Abstract:
:The field of enzymology has long used small-molecule mass spectrometry. However, the direct interrogation of covalent and non-covalent intermediates by large-molecule mass spectrometry of enzymes or large peptide substrates is illuminating an increasingly diverse array of chemistries used in nature. Recent advances now allow improved detection of several modifications formed at sub-stoichiometric levels on the same polypeptide, and elucidation of intermediate dynamics with low millisecond temporal resolution. Highlighting recent applications in both ribosomal and non-ribosomal biosynthesis of natural products, along with acetyl transferases, sulfonucleotide reducatases, and PEP-utilizing enzymes, the utility of small- and large-molecule mass spectrometry to reveal enzyme intermediates and illuminate mechanism is described briefly. From ever more complex mixtures, mass spectrometry continues to evolve into a key technology for a larger number of today's enzymologists.
journal_name
Curr Opin Chem Bioljournal_title
Current opinion in chemical biologyauthors
Kelleher NL,Hicks LMdoi
10.1016/j.cbpa.2005.08.018subject
Has Abstractpub_date
2005-10-01 00:00:00pages
424-30issue
5eissn
1367-5931issn
1879-0402pii
S1367-5931(05)00120-1journal_volume
9pub_type
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