Abstract:
:Over the past two decades, there has been intense interest in designing and implementing selective (bioorthogonal) reactions for biomolecule tracking. Here we review the most widely used bioorthogonal chemistries in live cells and animals, drawing particular attention to the unique functional groups underlying these transformations. We also describe recent efforts to tune functional group reactivities and stabilities to access even more rapid and selective chemistries. Last, we highlight ongoing challenges in identifying new bioorthogonal reagents and combinations of reactions that can be used concurrently to tag multiple biomolecules.
journal_name
Curr Opin Chem Bioljournal_title
Current opinion in chemical biologyauthors
Shih HW,Kamber DN,Prescher JAdoi
10.1016/j.cbpa.2014.07.002subject
Has Abstractpub_date
2014-08-01 00:00:00pages
103-11eissn
1367-5931issn
1879-0402pii
S1367-5931(14)00089-1journal_volume
21pub_type
杂志文章,评审abstract::Modular peptide synthetases, which act as the protein templates for the synthesis of a large number of peptide antibiotics and siderophores, hold great potential for the development of novel compounds. Recently, significant progress has been made towards understanding their molecular architecture and substrate specifi...
journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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abstract::Electron bifurcation, or the coupling of exergonic and endergonic oxidation-reduction reactions, was discovered by Peter Mitchell and provides an elegant mechanism to rationalize and understand the logic that underpins the Q cycle of the respiratory chain. Thought to be a unique reaction of respiratory complex III for...
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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