Advances in de novo strain design using integrated systems and synthetic biology tools.

abstract：:The catalytic pathways of soluble methane monooxygenase (sMMO) and cytochrome P450CAM, iron-containing enzymes, are described and compared. Recent extensive density functional ab initio electronic structure calculations have revealed many similarities in a number of the key catalytic steps, as well as some important d...

journal_title：Current opinion in chemical biology

authors： Guallar V,Gherman BF,Lippard SJ,Friesner RA

更新日期：2002-04-01 00:00:00

abstract：:Oligosaccharide recognition is a major means of bacterial-host cell attachment. Bacterial-host receptor binding can subvert host signaling pathways to cause pathology. In addition, pathogenic bacteria can utilize more than one recognition system to bind host cells. Recent studies of Helicobacter pylori illustrate both...

journal_title：Current opinion in chemical biology

authors： Lingwood CA

更新日期：1998-12-01 00:00:00

abstract：:Efficient library design is an ongoing challenge for investigators seeking novel ligands for proteins, whether for drug discovery or chemical biology. Strategies that add neglected chemistry or exclude unproductive compounds are two dominant recent themes, as is a growing awareness of molecular complexity and its impl...

journal_title：Current opinion in chemical biology

authors： Irwin JJ

更新日期：2006-08-01 00:00:00

abstract：:The ability of cobalamin to coordinate different upper axial ligands gives rise to a diversity of reactivity. Traditionally, adenosylcobalamin is associated with radical-based rearrangements, and methylcobalamin with methyl cation transfers. Recently, however, a new role for adenosylcobalamin has been discovered as a ...

journal_title：Current opinion in chemical biology

authors： Bridwell-Rabb J,Drennan CL

更新日期：2017-04-01 00:00:00

abstract：:Signal amplification in conventional enzyme-based biosensors is not high enough to achieve the ultrasensitive detection of biomolecules. In recent years, signal amplification has been improved by combining enzymatic reactions with redox cycling or employing multienzyme labels per detection probe. Electrochemical-chemi...

journal_title：Current opinion in chemical biology

authors： Yang H

更新日期：2012-08-01 00:00:00

abstract：:Altered metabolite levels can drive epigenetic changes critical to development and disease. However, in many cases the specific protein-metabolite interactions that underlie this process remain enigmatic. In this review, we make the case that this fundamental missing information may be discovered by applying the tools...

journal_title：Current opinion in chemical biology

authors： Kulkarni RA,Montgomery DC,Meier JL

更新日期：2019-08-01 00:00:00

abstract：:Phosphorus cycling in the biosphere has traditionally been thought to involve almost exclusively transformations of the element in its pentavalent oxidation state. Recent evidence, however, suggests that a significant fraction of environmental phosphorus may exist in a more reduced form. Most abundant of these reduced...

journal_title：Current opinion in chemical biology

authors： Chin JP,McGrath JW,Quinn JP

更新日期：2016-04-01 00:00:00

abstract：:Natural evolution has produced a great diversity of proteins that can be harnessed for numerous applications in biotechnology and pharmaceutical science. Commonly, specific applications require proteins to be tailored by protein engineering. Directed evolution is a type of protein engineering that yields proteins with...

journal_title：Current opinion in chemical biology

authors： Lane MD,Seelig B

更新日期：2014-10-01 00:00:00

abstract：:Compartmentalization is one of the defining features of life. Cells use protein compartments to exert spatial control over their metabolism, store nutrients and create unique microenvironments needed for essential physiological processes. Encapsulins are a recently discovered class of protein nanocompartments found in...

journal_title：Current opinion in chemical biology

authors： Giessen TW

更新日期：2016-10-01 00:00:00

abstract：:In recent years pharmaceutical companies have utilized structure-based drug design and combinatorial library design techniques to speed up their drug discovery efforts. Both approaches are routinely used in the lead discovery and lead optimization stages of the drug discovery process. Fragment-based drug design, a new...

