Abstract:
:To transfer to the laboratory, the excellent efficiency shown by enzymes in Nature, biocatalysis, had to mimic several synthetic strategies used by the living organisms. Biosynthetic pathways are examples of tandem catalysis and may be assimilated in the biocatalysis field for the use of isolated multi-enzyme systems in the homogeneous phase. The concurrent action of several enzymes that work sequentially presents extraordinary advantages from the synthetic point of view, since it permits a reversible process to become irreversible, to shift the equilibrium reaction in such a way that enantiopure compounds can be obtained from prochiral or racemic substrates, reduce or eliminate problems due to product inhibition or prevent the shortage of substrates by dilution or degradation in the bulk media, etc. In this review we want to illustrate the developments of recent studies involving in vitro multi-enzyme reactions for the synthesis of different classes of organic compounds.
journal_name
Curr Opin Chem Bioljournal_title
Current opinion in chemical biologyauthors
Oroz-Guinea I,García-Junceda Edoi
10.1016/j.cbpa.2013.02.015subject
Has Abstractpub_date
2013-04-01 00:00:00pages
236-49issue
2eissn
1367-5931issn
1879-0402pii
S1367-5931(13)00030-6journal_volume
17pub_type
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
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更新日期:2004-04-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2013.10.009
更新日期:2013-12-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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abstract::Mass spectrometry has become a choice method for broad-spectrum metabolite analysis in both fundamental and applied research. This can range from comprehensive analysis achieved through time-consuming chromatography to the rapid analysis of a few target metabolites without chromatography. In this review article, we hi...
journal_title:Current opinion in chemical biology
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abstract::In recent years pharmaceutical companies have utilized structure-based drug design and combinatorial library design techniques to speed up their drug discovery efforts. Both approaches are routinely used in the lead discovery and lead optimization stages of the drug discovery process. Fragment-based drug design, a new...
journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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