Abstract:
:The notorious difficulty of elucidating structures of membrane transporters by crystallography has long prevented our understanding of active transport mechanism coupled with ion/proton transport. The determination of the first crystal structure of the drug/H+ antiporter AcrB was a breakthrough for structure-based understanding of drug/H+ antiport. However, although AcrB is a major multidrug exporter in Gram-negative organisms, the majority of bacterial drug exporters are major facilitator superfamily (MFS) drug transporters. As no crystal structures have been solved for MFS transporters, the alternative protein-engineering methods are still very useful for estimating structures and functions of drug/H+ antiporters. This review describes this alternative approach for investigating the structure and function of tetracycline/H+ antiporters.
journal_name
Curr Opin Chem Bioljournal_title
Current opinion in chemical biologyauthors
Tamura N,Konishi S,Yamaguchi Adoi
10.1016/j.cbpa.2003.08.014subject
Has Abstractpub_date
2003-10-01 00:00:00pages
570-9issue
5eissn
1367-5931issn
1879-0402pii
S1367593103001170journal_volume
7pub_type
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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abstract::Fifty years after the publication of the DNA double helix model by Watson and Crick, new nucleic acid structures keep emerging at an ever-increasing rate. The past three years have brought a flurry of new oligonucleotide structures, including those of a Hoogsteen-paired DNA duplex, Holliday junctions, DNA-drug complex...
journal_title:Current opinion in chemical biology
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abstract::Natural evolution has produced a great diversity of proteins that can be harnessed for numerous applications in biotechnology and pharmaceutical science. Commonly, specific applications require proteins to be tailored by protein engineering. Directed evolution is a type of protein engineering that yields proteins with...
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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