Abstract：
:Protein citrullination is a post-translational modification of arginine that is catalyzed by the Protein Arginine Deiminase (PAD) family of enzymes. Aberrantly increased citrullination is associated with a host of inflammatory diseases and cancer and PAD inhibitors have shown remarkable efficacy in a range of diseases including rheumatoid arthritis, lupus, atherosclerosis, and ulcerative colitis. In rheumatoid arthritis, citrullinated proteins serve as key antigens for rheumatoid arthritis-associated autoantibodies. These data suggest that citrullinated proteins may serve more generally as biomarkers of specific disease states, however, the identification of citrullinated residues remains challenging due to the small 1Da mass change that occurs upon citrullination. Herein, we highlight the available techniques to identify citrullinated proteins/residues focusing on advanced MS techniques as well as chemical derivatization strategies that are currently being employed to identify citrullinated proteins as well as the specific residues modified within those proteins.
journal_name
Curr Opin Chem Bioljournal_title
Current opinion in chemical biologyauthors
Clancy KW,Weerapana E,Thompson PRdoi
10.1016/j.cbpa.2015.10.014subject
Has Abstractpub_date
2016-02-01 00:00:00pages
1-6eissn
1367-5931issn
1879-0402pii
S1367-5931(15)00123-4journal_volume
30pub_type
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
pub_type： 杂志文章,评审
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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journal_title：Current opinion in chemical biology
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