Interaction profiling methods to map protein and pathway targets of bioactive ligands.

abstract：:Chemical manipulation of natural, unengineered proteins is a daunting challenge which tests the limits of reaction design. By combining transition-metal or other catalysts with molecular recognition ideas, it is possible to achieve site-selective protein reactivity without the need for engineered recognition sequences...

journal_title：Current opinion in chemical biology

authors： Ball ZT

更新日期：2015-04-01 00:00:00

abstract：:KL4 (sinapultide) represents the first peptide-based replacement for surfactant protein B in pulmonary surfactant (PS) therapies approved for clinical use. Surfaxin, its formulation with PS lipids, shows the promise of synthetic PS for replacing animal-derived PS in the treatment of respiratory distress syndromes and ...

journal_title：Current opinion in chemical biology

authors： Braide-Moncoeur O,Tran NT,Long JR

更新日期：2016-06-01 00:00:00

abstract：:Thrombin is a multifunctional serine protease that plays a primary role in the pathogenic pathway of thrombosis as a consequence of its actions on the two principal components of blood clots, fibrin and activated platelets. Deficiencies in available therapies, heparin and coumadin, have recently led to an intense effo...

journal_title：Current opinion in chemical biology

authors： Ripka WC

更新日期：1997-08-01 00:00:00

abstract：:Recent advances in the development of the phosphorescent Ir(III) complexes have made it possible to implement the phosphorescence modality in bioimaging applications. A variety of phosphorescent Ir(III) complexes have been synthesized and assessed in the context of in vitro and in vivo imaging, especially in subcellul...

journal_title：Current opinion in chemical biology

authors： You Y

更新日期：2013-08-01 00:00:00

abstract：:Chemical imaging plays an increasingly important role in studying heterogeneous biological systems. It combines molecular spectroscopy with high-resolution spatial information to create quantitative images of molecular distributions. Here I summarize recent progress in technical developments and biological application...

journal_title：Current opinion in chemical biology

authors： Fu D

更新日期：2017-08-01 00:00:00

abstract：:Recent structural information suggests that the HC(X)5R active-site motif defines three distinct evolutionary families of phosphatases that employ a common catalytic mechanism. In two instances, regulation of phosphatase activity employs autoinhibitory mechanisms involving either intermolecular or intramolecular inter...

journal_title：Current opinion in chemical biology

authors： Denu JM,Dixon JE

更新日期：1998-10-01 00:00:00

abstract：:Protein synthesis is inherently a dynamic process, requiring both small-scale and large-scale movements of tRNA and mRNA. It has long been suspected that these movements might be coupled to conformational changes in the ribosome, and in its RNA moieties in particular. Recently, the nature of ribosome structural dynami...

journal_title：Current opinion in chemical biology

authors： Korostelev A,Ermolenko DN,Noller HF

更新日期：2008-12-01 00:00:00

abstract：:The number of new chemical entities has remained rather constant (averaging 37 per year) in the past decade, despite the multiple-fold increase in the number of compounds that are being made and tested. Chemical space requires novel methods that can handle the increasing number of potentially accessible molecules. Nei...

journal_title：Current opinion in chemical biology

authors： Oprea TI

更新日期：2002-06-01 00:00:00

abstract：:There is growing interest in the design of synthetic molecules that mimic the structures and functions of epitopes found on the surface of peptides and proteins. Epitope mimetics can provide valuable tools to probe complex biological processes, as well as interesting leads for drug and vaccine discovery. One applicati...

journal_title：Current opinion in chemical biology

authors： Robinson JA

更新日期：2011-06-01 00:00:00

abstract：:The field of computational protein design is reaching its adolescence. Protein design algorithms have been applied to design or engineer proteins that fold, fold faster, catalyze, catalyze faster, signal, and adopt preferred conformational states. Further developments of scoring functions, sampling strategies, and opt...

