Protein tyrosine phosphatases: mechanisms of catalysis and regulation.

abstract：:RNA plays an essential cellular role in nearly every aspect of the transmission and expression of genetic information, including regulatory roles that have significance for cellular development. Access to RNA bearing synthetic modifications has allowed biological chemists to probe deep into the inner workings of cellu...

journal_title：Current opinion in chemical biology

authors： Das SR,Fong R,Piccirilli JA

更新日期：2005-12-01 00:00:00

abstract：:Over the past two decades, there has been intense interest in designing and implementing selective (bioorthogonal) reactions for biomolecule tracking. Here we review the most widely used bioorthogonal chemistries in live cells and animals, drawing particular attention to the unique functional groups underlying these t...

journal_title：Current opinion in chemical biology

authors： Shih HW,Kamber DN,Prescher JA

更新日期：2014-08-01 00:00:00

abstract：:Transition-metal catalysis has unlocked new paradigms for the late-stage modification and cyclization of peptides by harnessing the innate reactivity of proteinogenic amino acids. The field is rapidly evolving, with recent advances encompassing three fundamental areas-heteroatom couplings, decarboxylative cross-coupli...

journal_title：Current opinion in chemical biology

authors： Malins LR

更新日期：2018-10-01 00:00:00

abstract：:Simple surfactants achieve remarkable long-range order in aqueous environments. This organizing potential is seen most dramatically in biological membranes where phospholipid assemblies both define cell boundaries and provide a ubiquitous structural scaffold for controlling cellular chemistry. Here we consider simple ...

journal_title：Current opinion in chemical biology

authors： Childers WS,Ni R,Mehta AK,Lynn DG

更新日期：2009-12-01 00:00:00

abstract：:Sugar nucleotide-modifying enzymes of the short-chain dehydrogenase/reductase type use transient oxidation-reduction by a tightly bound nicotinamide cofactor as a common strategy of catalysis to promote a diverse set of reactions, including decarboxylation, single- or double-site epimerization, and dehydration. Althou...

journal_title：Current opinion in chemical biology

authors： Borg AJE,Beerens K,Pfeiffer M,Desmet T,Nidetzky B

更新日期：2020-11-06 00:00:00

abstract：:Synthetic biologists typically construct new pathways within existing cells. While useful, this approach in many ways ignores the undefined but necessary components of life. A growing number of laboratories have begun to try to remove some of the mysteries of cellular life by building life-like systems from non-living...

journal_title：Current opinion in chemical biology

authors： Forlin M,Lentini R,Mansy SS

更新日期：2012-12-01 00:00:00

abstract：:The past decade has seen rapid growth in the use of diverse compound libraries in classical phenotypic screens to identify modulators of a given process. The subsequent process of identifying the molecular targets of active hits, also called 'target deconvolution', is an essential step for understanding compound mecha...

journal_title：Current opinion in chemical biology

authors： Lee J,Bogyo M

更新日期：2013-02-01 00:00:00

abstract：:Inspired by advances in the ability to construct programmable circuits in living organisms, in vitro circuits are emerging as a viable platform for designing, understanding, and exploiting dynamic biochemical circuitry. In vitro systems allow researchers to directly access and manipulate biomolecular parts without the...

journal_title：Current opinion in chemical biology

authors： Hockenberry AJ,Jewett MC

更新日期：2012-08-01 00:00:00

abstract：:Nitrogenase catalyzes the important reactions of N2-reduction, CO-reduction and CO2-reduction at its active cofactor site. Designated the M-cluster, this complex metallocofactor is assembled through the generation of a characteristic 8Fe-core before the insertion of Mo and homocitrate that completes the stoichiometry ...

journal_title：Current opinion in chemical biology

authors： Hu Y,Ribbe MW

更新日期：2016-04-01 00:00:00

abstract：:Amide forming reactions are central to the field of peptide and protein synthesis and are considered to be poorly reversible reactions owing to the high stability of peptide bonds. One amide-forming reaction is native chemical ligation (NCL) which is driven by a sulfur to nitrogen acyl migration process from a transie...

