Vanadium haloperoxidases.

abstract：:Nucleic acids such as DNA are increasingly being applied in nanotechnology, as a result of their capability to self-assemble reversibly. The formal replacement of canonical base pairs by metal-mediated ones enables a site-specific introduction of metal-based functionality into these biomolecules, leading to the format...

journal_title：Current opinion in chemical biology

authors： Mandal S,Müller J

更新日期：2017-04-01 00:00:00

abstract：:Efficient library design is an ongoing challenge for investigators seeking novel ligands for proteins, whether for drug discovery or chemical biology. Strategies that add neglected chemistry or exclude unproductive compounds are two dominant recent themes, as is a growing awareness of molecular complexity and its impl...

journal_title：Current opinion in chemical biology

authors： Irwin JJ

更新日期：2006-08-01 00:00:00

abstract：:To date, the vast majority of the proteomic data sets collected by mass spectrometry (MS) have been generated by nondirected methods, whereby the identified precursor ions are stochastically selected for sequencing from complex sample mixtures. Recently, new MS approaches have been developed in which the mass spectrom...

journal_title：Current opinion in chemical biology

authors： Schmidt A,Claassen M,Aebersold R

更新日期：2009-12-01 00:00:00

abstract：:Recently, the hypothesis that protein motions are involved in enzymatic turnover has gained significant attention. We review cases where there is evidence that protein motions are rate-limiting in the overall catalytic cycle and examine experimental and theoretical evidence for how such motions enhance the probability...

journal_title：Current opinion in chemical biology

authors： Nashine VC,Hammes-Schiffer S,Benkovic SJ

更新日期：2010-10-01 00:00:00

abstract：:Sugar nucleotide-modifying enzymes of the short-chain dehydrogenase/reductase type use transient oxidation-reduction by a tightly bound nicotinamide cofactor as a common strategy of catalysis to promote a diverse set of reactions, including decarboxylation, single- or double-site epimerization, and dehydration. Althou...

journal_title：Current opinion in chemical biology

authors： Borg AJE,Beerens K,Pfeiffer M,Desmet T,Nidetzky B

更新日期：2020-11-06 00:00:00

abstract：:Recent efforts in expanding the range of biofuel and biorenewable molecules using microbial production hosts have focused on the introduction of non-native pathways in model organisms and the bio-prospecting of non-model organisms with desirable features. Current challenges lie in the assembly and coordinated expressi...

journal_title：Current opinion in chemical biology

authors： Ng CY,Khodayari A,Chowdhury A,Maranas CD

更新日期：2015-10-01 00:00:00

abstract：:Lipids are a vital class of molecules that play important and varied roles in biological processes, however, fully understanding these roles is extremely difficult due to the immense number and diversity of possible lipid species. While recent advances in chromatography and high resolution mass spectrometry have great...

journal_title：Current opinion in chemical biology

authors： Zheng X,Smith RD,Baker ES

更新日期：2018-02-01 00:00:00

abstract：:During the past year, remarkable progress has been made in understanding how periplasmic chaperones fold and protect protein modules that are destined for assembly into adhesive pili in Gram-negative bacteria. The first two three-dimensional structures of complexes of periplasmic chaperones with substrate pilus subuni...

journal_title：Current opinion in chemical biology

authors： Knight SD,Berglund J,Choudhury D

更新日期：2000-12-01 00:00:00

abstract：:Peptides that recognize specific cell types promise to be valuable tools both in research and clinical applications. Cell-specific peptides can be useful as drug delivery vehicles, diagnostic agents, affinity reagents for cell purification, gene therapy delivery agents, and research tools to probe the nature of a cell...

journal_title：Current opinion in chemical biology

authors： Brown KC

更新日期：2000-02-01 00:00:00

abstract：:Bacterial-derived antimicrobial polypeptides enjoy a large degree of structural and chemical diversity. Two well-studied examples of such polypeptides are the lanthionine-containing lantibiotics produced by a variety of Gram-positive bacteria, and their Gram-negative counterparts, the microcins. Both groups are produc...

