Nitrile hydrolases.

abstract：:Thrombin is a multifunctional serine protease that plays a primary role in the pathogenic pathway of thrombosis as a consequence of its actions on the two principal components of blood clots, fibrin and activated platelets. Deficiencies in available therapies, heparin and coumadin, have recently led to an intense effo...

journal_title：Current opinion in chemical biology

authors： Ripka WC

更新日期：1997-08-01 00:00:00

abstract：:Although new detection screening methods must still be developed, the actual main limitation in combinatorial chemistry seems to be the diversity of ligands that can be generated in terms of real structural and chemical diversity. Thus, there is a strong interest for the development of different strategies for the par...

journal_title：Current opinion in chemical biology

authors： Lavastre O,Bonnette F,Gallard L

更新日期：2004-06-01 00:00:00

abstract：:A wide assortment of caged compounds, which are species whose biological activity can be unleashed with light, have been synthesized and used to investigate a variety of biological phenomena. In contrast, the construction of caged proteins and their application to biological systems has lagged far behind. Recent advan...

journal_title：Current opinion in chemical biology

authors： Curley K,Lawrence DS

更新日期：1999-02-01 00:00:00

abstract：:Immunoassays are indispensable for research and clinical analysis, and following the emergence of the omics paradigm, multiplexing of immunoassays is more needed than ever. Cross-reactivity (CR) in multiplexed immunoassays has been unexpectedly difficult to mitigate, preventing scaling up of multiplexing, limiting ass...

journal_title：Current opinion in chemical biology

authors： Juncker D,Bergeron S,Laforte V,Li H

更新日期：2014-02-01 00:00:00

abstract：:Significant advances have been made over the past decade on illustrating structural and functional features underneath the selective metal ion recognition by various proteins in nature. These efforts led to fruitful information regarding mechanisms that bacteria employed on the regulation, transportation, and utilizat...

journal_title：Current opinion in chemical biology

authors： Chen PR,He C

更新日期：2008-04-01 00:00:00

abstract：:Numerous tools for Zn2+ sensing in living cells have become available in the past three years. Among them, fluorescence imaging using fluorescent sensor molecules has been the most popular approach. Some of these sensor molecules can be used to visualize Zn2+ in living cells. Some of the biological functions of Zn2+ h...

journal_title：Current opinion in chemical biology

authors： Kikuchi K,Komatsu K,Nagano T

更新日期：2004-04-01 00:00:00

abstract：:Oligosaccharide recognition is a major means of bacterial-host cell attachment. Bacterial-host receptor binding can subvert host signaling pathways to cause pathology. In addition, pathogenic bacteria can utilize more than one recognition system to bind host cells. Recent studies of Helicobacter pylori illustrate both...

journal_title：Current opinion in chemical biology

authors： Lingwood CA

更新日期：1998-12-01 00:00:00

abstract：:The design of dimeric coiled-coils has ultimately led to novel applications, such as self-replicating peptide systems, whereas the structural features of the less common trimeric coiled-coil continue to be elucidated. Novel topologies have been discovered in designed proteins, as exemplified by the right-handed tetram...

journal_title：Current opinion in chemical biology

authors： Micklatcher C,Chmielewski J

更新日期：1999-12-01 00:00:00

abstract：:The nature of the oxidized halogen intermediate in vanadium bromoperoxidase has recently been shown to depend on the nature of the organic substrate. For example, in the presence of indoles, vanadium bromoperoxidase does not release a freely diffusible oxidized halogen intermediate (such as HOBr+/-BR2+/-Br3-). Regiose...

journal_title：Current opinion in chemical biology

authors： Butler A

更新日期：1998-04-01 00:00:00

abstract：:In recent years, ogliopeptides have enjoyed ever increasing interest in two areas: first, approaches to biomimetic enzyme-like activity; and second, as metal-free catalysts for enantioselective transformations of synthetic interest. The discovery and optimization of peptides for these purposes has often used the metho...

journal_title：Current opinion in chemical biology

authors： Berkessel A

更新日期：2003-06-01 00:00:00

abstract：:The broad utility of protein bioconjugates has created a need for new and diverse strategies for site-selective protein modification. In particular, chemical reactions that target alternative amino acid side chains or unnatural functional groups are emerging as a valuable complement to more commonly used lysine- and c...

journal_title：Current opinion in chemical biology

authors： Antos JM,Francis MB

更新日期：2006-06-01 00:00:00

abstract：:Nitrogenase catalyzes the important reactions of N2-reduction, CO-reduction and CO2-reduction at its active cofactor site. Designated the M-cluster, this complex metallocofactor is assembled through the generation of a characteristic 8Fe-core before the insertion of Mo and homocitrate that completes the stoichiometry ...

journal_title：Current opinion in chemical biology

authors： Hu Y,Ribbe MW

更新日期：2016-04-01 00:00:00

abstract：:Recent advances in membrane protein crystallography have greatly increased structural information of channels permeating metal ions. Structural bioinformatics techniques and molecular dynamics calculations are providing structural models of ion channels for which the three-dimensional structure is not known. Most of t...

journal_title：Current opinion in chemical biology

authors： Giorgetti A,Carloni P

更新日期：2003-02-01 00:00:00

abstract：:The eukaryotic ribonucleoprotein reverse transcriptase (RT) telomerase uses a template within its integral RNA subunit to extend chromosome ends by synthesis of single-stranded telomeric repeats. Telomerase is adapted to its unique cellular role by an ability to release product DNA in single-stranded form, regeneratin...

