Getting pumped: membrane efflux transporters for enhanced biomolecule production.

abstract：:Fragment-based screening has now become an established method for the generation of lead molecules against therapeutic targets. Fragment molecules are simple, low molecular-weight compounds with few chemical functionalities. These characteristics lead to high hit rates for fragment screening as compared to the more cl...

journal_title：Current opinion in chemical biology

authors： Barelier S,Krimm I

更新日期：2011-08-01 00:00:00

abstract：:The past decade has seen rapid growth in the use of diverse compound libraries in classical phenotypic screens to identify modulators of a given process. The subsequent process of identifying the molecular targets of active hits, also called 'target deconvolution', is an essential step for understanding compound mecha...

journal_title：Current opinion in chemical biology

authors： Lee J,Bogyo M

更新日期：2013-02-01 00:00:00

abstract：:The principles of enzyme chemistry, mechanism of action and inhibitor design are being applied to proteomics by the development of activity-based probes. This approach suggests a potentially broad method for interrogating enzyme family members, both known and unknown, in cells and proteomic fractions without the need ...

journal_title：Current opinion in chemical biology

authors： Kozarich JW

更新日期：2003-02-01 00:00:00

abstract：:Inspired by advances in the ability to construct programmable circuits in living organisms, in vitro circuits are emerging as a viable platform for designing, understanding, and exploiting dynamic biochemical circuitry. In vitro systems allow researchers to directly access and manipulate biomolecular parts without the...

journal_title：Current opinion in chemical biology

authors： Hockenberry AJ,Jewett MC

更新日期：2012-08-01 00:00:00

abstract：:The range and diversity of solid-phase synthetic methodology continues to develop very rapidly, allowing the generation of previously inaccessible compound libraries. The design of such libraries requires consideration of linkage strategies, newly developed solid-phase reactions and emerging solution-phase processes. ...

journal_title：Current opinion in chemical biology

authors： Oliver SF,Abell C

更新日期：1999-06-01 00:00:00

abstract：:Initiating and terminating free-radical reactionis via hydrogen atom transfer (HAT) is an attractive means of avoiding substrate prefunctionalization. Small molecule catalysts and reagents, however, struggle to execute this fundamental step with useful levels of diastereoselectivity and enantioselectivity. In contrast...

journal_title：Current opinion in chemical biology

authors： Nakano Y,Biegasiewicz KF,Hyster TK

更新日期：2019-04-01 00:00:00

abstract：:Recent studies of moonlighting functions and catalytic promiscuity provide insights into the structural and mechanistic bases of these phenomena. Moonlighting proteins that are highlighted include gephyrin, the Neurospora crassa tyrosyl tRNA synthetase, phosphoglucose isomerase, and cytochrome c. New insights into cat...

journal_title：Current opinion in chemical biology

authors： Copley SD

更新日期：2003-04-01 00:00:00

abstract：:Quantitative microscopy is needed to understand reactions or phenomena carried out by biological molecules such as enzymes, receptors, and membrane-localized proteins. Counting the biomolecules of interest in single organelles or cellular compartments is critical in these approaches. In this brief perspective, we focu...

journal_title：Current opinion in chemical biology

authors： Jung SR,Fujimoto BS,Chiu DT

更新日期：2017-08-01 00:00:00

abstract：:Majority of eukaryotic proteins are glycosylated and their glycan moieties have numerous important structural, functional and regulatory roles. Because of structural complexity of glycans and technological limitations glycomics, and particularly glycoproteomics was not able to follow rapid progress in genomics and pro...

journal_title：Current opinion in chemical biology

authors： Zoldoš V,Horvat T,Lauc G

更新日期：2013-02-01 00:00:00

abstract：:Recent advances in the development of the phosphorescent Ir(III) complexes have made it possible to implement the phosphorescence modality in bioimaging applications. A variety of phosphorescent Ir(III) complexes have been synthesized and assessed in the context of in vitro and in vivo imaging, especially in subcellul...

