Abstract:
:The site specific, co-translational introduction of unnatural amino acids into proteins produced in cells has been facilitated by the development of the pyrrolysyl-tRNA synthetase/tRNACUA pair. This pair can now be used to direct the site-specific incorporation of designer amino acids in E. coli, yeast, mammalian cells, and animals (the worm, C. elegans and the fly, D. melanogaster). Developments in encoding components of rapid bioorthogonal reactions are providing new opportunities for labelling and visualising proteins. A new method called stochastic orthogonal recoding of translation with chemoselective modification (SORT-M) leverages advances in genetic code expansion and bioorthogonal chemistry to label proteomes with diverse chemistry at diverse codons in E. coli, mammalian cells, and in spatially and temporally defined sets of cells in the fly. Proteomes in targeted sets of cells have been visualised by SORT-M and proteins in targeted cells have been identified by SORT-M.
journal_name
Curr Opin Chem Bioljournal_title
Current opinion in chemical biologyauthors
Elliott TS,Bianco A,Chin JWdoi
10.1016/j.cbpa.2014.07.001subject
Has Abstractpub_date
2014-08-01 00:00:00pages
154-60eissn
1367-5931issn
1879-0402pii
S1367-5931(14)00088-Xjournal_volume
21pub_type
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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