Abstract：
:An unfractionated heparin (UFH) and a depolymerised derivative of low molecular weight heparin (LMWH) have been compared for their ability to activate platelets suspended in citrated plasma (PRP) or after washing and suspension in hepes buffered tyrode containing fibrinogen. Neither heparin alone induced aggregation of washed platelets, but UFH and to a much lesser extent LMWH, induced aggregation of platelets in PRP. Both heparins caused significant enhancement of a low concentration of ADP-induced activation of PRP and, again, the effect of LMWH was somewhat less than that of UFH. UFH produced a marked potentiation of ADP-induced activation of washed platelets and LMWH was about a third as potent. In addition, UFH induced a potentiation of PAF-induced aggregation and dense-granule release in PRP, a property not shared by LMWH. In PRP, UFH was three times more potent at inhibiting thrombin-induced aggregation and dense-granule release, as might be expected from their specific activities in the KCCT and thrombin time assay. However, with washed platelets, both heparins were equivalent at inhibiting thrombin-induced aggregation, dense-granule release and elevation of cytosolic free calcium ([Ca++]i) as monitored by quin 2 fluorescence. UFH and LMWH alone did not induce a change in [Ca++]i, nor had they any effect on ADP- or PAF-induced elevation of [Ca++]i.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Westwick J,Scully MF,Poll C,Kakkar VVdoi
10.1016/0049-3848(86)90207-0subject
Has Abstractpub_date
1986-05-15 00:00:00pages
435-47issue
4eissn
0049-3848issn
1879-2472journal_volume
42pub_type
杂志文章abstract：:A nephelometric method is described for determination of plasminogen and two types of plasmin inhibitors in human plasma having different affinity toward plasmin. This method is based on the kinetic analysis of effects of whole plasma and plasmin inhibitor fraction obtained from plasma on the activity of exogenously a...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(83)90395-x
更新日期：1983-04-01 00:00:00
abstract：INTRODUCTION:Pre-eclampsia is an important cause of maternal morbidity and mortality. Its etiology is still unknown. Clinical symptoms correlate with activation of coagulation and inherited thrombophilia has been associated with pre-eclampsia. ABO blood group has been associated with thrombotic disorders and pre-eclamp...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2008.11.012
更新日期：2009-06-01 00:00:00
abstract：:Fasting plasma homocysteine level and the related clinical findings were analysed in 240 consecutive patients with venous thromboembolism. Hyperhomocysteinemia, defined as a plasma level above 20 micromol/l (corresponding to the percentile 95th in the controls), was present in 11.2% of the patients. Plasma homocystein...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(02)00096-8
更新日期：2002-04-15 00:00:00
abstract：:Anticoagulants used for platelet function studies in vitro may affect platelet responsiveness. In the present study we compared the influence of three different anticoagulants, sodium citrate, low molecular mass heparin, and recombinant hirudin, on platelet aggregation in whole blood in vitro, using impedance aggregom...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00114-x
更新日期：1997-07-01 00:00:00
abstract：:The enzymatic activities of uPA, and a collagenase-like proteinase in the post-nuclear fraction of cell homogenates of a metastatic carcinomatous cell line following X-ray irradiation were examined by the use of chromogenic substrates and by casein- or gelatin-containing zymographies and electrophoretic gel stained wi...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(94)90214-3
更新日期：1994-06-15 00:00:00
abstract：:Fibrinogen is a hexameric glycoprotein consisting of two sets of three polypeptides (the Aα, Bβ, and γ chains, encoded by the three genes FGA, FGB, and FGG). It is involved in the final phase of the coagulation process, being the precursor of the fibrin monomers necessary for the formation of the hemostatic plug. Rare...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.05.007
更新日期：2015-07-01 00:00:00
abstract：:Many patients with diabetes mellitus show increased platelet aggregation and prostaglandin synthesis in response to physiological agents such as ADP and collagen when their platelets are tested in platelet-rich plasma or washed platelet suspensions. However, the relationship between increased platelet aggregation in v...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90265-9
更新日期：1987-06-01 00:00:00
abstract：:The extracellular nucleotides, ATP and ADP, as well as adenosine have been implicated in a great number of physiological functions. ADP is one of the major platelet recruiting factors, whereas ATP is considered to be a competitive inhibitor of ADP-induced platelet aggregation and adenosine is able to induce vasodilata...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2007.01.006
更新日期：2007-01-01 00:00:00
abstract：:Owing to the beneficial health effects on human cardiovascular system, soybeans and soy-related products have been a focus of intensive research. Soy isoflavones are known to be primarily responsible for the soy-related biological effects including anti-platelet activity but its in vivo relevancy has not been fully ve...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2013.01.002
更新日期：2013-03-01 00:00:00