journal_title：Current opinion in chemical biology

authors： You Y

更新日期：2013-08-01 00:00:00

abstract：:The industrial application of enzymes that can withstand harsh conditions has greatly increased over the past decade. This is mainly a result of the discovery of novel enzymes from extremophilic microorganisms. Recent advances in the study of extremozymes point to the acceleration of this trend. In particular, enzymes...

journal_title：Current opinion in chemical biology

authors： Demirjian DC,Morís-Varas F,Cassidy CS

更新日期：2001-04-01 00:00:00

abstract：:Given the correlation between many human diseases and mis-regulated transcription, there is a growing need for molecules that can inhibit or mimic key interactions between transcriptional activators and their binding partners. Because transcriptional activators typically participate in many different protein-protein b...

journal_title：Current opinion in chemical biology

authors： Majmudar CY,Mapp AK

更新日期：2005-10-01 00:00:00

abstract：:Although biotin and lipoic acid are two universally conserved cofactors essential for intermediary metabolism, their synthetic pathways have become known only in recent years. Both pathways have unusual features. Biotin synthesis in Escherichia coli requires a methylation that is later removed whereas lipoic acid is a...

journal_title：Current opinion in chemical biology

authors： Cronan JE

更新日期：2018-12-01 00:00:00

abstract：:A variety of protein sequence databases exist, ranging from simple sequence repositories, which store data with little or no manual intervention in the creation of the records, to expertly curated universal databases that cover all species and in which the original sequence data are enhanced by the manual addition of ...

journal_title：Current opinion in chemical biology

authors： Apweiler R,Bairoch A,Wu CH

更新日期：2004-02-01 00:00:00

abstract：:Elastin-like polypeptides (ELPs) are artificial polypeptides, derived from Val-Pro-Gly-Xaa-Gly (VPGXG) pentapeptide repeats found in human tropoelastin, that reversibly coacervate above a critical temperature. Genetically encodable ELPs are monodisperse, stimuli responsive, and biocompatible, properties that make them...

journal_title：Current opinion in chemical biology

authors： Chilkoti A,Christensen T,MacKay JA

更新日期：2006-12-01 00:00:00

abstract：:A broad range of biochemicals, from proteins to nucleic acids, function properly only when associated with a metal, usually a divalent cation. Not any divalent metal will do: these metals differ in their ionic radius, dissociation in water, ionization potential, and number of unpaired electrons in their outer shells, ...

journal_title：Current opinion in chemical biology

authors： Bloom AJ

更新日期：2019-04-01 00:00:00

abstract：:During the past year, remarkable progress has been made in understanding how periplasmic chaperones fold and protect protein modules that are destined for assembly into adhesive pili in Gram-negative bacteria. The first two three-dimensional structures of complexes of periplasmic chaperones with substrate pilus subuni...

journal_title：Current opinion in chemical biology

authors： Knight SD,Berglund J,Choudhury D

更新日期：2000-12-01 00:00:00

abstract：:Immunoassays are indispensable for research and clinical analysis, and following the emergence of the omics paradigm, multiplexing of immunoassays is more needed than ever. Cross-reactivity (CR) in multiplexed immunoassays has been unexpectedly difficult to mitigate, preventing scaling up of multiplexing, limiting ass...

journal_title：Current opinion in chemical biology

authors： Juncker D,Bergeron S,Laforte V,Li H

更新日期：2014-02-01 00:00:00

abstract：:Thrombin is a multifunctional serine protease that plays a primary role in the pathogenic pathway of thrombosis as a consequence of its actions on the two principal components of blood clots, fibrin and activated platelets. Deficiencies in available therapies, heparin and coumadin, have recently led to an intense effo...

journal_title：Current opinion in chemical biology

authors： Ripka WC

更新日期：1997-08-01 00:00:00

abstract：:Herein we review contemporary synthetic and protein design strategies to stabilize the alpha-helical motif in short peptides and miniature proteins. Advances in organometallic catalyst design, specifically for the olefin metathesis reaction, enable the use of hydrocarbon bridges to either crosslink side chains of spec...

