Preinitation complex assembly: potentially a bumpy path.

abstract：:The range and diversity of solid-phase synthetic methodology continues to develop very rapidly, allowing the generation of previously inaccessible compound libraries. The design of such libraries requires consideration of linkage strategies, newly developed solid-phase reactions and emerging solution-phase processes. ...

journal_title：Current opinion in chemical biology

authors： Oliver SF,Abell C

更新日期：1999-06-01 00:00:00

abstract：:Nanoparticle-based therapeutics have received increasing attention, as these systems can alleviate many drawbacks of conventional therapy. Metal-organic frameworks (MOFs), a new class of hybrid materials composed of metal ions and organic bridging ligands, have emerged as a promising platform for drug delivery, owing ...

journal_title：Current opinion in chemical biology

authors： Huxford RC,Della Rocca J,Lin W

更新日期：2010-04-01 00:00:00

abstract：:The micro-environment in which stem cells reside regulates their fate, and synthetic materials have recently been designed to emulate these regulatory processes for various medical applications. Ligands inspired by the natural extracellular matrix, cell-cell contacts, and growth factors have been incorporated into syn...

journal_title：Current opinion in chemical biology

authors： Saha K,Pollock JF,Schaffer DV,Healy KE

更新日期：2007-08-01 00:00:00

abstract：:The eukaryotic ribonucleoprotein reverse transcriptase (RT) telomerase uses a template within its integral RNA subunit to extend chromosome ends by synthesis of single-stranded telomeric repeats. Telomerase is adapted to its unique cellular role by an ability to release product DNA in single-stranded form, regeneratin...

journal_title：Current opinion in chemical biology

authors： Collins K

更新日期：2011-10-01 00:00:00

abstract：:Polyketides are a large class of structurally diverse, biologically active natural products. Recent experiments add evidence that many of the enzymes involved in the biosynthesis of these natural products are intrinsically tolerant of nonnatural substrates. In addition, an increasing understanding of structure-functio...

journal_title：Current opinion in chemical biology

authors： Pohl NL

更新日期：2002-12-01 00:00:00

abstract：:The asymmetric bioreduction of alkenes bearing an electron-withdrawing group using flavin-dependent enzymes from the 'old yellow enzyme' family at the expense of NAD(P)H yields the corresponding non-racemic alkanes going in hand with the creation of up to two chiral carbon centres. To avoid external cofactor recycling...

journal_title：Current opinion in chemical biology

authors： Stuermer R,Hauer B,Hall M,Faber K

更新日期：2007-04-01 00:00:00

abstract：:Oligosaccharide recognition is a major means of bacterial-host cell attachment. Bacterial-host receptor binding can subvert host signaling pathways to cause pathology. In addition, pathogenic bacteria can utilize more than one recognition system to bind host cells. Recent studies of Helicobacter pylori illustrate both...

journal_title：Current opinion in chemical biology

authors： Lingwood CA

更新日期：1998-12-01 00:00:00

abstract：:In recent years, ogliopeptides have enjoyed ever increasing interest in two areas: first, approaches to biomimetic enzyme-like activity; and second, as metal-free catalysts for enantioselective transformations of synthetic interest. The discovery and optimization of peptides for these purposes has often used the metho...

journal_title：Current opinion in chemical biology

authors： Berkessel A

更新日期：2003-06-01 00:00:00

abstract：:The field of computational protein design is reaching its adolescence. Protein design algorithms have been applied to design or engineer proteins that fold, fold faster, catalyze, catalyze faster, signal, and adopt preferred conformational states. Further developments of scoring functions, sampling strategies, and opt...

journal_title：Current opinion in chemical biology

authors： Kraemer-Pecore CM,Wollacott AM,Desjarlais JR

更新日期：2001-12-01 00:00:00

abstract：:Technological advances in the automation of parallel synthesis are following the model set by the semiconductor industry: miniaturization, increasing speed, lower costs. Recent work includes preparation of high-density reaction blocks, development of ink-jet dispensing to polypropylene sheets and synthesis inside cust...

