Abstract:
:We have studied the synthesis and subcellular distribution of the glycosylated membrane protein tissue factor (TF) in human blood monocytes and the inducible monocyte like cell line, HL-60. Following induction with endotoxin or the phorbol ester, TPA, tissue factor specific activity was measured in intact cells, sonicated cells, isolated plasma membranes and shed vesicles. We have shown that TF is transported over time from the cytoplasm to the plasma membrane and finally to shed membrane vesicles. Optimal transport of TF to the plasma membrane and shedding in membrane vesicles required glycosylation as judged by partial inhibition of this process by the tunicamycin homologues B2 and C2.
journal_name
Thromb Resjournal_title
Thrombosis researchauthors
Bona R,Lee E,Rickles Fdoi
10.1016/0049-3848(87)90405-1subject
Has Abstractpub_date
1987-11-15 00:00:00pages
487-500issue
4eissn
0049-3848issn
1879-2472journal_volume
48pub_type
杂志文章abstract:INTRODUCTION:Thrombomodulin (TM) has been described as a marker of endothelial injury in atherosclerosis. The role of TM as a predictor of PAD severity is to be proven. The goal of the present study is to compare the level of plasmatic (TMp) in patients with intermittent claudication with patients with critical ischemi...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2005.03.010
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND:The Clauss fibrinogen method and thrombin clotting time (TCT) are still routinely used in patients with cirrhosis to define fibrinogen concentration and clotting potential. The thromboelastometric functional fibrinogen FIBTEM assay evaluates the strength of fibrin-based clots in whole blood, providing inform...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2015.04.003
更新日期:2015-06-01 00:00:00
abstract:AIM:Postprandial platelet activation was related to postprandial insulin rather than glucose levels in a previous meal insulin study in type 2 diabetes mellitus (T2DM). We therefore compared postprandial platelet activation in type 1 (T1DM) patients without insulin secretion and T2DM patients with high postprandial ins...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2016.03.009
更新日期:2016-05-01 00:00:00
abstract::Cardiovascular diseases are one of the leading causes of morbidity and mortality in industrialized countries, and although many processes play a role in the development of vascular disease, thrombosis is the primary event that precipitates stroke and acute coronary syndromes. The blood platelets are of significant imp...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2011.09.026
更新日期:2012-02-01 00:00:00
abstract::The mechanistic paradigm underlying venous thrombosis at atypical locations stems from the observation that most events occur as a result of pathology of the organ system drained by the involved venous segment. Cerebral venous sinus thrombosis stands apart as an exception to this general rule. Although brain and sinus...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2010.04.015
更新日期:2010-08-01 00:00:00
abstract:INTRODUCTION:Congenital factor V (FV) deficiency is a rare inherited disorder. Three compound heterozygous missense mutations, Asp68His, His147Arg, and Arg2074Cys, were observed in a Taiwanese patient with moderately severe FV deficiency. METHOD:The novel His147Arg mutation in the A1 domain was investigated by protein...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.04.005
更新日期:2014-07-01 00:00:00
abstract::The role of Protein C in combined factor V/VIII deficiency was examined by reducing the Protein C concentration using warfarin therapy in a patient with the combined deficiency. The factor VIII deficiency was like Hemophilia-A, with deficiency of VIII:C and VIII:C(Ag), but normal VIIIR:Ag and VIIIR:cof. The factor V d...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90188-9
更新日期:1984-12-15 00:00:00
abstract::Anticoagulant fucoidan fractions of different molecular weight and sulfate content were prepared and investigated for their effects on platelet function in vitro. The fucoidan fractions were incubated with human platelet rich plasma (PRP) at concentrations of 5, 10 and 50 micrograms/ml. Platelet activation was subsequ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(97)00037-6
更新日期:1997-03-15 00:00:00
abstract:BACKGROUND:In patients who are receiving warfarin therapy and require cataract surgery, it may be possible to continue warfarin in the perioperative period but the safety of this management strategy has not been systematically evaluated. METHODS:We performed a systematic review of the literature to assess the safety (...
