Cross-reactivity in antibody microarrays and multiplexed sandwich assays: shedding light on the dark side of multiplexing.

abstract：:Study of protein adsorption to solid surfaces continues to be substantial because of its role in cellular responses to biomaterials, interest in molecular aspects such as conformation and orientation, new methods for making protein repellent surfaces, and new application areas such as nanoparticles and microfluidics. ...

journal_title：Current opinion in chemical biology

authors： Szott LM,Horbett TA

更新日期：2011-10-01 00:00:00

abstract：:There is growing interest in the design of synthetic molecules that mimic the structures and functions of epitopes found on the surface of peptides and proteins. Epitope mimetics can provide valuable tools to probe complex biological processes, as well as interesting leads for drug and vaccine discovery. One applicati...

journal_title：Current opinion in chemical biology

authors： Robinson JA

更新日期：2011-06-01 00:00:00

abstract：:Peptide nucleic acid (PNA)-encoded chemical libraries along with DNA-encoded libraries have provided a powerful new paradigm for library synthesis and ligand discovery. PNA-encoding stands out for its compatibility with standard solid phase synthesis and the technology has been used to prepare libraries of peptides, h...

journal_title：Current opinion in chemical biology

authors： Zambaldo C,Barluenga S,Winssinger N

更新日期：2015-06-01 00:00:00

abstract：:The broad utility of protein bioconjugates has created a need for new and diverse strategies for site-selective protein modification. In particular, chemical reactions that target alternative amino acid side chains or unnatural functional groups are emerging as a valuable complement to more commonly used lysine- and c...

journal_title：Current opinion in chemical biology

authors： Antos JM,Francis MB

更新日期：2006-06-01 00:00:00

abstract：:The range and diversity of solid-phase synthetic methodology continues to develop very rapidly, allowing the generation of previously inaccessible compound libraries. The design of such libraries requires consideration of linkage strategies, newly developed solid-phase reactions and emerging solution-phase processes. ...

journal_title：Current opinion in chemical biology

authors： Oliver SF,Abell C

更新日期：1999-06-01 00:00:00

abstract：:In recent years pharmaceutical companies have utilized structure-based drug design and combinatorial library design techniques to speed up their drug discovery efforts. Both approaches are routinely used in the lead discovery and lead optimization stages of the drug discovery process. Fragment-based drug design, a new...

journal_title：Current opinion in chemical biology

authors： Zhou JZ

更新日期：2008-06-01 00:00:00

abstract：:In recent years, ogliopeptides have enjoyed ever increasing interest in two areas: first, approaches to biomimetic enzyme-like activity; and second, as metal-free catalysts for enantioselective transformations of synthetic interest. The discovery and optimization of peptides for these purposes has often used the metho...

journal_title：Current opinion in chemical biology

authors： Berkessel A

更新日期：2003-06-01 00:00:00

abstract：:The application of molecular cloning has revealed the phenomenal diversity and complexity of the phosphodiesterase isoenzyme family. Thus, more than 30 human phosphodiesterases are now known; all are apparently necessary for the seemingly simple task of hydrolysing the 3'-ester bond of either cyclic adenosine monophos...

journal_title：Current opinion in chemical biology

authors： Perry MJ,Higgs GA

更新日期：1998-08-01 00:00:00

abstract：:Numerous tools for Zn2+ sensing in living cells have become available in the past three years. Among them, fluorescence imaging using fluorescent sensor molecules has been the most popular approach. Some of these sensor molecules can be used to visualize Zn2+ in living cells. Some of the biological functions of Zn2+ h...

journal_title：Current opinion in chemical biology

authors： Kikuchi K,Komatsu K,Nagano T

更新日期：2004-04-01 00:00:00

abstract：:A wide range of experiments have emerged recently regarding charge transport through DNA, including spectroscopic studies of rates of DNA-mediated electron transfer and biochemical studies of DNA base oxidation over long distances. These experiments have, in turn, led to new proposals about the way in which charge mov...

