From protein total synthesis to peptide transamidation and metathesis: playing with the reversibility of N,S-acyl or N,Se-acyl migration reactions.

abstract：:Lipopolysaccharide (LPS), a glycolipid found in the outer membrane of Gram-negative bacteria, is a potent elicitor of innate immune responses in mammals. A typical LPS molecule is composed of three different structural domains: a polysaccharide called the O-antigen, a core oligosaccharide, and Lipid A. Lipid A is the ...

journal_title：Current opinion in chemical biology

authors： Xiao X,Sankaranarayanan K,Khosla C

更新日期：2017-10-01 00:00:00

abstract：:RNA editing in kinetoplastids involves post-transcriptional insertion and deletion of uridylates (Us) to produce mature mitochondrial mRNAs with sequences specified by trans acting small guide RNAs. In vitro studies indicate the reaction pathway involves endonucleolytic cleavage of the precursor mRNA at the editing si...

journal_title：Current opinion in chemical biology

authors： Stuart K,Allen TE,Kable ML,Lawson S

更新日期：1997-10-01 00:00:00

abstract：:Aside from the demonstration that individual molecular traits of enzymes can be evolutionarily optimised, the discovery that several traits can be simultaneously optimised is a major advance. The first observations of the effects of evolutionary optimisation at the structural level, through X-ray crystallography, rein...

journal_title：Current opinion in chemical biology

authors： Sutherland JD

更新日期：2000-06-01 00:00:00

abstract：:Lipids are a vital class of molecules that play important and varied roles in biological processes, however, fully understanding these roles is extremely difficult due to the immense number and diversity of possible lipid species. While recent advances in chromatography and high resolution mass spectrometry have great...

journal_title：Current opinion in chemical biology

authors： Zheng X,Smith RD,Baker ES

更新日期：2018-02-01 00:00:00

abstract：:By acoustically detecting the optical absorption contrast, photoacoustic (PA) tomography (PAT) has broken the penetration limits of traditional high-resolution optical imaging. Through spectroscopic analysis of the target's optical absorption, PAT can identify a wealth of endogenous and exogenous molecules and thus is...

journal_title：Current opinion in chemical biology

authors： Yao J,Wang LV

更新日期：2018-08-01 00:00:00

abstract：:New catalysts and reaction conditions have been developed for the dynamic kinetic resolution or deracemisation of racemic mixtures of chiral compounds. Specific functional groups that lend themselves particularly well to this approach include chiral secondary alcohols, alpha-amino acids, amines and carboxylic acids. A...

journal_title：Current opinion in chemical biology

authors： Turner NJ

更新日期：2004-04-01 00:00:00

abstract：:Over the past two decades, peptides have expanded the toolbox of additives for the preparation of noble metal nanoparticles. Their functional and structural modularity and accompanying molecular recognition and self-assembly properties offer unique opportunities for the controlled formation of NPs. Within this review,...

journal_title：Current opinion in chemical biology

authors： Corra S,Shoshan MS,Wennemers H

更新日期：2017-10-01 00:00:00

abstract：:The oceans are filled with an amazing variety of biological materials including the glues and cements of mussels, barnacles, tube worms, algae, and starfish. Recent studies on mussel adhesive are providing increasing evidence for a unique mechanism of material generation involving iron-induced protein oxidation and cr...

journal_title：Current opinion in chemical biology

authors： Wilker JJ

更新日期：2010-04-01 00:00:00

abstract：:Protein-polymer conjugates are of interest to researchers in diverse fields. Attachment of polymers to proteins results in improved pharmacokinetics, which is important in medicine. From an engineering standpoint, conjugates are exciting because they exhibit properties of both the biomolecules and synthetic polymers. ...

journal_title：Current opinion in chemical biology

authors： Grover GN,Maynard HD

更新日期：2010-12-01 00:00:00

abstract：:Study of protein adsorption to solid surfaces continues to be substantial because of its role in cellular responses to biomaterials, interest in molecular aspects such as conformation and orientation, new methods for making protein repellent surfaces, and new application areas such as nanoparticles and microfluidics. ...