journal_title：Current opinion in chemical biology

authors： Collins K

更新日期：2011-10-01 00:00:00

abstract：:The fermentation of synthesis gas (including carbon monoxide, carbon dioxide, and hydrogen) with anaerobic acetogens is an established biotechnological process that has recently been transferred to a commercial scale. The natural product spectrum of acetogens is natively restricted to acetate, ethanol, and 2,3-butaned...

journal_title：Current opinion in chemical biology

authors： Molitor B,Marcellin E,Angenent LT

更新日期：2017-12-01 00:00:00

abstract：:Polyketides are a large class of structurally diverse, biologically active natural products. Recent experiments add evidence that many of the enzymes involved in the biosynthesis of these natural products are intrinsically tolerant of nonnatural substrates. In addition, an increasing understanding of structure-functio...

journal_title：Current opinion in chemical biology

authors： Pohl NL

更新日期：2002-12-01 00:00:00

abstract：:New insights have been gained into the formation of the di-iron tyrosyl radical cofactor, which is essential for nucleotide reduction in the class I ribonucleotide reductases. Recent advances include Insight into the function of the tyrosyl radical in initiation of nucleotide reduction. ...

journal_title：Current opinion in chemical biology

authors： Stubbe J

更新日期：2003-04-01 00:00:00

abstract：:Protein engineering over the past four years has made rhodopsin-based genetically encoded voltage indicators a leading candidate to achieve the task of reporting action potentials from a population of genetically targeted neurons in vivo. Rational design and large-scale screening efforts have steadily improved the dyn...

journal_title：Current opinion in chemical biology

authors： Gong Y

更新日期：2015-08-01 00:00:00

abstract：:Lysine acetylation has emerged as a key post-translational modification found at many sites throughout the cell. It plays an important role in epigenetic processes, and more generally in the regulation of protein stability and interactions. Acetyl groups are installed by lysine acetyltransferases and removed by lysine...

journal_title：Current opinion in chemical biology

authors： Schiedel M,Conway SJ

更新日期：2018-08-01 00:00:00

abstract：:NMR, already some 50 years old, has long been an invaluable analytical method in industry for verification of chemical synthesis and compound characterisation. The range of molecular information accessible through NMR, however, offers a far larger horizon of applications. Of these, ligand screening by NMR has emerged ...

journal_title：Current opinion in chemical biology

authors： Diercks T,Coles M,Kessler H

更新日期：2001-06-01 00:00:00

abstract：:The past decade has seen rapid growth in the use of diverse compound libraries in classical phenotypic screens to identify modulators of a given process. The subsequent process of identifying the molecular targets of active hits, also called 'target deconvolution', is an essential step for understanding compound mecha...

journal_title：Current opinion in chemical biology

authors： Lee J,Bogyo M

更新日期：2013-02-01 00:00:00

abstract：:Activation of unactivated C-H bonds is an important process in nature and organic synthesis. Nature employs a wide range of metalloproteins to efficiently catalyze such reactions. Quantum chemistry can be used to explore the reactivity of such systems. Atomic level insight into the catalytic mechanisms can be gained t...

journal_title：Current opinion in chemical biology

authors： Ye S,Neese F

更新日期：2009-02-01 00:00:00

abstract：:Antibody-drug conjugates (ADCs) require multiple assays for analytical and bioanalytical characterization due to their heterogeneous and dynamic nature. These assays can help address questions from the drug-loading distribution following conjugation to exposure-response relationships after dosing in vivo. This review ...

journal_title：Current opinion in chemical biology

authors： Alley SC,Anderson KE

更新日期：2013-06-01 00:00:00

abstract：:The appearance of the highly pathogenic avian influenza virus H5N1 highlighted the potential impact of influenza virus on humanity. The emergence of this high profile virus stimulated much research towards a better understanding of the key determinants for successful human-to-human transmission and as such has provide...

journal_title：Current opinion in chemical biology

authors： von Itzstein M

更新日期：2008-02-01 00:00:00

abstract：:New catalysts and reaction conditions have been developed for the dynamic kinetic resolution or deracemisation of racemic mixtures of chiral compounds. Specific functional groups that lend themselves particularly well to this approach include chiral secondary alcohols, alpha-amino acids, amines and carboxylic acids. A...

journal_title：Current opinion in chemical biology

authors： Turner NJ

更新日期：2004-04-01 00:00:00

abstract：:The number of new chemical entities has remained rather constant (averaging 37 per year) in the past decade, despite the multiple-fold increase in the number of compounds that are being made and tested. Chemical space requires novel methods that can handle the increasing number of potentially accessible molecules. Nei...

journal_title：Current opinion in chemical biology

authors： Oprea TI

更新日期：2002-06-01 00:00:00

abstract：:Inflammatory and autoimmune diseases, including rheumatoid arthritis, inflammatory bowel diseases, multiple sclerosis, psoriasis and asthma, provide drug discoverers with a tremendous challenge. The precise causes of these diseases are not known, but our understanding of the molecular and cellular mechanisms associate...

journal_title：Current opinion in chemical biology

authors： Lewis AJ,Manning AM

更新日期：1999-08-01 00:00:00

abstract：:Initiating and terminating free-radical reactionis via hydrogen atom transfer (HAT) is an attractive means of avoiding substrate prefunctionalization. