Mechanistic insights into Diels-Alder reactions in natural product biosynthesis.

abstract：:The introduction of fluorine atoms into organic molecules has received considerable attention as these organofluorines have often found widespread applications in bioorganic chemistry, medicinal chemistry and biomaterial science. Despite innovation of synthetic C-F forming methodologies, selective fluorination is stil...

journal_title：Current opinion in chemical biology

authors： Wu L,Maglangit F,Deng H

更新日期：2020-04-01 00:00:00

abstract：:Recently, the hypothesis that protein motions are involved in enzymatic turnover has gained significant attention. We review cases where there is evidence that protein motions are rate-limiting in the overall catalytic cycle and examine experimental and theoretical evidence for how such motions enhance the probability...

journal_title：Current opinion in chemical biology

authors： Nashine VC,Hammes-Schiffer S,Benkovic SJ

更新日期：2010-10-01 00:00:00

abstract：:Immunoassays are indispensable for research and clinical analysis, and following the emergence of the omics paradigm, multiplexing of immunoassays is more needed than ever. Cross-reactivity (CR) in multiplexed immunoassays has been unexpectedly difficult to mitigate, preventing scaling up of multiplexing, limiting ass...

journal_title：Current opinion in chemical biology

authors： Juncker D,Bergeron S,Laforte V,Li H

更新日期：2014-02-01 00:00:00

abstract：:Structural modification of naturally occurring beta-lactams and beta-lactones is a highly effective strategy for generating drugs for treating bacterial infections, cancer, obesity, and hyperlipidemia. These drugs acylate catalytic amino acids (serine, threonine, or cysteine) in enzyme targets such as penicillin-bindi...

journal_title：Current opinion in chemical biology

authors： Kluge AF,Petter RC

更新日期：2010-06-01 00:00:00

abstract：:The nature of the oxidized halogen intermediate in vanadium bromoperoxidase has recently been shown to depend on the nature of the organic substrate. For example, in the presence of indoles, vanadium bromoperoxidase does not release a freely diffusible oxidized halogen intermediate (such as HOBr+/-BR2+/-Br3-). Regiose...

journal_title：Current opinion in chemical biology

authors： Butler A

更新日期：1998-04-01 00:00:00

abstract：:Molecular oxygen plays an indispensable role as a terminal electron acceptor in the electron transport chain in mitochondria. Acute or chronic oxygen deprivation (hypoxia) in organisms results in various diseases, and the elucidation of the pathogenic mechanism of hypoxia-related diseases and various cellular response...

journal_title：Current opinion in chemical biology

authors： Tobita S,Yoshihara T

更新日期：2016-08-01 00:00:00

abstract：:Structural genomics (SG) has significantly increased the number of novel protein structures of targets with medical relevance. In the protein kinase area, SG has contributed >50% of all novel kinases structures during the past three years and determined more than 30 novel catalytic domain structures. Many of the relea...

journal_title：Current opinion in chemical biology

authors： Marsden BD,Knapp S

更新日期：2008-02-01 00:00:00

abstract：:The industrial application of enzymes that can withstand harsh conditions has greatly increased over the past decade. This is mainly a result of the discovery of novel enzymes from extremophilic microorganisms. Recent advances in the study of extremozymes point to the acceleration of this trend. In particular, enzymes...

journal_title：Current opinion in chemical biology

authors： Demirjian DC,Morís-Varas F,Cassidy CS

更新日期：2001-04-01 00:00:00

abstract：:Study of protein adsorption to solid surfaces continues to be substantial because of its role in cellular responses to biomaterials, interest in molecular aspects such as conformation and orientation, new methods for making protein repellent surfaces, and new application areas such as nanoparticles and microfluidics. ...

journal_title：Current opinion in chemical biology

authors： Szott LM,Horbett TA

更新日期：2011-10-01 00:00:00

abstract：:The application of molecular cloning has revealed the phenomenal diversity and complexity of the phosphodiesterase isoenzyme family. Thus, more than 30 human phosphodiesterases are now known; all are apparently necessary for the seemingly simple task of hydrolysing the 3'-ester bond of either cyclic adenosine monophos...

journal_title：Current opinion in chemical biology

authors： Perry MJ,Higgs GA

更新日期：1998-08-01 00:00:00

abstract：:Peptides that recognize specific cell types promise to be valuable tools both in research and clinical applications. Cell-specific peptides can be useful as drug delivery vehicles, diagnostic agents, affinity reagents for cell purification, gene therapy delivery agents, and research tools to probe the nature of a cell...

journal_title：Current opinion in chemical biology

authors： Brown KC

更新日期：2000-02-01 00:00:00

abstract：:The design of dimeric coiled-coils has ultimately led to novel applications, such as self-replicating peptide systems, whereas the structural features of the less common trimeric coiled-coil continue to be elucidated. Novel topologies have been discovered in designed proteins, as exemplified by the right-handed tetram...

journal_title：Current opinion in chemical biology

authors： Micklatcher C,Chmielewski J

更新日期：1999-12-01 00:00:00

abstract：:Mass spectrometry has become a choice method for broad-spectrum metabolite analysis in both fundamental and applied research. This can range from comprehensive analysis achieved through time-consuming chromatography to the rapid analysis of a few target metabolites without chromatography. In this review article, we hi...

journal_title：Current opinion in chemical biology

authors： de Raad M,Fischer CR,Northen TR

更新日期：2016-02-01 00:00:00

abstract：:Natural evolution has produced a great diversity of proteins that can be harnessed for numerous applications in biotechnology and pharmaceutical science. Commonly, specific applications require proteins to be tailored by protein engineering. Directed evolution is a type of protein engineering that yields proteins with...

journal_title：Current opinion in chemical biology

authors： Lane MD,Seelig B

更新日期：2014-10-01 00:00:00

abstract：:Over the past two decades, there has been intense interest in designing and implementing selective (bioorthogonal) reactions for biomolecule tracking. Here we review the most widely used bioorthogonal chemistries in live cells and animals, drawing particular attention to the unique functional groups underlying these t...

journal_title：Current opinion in chemical biology

authors： Shih HW,Kamber DN,Prescher JA

更新日期：2014-08-01 00:00:00

abstract：:Small molecule production in microbial hosts is limited by the accumulation of the product inside the cell. Efflux transporters show promise as a solution to removal of the often-toxic products. Recent advances in transporter identification through expression profiling, heterologous expression, and knockout studies ha...

journal_title：Current opinion in chemical biology

authors： Boyarskiy S,Tullman-Ercek D

更新日期：2015-10-01 00:00:00

abstract：:The first photocaged complexes appeared in the mid-1980s specifically to complement the ongoing work studying intracellular Ca(2+) signaling with fluorescent probes. Recently, the field of biological inorganic chemistry has focused increasingly on the homeostasis mechanisms and possible signaling functions of transiti...

journal_title：Current opinion in chemical biology

authors： Gwizdala C,Burdette SC

更新日期：2013-04-01 00:00:00

abstract：:Metabolomics and lipidomics aim to comprehensively measure the dynamic changes of all metabolites and lipids that are present in biological systems. The use of ion mobility-mass spectrometry (IM-MS) for metabolomics and lipidomics has facilitated the separation and the identification of metabolites and lipids in compl...

journal_title：Current opinion in chemical biology

authors： Zhou Z,Tu J,Zhu ZJ

更新日期：2018-02-01 00:00:00

abstract：:In recent years, an increasing number of proteins have been discovered which utilize heme cofactors to sense oxygen, carbon monoxide and nitric oxide. The identification and characterization of these proteins are revising our understanding of heme-mediated allostery first established in the early 1960s. Biochemical an...

journal_title：Current opinion in chemical biology

authors： Chan MK

更新日期：2001-04-01 00:00:00

abstract：:Modular peptide synthetases, which act as the protein templates for the synthesis of a large number of peptide antibiotics and siderophores, hold great potential for the development of novel compounds. Recently, significant progress has been made towards understanding their molecular architecture and substrate specifi...

journal_title：Current opinion in chemical biology

authors： Mootz HD,Marahiel MA

更新日期：1997-12-01 00:00:00

abstract：:The chemokines are a large superfamily of chemotactic cytokines that are utilized to direct the trafficking and migration of leukocytes within the immune system. The chemokines mediate their activity through a large family of G-protein-coupled receptors, and thus are highly tractable as therapeutic targets. Exciting a...

