cleaning behaviours of Australian adults were associated with lower levels of plaque, gingivitis and periodontal disease. MATERIAL AND METHODS:Data were obtained from the National Survey of Adult Oral Health 2004-06. Outcome variables were three indicators of o...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01420.x
更新日期:2012-04-01 00:00:00
abstract::Four rough-surfaced (R) and three smooth-surfaced (S) clinical isolates of Capnocytophaga obtained from the subgingival plaque of periodontitis patients were studied for their peptidase and protease profiles. The results were compared with those obtained with C. gingivalis (which has a smooth morphology). All cell ext...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1991.tb01621.x
更新日期:1991-01-01 00:00:00
abstract::Polymorphonuclear neutrophils (PMNs) are essential in host defense against periodontopathic bacteria such as Porphyromonas gingivalis. The uptake of immunoglobulin G (IgG)-opsonized bacteria via IgG Fc receptors (Fc gamma R) on PMN constitutes a central defense mechanism in periodontium. Fc gamma RIIIb is the most abu...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2000.035002065.x
更新日期:2000-04-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Cyclosporine A treatment is important in the therapy of a number of medical conditions; however, alveolar bone loss is an important negative side-effect of this drug. As such, we evaluated whether concomitant administration of simvastatin would minimize cyclosporine A-associated alveolar bone l...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2008.01143.x
更新日期:2009-08-01 00:00:00
abstract:OBJECTIVE:The aim of this immunocytochemical study was to characterize the expression and distribution of the progesterone receptor (PR) and estrogen receptor (ER) in gingival fibroblasts using culture cells derived from people at various ages. BACKGROUND:The reaction of female hormones is tissue or cell specific, and...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2003.00654.x
更新日期:2003-06-01 00:00:00
abstract::The role of bacteria in the initiation of periodontitis is well-documented and the end result, destruction of the alveolar bone and periodontal connective tissue, is readily observed; but the events occurring between these two points in time remain obscure and are the focus of this paper. Bacteria induce tissue destru...
journal_title:Journal of periodontal research
pub_type: 杂志文章,评审
doi:10.1111/j.1600-0765.1991.tb01649.x
更新日期:1991-05-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Metabolic syndrome (MetS) is a combination of risk factors (e.g. impaired glucose tolerance, hypertension, and dyslipidaemia) that significantly contribute to the development of cardiovascular diseases. The aim of the study was to compare the effects of nonsurgical periodontal treatment (NSPT) ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12328
更新日期:2016-08-01 00:00:00
abstract::The goal of a single step therapy has been an important consideration in the development of guided tissue regeneration devices. It would spare the patient from the need for repeated surgery and eliminate many problems associated with a non-resorbable barrier. Animal studies of a collagen membrane extracted from porcin...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1998.tb02196.x
更新日期:1998-07-01 00:00:00
abstract:OBJECTIVE:The responsiveness of the sympathetic nervous system (SNS) and the hypothalamic--pituitary--adrenal (HPA) axis plays a major role in immune regulation and for the outcome of infections and inflammatory disorders. This study was designed to investigate whether chemical SNS denervation with the noradrenaline-se...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2005.00803.x
更新日期:2005-08-01 00:00:00
abstract::The influence of occlusal loading on periodontal fibroblasts was investigated in hypoloaded (shortened out of occlusion), functionally loaded and hyperloaded (constant linguointrusive mechanical loads of 9.4 +/- 0.06 g) lower left rat incisors. One hour following injection of 3H-thymidine, half of the animals in each ...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1994.tb01087.x
更新日期:1994-01-01 00:00:00
abstract::Vascularization of the periodontal ligament was examined in developing upper first molars of rats from 5 to 30 d after birth with light and scanning electron microscopy. Formation of the vascular network in the periodontal ligament (PDL) started with the beginning of root formation. The PDL vessels derived from the ba...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1034/j.1600-0765.2000.035004201.x
更新日期:2000-08-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The field of salivary diagnostics lacks an accepted and validated biomarker of alveolar bone remodeling. To address this, we examined levels of salivary biomolecules specifically associated with biological aspects of bone remodeling in subjects with chronic periodontitis in a case-control study...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01445.x
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The use of cyclosporine A induces fibrous enlargement of the gingival connective tissue. Existing treatment modalities, although effective, do not necessarily prevent the recurrence of the lesion. Emerging evidence indicates that tanshinone IIA (Tan IIA) could effectively attenuate a variety of...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12335
更新日期:2016-10-01 00:00:00
abstract::The periodontium is a highly hierarchically organized organ composed of gingiva, alveolar bone, periodontal ligament and cementum. Periodontitis leads to the destruction of hard and soft tissues ultimately leading to a loss of the teeth supporting apparatus. Current treatments are capable of limiting the disease progr...