journal_title：Current opinion in chemical biology

authors： Cargill JF,Lebl M

更新日期：1997-06-01 00:00:00

abstract：:Systematic studies of the organization of biochemical networks that make up the living cell can be defined by studying the organization and dynamics of protein interaction networks (PINs). Here, we describe recent conceptual and experimental advances that can achieve this aim and how chemical perturbations of interact...

journal_title：Current opinion in chemical biology

authors： Campbell-Valois FX,Michnick S

更新日期：2005-02-01 00:00:00

abstract：:The biosynthetic pathways of plant natural products offer an abundance of knowledge to scientists in many fields. Synthetic chemists can be inspired by the synthetic strategies that nature uses to construct these compounds. Chemical and biological engineers are working to reprogram these biosynthetic pathways to more ...

journal_title：Current opinion in chemical biology

authors： Usera AR,O'Connor SE

更新日期：2009-10-01 00:00:00

abstract：:The chemokines are a large superfamily of chemotactic cytokines that are utilized to direct the trafficking and migration of leukocytes within the immune system. The chemokines mediate their activity through a large family of G-protein-coupled receptors, and thus are highly tractable as therapeutic targets. Exciting a...

journal_title：Current opinion in chemical biology

authors： Cascieri MA,Springer MS

更新日期：2000-08-01 00:00:00

abstract：:Oligosaccharyl transferase is part of the macromolecular machinery that processes nascent proteins in the endoplasmic reticulum. The enzyme is highly conserved, catalyzes the initial step in the biosynthesis of N-linked glycoproteins and acts as a 'gatekeeper' for the secretory pathway. As more proteins associated wit...

journal_title：Current opinion in chemical biology

authors： Dempski RE Jr,Imperiali B

更新日期：2002-12-01 00:00:00

abstract：:Although new detection screening methods must still be developed, the actual main limitation in combinatorial chemistry seems to be the diversity of ligands that can be generated in terms of real structural and chemical diversity. Thus, there is a strong interest for the development of different strategies for the par...

journal_title：Current opinion in chemical biology

authors： Lavastre O,Bonnette F,Gallard L

更新日期：2004-06-01 00:00:00

abstract：:Peptide arrays have attracted wide interest as tools for discovering biochemical interactions. Because many protein binding and enzyme activities are directed towards peptides, the preparation of arrays having hundreds to thousands of immobilized peptides offers an unprecedented opportunity for identifying interaction...

journal_title：Current opinion in chemical biology

authors： Min DH,Mrksich M

更新日期：2004-10-01 00:00:00

abstract：:The field of enzymology has long used small-molecule mass spectrometry. However, the direct interrogation of covalent and non-covalent intermediates by large-molecule mass spectrometry of enzymes or large peptide substrates is illuminating an increasingly diverse array of chemistries used in nature. Recent advances no...

journal_title：Current opinion in chemical biology

authors： Kelleher NL,Hicks LM

更新日期：2005-10-01 00:00:00

abstract：:Lysine acetylation has emerged as a key post-translational modification found at many sites throughout the cell. It plays an important role in epigenetic processes, and more generally in the regulation of protein stability and interactions. Acetyl groups are installed by lysine acetyltransferases and removed by lysine...

journal_title：Current opinion in chemical biology

authors： Schiedel M,Conway SJ

更新日期：2018-08-01 00:00:00

abstract：:Chemical manipulation of natural, unengineered proteins is a daunting challenge which tests the limits of reaction design. By combining transition-metal or other catalysts with molecular recognition ideas, it is possible to achieve site-selective protein reactivity without the need for engineered recognition sequences...

journal_title：Current opinion in chemical biology

authors： Ball ZT

更新日期：2015-04-01 00:00:00

abstract：:To transfer to the laboratory, the excellent efficiency shown by enzymes in Nature, biocatalysis, had to mimic several synthetic strategies used by the living organisms. Biosynthetic pathways are examples of tandem catalysis and may be assimilated in the biocatalysis field for the use of isolated multi-enzyme systems ...