journal_title:Thrombosis research
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.thromres.2009.01.007
更新日期:2009-07-01 00:00:00
abstract::Since the 19(th) century an association between cancer and thromboembolic events has been described, with a poorer survival prognosis. Production and secretion of procoagulant factors affect tumor biology and cancer-associated thrombosis. Tissue factor (TF) exerts coagulant and protease activated receptor (PAR)-depend...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/S0049-3848(14)50013-8
更新日期:2014-05-01 00:00:00
abstract::Experimental and clinical studies support the pivotal role played by reactive oxidant species in the mechanism of platelet activation. This effect is achieved via multiple pathways, including enhanced formation of isoprostanes. Platelet NADPH oxidase seems to be the main source of platelet reactive oxidant species and...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2011.12.002
更新日期:2012-03-01 00:00:00
abstract::Despite clear potential benefits of outpatient care, most patients suffering from pulmonary embolism (PE) are currently hospitalized due to the fear of possible adverse events. Nevertheless, some teams have increased or envisage to increase outpatient treatment or early discharge. We performed a narrative systematic r...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2017.05.001
更新日期:2017-07-01 00:00:00
abstract::Dietary intake of fish oils, rich in the polyunsaturated fatty acids (PUFAs) docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), has given inconsistent results as to their influence on the plasma fibrinogen level (1, 2, 3, 4, 5, 6). In the present study we have examined the effects of various fatty acids, the ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(95)00152-h
更新日期:1995-10-01 00:00:00
abstract::Murine monoclonal antibodies (mAb) were raised to a purified product of bovine PF-4, a 9,500 dalton protein with heparin neutralization activity comparable to that of human PF-4. Using a non-radioactive slide immunoenzymatic assay, four major classes of mAb could be identified when comparisons were made between purifi...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(86)90384-1
更新日期:1986-03-15 00:00:00
abstract:INTRODUCTION:Plasminogen activator inhibitor (PAI-1) may have an independent prognostic value in breast cancer (BC). PAI-1 4G/5G polymorphism may have significance for antigen expression. Thus, we analyzed the possible associations between PAI-1 4G/5G polymorphism, plasma PAI-1 levels, and clinicopathological features ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2009.02.014
更新日期:2009-09-01 00:00:00
abstract:OBJECTIVE:The present cohort study was performed to investigate the impact of the factor 5 rs6025 [F5] and the factor 2 rs1799963 [F2] mutations on high-titer inhibitor development [HRI] in patients with severe/moderate-severe hemophilia A [HA]. PATIENTS AND METHODS:216 patients with F8<2% born between 1980 and 2011 w...
journal_title:Thrombosis research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.thromres.2014.01.005
更新日期:2014-04-01 00:00:00
abstract::Factor IX Nagoya 3 (IX Nagoya 3) is a natural mutant of factor IX recognized in a patient with moderately severe hemophilia B. The patient had 0.60 units/ml of factor IX antigen and 2-5% of clotting activity. IX Nagoya 3 was purified from the patient's plasma by immunoaffinity chromatography with an anti-factor IX mon...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(90)90109-p
更新日期:1990-11-15 00:00:00
abstract:INTRODUCTION:It is currently unclear whether chronic kidney disease (CKD) and the decrease in renal function can influence the risk of venous thromboembolism (VTE) recurrence. MATERIALS AND METHODS:We performed an ambispective observational study on 409 patients with a previous episode of VTE. All the patients were in...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2017.10.011
更新日期:2017-12-01 00:00:00
abstract::The two objectives of the study were to determine whether a procedure could be developed for measuring cancer procoagulant (CP) activity in human serum and if this procedure provided a method for distinguishing people with cancer from those without cancer. A procedure was developed for processing human serum such that...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(89)90256-9
更新日期:1989-11-01 00:00:00
abstract::Thrombolysis is an effective but potential deleterious therapy and should therefore be limited to patients with acute intracerebral vessel occlusion and salvageable tissue. MRI currently develops towards the new diagnostic standard for the selection of stroke patients eligible for acute thrombolytic treatment and acut...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/s0049-3848(01)00309-7
更新日期:2001-09-30 00:00:00
abstract::Effects of mechanical trauma of platelets and red blood cells on dynamic viscoelasticity of blood during clotting were examined. Two different methods were attempted to give the mechanical damage to blood cells. In one method, a flow apparatus consisting of a thin teflon tube and two sample reservoirs connecting to bo...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(86)90084-8
更新日期:1986-08-15 00:00:00
abstract:INTRODUCTION:There is some controversy regarding the effect of CYP2C19 polymorphism on clinical outcome in patients with dual antiplatelet therapy. Chronic kidney disease (CKD) is associated with increased risk of cardiovascular event, but the association between the possession of CYP2C19 loss-of-function (LOF) alleles...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.07.039
更新日期:2014-11-01 00:00:00
abstract:OBJECTIVE:The diallelic glycoprotein IIIa polymorphism P1A1/A2 was attributed to be an inherited risk factor for coronary events. Whether this polymorphism affects response to aspirin in patients with coronary artery disease is not known. METHODS:We assessed thrombin generation (prothrombin fragment F1+2) in consecuti...