journal_title：Current opinion in chemical biology

authors： Jack RW,Jung G

更新日期：2000-06-01 00:00:00

abstract：:Oligonucleotide in vitro selection provides surprisingly specific aptamers to protein targets. Synthetic chemistries for modifying nucleotides have been developed to enhance aptamer binding affinity. The diversity of nucleosides amenable to either triphosphate synthesis or phosphoramidite activation is now sufficientl...

journal_title：Current opinion in chemical biology

authors： Eaton BE

更新日期：1997-06-01 00:00:00

abstract：:Synthetic biologists typically construct new pathways within existing cells. While useful, this approach in many ways ignores the undefined but necessary components of life. A growing number of laboratories have begun to try to remove some of the mysteries of cellular life by building life-like systems from non-living...

journal_title：Current opinion in chemical biology

authors： Forlin M,Lentini R,Mansy SS

更新日期：2012-12-01 00:00:00

abstract：:The translation of nucleic acids into synthetic structures with expanded functional potential has been the subject of considerable research, with applications including small-molecule and polymer evolution, reaction discovery and sensing. Here, we review properties of nucleic-acid-templated synthesis in the context of...

journal_title：Current opinion in chemical biology

authors： Calderone CT,Liu DR

更新日期：2004-12-01 00:00:00

abstract：:Combinatorial chemistry methods and high-throughput screening for leads in industrial drug discovery have generated a potential bottleneck in the optimisation processes that seek to align potency with good pharmacokinetics in order to produce good medicines. This has resulted in the need for higher throughput methods ...

journal_title：Current opinion in chemical biology

authors： Tarbit MH,Berman J

更新日期：1998-06-01 00:00:00

abstract：:Biosensors use biological elements to detect or quantify an analyte of interest. In bioprocessing, biosensors are employed to monitor key metabolites. There are two main types: fully biological systems or biological recognition coupled with physical/chemical detection. New developments in chemical biosensors include m...

journal_title：Current opinion in chemical biology

authors： Dekker L,Polizzi KM

更新日期：2017-10-01 00:00:00

abstract：:Small molecule production in microbial hosts is limited by the accumulation of the product inside the cell. Efflux transporters show promise as a solution to removal of the often-toxic products. Recent advances in transporter identification through expression profiling, heterologous expression, and knockout studies ha...

journal_title：Current opinion in chemical biology

authors： Boyarskiy S,Tullman-Ercek D

更新日期：2015-10-01 00:00:00

abstract：:Developing technologies such as unnatural amino acid mutagenesis, non-natural cofactor engineering, and computational design are generating proteins with novel functions; these proteins, however, often do not reach performance targets and would benefit from further optimization. Evolutionary methods can complement the...

journal_title：Current opinion in chemical biology

authors： Brustad EM,Arnold FH

更新日期：2011-04-01 00:00:00

abstract：:The reactive oxygen and nitrogen species superoxide ion, nitric oxide, nitrogen dioxide and peroxynitrite ion all react with biological target molecules. Some of these interactions are carefully orchestrated segments of signal transduction cascades or part of the armamentarium of the immune system, others are patholog...

journal_title：Current opinion in chemical biology

authors： Groves JT

更新日期：1999-04-01 00:00:00

abstract：:Compartmentalization is one of the defining features of life. Cells use protein compartments to exert spatial control over their metabolism, store nutrients and create unique microenvironments needed for essential physiological processes. Encapsulins are a recently discovered class of protein nanocompartments found in...

journal_title：Current opinion in chemical biology

authors： Giessen TW

更新日期：2016-10-01 00:00:00

abstract：:Methanogenesis from trimethylamine, dimethylamine or monomethylamine is initiated by a series of corrinoid-dependent methyltransferases. The non-homologous genes encoding the full-length methyltransferases each possess an in-frame UAG (amber) codon that does not terminate translation. The amber codon is decoded by a d...