journal_title：Current opinion in chemical biology

authors： Oroz-Guinea I,García-Junceda E

更新日期：2013-04-01 00:00:00

abstract：:This review explores the concept of using privileged scaffolds to identify biologically active compounds through building chemical libraries. We hope to accomplish three main objectives: to provide one of the most comprehensive listings of privileged scaffolds; to reveal through four selected examples the present stat...

journal_title：Current opinion in chemical biology

authors： Welsch ME,Snyder SA,Stockwell BR

更新日期：2010-06-01 00:00:00

abstract：:Metallodrugs are extensively used to treat and diagnose distinct disease types. The unique physical-chemical properties of metal ions offer tantalizing opportunities to tailor effective scaffolds for selectively targeting specific biomolecules. Modern experimental techniques have collected a large body of structural d...

journal_title：Current opinion in chemical biology

authors： Janoš P,Spinello A,Magistrato A

更新日期：2020-08-08 00:00:00

abstract：:NMR, already some 50 years old, has long been an invaluable analytical method in industry for verification of chemical synthesis and compound characterisation. The range of molecular information accessible through NMR, however, offers a far larger horizon of applications. Of these, ligand screening by NMR has emerged ...

journal_title：Current opinion in chemical biology

authors： Diercks T,Coles M,Kessler H

更新日期：2001-06-01 00:00:00

abstract：:Metabolomics and lipidomics aim to comprehensively measure the dynamic changes of all metabolites and lipids that are present in biological systems. The use of ion mobility-mass spectrometry (IM-MS) for metabolomics and lipidomics has facilitated the separation and the identification of metabolites and lipids in compl...

journal_title：Current opinion in chemical biology

authors： Zhou Z,Tu J,Zhu ZJ

更新日期：2018-02-01 00:00:00

abstract：:The atomic force microscope (AFM) allows biomolecules to be observed and manipulated under native conditions. It operates in buffer solution, produces molecular images with outstanding signal-to-noise ratio, and addresses single molecules. Progress in sample preparation and instrumentation has led to topographs that r...

journal_title：Current opinion in chemical biology

authors： Frederix PL,Akiyama T,Staufer U,Gerber Ch,Fotiadis D,Müller DJ,Engel A

更新日期：2003-10-01 00:00:00

abstract：:Recent advances in multidimensional nuclear magnetic resonance methodology to obtain 1H, 15N and 13C resonance assignments, interproton distance and torsion angle restraints, and restraints that characterize long-range order, coupled with new methods of structure refinement and novel methods for reducing linewidths, h...

journal_title：Current opinion in chemical biology

authors： Clore GM,Gronenborn AM

更新日期：1998-10-01 00:00:00

abstract：:Bacterial-derived antimicrobial polypeptides enjoy a large degree of structural and chemical diversity. Two well-studied examples of such polypeptides are the lanthionine-containing lantibiotics produced by a variety of Gram-positive bacteria, and their Gram-negative counterparts, the microcins. Both groups are produc...

journal_title：Current opinion in chemical biology

authors： Jack RW,Jung G

更新日期：2000-06-01 00:00:00

abstract：:The translation of nucleic acids into synthetic structures with expanded functional potential has been the subject of considerable research, with applications including small-molecule and polymer evolution, reaction discovery and sensing. Here, we review properties of nucleic-acid-templated synthesis in the context of...

journal_title：Current opinion in chemical biology

authors： Calderone CT,Liu DR

更新日期：2004-12-01 00:00:00

abstract：:The global nitrogen cycle is the process in which different forms of environmental N are interconverted by microorganisms either for assimilation into biomass or in respiratory energy-generating pathways. This short review highlights developments over the last 5 years in our understanding of functionality of nitrogena...

journal_title：Current opinion in chemical biology

authors： Eady RR,Antonyuk SV,Hasnain SS

更新日期：2016-04-01 00:00:00

abstract：:The role of integrin and extracellular matrix proteins in various pathological processes (including angiogenesis, thrombosis, apoptosis and cell migration and proliferation), leading to both acute and chronic disease states (e.g. ocular diseases, metastasis, unstable angina, myocardial infarction, stroke, osteoporosis...

journal_title：Current opinion in chemical biology

authors： Mousa SA

更新日期：2002-08-01 00:00:00