Abstract:
:Because biomarkers are typically low in abundance, the crucial step of biomarker discovery is to efficiently separate clinically relevant sets of proteins that might define disease stages and/or predict disease development. It is anticipated that a multi-dimensional fractionation system (MDFS) will provide an efficient means of separating low abundance proteins from plasma proteins, resulting in the extension of the detection limit. However, when using an MDFS to analyze the plasma proteome it is important to consider how sample processing, yield, resolution and throughput potential may influence the detection limit. This review evaluates the recent advances in MDFS research with respect to '4RS criterion' (4R: resolution, reproducibility, recovery, and robustness; 4S: simplicity, speed, selectivity and sensitivity) and discusses perspectives for future plasma-derived biomarker discovery.
journal_name
Curr Opin Chem Bioljournal_title
Current opinion in chemical biologyauthors
Lee HJ,Lee EY,Kwon MS,Paik YKdoi
10.1016/j.cbpa.2006.01.007subject
Has Abstractpub_date
2006-02-01 00:00:00pages
42-9issue
1eissn
1367-5931issn
1879-0402pii
S1367-5931(06)00006-8journal_volume
10pub_type
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journal_title:Current opinion in chemical biology
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