journal_title：Current opinion in chemical biology

authors： Henchey LK,Jochim AL,Arora PS

更新日期：2008-12-01 00:00:00

abstract：:The nature of the oxidized halogen intermediate in vanadium bromoperoxidase has recently been shown to depend on the nature of the organic substrate. For example, in the presence of indoles, vanadium bromoperoxidase does not release a freely diffusible oxidized halogen intermediate (such as HOBr+/-BR2+/-Br3-). Regiose...

journal_title：Current opinion in chemical biology

authors： Butler A

更新日期：1998-04-01 00:00:00

abstract：:Iron-binding transcription factors are widespread throughout the bacterial world and to date are known to bind several types of cofactors, such as Fe2+, heme, or iron-sulfur clusters. The known chemistry of these cofactors is exploited by transcription factors, including Fur, FNR, and NsrR, to sense molecules such as ...

journal_title：Current opinion in chemical biology

authors： Fleischhacker AS,Kiley PJ

更新日期：2011-04-01 00:00:00

abstract：:The appearance of the highly pathogenic avian influenza virus H5N1 highlighted the potential impact of influenza virus on humanity. The emergence of this high profile virus stimulated much research towards a better understanding of the key determinants for successful human-to-human transmission and as such has provide...

journal_title：Current opinion in chemical biology

authors： von Itzstein M

更新日期：2008-02-01 00:00:00

abstract：:The active site of cross-linked enzyme crystals can be further modified by chemical means, yielding a new type of chemically engineered enzyme. In the past year, the characteristics of cross-linked protein crystals as microporous materials have been studied and used as stationary phases in chromatographic separations....

journal_title：Current opinion in chemical biology

authors： Häring D,Schreier P

更新日期：1999-02-01 00:00:00

abstract：:Conceptual and computational advances triggered an explosion of designed protein structures in the recent years. Various protein fold geometries have been robustly designed with atomic accuracy, including protein folds unseen in nature. The same principles and tools have been extended to design multi-chain assemblies....

journal_title：Current opinion in chemical biology

authors： Ljubetič A,Gradišar H,Jerala R

更新日期：2017-10-01 00:00:00

abstract：:Major advances have been made in our understanding of cytochrome c oxidase owing to continued crystallographic work on important intermediates. This, together with a wealth of data derived from selective mutations and sophisticated spectroscopic probes, has provided significant new insights into oxidase dioxygen chemi...

journal_title：Current opinion in chemical biology

authors： Poulos TL,Li H,Raman CS

更新日期：1999-04-01 00:00:00

abstract：:Because biomarkers are typically low in abundance, the crucial step of biomarker discovery is to efficiently separate clinically relevant sets of proteins that might define disease stages and/or predict disease development. It is anticipated that a multi-dimensional fractionation system (MDFS) will provide an efficien...

journal_title：Current opinion in chemical biology

authors： Lee HJ,Lee EY,Kwon MS,Paik YK

更新日期：2006-02-01 00:00:00

abstract：:Natural products are evolutionarily designed and chemically distinct from most synthetic library molecules. In addition to their role as drugs, they are successfully used as molecular probes to identify disease relevant targets. Novel natural products are still routinely discovered from traditional sources through cul...

journal_title：Current opinion in chemical biology

authors： Schmitt EK,Moore CM,Krastel P,Petersen F

更新日期：2011-08-01 00:00:00

abstract：:Altered metabolite levels can drive epigenetic changes critical to development and disease. However, in many cases the specific protein-metabolite interactions that underlie this process remain enigmatic. In this review, we make the case that this fundamental missing information may be discovered by applying the tools...

journal_title：Current opinion in chemical biology

authors： Kulkarni RA,Montgomery DC,Meier JL

更新日期：2019-08-01 00:00:00

abstract：:Structural genomics (SG) has significantly increased the number of novel protein structures of targets with medical relevance. In the protein kinase area, SG has contributed >50% of all novel kinases structures during the past three years and determined more than 30 novel catalytic domain structures. Many of the relea...

journal_title：Current opinion in chemical biology

authors： Marsden BD,Knapp S

更新日期：2008-02-01 00:00:00