journal_title:Journal of periodontal research
pub_type: 杂志文章,评审
doi:10.1111/jre.12729
更新日期:2020-06-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Vitamin D-1,25(OH)2 D3 or 1,25D3-maintains healthy osseous tissue, stimulates the production of the antimicrobial peptide cathelicidin and has anti-inflammatory effects, but it can cause hypercalcemia. Evidence links diminished serum levels of 1,25D3 with increased gingival inflammation. Period...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12452
更新日期:2017-10-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:For ethical reasons it is becoming increasingly more difficult to obtain, from clinical studies, histological data on infrabony defects treated with guided tissue regeneration (GTR) techniques. The aim of this systematic review was to find the value of extrapolating animal data on treatment of ...
journal_title:Journal of periodontal research
pub_type: 杂志文章,meta分析,评审
doi:10.1111/jre.12130
更新日期:2014-08-01 00:00:00
abstract::The aim of the present study was to analyze the cytotoxicity of some bacterial species associated with periodontal diseases. The specificity of cytotoxicity was estimated against cells of various origin and from different individuals. The reference bacteria were Actinobacillus actinomycetemcomitans, Porphyromonas ging...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1996.tb01412.x
更新日期:1996-10-01 00:00:00
abstract::E-selectin is a cytokine-inducible endothelial glycoprotein which participates in the binding of leukocytes to vascular endothelium. Variable levels of expression of E-selectin have been reported in gingivitis and periodontitis, and two differing concepts of its significance have emerged: either gingival blood vessels...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1995.tb01290.x
更新日期:1995-11-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:Enamel matrix proteins are involved in the development and regeneration of root cementum and in its attachment to dentin; however, the mechanisms through which this occurs have yet to be elucidated. The present study was therefore carried out to evaluate the mitogenic and proliferative response...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2010.01313.x
更新日期:2011-02-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:It has been suggested that aggressive periodontitis has a genetic basis. Monocyte chemoattractant protein 1 (MCP-1) plays a critical role in the recruitment of monocytes and the development of periodontitis. The -2518MCP-1(A/G) polymorphism has been implicated as a risk or susceptibility factor...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2011.01426.x
更新日期:2012-06-01 00:00:00
abstract::Multinucleated cells (MNCs) that appeared after hydroxyapatite (HAP) implantation into experimentally-produced bone defects in rat periodontal tissues were investigated both ultrastructurally and ultracytochemically. At day 5 after implantation, MNCs first appeared along the HAP surface. They had no features of typica...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1992.tb02085.x
更新日期:1992-01-01 00:00:00
abstract:BACKGROUND AND OBJECTIVE:The origin of cells in the mature periodontium, and the location of their progenitors, are still unknown. It is also unknown whether inflammation influences the number and distribution of these cells within the periodontium. Molecules such as STRO-1, CD146 and CD44 have been identified on a var...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.2006.00904.x
更新日期:2006-12-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to evaluate the effects of non-surgical periodontal therapy on gingival crevicular fluid levels of matrix metalloproteinase-8 (MMP-8), interleukin-6 (IL-6) and prostaglandin E2 (PGE2 ) in patients with rheumatoid arthritis (RA) with periodontal disease. MATERIAL AND METHODS:Twenty-s...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/jre.12337
更新日期:2016-10-01 00:00:00
abstract::The characteristic hemagglutination (HA) of Leptotrichia buccalis was used for measuring its attachment to various human cells and for determining if saliva contained hemagglutination inhibition (HI) factors. The microbial strain utilized displayed the characteristic EM morphology of L. buccalis. Sonicated preparation...
journal_title:Journal of periodontal research
pub_type: 杂志文章
doi:10.1111/j.1600-0765.1978.tb00196.x
更新日期:1978-09-01 00:00:00