journal_title：Current opinion in chemical biology

authors： Melnyk O,Agouridas V

更新日期：2014-10-01 00:00:00

abstract：:Molecular oxygen plays an indispensable role as a terminal electron acceptor in the electron transport chain in mitochondria. Acute or chronic oxygen deprivation (hypoxia) in organisms results in various diseases, and the elucidation of the pathogenic mechanism of hypoxia-related diseases and various cellular response...

journal_title：Current opinion in chemical biology

authors： Tobita S,Yoshihara T

更新日期：2016-08-01 00:00:00

abstract：:Four-electron reduction of dioxygen to water and splitting of water to dioxygen are extremely important processes in the context of attaining clean renewable energy sources. High-valent metal-oxo cores are proposed as reactive intermediates in these vital processes, although they have only been isolated in extremely r...

journal_title：Current opinion in chemical biology

authors： Ray K,Heims F,Schwalbe M,Nam W

更新日期：2015-04-01 00:00:00

abstract：:The site specific, co-translational introduction of unnatural amino acids into proteins produced in cells has been facilitated by the development of the pyrrolysyl-tRNA synthetase/tRNACUA pair. This pair can now be used to direct the site-specific incorporation of designer amino acids in E. coli, yeast, mammalian cell...

journal_title：Current opinion in chemical biology

authors： Elliott TS,Bianco A,Chin JW

更新日期：2014-08-01 00:00:00

abstract：:The first photocaged complexes appeared in the mid-1980s specifically to complement the ongoing work studying intracellular Ca(2+) signaling with fluorescent probes. Recently, the field of biological inorganic chemistry has focused increasingly on the homeostasis mechanisms and possible signaling functions of transiti...

journal_title：Current opinion in chemical biology

authors： Gwizdala C,Burdette SC

更新日期：2013-04-01 00:00:00

abstract：:In recent years, ogliopeptides have enjoyed ever increasing interest in two areas: first, approaches to biomimetic enzyme-like activity; and second, as metal-free catalysts for enantioselective transformations of synthetic interest. The discovery and optimization of peptides for these purposes has often used the metho...

journal_title：Current opinion in chemical biology

authors： Berkessel A

更新日期：2003-06-01 00:00:00

abstract：:Oxidative cysteine modifications have emerged as a central mechanism for dynamic post-translational regulation of all major protein classes and correlate with many disease states. Elucidating the precise roles of cysteine oxidation in physiology and pathology presents a major challenge. This article reviews the curren...

journal_title：Current opinion in chemical biology

authors： Leonard SE,Carroll KS

更新日期：2011-02-01 00:00:00

abstract：:Complex glycans participate in many essential life processes. Studies of glycan-mediated biological events have traditionally employed structurally defined fragments of the more elaborate natural molecules. However, it is now clear that this approach may sometimes be insufficient and this realization has prompted a de...

journal_title：Current opinion in chemical biology

authors： Lowary TL

更新日期：2013-12-01 00:00:00

abstract：:The notorious difficulty of elucidating structures of membrane transporters by crystallography has long prevented our understanding of active transport mechanism coupled with ion/proton transport. The determination of the first crystal structure of the drug/H+ antiporter AcrB was a breakthrough for structure-based und...

journal_title：Current opinion in chemical biology

authors： Tamura N,Konishi S,Yamaguchi A

更新日期：2003-10-01 00:00:00

abstract：:Developing technologies such as unnatural amino acid mutagenesis, non-natural cofactor engineering, and computational design are generating proteins with novel functions; these proteins, however, often do not reach performance targets and would benefit from further optimization. Evolutionary methods can complement the...

journal_title：Current opinion in chemical biology

authors： Brustad EM,Arnold FH

更新日期：2011-04-01 00:00:00

abstract：:Fluorescence and reflectance spectroscopy provide the ability to assess tissue structure and metabolism in vivo in real time, providing improved diagnosis of pre-cancerous lesions. Reflectance spectroscopy can probe changes in epithelial nuclei that are important in pre-cancer detection, such as mean nuclear diameter,...