journal_title：Current opinion in chemical biology

authors： Krzycki JA

更新日期：2004-10-01 00:00:00

abstract：:Peptide nucleic acid (PNA)-encoded chemical libraries along with DNA-encoded libraries have provided a powerful new paradigm for library synthesis and ligand discovery. PNA-encoding stands out for its compatibility with standard solid phase synthesis and the technology has been used to prepare libraries of peptides, h...

journal_title：Current opinion in chemical biology

authors： Zambaldo C,Barluenga S,Winssinger N

更新日期：2015-06-01 00:00:00

abstract：:Histone proteins and their accompanying post-translational modifications have received much attention for their ability to affect chromatin structure and, hence, regulate gene expression. Recently, mass spectrometry has become an important complementary tool for the analysis of histone variants and modification sites,...

journal_title：Current opinion in chemical biology

authors： Garcia BA,Shabanowitz J,Hunt DF

更新日期：2007-02-01 00:00:00

abstract：:Immunoassays are indispensable for research and clinical analysis, and following the emergence of the omics paradigm, multiplexing of immunoassays is more needed than ever. Cross-reactivity (CR) in multiplexed immunoassays has been unexpectedly difficult to mitigate, preventing scaling up of multiplexing, limiting ass...

journal_title：Current opinion in chemical biology

authors： Juncker D,Bergeron S,Laforte V,Li H

更新日期：2014-02-01 00:00:00

abstract：:A number of nitrile-related enzymes have been screened over the past year for use in synthetic applications. There have also been significant advances in our understanding of the structures and modes of regulation of metal-containing nitrile hydratases. Enzyme structural characterization has provided new insights into...

journal_title：Current opinion in chemical biology

authors： Kobayashi M,Shimizu S

更新日期：2000-02-01 00:00:00

abstract：:Manganese, iron, copper, and zinc are observed to play essential roles in mitochondria. The overload and depletion of metal ions in mitochondria under pathological conditions, however, could disturb mitochondrial compartments and functions leading to cell death. In this review, we mainly summarize how impaired metal i...

journal_title：Current opinion in chemical biology

authors： Nam E,Han J,Suh JM,Yi Y,Lim MH

更新日期：2018-04-01 00:00:00

abstract：:Natural products continue to provide privileged scaffolds for drug discovery. However, challenges in supply and structure diversification can limit development. Here, we discuss recent (2017-2020) examples of synthetic biology approaches used to address challenges in supply and contribute to structure diversification ...

journal_title：Current opinion in chemical biology

authors： Romanowski S,Eustáquio AS

更新日期：2020-10-01 00:00:00

abstract：:During 1996 and 1997, several chemical issues that arise in the early stages of preinitiation complex (PIC) formation were resolved. Kinetics experiments indicated that both TBP dimerization and DNA bending influence the rate of TBP-TATA box assembly. Affinity cleavage experiments indicated that TBP lacks the specific...

journal_title：Current opinion in chemical biology

authors： Cox JM,Kays AR,Sanchez JF,Schepartz A

更新日期：1998-02-01 00:00:00

abstract：:Recent advances in multidimensional nuclear magnetic resonance methodology to obtain 1H, 15N and 13C resonance assignments, interproton distance and torsion angle restraints, and restraints that characterize long-range order, coupled with new methods of structure refinement and novel methods for reducing linewidths, h...

journal_title：Current opinion in chemical biology

authors： Clore GM,Gronenborn AM

更新日期：1998-10-01 00:00:00

abstract：:Within this review article we describe recent developments in asymmetric catalysis with peptides. Numerous peptides have been established in the past two decades that catalyze a wide variety of transformations with high stereoselectivities and yields, as well as broad substrate scope. We highlight here catalytically a...

journal_title：Current opinion in chemical biology

authors： Lewandowski B,Wennemers H

更新日期：2014-10-01 00:00:00

abstract：:Polyketides are a large class of structurally diverse, biologically active natural products. Recent experiments add evidence that many of the enzymes involved in the biosynthesis of these natural products are intrinsically tolerant of nonnatural substrates. In addition, an increasing understanding of structure-functio...

journal_title：Current opinion in chemical biology

authors： Pohl NL

更新日期：2002-12-01 00:00:00