journal_title：Current opinion in chemical biology

authors： Kraemer-Pecore CM,Wollacott AM,Desjarlais JR

更新日期：2001-12-01 00:00:00

abstract：:Functional amino acids that play critical roles in catalysis and regulation are known to display elevated nucleophilicity and can be selectively targeted for covalent modification by reactive electrophiles. Chemical-proteomic platforms, such as activity-based protein profiling (ABPP), exploit this reactivity by utiliz...

journal_title：Current opinion in chemical biology

authors： Shannon DA,Weerapana E

更新日期：2015-02-01 00:00:00

abstract：:The biosynthetic pathways of plant natural products offer an abundance of knowledge to scientists in many fields. Synthetic chemists can be inspired by the synthetic strategies that nature uses to construct these compounds. Chemical and biological engineers are working to reprogram these biosynthetic pathways to more ...

journal_title：Current opinion in chemical biology

authors： Usera AR,O'Connor SE

更新日期：2009-10-01 00:00:00

abstract：:Different strategies for the creation of optical sensors with metal-dye complexes are discussed. The focus is on sensors, which are used in conjunction with pattern recognition protocols. It is shown that remarkably powerful sensors can be obtained by combining commercially available dyes with simple transition metal ...

journal_title：Current opinion in chemical biology

authors： Severin K

更新日期：2010-12-01 00:00:00

abstract：:The design of dimeric coiled-coils has ultimately led to novel applications, such as self-replicating peptide systems, whereas the structural features of the less common trimeric coiled-coil continue to be elucidated. Novel topologies have been discovered in designed proteins, as exemplified by the right-handed tetram...

journal_title：Current opinion in chemical biology

authors： Micklatcher C,Chmielewski J

更新日期：1999-12-01 00:00:00

abstract：:Systems modeling is emerging as a valuable tool in therapeutics. This is seen by the increasing use of clinically relevant computational models and a rise in systems biology companies working with the pharmaceutical industry. Systems models have helped understand the effects of pharmacological intervention at receptor...

journal_title：Current opinion in chemical biology

authors： Rajasethupathy P,Vayttaden SJ,Bhalla US

更新日期：2005-08-01 00:00:00

abstract：:Efficient library design is an ongoing challenge for investigators seeking novel ligands for proteins, whether for drug discovery or chemical biology. Strategies that add neglected chemistry or exclude unproductive compounds are two dominant recent themes, as is a growing awareness of molecular complexity and its impl...

journal_title：Current opinion in chemical biology

authors： Irwin JJ

更新日期：2006-08-01 00:00:00

abstract：:Monitoring the conformational dynamics of proteins is crucial for a better understanding of their biological functions. To observe the structural dynamics of proteins, it is often necessary to study each molecule individually. To this end, single-molecule techniques have been developed such as Förster resonance energy...

journal_title：Current opinion in chemical biology

authors： Frustaci S,Vollmer F

更新日期：2019-08-01 00:00:00

abstract：:The introduction of fluorine atoms into organic molecules has received considerable attention as these organofluorines have often found widespread applications in bioorganic chemistry, medicinal chemistry and biomaterial science. Despite innovation of synthetic C-F forming methodologies, selective fluorination is stil...

journal_title：Current opinion in chemical biology

authors： Wu L,Maglangit F,Deng H

更新日期：2020-04-01 00:00:00

abstract：:Lipopolysaccharide (LPS), a glycolipid found in the outer membrane of Gram-negative bacteria, is a potent elicitor of innate immune responses in mammals. A typical LPS molecule is composed of three different structural domains: a polysaccharide called the O-antigen, a core oligosaccharide, and Lipid A. Lipid A is the ...

journal_title：Current opinion in chemical biology

authors： Xiao X,Sankaranarayanan K,Khosla C

更新日期：2017-10-01 00:00:00

abstract：:Enzymes are nature's powerful catalytic proteins to perform reactions with often outstanding activity, selectivity and specificity. Moreover, the access to non-natural functions of biocatalysts can be facilitated by enzyme engineering. While rational approaches are often focused on an enzyme's active site, from random...

