Enzyme catalysed deracemisation and dynamic kinetic resolution reactions.

abstract：:Major advances have been made in our understanding of cytochrome c oxidase owing to continued crystallographic work on important intermediates. This, together with a wealth of data derived from selective mutations and sophisticated spectroscopic probes, has provided significant new insights into oxidase dioxygen chemi...

journal_title：Current opinion in chemical biology

authors： Poulos TL,Li H,Raman CS

更新日期：1999-04-01 00:00:00

abstract：:In recent years, an increasing number of proteins have been discovered which utilize heme cofactors to sense oxygen, carbon monoxide and nitric oxide. The identification and characterization of these proteins are revising our understanding of heme-mediated allostery first established in the early 1960s. Biochemical an...

journal_title：Current opinion in chemical biology

authors： Chan MK

更新日期：2001-04-01 00:00:00

abstract：:Lipids are a vital class of molecules that play important and varied roles in biological processes, however, fully understanding these roles is extremely difficult due to the immense number and diversity of possible lipid species. While recent advances in chromatography and high resolution mass spectrometry have great...

journal_title：Current opinion in chemical biology

authors： Zheng X,Smith RD,Baker ES

更新日期：2018-02-01 00:00:00

abstract：:The translation of nucleic acids into synthetic structures with expanded functional potential has been the subject of considerable research, with applications including small-molecule and polymer evolution, reaction discovery and sensing. Here, we review properties of nucleic-acid-templated synthesis in the context of...

journal_title：Current opinion in chemical biology

authors： Calderone CT,Liu DR

更新日期：2004-12-01 00:00:00

abstract：:The notorious difficulty of elucidating structures of membrane transporters by crystallography has long prevented our understanding of active transport mechanism coupled with ion/proton transport. The determination of the first crystal structure of the drug/H+ antiporter AcrB was a breakthrough for structure-based und...

journal_title：Current opinion in chemical biology

authors： Tamura N,Konishi S,Yamaguchi A

更新日期：2003-10-01 00:00:00

abstract：:Recent structural information suggests that the HC(X)5R active-site motif defines three distinct evolutionary families of phosphatases that employ a common catalytic mechanism. In two instances, regulation of phosphatase activity employs autoinhibitory mechanisms involving either intermolecular or intramolecular inter...

journal_title：Current opinion in chemical biology

authors： Denu JM,Dixon JE

更新日期：1998-10-01 00:00:00

abstract：:Systems modeling is emerging as a valuable tool in therapeutics. This is seen by the increasing use of clinically relevant computational models and a rise in systems biology companies working with the pharmaceutical industry. Systems models have helped understand the effects of pharmacological intervention at receptor...

journal_title：Current opinion in chemical biology

authors： Rajasethupathy P,Vayttaden SJ,Bhalla US

更新日期：2005-08-01 00:00:00

abstract：:The eukaryotic ribonucleoprotein reverse transcriptase (RT) telomerase uses a template within its integral RNA subunit to extend chromosome ends by synthesis of single-stranded telomeric repeats. Telomerase is adapted to its unique cellular role by an ability to release product DNA in single-stranded form, regeneratin...

journal_title：Current opinion in chemical biology

authors： Collins K

更新日期：2011-10-01 00:00:00

abstract：:Molecular oxygen plays an indispensable role as a terminal electron acceptor in the electron transport chain in mitochondria. Acute or chronic oxygen deprivation (hypoxia) in organisms results in various diseases, and the elucidation of the pathogenic mechanism of hypoxia-related diseases and various cellular response...

journal_title：Current opinion in chemical biology

authors： Tobita S,Yoshihara T

更新日期：2016-08-01 00:00:00

abstract：:Mass spectrometry has become a choice method for broad-spectrum metabolite analysis in both fundamental and applied research. This can range from comprehensive analysis achieved through time-consuming chromatography to the rapid analysis of a few target metabolites without chromatography. In this review article, we hi...

