Abstract:
:Functional amino acids that play critical roles in catalysis and regulation are known to display elevated nucleophilicity and can be selectively targeted for covalent modification by reactive electrophiles. Chemical-proteomic platforms, such as activity-based protein profiling (ABPP), exploit this reactivity by utilizing chemical probes to covalently modify active-site residues to inform on the functional state of enzymes within complex proteomes. These and other applications rely on the availability of a diverse array of electrophiles and detailed knowledge of the reactivity and amino-acid selectivity of these groups. Here, we survey the current landscape of electrophiles that covalently target various nucleophilic amino acids in proteins and highlight proteomic applications that have benefited from the unique properties of these electrophiles.
journal_name
Curr Opin Chem Bioljournal_title
Current opinion in chemical biologyauthors
Shannon DA,Weerapana Edoi
10.1016/j.cbpa.2014.10.021subject
Has Abstractpub_date
2015-02-01 00:00:00pages
18-26eissn
1367-5931issn
1879-0402pii
S1367-5931(14)00161-6journal_volume
24pub_type
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更新日期:2011-06-01 00:00:00
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