journal_title：Current opinion in chemical biology

authors： Núñez ME,Barton JK

更新日期：2000-04-01 00:00:00

abstract：:Rapid progress in genomics and proteomics has provided a wealth of new targets for the pharmaceutical industry, even as many older targets still remain challenging for small-molecule drug discovery. Fragment-based lead discovery, in which leads are built progressively by expanding or combining small fragments, is a ra...

journal_title：Current opinion in chemical biology

authors： Erlanson DA,Hansen SK

更新日期：2004-08-01 00:00:00

abstract：:Structural modification of naturally occurring beta-lactams and beta-lactones is a highly effective strategy for generating drugs for treating bacterial infections, cancer, obesity, and hyperlipidemia. These drugs acylate catalytic amino acids (serine, threonine, or cysteine) in enzyme targets such as penicillin-bindi...

journal_title：Current opinion in chemical biology

authors： Kluge AF,Petter RC

更新日期：2010-06-01 00:00:00

abstract：:Inhibition of the steroid 5alpha-reductases shows promise in the treatment of a number of androgen-dependent disorders, such as benign prostatic hyperplasia, male pattern baldness, and acne. The design of potent and isozyme-selective inhibitors has provided biologists and clinicians with important tools for elucidatin...

journal_title：Current opinion in chemical biology

authors： Harris GS,Kozarich JW

更新日期：1997-08-01 00:00:00

abstract：:Majority of eukaryotic proteins are glycosylated and their glycan moieties have numerous important structural, functional and regulatory roles. Because of structural complexity of glycans and technological limitations glycomics, and particularly glycoproteomics was not able to follow rapid progress in genomics and pro...

journal_title：Current opinion in chemical biology

authors： Zoldoš V,Horvat T,Lauc G

更新日期：2013-02-01 00:00:00

abstract：:New insights have been gained into the therapeutic relevance of the sphingosine 1-phosphate (S1P) pathway, on the basis of reverse pharmacological approaches to defining the mechanism of action of the immunosuppressive agent FTY720. Natural and synthetic sphingosine 1-phosphate receptor agonists can make picomolar int...

journal_title：Current opinion in chemical biology

authors： Rosen H,Liao J

更新日期：2003-08-01 00:00:00

abstract：:Fluorescence and reflectance spectroscopy provide the ability to assess tissue structure and metabolism in vivo in real time, providing improved diagnosis of pre-cancerous lesions. Reflectance spectroscopy can probe changes in epithelial nuclei that are important in pre-cancer detection, such as mean nuclear diameter,...

journal_title：Current opinion in chemical biology

authors： Sokolov K,Follen M,Richards-Kortum R

更新日期：2002-10-01 00:00:00

abstract：:Developing technologies such as unnatural amino acid mutagenesis, non-natural cofactor engineering, and computational design are generating proteins with novel functions; these proteins, however, often do not reach performance targets and would benefit from further optimization. Evolutionary methods can complement the...

journal_title：Current opinion in chemical biology

authors： Brustad EM,Arnold FH

更新日期：2011-04-01 00:00:00

abstract：:Modular peptide synthetases, which act as the protein templates for the synthesis of a large number of peptide antibiotics and siderophores, hold great potential for the development of novel compounds. Recently, significant progress has been made towards understanding their molecular architecture and substrate specifi...

journal_title：Current opinion in chemical biology

authors： Mootz HD,Marahiel MA

更新日期：1997-12-01 00:00:00

abstract：:The number of new chemical entities has remained rather constant (averaging 37 per year) in the past decade, despite the multiple-fold increase in the number of compounds that are being made and tested. Chemical space requires novel methods that can handle the increasing number of potentially accessible molecules. Nei...

journal_title：Current opinion in chemical biology

authors： Oprea TI

更新日期：2002-06-01 00:00:00

abstract：:Self-assembled monolayer (SAM) and fluorescent quantum dots (QDs) share common ground as emerging tools for nanoscale observation of biological interactions. SAMs provide excellent means of controlling the surface characteristics through individually tailored and engineered building blocks. SAMs on various surfaces ha...