journal_title：Current opinion in chemical biology

authors： de Raad M,Fischer CR,Northen TR

更新日期：2016-02-01 00:00:00

abstract：:Amide forming reactions are central to the field of peptide and protein synthesis and are considered to be poorly reversible reactions owing to the high stability of peptide bonds. One amide-forming reaction is native chemical ligation (NCL) which is driven by a sulfur to nitrogen acyl migration process from a transie...

journal_title：Current opinion in chemical biology

authors： Melnyk O,Agouridas V

更新日期：2014-10-01 00:00:00

abstract：:Natural sources offer a wealth of chemically diverse compounds that have been evolutionary preselected to modulate biochemical pathways. Several industrial and academic groups are accessing this source using advanced technology platforms. Methods have been reported to generate large and diverse natural-product librari...

journal_title：Current opinion in chemical biology

authors： Abel U,Koch C,Speitling M,Hansske FG

更新日期：2002-08-01 00:00:00

abstract：:The definition of molecular diversity and the development of measures for assessing the similarity or dissimilarity of molecules are central tasks for the design of novel biologically active compounds. Combinatorial chemistry allows the coupling of mathematical optimisation methods that do not require the a priori kno...

journal_title：Current opinion in chemical biology

authors： Weber L

更新日期：1998-06-01 00:00:00

abstract：:Nanoparticle-based therapeutics have received increasing attention, as these systems can alleviate many drawbacks of conventional therapy. Metal-organic frameworks (MOFs), a new class of hybrid materials composed of metal ions and organic bridging ligands, have emerged as a promising platform for drug delivery, owing ...

journal_title：Current opinion in chemical biology

authors： Huxford RC,Della Rocca J,Lin W

更新日期：2010-04-01 00:00:00

abstract：:Oxidative cysteine modifications have emerged as a central mechanism for dynamic post-translational regulation of all major protein classes and correlate with many disease states. Elucidating the precise roles of cysteine oxidation in physiology and pathology presents a major challenge. This article reviews the curren...

journal_title：Current opinion in chemical biology

authors： Leonard SE,Carroll KS

更新日期：2011-02-01 00:00:00

abstract：:The (re)design of enzymes to catalyze 'new' reactions is a topic of considerable practical and intellectual interest. Directed evolution (random mutagenesis followed by screening/selection) has been used widely to identify novel biocatalysts. However, 'rational' approaches using either natural divergent evolution or c...

journal_title：Current opinion in chemical biology

authors： Gerlt JA,Babbitt PC

更新日期：2009-02-01 00:00:00

abstract：:Lysine acetylation has emerged as a key post-translational modification found at many sites throughout the cell. It plays an important role in epigenetic processes, and more generally in the regulation of protein stability and interactions. Acetyl groups are installed by lysine acetyltransferases and removed by lysine...

journal_title：Current opinion in chemical biology

authors： Schiedel M,Conway SJ

更新日期：2018-08-01 00:00:00

abstract：:Chromatin, the nucleoprotein complex organizing the genome, is central in regulating gene expression and genome organization. Chromatin conformational dynamics, controlled by histone post-translational modifications (PTM) and effector proteins, play a key role in this regulatory function. Recent developments in chemic...

journal_title：Current opinion in chemical biology

authors： Boichenko I,Fierz B

更新日期：2019-08-01 00:00:00

abstract：:Exacerbation of climate change and air pollution around the world have emphasized the necessity of replacing fossil fuels with clean and sustainable energy. Metabolic engineering has provided strategies to engineer diverse organisms for the production of biofuels from renewable carbon sources. Although some of the pro...

journal_title：Current opinion in chemical biology

authors： Choi KR,Jiao S,Lee SY

更新日期：2020-12-01 00:00:00

abstract：:Oligosaccharide recognition is a major means of bacterial-host cell attachment. Bacterial-host receptor binding can subvert host signaling pathways to cause pathology. In addition, pathogenic bacteria can utilize more than one recognition system to bind host cells. Recent studies of Helicobacter pylori illustrate both...