journal_title：Current opinion in chemical biology

authors： Zhou JZ

更新日期：2008-06-01 00:00:00

abstract：:Systematic studies of the organization of biochemical networks that make up the living cell can be defined by studying the organization and dynamics of protein interaction networks (PINs). Here, we describe recent conceptual and experimental advances that can achieve this aim and how chemical perturbations of interact...

journal_title：Current opinion in chemical biology

authors： Campbell-Valois FX,Michnick S

更新日期：2005-02-01 00:00:00

abstract：:Sugar nucleotide-modifying enzymes of the short-chain dehydrogenase/reductase type use transient oxidation-reduction by a tightly bound nicotinamide cofactor as a common strategy of catalysis to promote a diverse set of reactions, including decarboxylation, single- or double-site epimerization, and dehydration. Althou...

journal_title：Current opinion in chemical biology

authors： Borg AJE,Beerens K,Pfeiffer M,Desmet T,Nidetzky B

更新日期：2020-11-06 00:00:00

abstract：:Quantitative microscopy is needed to understand reactions or phenomena carried out by biological molecules such as enzymes, receptors, and membrane-localized proteins. Counting the biomolecules of interest in single organelles or cellular compartments is critical in these approaches. In this brief perspective, we focu...

journal_title：Current opinion in chemical biology

authors： Jung SR,Fujimoto BS,Chiu DT

更新日期：2017-08-01 00:00:00

abstract：:In recent years, ogliopeptides have enjoyed ever increasing interest in two areas: first, approaches to biomimetic enzyme-like activity; and second, as metal-free catalysts for enantioselective transformations of synthetic interest. The discovery and optimization of peptides for these purposes has often used the metho...

journal_title：Current opinion in chemical biology

authors： Berkessel A

更新日期：2003-06-01 00:00:00

abstract：:Genetically Encoded Voltage Indicators (GEVIs) are powerful tools used to investigate neural activity in the brain. The spatiotemporal resolution of GEVIs is on a subcellular and millisecond scale, and is superior to that of the functional magnetic resonance imaging (fMRI) and electroencephalogram (EEG). Further, whil...

journal_title：Current opinion in chemical biology

authors： Inagaki S,Nagai T

更新日期：2016-08-01 00:00:00

abstract：:Nanoparticle-based therapeutics have received increasing attention, as these systems can alleviate many drawbacks of conventional therapy. Metal-organic frameworks (MOFs), a new class of hybrid materials composed of metal ions and organic bridging ligands, have emerged as a promising platform for drug delivery, owing ...

journal_title：Current opinion in chemical biology

authors： Huxford RC,Della Rocca J,Lin W

更新日期：2010-04-01 00:00:00

abstract：:During the past year, remarkable progress has been made in understanding how periplasmic chaperones fold and protect protein modules that are destined for assembly into adhesive pili in Gram-negative bacteria. The first two three-dimensional structures of complexes of periplasmic chaperones with substrate pilus subuni...

journal_title：Current opinion in chemical biology

authors： Knight SD,Berglund J,Choudhury D

更新日期：2000-12-01 00:00:00

abstract：:The number of new chemical entities has remained rather constant (averaging 37 per year) in the past decade, despite the multiple-fold increase in the number of compounds that are being made and tested. Chemical space requires novel methods that can handle the increasing number of potentially accessible molecules. Nei...

journal_title：Current opinion in chemical biology

authors： Oprea TI

更新日期：2002-06-01 00:00:00

abstract：:Nitrogenase catalyzes the important reactions of N2-reduction, CO-reduction and CO2-reduction at its active cofactor site. Designated the M-cluster, this complex metallocofactor is assembled through the generation of a characteristic 8Fe-core before the insertion of Mo and homocitrate that completes the stoichiometry ...

journal_title：Current opinion in chemical biology

authors： Hu Y,Ribbe MW

更新日期：2016-04-01 00:00:00

abstract：:A variety of protein sequence databases exist, ranging from simple sequence repositories, which store data with little or no manual intervention in the creation of the records, to expertly curated universal databases that cover all species and in which the original sequence data are enhanced by the manual addition of ...

