The concomitant augmentation of urokinase-type plasminogen activator and collagenase-like proteinase activities in X-ray irradiated cells of a human metastatic carcinomatous line.

Abstract:

:The enzymatic activities of uPA, and a collagenase-like proteinase in the post-nuclear fraction of cell homogenates of a metastatic carcinomatous cell line following X-ray irradiation were examined by the use of chromogenic substrates and by casein- or gelatin-containing zymographies and electrophoretic gel stained with avidin-conjugated peroxidase. Enhanced activities were observed in these cells, while those of 5'nucleotidase and Na(+)-K(+)-ATPase were attenuated. A partial purification and characterization of the collagenase showed that it was able to hydrolyze the heat-denatured type-I collagen more efficiently than the native one. The activation of both uPA and collagenase enables an efficient degradation of matrix barrier proteins. These findings suggest that following a certain dose range of X-ray irradiation, tumor cells may increase their ability to migrate and invade through the enhancement of uPA and collagenase activities.

journal_name

Thromb Res

journal_title

Thrombosis research

authors

Uchida N,Takahashi K,Kasai T,Ishida T,Kwaan HC

doi

10.1016/0049-3848(94)90214-3

subject

Has Abstract

pub_date

1994-06-15 00:00:00

pages

565-76

issue

6

eissn

0049-3848

issn

1879-2472

journal_volume

74

pub_type

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