journal_title：Current opinion in chemical biology

authors： Sokolov K,Follen M,Richards-Kortum R

更新日期：2002-10-01 00:00:00

abstract：:Cellular communication drives diverse aspects of organismal biology ranging from immune function to memory formation. The mechanisms by which cells transact information in vivo, though, are not completely understood. This is due, in part, to a lack of tools for observing collections of cells in their native habitats. ...

journal_title：Current opinion in chemical biology

authors： Porterfield WB,Prescher JA

更新日期：2015-02-01 00:00:00

abstract：:NMR, already some 50 years old, has long been an invaluable analytical method in industry for verification of chemical synthesis and compound characterisation. The range of molecular information accessible through NMR, however, offers a far larger horizon of applications. Of these, ligand screening by NMR has emerged ...

journal_title：Current opinion in chemical biology

authors： Diercks T,Coles M,Kessler H

更新日期：2001-06-01 00:00:00

abstract：:Fifty years after the publication of the DNA double helix model by Watson and Crick, new nucleic acid structures keep emerging at an ever-increasing rate. The past three years have brought a flurry of new oligonucleotide structures, including those of a Hoogsteen-paired DNA duplex, Holliday junctions, DNA-drug complex...

journal_title：Current opinion in chemical biology

authors： Egli M

更新日期：2004-12-01 00:00:00

abstract：:Chemical manipulation of natural, unengineered proteins is a daunting challenge which tests the limits of reaction design. By combining transition-metal or other catalysts with molecular recognition ideas, it is possible to achieve site-selective protein reactivity without the need for engineered recognition sequences...

journal_title：Current opinion in chemical biology

authors： Ball ZT

更新日期：2015-04-01 00:00:00

abstract：:Metallodrugs are extensively used to treat and diagnose distinct disease types. The unique physical-chemical properties of metal ions offer tantalizing opportunities to tailor effective scaffolds for selectively targeting specific biomolecules. Modern experimental techniques have collected a large body of structural d...

journal_title：Current opinion in chemical biology

authors： Janoš P,Spinello A,Magistrato A

更新日期：2020-08-08 00:00:00

abstract：:In the past five to ten years, it has become increasingly apparent that the function of Fe-S clusters is not limited to electron transfer, a function implicit in their discovery. We now know that the vulnerability of these structures to oxidative destruction is used by nature in sensing O2, iron, and possibly also nit...

journal_title：Current opinion in chemical biology

authors： Beinert H,Kiley PJ

更新日期：1999-04-01 00:00:00

abstract：:There is growing interest in the design of synthetic molecules that mimic the structures and functions of epitopes found on the surface of peptides and proteins. Epitope mimetics can provide valuable tools to probe complex biological processes, as well as interesting leads for drug and vaccine discovery. One applicati...

journal_title：Current opinion in chemical biology

authors： Robinson JA

更新日期：2011-06-01 00:00:00

abstract：:There are likely more than 500000 potential phosphorylation sites in a cellular proteome. This dynamic phosphorylation is under tight control of a variety of kinases and phosphatases. In recent years significant progress has been made in the large-scale analysis of these in vitro and in vivo protein phosphorylation ev...

journal_title：Current opinion in chemical biology

authors： Lemeer S,Heck AJ

更新日期：2009-10-01 00:00:00

abstract：:The field of computational protein design is reaching its adolescence. Protein design algorithms have been applied to design or engineer proteins that fold, fold faster, catalyze, catalyze faster, signal, and adopt preferred conformational states. Further developments of scoring functions, sampling strategies, and opt...

journal_title：Current opinion in chemical biology

authors： Kraemer-Pecore CM,Wollacott AM,Desjarlais JR

更新日期：2001-12-01 00:00:00

abstract：:This review explores the concept of using privileged scaffolds to identify biologically active compounds through building chemical libraries. We hope to accomplish three main objectives: to provide one of the most comprehensive listings of privileged scaffolds; to reveal through four selected examples the present stat...

journal_title：Current opinion in chemical biology

authors： Welsch ME,Snyder SA,Stockwell BR

更新日期：2010-06-01 00:00:00