journal_title：Current opinion in chemical biology

authors： Apweiler R,Bairoch A,Wu CH

更新日期：2004-02-01 00:00:00

abstract：:Lysine acetylation has emerged as a key post-translational modification found at many sites throughout the cell. It plays an important role in epigenetic processes, and more generally in the regulation of protein stability and interactions. Acetyl groups are installed by lysine acetyltransferases and removed by lysine...

journal_title：Current opinion in chemical biology

authors： Schiedel M,Conway SJ

更新日期：2018-08-01 00:00:00

abstract：:The introduction of fluorine atoms into organic molecules has received considerable attention as these organofluorines have often found widespread applications in bioorganic chemistry, medicinal chemistry and biomaterial science. Despite innovation of synthetic C-F forming methodologies, selective fluorination is stil...

journal_title：Current opinion in chemical biology

authors： Wu L,Maglangit F,Deng H

更新日期：2020-04-01 00:00:00

abstract：:Bacterial-derived antimicrobial polypeptides enjoy a large degree of structural and chemical diversity. Two well-studied examples of such polypeptides are the lanthionine-containing lantibiotics produced by a variety of Gram-positive bacteria, and their Gram-negative counterparts, the microcins. Both groups are produc...

journal_title：Current opinion in chemical biology

authors： Jack RW,Jung G

更新日期：2000-06-01 00:00:00

abstract：:The rapid growth of bio-sequence information has resulted in an increasing demand for reliable methods that group proteins. A few databases with curated alignments of protein families have demonstrated that expert-driven repositories can keep up with the data deluge in the genome era. These original resources implicit...

journal_title：Current opinion in chemical biology

authors： Liu J,Rost B

更新日期：2003-02-01 00:00:00

abstract：:The molecular mechanisms by which peptide antibiotics disrupt bacterial DNA synthesis, protein biosynthesis, cell wall biosynthesis, and membrane integrity are diverse, yet historically have been understood to follow a theme of one antibiotic, one inhibitory mechanism. In the past year, mechanistic and structural stud...

journal_title：Current opinion in chemical biology

authors： McCafferty DG,Cudic P,Yu MK,Behenna DC,Kruger R

更新日期：1999-12-01 00:00:00

abstract：:Four-electron reduction of dioxygen to water and splitting of water to dioxygen are extremely important processes in the context of attaining clean renewable energy sources. High-valent metal-oxo cores are proposed as reactive intermediates in these vital processes, although they have only been isolated in extremely r...

journal_title：Current opinion in chemical biology

authors： Ray K,Heims F,Schwalbe M,Nam W

更新日期：2015-04-01 00:00:00

abstract：:The principles of enzyme chemistry, mechanism of action and inhibitor design are being applied to proteomics by the development of activity-based probes. This approach suggests a potentially broad method for interrogating enzyme family members, both known and unknown, in cells and proteomic fractions without the need ...

journal_title：Current opinion in chemical biology

authors： Kozarich JW

更新日期：2003-02-01 00:00:00

abstract：:In recent years, an increasing number of proteins have been discovered which utilize heme cofactors to sense oxygen, carbon monoxide and nitric oxide. The identification and characterization of these proteins are revising our understanding of heme-mediated allostery first established in the early 1960s. Biochemical an...

journal_title：Current opinion in chemical biology

authors： Chan MK

更新日期：2001-04-01 00:00:00

abstract：:Small molecule production in microbial hosts is limited by the accumulation of the product inside the cell. Efflux transporters show promise as a solution to removal of the often-toxic products. Recent advances in transporter identification through expression profiling, heterologous expression, and knockout studies ha...

journal_title：Current opinion in chemical biology

authors： Boyarskiy S,Tullman-Ercek D

更新日期：2015-10-01 00:00:00

abstract：:New insights have been gained into the formation of the di-iron tyrosyl radical cofactor, which is essential for nucleotide reduction in the class I ribonucleotide reductases. Recent advances include Insight into the function of the tyrosyl radical in initiation of nucleotide reduction. ...

journal_title：Current opinion in chemical biology

authors： Stubbe J

更新日期：2003-04-01 00:00:00