journal_title：Current opinion in chemical biology

authors： Lingwood CA

更新日期：1998-12-01 00:00:00

abstract：:A broad range of biochemicals, from proteins to nucleic acids, function properly only when associated with a metal, usually a divalent cation. Not any divalent metal will do: these metals differ in their ionic radius, dissociation in water, ionization potential, and number of unpaired electrons in their outer shells, ...

journal_title：Current opinion in chemical biology

authors： Bloom AJ

更新日期：2019-04-01 00:00:00

abstract：:The field of computational protein design is reaching its adolescence. Protein design algorithms have been applied to design or engineer proteins that fold, fold faster, catalyze, catalyze faster, signal, and adopt preferred conformational states. Further developments of scoring functions, sampling strategies, and opt...

journal_title：Current opinion in chemical biology

authors： Kraemer-Pecore CM,Wollacott AM,Desjarlais JR

更新日期：2001-12-01 00:00:00

abstract：:Synthetic biologists typically construct new pathways within existing cells. While useful, this approach in many ways ignores the undefined but necessary components of life. A growing number of laboratories have begun to try to remove some of the mysteries of cellular life by building life-like systems from non-living...

journal_title：Current opinion in chemical biology

authors： Forlin M,Lentini R,Mansy SS

更新日期：2012-12-01 00:00:00

abstract：:Recent studies of moonlighting functions and catalytic promiscuity provide insights into the structural and mechanistic bases of these phenomena. Moonlighting proteins that are highlighted include gephyrin, the Neurospora crassa tyrosyl tRNA synthetase, phosphoglucose isomerase, and cytochrome c. New insights into cat...

journal_title：Current opinion in chemical biology

authors： Copley SD

更新日期：2003-04-01 00:00:00

abstract：:Altered metabolite levels can drive epigenetic changes critical to development and disease. However, in many cases the specific protein-metabolite interactions that underlie this process remain enigmatic. In this review, we make the case that this fundamental missing information may be discovered by applying the tools...

journal_title：Current opinion in chemical biology

authors： Kulkarni RA,Montgomery DC,Meier JL

更新日期：2019-08-01 00:00:00

abstract：:The catalytic pathways of soluble methane monooxygenase (sMMO) and cytochrome P450CAM, iron-containing enzymes, are described and compared. Recent extensive density functional ab initio electronic structure calculations have revealed many similarities in a number of the key catalytic steps, as well as some important d...

journal_title：Current opinion in chemical biology

authors： Guallar V,Gherman BF,Lippard SJ,Friesner RA

更新日期：2002-04-01 00:00:00

abstract：:Over the past two decades, there has been intense interest in designing and implementing selective (bioorthogonal) reactions for biomolecule tracking. Here we review the most widely used bioorthogonal chemistries in live cells and animals, drawing particular attention to the unique functional groups underlying these t...

journal_title：Current opinion in chemical biology

authors： Shih HW,Kamber DN,Prescher JA

更新日期：2014-08-01 00:00:00

abstract：:Natural products containing a gamma-butyrolactone ring are abundant in nature; however, few general synthetic approaches to their stereoselective synthesis with broad structural variety are known. In this article, recent developments towards mono- and polycyclic gamma-butyrolactone natural products and analogs are dis...

journal_title：Current opinion in chemical biology

authors： Seitz M,Reiser O

更新日期：2005-06-01 00:00:00

abstract：:Fluorescence and reflectance spectroscopy provide the ability to assess tissue structure and metabolism in vivo in real time, providing improved diagnosis of pre-cancerous lesions. Reflectance spectroscopy can probe changes in epithelial nuclei that are important in pre-cancer detection, such as mean nuclear diameter,...

journal_title：Current opinion in chemical biology

authors： Sokolov K,Follen M,Richards-Kortum R

更新日期：2002-10-01 00:00:00

abstract：:Study of protein adsorption to solid surfaces continues to be substantial because of its role in cellular responses to biomaterials, interest in molecular aspects such as conformation and orientation, new methods for making protein repellent surfaces, and new application areas such as nanoparticles and microfluidics. ...

journal_title：Current opinion in chemical biology

authors： Szott LM,Horbett TA

更新日期：2011-10-01 00:00:00