journal_title：Current opinion in chemical biology

authors： Shin SK,Yoon HJ,Jung YJ,Park JW

更新日期：2006-10-01 00:00:00

abstract：:Initiating and terminating free-radical reactionis via hydrogen atom transfer (HAT) is an attractive means of avoiding substrate prefunctionalization. Small molecule catalysts and reagents, however, struggle to execute this fundamental step with useful levels of diastereoselectivity and enantioselectivity. In contrast...

journal_title：Current opinion in chemical biology

authors： Nakano Y,Biegasiewicz KF,Hyster TK

更新日期：2019-04-01 00:00:00

abstract：:DNA binding and transcriptional activation by basic region leucine zipper (bZIP) proteins can be enhanced by accessory factors. In the past year, three accessory factor-bZIP-DNA complexes have been investigated extensively. Experiments have probed the effect of accessory factors on bZIP-DNA specificity, the residues t...

journal_title：Current opinion in chemical biology

authors： Baranger AM

更新日期：1998-02-01 00:00:00

abstract：:The rapid growth of bio-sequence information has resulted in an increasing demand for reliable methods that group proteins. A few databases with curated alignments of protein families have demonstrated that expert-driven repositories can keep up with the data deluge in the genome era. These original resources implicit...

journal_title：Current opinion in chemical biology

authors： Liu J,Rost B

更新日期：2003-02-01 00:00:00

abstract：:Oligosaccharyl transferase is part of the macromolecular machinery that processes nascent proteins in the endoplasmic reticulum. The enzyme is highly conserved, catalyzes the initial step in the biosynthesis of N-linked glycoproteins and acts as a 'gatekeeper' for the secretory pathway. As more proteins associated wit...

journal_title：Current opinion in chemical biology

authors： Dempski RE Jr,Imperiali B

更新日期：2002-12-01 00:00:00

abstract：:Protein engineering over the past four years has made rhodopsin-based genetically encoded voltage indicators a leading candidate to achieve the task of reporting action potentials from a population of genetically targeted neurons in vivo. Rational design and large-scale screening efforts have steadily improved the dyn...

journal_title：Current opinion in chemical biology

authors： Gong Y

更新日期：2015-08-01 00:00:00

abstract：:Recent advances in membrane protein crystallography have greatly increased structural information of channels permeating metal ions. Structural bioinformatics techniques and molecular dynamics calculations are providing structural models of ion channels for which the three-dimensional structure is not known. Most of t...

journal_title：Current opinion in chemical biology

authors： Giorgetti A,Carloni P

更新日期：2003-02-01 00:00:00

abstract：:The ability of cobalamin to coordinate different upper axial ligands gives rise to a diversity of reactivity. Traditionally, adenosylcobalamin is associated with radical-based rearrangements, and methylcobalamin with methyl cation transfers. Recently, however, a new role for adenosylcobalamin has been discovered as a ...

journal_title：Current opinion in chemical biology

authors： Bridwell-Rabb J,Drennan CL

更新日期：2017-04-01 00:00:00

abstract：:Recent efforts in expanding the range of biofuel and biorenewable molecules using microbial production hosts have focused on the introduction of non-native pathways in model organisms and the bio-prospecting of non-model organisms with desirable features. Current challenges lie in the assembly and coordinated expressi...

journal_title：Current opinion in chemical biology

authors： Ng CY,Khodayari A,Chowdhury A,Maranas CD

更新日期：2015-10-01 00:00:00

abstract：:Technological advances in the automation of parallel synthesis are following the model set by the semiconductor industry: miniaturization, increasing speed, lower costs. Recent work includes preparation of high-density reaction blocks, development of ink-jet dispensing to polypropylene sheets and synthesis inside cust...

journal_title：Current opinion in chemical biology

authors： Cargill JF,Lebl M

更新日期：1997-06-01 00:00:00

abstract：:Genetically Encoded Voltage Indicators (GEVIs) are powerful tools used to investigate neural activity in the brain. The spatiotemporal resolution of GEVIs is on a subcellular and millisecond scale, and is superior to that of the functional magnetic resonance imaging (fMRI) and electroencephalogram (EEG). Further, whil...

journal_title：Current opinion in chemical biology

authors： Inagaki S,Nagai T

更新日期：2016-08-01 00:00:00