journal_title：Current opinion in chemical biology

authors： Szott LM,Horbett TA

更新日期：2011-10-01 00:00:00

abstract：:A number of nitrile-related enzymes have been screened over the past year for use in synthetic applications. There have also been significant advances in our understanding of the structures and modes of regulation of metal-containing nitrile hydratases. Enzyme structural characterization has provided new insights into...

journal_title：Current opinion in chemical biology

authors： Kobayashi M,Shimizu S

更新日期：2000-02-01 00:00:00

abstract：:Synthetic biologists typically construct new pathways within existing cells. While useful, this approach in many ways ignores the undefined but necessary components of life. A growing number of laboratories have begun to try to remove some of the mysteries of cellular life by building life-like systems from non-living...

journal_title：Current opinion in chemical biology

authors： Forlin M,Lentini R,Mansy SS

更新日期：2012-12-01 00:00:00

abstract：:Systems modeling is emerging as a valuable tool in therapeutics. This is seen by the increasing use of clinically relevant computational models and a rise in systems biology companies working with the pharmaceutical industry. Systems models have helped understand the effects of pharmacological intervention at receptor...

journal_title：Current opinion in chemical biology

authors： Rajasethupathy P,Vayttaden SJ,Bhalla US

更新日期：2005-08-01 00:00:00

abstract：:The ability of cobalamin to coordinate different upper axial ligands gives rise to a diversity of reactivity. Traditionally, adenosylcobalamin is associated with radical-based rearrangements, and methylcobalamin with methyl cation transfers. Recently, however, a new role for adenosylcobalamin has been discovered as a ...

journal_title：Current opinion in chemical biology

authors： Bridwell-Rabb J,Drennan CL

更新日期：2017-04-01 00:00:00

abstract：:In many species, double-stranded RNA can specifically and effectively silence genes. This newly discovered biological phenomenon, called RNA interference (RNAi), has practical implications for functional genomics. As shown by two recent reports, RNAi provides a rapid method to test the function of genes in the nematod...

journal_title：Current opinion in chemical biology

authors： Barstead R

更新日期：2001-02-01 00:00:00

abstract：:A long-standing goal of molecular imaging is to visualize cellular function within the context of living animals, necessitating the development of reporter genes compatible with deeply penetrant imaging modalities such as ultrasound and magnetic resonance imaging (MRI). Until recently, no reporter genes for ultrasound...

journal_title：Current opinion in chemical biology

authors： Lu GJ,Farhadi A,Mukherjee A,Shapiro MG

更新日期：2018-08-01 00:00:00

abstract：:Synthetic biology offers a bottom-up engineering approach that intends to understand complex systems via design-build-test cycles. Embryonic development comprises complex processes that originate at the level of gene regulatory networks in a cell and emerge into collective cellular behaviors with multicellular forms a...

journal_title：Current opinion in chemical biology

authors： Ebrahimkhani MR,Ebisuya M

更新日期：2019-10-01 00:00:00

abstract：:There has been a significant increase in the number of compounds synthesized in early drug-discovery programs with the advances in combinatorial chemistry and high-throughput biological screening efforts. Various in silico, in vitro and in situ approaches have been described in literature that achieve higher throughpu...

journal_title：Current opinion in chemical biology

authors： Chaturvedi PR,Decker CJ,Odinecs A

更新日期：2001-08-01 00:00:00

abstract：:The chemokines are a large superfamily of chemotactic cytokines that are utilized to direct the trafficking and migration of leukocytes within the immune system. The chemokines mediate their activity through a large family of G-protein-coupled receptors, and thus are highly tractable as therapeutic targets. Exciting a...

journal_title：Current opinion in chemical biology

authors： Cascieri MA,Springer MS

更新日期：2000-08-01 00:00:00

abstract：:Protein citrullination is a post-translational modification of arginine that is catalyzed by the Protein Arginine Deiminase (PAD) family of enzymes. Aberrantly increased citrullination is associated with a host of inflammatory diseases and cancer and PAD inhibitors have shown remarkable efficacy in a range of diseases...