abstract：:The thrombin inhibitory role of antithrombin III (ATIII) and heparin cofactor II (HCII) was studied in vitro using intact and injured rabbit aortae. When intact vessels were loaded with thrombin and then exposed to either heat defibrinogenated human plasma (HDHP) or ATIII the same degree of thrombin inhibition was ach...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(91)90014-n
更新日期：1991-06-01 00:00:00
abstract：:Tissue factor (TF) is a transmembrane protein which, in complex with factor (F)VIIa, initiates blood coagulation. Numerous studies have determined TF epitopes and individual amino acids which play an important role in the TF/FVIIa complex formation and its activity towards natural substrates. However the subject of ce...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2012.02.022
更新日期：2012-05-01 00:00:00
abstract：:An in vitro test system was developed to examine the effects of red blood cells (RBC) on shear-induced platelet adhesion (SIPAD) and platelet aggregation (SIPAG). Suspensions of human platelets labeled with Mepacrine and suspended in citrated plasma were exposed to single, continuous or repetitive (120-300x) one secon...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(98)00160-1
更新日期：1998-12-15 00:00:00
abstract：:Atherothrombosis is the leading cause of morbidity and mortality in patients with diabetes mellitus. Several mechanisms contribute to the diabetic prothrombotic state, including endothelial dysfunction, coagulative activation and platelet hyper-reactivity. In particular, diabetic platelets are characterised by dysregu...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2011.11.052
更新日期：2012-03-01 00:00:00
abstract：:To explore social aspects of asymptomatic carriership of factor V Leiden mutation (FVL) and how carriers have experienced procedure of screening for FVL, we have performed a qualitative study using semi-structured interviews. Seventeen carriers of FVL without history of venous thromboembolism (VTE) were interviewed. C...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章
doi：10.1016/j.thromres.2004.02.002
更新日期：2004-01-01 00:00:00
abstract：BACKGROUND:Ambient particulate matter (PM) is associated with cardiovascular morbidity and mortality. It has been proposed that PM induces a pro-thrombotic process, increasing the risk of cardiovascular events, with some support from epidemiological and laboratory-based models. Diesel exhaust is a major contributor to ...
journal_title：Thrombosis research
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.thromres.2007.01.005
更新日期：2007-01-01 00:00:00
abstract：:The protease inhibitor, aprotinin, has been examined for its ability to inhibit urokinase and tissue-type plasminogen activators at pH 7.4 in assays utilizing pyroGlu-Gly-Arg-p-nitroanilide and H-D-Ile-Pro-Arg-p-nitroanilide as substrates, respectively. Aprotinin inhibited both two-chain low molecular weight urokinase...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(88)90252-6
更新日期：1988-03-15 00:00:00
abstract：:Collagen-induced platelet aggregation and secretion (ATP-release) in whole-blood and platelet-rich plasma was compared. Although the amount of ATP released was comparable in whole-blood and platelet-rich plasma, the maximum extent of aggregation was greater when platelets were studied in their natural milieu, i.e. who...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90203-x
更新日期：1989-03-01 00:00:00
abstract：:Calcium accumulating vesicles were prepared from washed human platelets. Ca2+ uptake could be stimulated with calmodulin. The stimulation of Ca2+ uptake was not abolished by the adenylate cyclase inhibitor 2',5'-dideoxyadenosine, thus excluding enhanced cAMP formation as a possible cause for the calmodulin effect. Our...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(86)91697-x
更新日期：1986-02-15 00:00:00
abstract：INTRODUCTION:Profuse postpartum hemorrhage (PPH) and red blood cell (RBC) transfusion have been suggested to be associated with venous thromboembolic events (VTE). However, it is not fully clear if they are independent major risk factors. METHODS:Women who gave birth in the Stockholm area between 1999 and 2002 were th...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2018.03.002
更新日期：2018-05-01 00:00:00
abstract：:Experimental disseminated intravascular coagulation (DIC) can be induced by 4-h sustained infusion of endotoxin in a dose of 100 mg/kg in rats. The experimental model of DIC in rats was used to study the preventive effect of dipyridamole against DIC. Before the infusion of endotoxin, 0.5, 5.0 or 50.0 mg/kg of dipyrida...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(83)90216-5
更新日期：1983-03-15 00:00:00
abstract：INTRODUCTION:Type 2 diabetes mellitus (T2DM) patients have a variable response profile to the P2Y(12) receptor antagonist clopidogrel. P2Y(12) receptor signalling promotes platelet procoagulant activity. The aim of this study was to determine if T2DM patients with suboptimal clopidogrel response have greater platelet p...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2008.10.001
更新日期：2009-07-01 00:00:00
abstract：:Forty women undergoing surgery under general anaesthesia for hyperplasia mammae were randomized to treatment with low dose heparin (5000 IU twice daily) or not. Preoperatively, and repeated on the 3. postoperative day, assays of euglobulin clot lysis time (ELT) after venous stasis, tissue plasminogen activator (t-PA) ...
