Abstract:
:Synthetic biologists typically construct new pathways within existing cells. While useful, this approach in many ways ignores the undefined but necessary components of life. A growing number of laboratories have begun to try to remove some of the mysteries of cellular life by building life-like systems from non-living component parts. Some of these attempts rely on purely chemical and physical forces alone without the aid of biological molecules, while others try to build artificial cells from the parts of life, such as nucleic acids, proteins, and lipids. Both bottom-up strategies suffer from the complication of trying to build something that remains undefined. The result has been the development of research programs that try to build systems that mimic in some way recognized living systems. Since it is difficult to quantify the mimicry of life, success often times is evaluated with a degree of subjectivity. Herein we highlight recent advances in mimicking the organization and behavior of cellular life from the bottom-up.
journal_name
Curr Opin Chem Bioljournal_title
Current opinion in chemical biologyauthors
Forlin M,Lentini R,Mansy SSdoi
10.1016/j.cbpa.2012.10.020subject
Has Abstractpub_date
2012-12-01 00:00:00pages
586-92issue
5-6eissn
1367-5931issn
1879-0402pii
S1367-5931(12)00106-8journal_volume
16pub_type
杂志文章,评审abstract::The design of dimeric coiled-coils has ultimately led to novel applications, such as self-replicating peptide systems, whereas the structural features of the less common trimeric coiled-coil continue to be elucidated. Novel topologies have been discovered in designed proteins, as exemplified by the right-handed tetram...
journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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更新日期:1998-08-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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更新日期:1999-04-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/s1367-5931(00)00204-0
更新日期:2001-06-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2013.11.012
更新日期:2014-02-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2009.06.019
更新日期:2009-10-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/s1367-5931(00)00087-9
更新日期:2000-06-01 00:00:00
abstract::The translation of nucleic acids into synthetic structures with expanded functional potential has been the subject of considerable research, with applications including small-molecule and polymer evolution, reaction discovery and sensing. Here, we review properties of nucleic-acid-templated synthesis in the context of...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2004.09.003
更新日期:2004-12-01 00:00:00
abstract::The asymmetric bioreduction of alkenes bearing an electron-withdrawing group using flavin-dependent enzymes from the 'old yellow enzyme' family at the expense of NAD(P)H yields the corresponding non-racemic alkanes going in hand with the creation of up to two chiral carbon centres. To avoid external cofactor recycling...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2007.02.025
更新日期:2007-04-01 00:00:00
abstract::Electron bifurcation, or the coupling of exergonic and endergonic oxidation-reduction reactions, was discovered by Peter Mitchell and provides an elegant mechanism to rationalize and understand the logic that underpins the Q cycle of the respiratory chain. Thought to be a unique reaction of respiratory complex III for...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2018.07.026
更新日期:2018-12-01 00:00:00
abstract::Iron is an essential nutrient for life, and its capacity to cycle between different oxidation states is required for processes spanning oxygen transport and respiration to nucleotide synthesis and epigenetic regulation. However, this same redox ability also makes iron, if not regulated properly, a potentially dangerou...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2017.12.010
更新日期:2018-04-01 00:00:00
abstract::Two recent advances have enabled the development of clinically useful, injectable, sustained-release protein formulations. The first is a nonaqueous, cryogenic atomization process that encapsulates the protein into microspheres composed of a biodegradable polymer from which the protein is released slowly. The second c...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/s1367-5931(98)80133-6
更新日期:1998-08-01 00:00:00
abstract::Rapid progress in genomics and proteomics has provided a wealth of new targets for the pharmaceutical industry, even as many older targets still remain challenging for small-molecule drug discovery. Fragment-based lead discovery, in which leads are built progressively by expanding or combining small fragments, is a ra...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2004.06.010
更新日期:2004-08-01 00:00:00
abstract::Different strategies for the creation of optical sensors with metal-dye complexes are discussed. The focus is on sensors, which are used in conjunction with pattern recognition protocols. It is shown that remarkably powerful sensors can be obtained by combining commercially available dyes with simple transition metal ...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2010.07.005
更新日期:2010-12-01 00:00:00
abstract::Single nucleotide polymorphism (SNP) genotyping has become a key technology for genetic studies. In recent years, matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry has emerged as a very powerful method for SNP genotyping. Here, we discuss our experience in implementing a high-through...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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更新日期:2002-02-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2006.04.009
更新日期:2006-06-01 00:00:00
abstract::Sugar nucleotide-modifying enzymes of the short-chain dehydrogenase/reductase type use transient oxidation-reduction by a tightly bound nicotinamide cofactor as a common strategy of catalysis to promote a diverse set of reactions, including decarboxylation, single- or double-site epimerization, and dehydration. Althou...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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更新日期:2020-11-06 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2005.08.012
更新日期:2005-10-01 00:00:00
abstract::Numerous developments in useful linkers are closely related with the advances in solid-phase organic synthesis. These linkers allow facile attachment functionalization and release molecules of interest. Designing a new anchoring group can be essential for synthesis success, especially for small molecules on solid supp...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2004.04.004
更新日期:2004-06-01 00:00:00
abstract::During the past year, remarkable progress has been made in understanding how periplasmic chaperones fold and protect protein modules that are destined for assembly into adhesive pili in Gram-negative bacteria. The first two three-dimensional structures of complexes of periplasmic chaperones with substrate pilus subuni...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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更新日期:2000-12-01 00:00:00
abstract::Recent advances in -omic profiling technologies have ushered in an era where we no longer want to merely measure the presence or absence of a biomolecule of interest, but instead hope to understand its function and interactions within larger signaling networks. Here, we review several emerging proteomic technologies c...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2020.02.001
更新日期:2020-02-01 00:00:00
abstract::New insights have been gained into the formation of the di-iron tyrosyl radical cofactor, which is essential for nucleotide reduction in the class I ribonucleotide reductases. Recent advances include Insight into the function of the tyrosyl radical in initiation of nucleotide reduction. ...
journal_title:Current opinion in chemical biology
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更新日期:2003-04-01 00:00:00
abstract::Nitrogenase catalyzes the important reactions of N2-reduction, CO-reduction and CO2-reduction at its active cofactor site. Designated the M-cluster, this complex metallocofactor is assembled through the generation of a characteristic 8Fe-core before the insertion of Mo and homocitrate that completes the stoichiometry ...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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更新日期:2016-04-01 00:00:00
abstract::Chromatin, the nucleoprotein complex organizing the genome, is central in regulating gene expression and genome organization. Chromatin conformational dynamics, controlled by histone post-translational modifications (PTM) and effector proteins, play a key role in this regulatory function. Recent developments in chemic...
journal_title:Current opinion in chemical biology
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更新日期:2019-08-01 00:00:00
abstract::Natural enzymes that catalyze Diels-Alder reactions have long been sought after, yet few enzymes have been experimentally confirmed to perform this reaction. In the past five years, several stand-alone enzymes that can catalyze the Diels-Alder reaction had been identified and characterized. Among which, the crystal st...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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更新日期:2016-12-01 00:00:00
abstract::Peptide natural products (PNPs) represent a unique class of compounds known for their fascinating structural motifs with important biological activities. Lately, PNPs have garnered a lot of interest for their application in drug discovery. Nevertheless, lack of diversity oriented synthetic/biosynthetic platforms to ge...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2016.06.006
更新日期:2016-10-01 00:00:00