journal_title：Current opinion in chemical biology

authors： Cascieri MA,Springer MS

更新日期：2000-08-01 00:00:00

abstract：:The cytochrome bc(1) complex is a key mitochondrial enzyme that catalyses transfer of electrons maintaining the membrane potential of mitochondria. Currently, atovaquone is the only drug in clinical use targeting the Plasmodium falciparum bc(1) complex. The rapid emergence of resistance to atovaquone resulted in a cos...

journal_title：Current opinion in chemical biology

authors： Barton V,Fisher N,Biagini GA,Ward SA,O'Neill PM

更新日期：2010-08-01 00:00:00

abstract：:Recent structural information suggests that the HC(X)5R active-site motif defines three distinct evolutionary families of phosphatases that employ a common catalytic mechanism. In two instances, regulation of phosphatase activity employs autoinhibitory mechanisms involving either intermolecular or intramolecular inter...

journal_title：Current opinion in chemical biology

authors： Denu JM,Dixon JE

更新日期：1998-10-01 00:00:00

abstract：:Sugar nucleotide-modifying enzymes of the short-chain dehydrogenase/reductase type use transient oxidation-reduction by a tightly bound nicotinamide cofactor as a common strategy of catalysis to promote a diverse set of reactions, including decarboxylation, single- or double-site epimerization, and dehydration. Althou...

journal_title：Current opinion in chemical biology

authors： Borg AJE,Beerens K,Pfeiffer M,Desmet T,Nidetzky B

更新日期：2020-11-06 00:00:00

abstract：:Lysine acetylation has emerged as a key post-translational modification found at many sites throughout the cell. It plays an important role in epigenetic processes, and more generally in the regulation of protein stability and interactions. Acetyl groups are installed by lysine acetyltransferases and removed by lysine...

journal_title：Current opinion in chemical biology

authors： Schiedel M,Conway SJ

更新日期：2018-08-01 00:00:00

abstract：:Amide forming reactions are central to the field of peptide and protein synthesis and are considered to be poorly reversible reactions owing to the high stability of peptide bonds. One amide-forming reaction is native chemical ligation (NCL) which is driven by a sulfur to nitrogen acyl migration process from a transie...

journal_title：Current opinion in chemical biology

authors： Melnyk O,Agouridas V

更新日期：2014-10-01 00:00:00

abstract：:The function of nucleic acids has been an endless source of discovery and invention that has drastically enhanced our appreciation of DNA and RNA as multifaceted polymers. It is now widely known that nucleic acids can act as enzymes (deoxyribozymes and ribozymes) and as receptors (aptamers), and that these functional ...

journal_title：Current opinion in chemical biology

authors： Navani NK,Li Y

更新日期：2006-06-01 00:00:00

abstract：:Systems modeling is emerging as a valuable tool in therapeutics. This is seen by the increasing use of clinically relevant computational models and a rise in systems biology companies working with the pharmaceutical industry. Systems models have helped understand the effects of pharmacological intervention at receptor...

journal_title：Current opinion in chemical biology

authors： Rajasethupathy P,Vayttaden SJ,Bhalla US

更新日期：2005-08-01 00:00:00

abstract：:Efficient library design is an ongoing challenge for investigators seeking novel ligands for proteins, whether for drug discovery or chemical biology. Strategies that add neglected chemistry or exclude unproductive compounds are two dominant recent themes, as is a growing awareness of molecular complexity and its impl...

journal_title：Current opinion in chemical biology

authors： Irwin JJ

更新日期：2006-08-01 00:00:00

abstract：:New insights have been gained into the therapeutic relevance of the sphingosine 1-phosphate (S1P) pathway, on the basis of reverse pharmacological approaches to defining the mechanism of action of the immunosuppressive agent FTY720. Natural and synthetic sphingosine 1-phosphate receptor agonists can make picomolar int...

journal_title：Current opinion in chemical biology

authors： Rosen H,Liao J

更新日期：2003-08-01 00:00:00