journal_title：Current opinion in chemical biology

authors： Kreß N,Halder JM,Rapp LR,Hauer B

更新日期：2018-12-01 00:00:00

abstract：:Synthetic biology offers a bottom-up engineering approach that intends to understand complex systems via design-build-test cycles. Embryonic development comprises complex processes that originate at the level of gene regulatory networks in a cell and emerge into collective cellular behaviors with multicellular forms a...

journal_title：Current opinion in chemical biology

authors： Ebrahimkhani MR,Ebisuya M

更新日期：2019-10-01 00:00:00

abstract：:Natural enzymes that catalyze Diels-Alder reactions have long been sought after, yet few enzymes have been experimentally confirmed to perform this reaction. In the past five years, several stand-alone enzymes that can catalyze the Diels-Alder reaction had been identified and characterized. Among which, the crystal st...

journal_title：Current opinion in chemical biology

authors： Hashimoto T,Kuzuyama T

更新日期：2016-12-01 00:00:00

abstract：:Nanoparticle-based therapeutics have received increasing attention, as these systems can alleviate many drawbacks of conventional therapy. Metal-organic frameworks (MOFs), a new class of hybrid materials composed of metal ions and organic bridging ligands, have emerged as a promising platform for drug delivery, owing ...

journal_title：Current opinion in chemical biology

authors： Huxford RC,Della Rocca J,Lin W

更新日期：2010-04-01 00:00:00

abstract：:Simple surfactants achieve remarkable long-range order in aqueous environments. This organizing potential is seen most dramatically in biological membranes where phospholipid assemblies both define cell boundaries and provide a ubiquitous structural scaffold for controlling cellular chemistry. Here we consider simple ...

journal_title：Current opinion in chemical biology

authors： Childers WS,Ni R,Mehta AK,Lynn DG

更新日期：2009-12-01 00:00:00

abstract：:Systematic studies of the organization of biochemical networks that make up the living cell can be defined by studying the organization and dynamics of protein interaction networks (PINs). Here, we describe recent conceptual and experimental advances that can achieve this aim and how chemical perturbations of interact...

journal_title：Current opinion in chemical biology

authors： Campbell-Valois FX,Michnick S

更新日期：2005-02-01 00:00:00

abstract：:Extracellular electron transfer via filamentous protein appendages called 'microbial nanowires' has long been studied in Geobacter and other bacteria because of their crucial role in globally-important environmental processes and their applications for bioenergy, biofuels, and bioelectronics. Thousands of papers thoug...

journal_title：Current opinion in chemical biology

authors： Yalcin SE,Malvankar NS

更新日期：2020-12-01 00:00:00

abstract：:Lysine acetylation has emerged as a key post-translational modification found at many sites throughout the cell. It plays an important role in epigenetic processes, and more generally in the regulation of protein stability and interactions. Acetyl groups are installed by lysine acetyltransferases and removed by lysine...

journal_title：Current opinion in chemical biology

authors： Schiedel M,Conway SJ

更新日期：2018-08-01 00:00:00

abstract：:The nature of the oxidized halogen intermediate in vanadium bromoperoxidase has recently been shown to depend on the nature of the organic substrate. For example, in the presence of indoles, vanadium bromoperoxidase does not release a freely diffusible oxidized halogen intermediate (such as HOBr+/-BR2+/-Br3-). Regiose...

journal_title：Current opinion in chemical biology

authors： Butler A

更新日期：1998-04-01 00:00:00

abstract：:The role of integrin and extracellular matrix proteins in various pathological processes (including angiogenesis, thrombosis, apoptosis and cell migration and proliferation), leading to both acute and chronic disease states (e.g. ocular diseases, metastasis, unstable angina, myocardial infarction, stroke, osteoporosis...

journal_title：Current opinion in chemical biology

authors： Mousa SA

更新日期：2002-08-01 00:00:00

abstract：:Quantitative microscopy is needed to understand reactions or phenomena carried out by biological molecules such as enzymes, receptors, and membrane-localized proteins. Counting the biomolecules of interest in single organelles or cellular compartments is critical in these approaches. In this brief perspective, we focu...

journal_title：Current opinion in chemical biology

authors： Jung SR,Fujimoto BS,Chiu DT

更新日期：2017-08-01 00:00:00