journal_title：Thrombosis research
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0049-3848(87)90318-5
更新日期：1987-03-01 00:00:00
abstract：BACKGROUND:Serious bleeding is a frequent and feared treatment complication in patients treated with oral anticoagulants (OACs). Levels of von Willebrand factor (VWF) antigen have been linked to the risk of bleeding complications, mortality, and cardiovascular events. OBJECTIVES:In this longitudinal cohort study of ev...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/j.thromres.2015.08.016
更新日期：2015-11-01 00:00:00
abstract：:Chronic thromboembolic pulmonary hypertension (CTEPH) is the most severe long term complication of acute pulmonary embolism (PE). Untreated, CTEPH is associated with a very poor prognosis and high risk of mortality, although curation can be achieved by surgical removal of the obstructive endothelialised thromboemboli ...
journal_title：Thrombosis research
pub_type： 杂志文章,评审
doi：10.1016/j.thromres.2016.12.026
更新日期：2017-03-01 00:00:00
abstract：:To estimate the effect of dilazep in preventing restenosis after a transluminal angioplasty, we have attempted an animal experiment in which the efficacy of drug was tested on an angioplastic injury superimposed on to atherosclerotic lesions. Using a modification of Block's method, atherosclerotic lesions first were m...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(89)90122-9
更新日期：1989-06-15 00:00:00
abstract：:Whole blood electrical aggregometry (WBEA) has become an accepted method to gain quick information on platelet disorders. Compared to the optical method WBEA is closer to physiology and less complicated, but on the other hand more difficult to standardize. Different approaches have been attempted in the past to improv...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(90)90232-2
更新日期：1990-12-15 00:00:00
abstract：:We investigated the age-related changes in blood coagulation, fibrinolysis, and platelet aggregation in male WBN/Kob rats, animals that exhibit spontaneously diabetes mellitus at more than 6 months of age. The rats aged 6 months or more showed significant hyperglycemia, hypoinsulinemia, and hyperlipidemia. As changes ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(00)00196-1
更新日期：2000-06-15 00:00:00
abstract：:Antithrombin (AT) is a serpin capable of trapping thrombin (IIa) in a stable and covalent complex. Complex formation is prevented by leukocyte elastase (LE) cleavage near the AT reactive centre. We mutated the known LE cleavage sites of AT to explore the possibility of producing an LE-resistant AT molecule. Initially,...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/s0049-3848(97)00228-4
更新日期：1997-10-15 00:00:00
abstract：:We have studied the synthesis and subcellular distribution of the glycosylated membrane protein tissue factor (TF) in human blood monocytes and the inducible monocyte like cell line, HL-60. Following induction with endotoxin or the phorbol ester, TPA, tissue factor specific activity was measured in intact cells, sonic...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90405-1
更新日期：1987-11-15 00:00:00
abstract：:Thrombus formation was electrically induced in the left common carotid artery of rats with preoccluded both vertebral and right carotid arteries. Following thrombus induction, animals became unresponsive and lost their righting reflex. The loss of righting reflex was observed when cortical tissular pO2 reached 55 +/- ...
journal_title：Thrombosis research
pub_type： 杂志文章
doi：10.1016/0049-3848(87)90340-9
更新日期：1987-10-01 00:00:00