journal_title：Current opinion in chemical biology

authors： Clancy KW,Weerapana E,Thompson PR

更新日期：2016-02-01 00:00:00

abstract：:The site specific, co-translational introduction of unnatural amino acids into proteins produced in cells has been facilitated by the development of the pyrrolysyl-tRNA synthetase/tRNACUA pair. This pair can now be used to direct the site-specific incorporation of designer amino acids in E. coli, yeast, mammalian cell...

journal_title：Current opinion in chemical biology

authors： Elliott TS,Bianco A,Chin JW

更新日期：2014-08-01 00:00:00

abstract：:Functional amino acids that play critical roles in catalysis and regulation are known to display elevated nucleophilicity and can be selectively targeted for covalent modification by reactive electrophiles. Chemical-proteomic platforms, such as activity-based protein profiling (ABPP), exploit this reactivity by utiliz...

journal_title：Current opinion in chemical biology

authors： Shannon DA,Weerapana E

更新日期：2015-02-01 00:00:00

abstract：:There is growing interest in the design of synthetic molecules that mimic the structures and functions of epitopes found on the surface of peptides and proteins. Epitope mimetics can provide valuable tools to probe complex biological processes, as well as interesting leads for drug and vaccine discovery. One applicati...

journal_title：Current opinion in chemical biology

authors： Robinson JA

更新日期：2011-06-01 00:00:00

abstract：:Recent advances in multidimensional nuclear magnetic resonance methodology to obtain 1H, 15N and 13C resonance assignments, interproton distance and torsion angle restraints, and restraints that characterize long-range order, coupled with new methods of structure refinement and novel methods for reducing linewidths, h...

journal_title：Current opinion in chemical biology

authors： Clore GM,Gronenborn AM

更新日期：1998-10-01 00:00:00

abstract：:The photorespiratory pathway, in short photorespiration, is an essential metabolite repair pathway that allows the photosynthetic CO2 fixation of plants to occur in the presence of oxygen. It is necessary because oxygen is a competing substrate of the CO2-fixing enzyme ribulose 1,5-bisphosphate carboxylase, forming 2-...

journal_title：Current opinion in chemical biology

authors： Hagemann M,Bauwe H

更新日期：2016-12-01 00:00:00

abstract：:The reactive oxygen and nitrogen species superoxide ion, nitric oxide, nitrogen dioxide and peroxynitrite ion all react with biological target molecules. Some of these interactions are carefully orchestrated segments of signal transduction cascades or part of the armamentarium of the immune system, others are patholog...

journal_title：Current opinion in chemical biology

authors： Groves JT

更新日期：1999-04-01 00:00:00

abstract：:Modular peptide synthetases, which act as the protein templates for the synthesis of a large number of peptide antibiotics and siderophores, hold great potential for the development of novel compounds. Recently, significant progress has been made towards understanding their molecular architecture and substrate specifi...

journal_title：Current opinion in chemical biology

authors： Mootz HD,Marahiel MA

更新日期：1997-12-01 00:00:00

abstract：:Signal amplification in conventional enzyme-based biosensors is not high enough to achieve the ultrasensitive detection of biomolecules. In recent years, signal amplification has been improved by combining enzymatic reactions with redox cycling or employing multienzyme labels per detection probe. Electrochemical-chemi...

journal_title：Current opinion in chemical biology

authors： Yang H

更新日期：2012-08-01 00:00:00

abstract：:Nanoparticle-based therapeutics have received increasing attention, as these systems can alleviate many drawbacks of conventional therapy. Metal-organic frameworks (MOFs), a new class of hybrid materials composed of metal ions and organic bridging ligands, have emerged as a promising platform for drug delivery, owing ...

journal_title：Current opinion in chemical biology

authors： Huxford RC,Della Rocca J,Lin W

更新日期：2010-04-01 00:00:00

abstract：:Natural sources offer a wealth of chemically diverse compounds that have been evolutionary preselected to modulate biochemical pathways. Several industrial and academic groups are accessing this source using advanced technology platforms. Methods have been reported to generate large and diverse natural-product librari...

journal_title：Current opinion in chemical biology

authors： Abel U,Koch C,Speitling M,Hansske FG

更新日期：2002-08-01 00:00:00