journal_title:Thrombosis research
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.thromres.2006.03.005
更新日期:2007-01-01 00:00:00
abstract::A prolongation of the thrombin clotting time by at least 25% was found in the presence of 8 of 26 IgG myeloma proteins, 2 of 5 IgA proteins and 1 of 10 macroglobulins, in a concentration of 10 mg/ml. Normal pooled immunoglobulin in concentrations up to 83 mg/ml were inactive. 2 IgG and 2 IgM monoclonal immunoglobulins...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(84)90315-3
更新日期:1984-07-01 00:00:00
abstract:BACKGROUND:Many heparin-induced thrombocytopenia (HIT) antibodies cause platelet activation in the serotonin release assay (SRA) in the absence of heparin. This in vitro observation may help unravel the mechanism of delayed-onset HIT, where seropositive patients develop thrombocytopenia and associated thrombosis after ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2009.10.021
更新日期:2010-04-01 00:00:00
abstract::The present investigation has used an aggregation-disaggregation-reaggregation model to determine if exposure to potent aggregating agents causing a refractory state in deaggregated platelets results in down-regulation and clearance of GPIb/IX, the receptor for von willebrand factor. Thrombin, the thrombin receptor ac...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/s0049-3848(96)00185-5
更新日期:1996-11-15 00:00:00
abstract::Red blood cells have historically been viewed as innocent bystanders in the process of blood coagulation and thrombin generation; however a century of clinical evidence linking red blood cells to thrombosis suggests the contrary. In this brief review, the biochemical evidence for red blood cell involvement in thrombin...
journal_title:Thrombosis research
pub_type: 杂志文章,评审
doi:10.1016/j.thromres.2013.01.023
更新日期:2013-05-01 00:00:00
abstract::Hemorrhagic and thrombotic complications are common in the term and preterm infant. Limited information is currently available about neonatal platelet structure and function, and how these may predispose infants to bleeding problems. This study comparing platelet ultrastructure of 71 different term and preterm infants...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/0049-3848(94)90039-6
更新日期:1994-03-15 00:00:00
abstract:INTRODUCTION:D-dimer, a fibrin degradation product, is related to risk of cardiovascular disease and venous thromboembolism. Genetic determinants of D-dimer are not well characterized; notably, few data have been reported for African American (AA), Asian, and Hispanic populations. MATERIALS AND METHODS:We conducted a ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2014.05.018
更新日期:2014-08-01 00:00:00
abstract::The zymogen form of circulating Factor VII activating protease (FSAP) is activated by histones that are released as a consequence of tissue damage or excessive inflammation. This is likely to have consequences in a number of disease conditions such as stroke, atherosclerosis, liver fibrosis, thrombosis and cancer. To ...
journal_title:Thrombosis research
pub_type: 杂志文章
doi:10.1016/j.thromres.2019.08.016
更新日期:2019-10-01 00:00:00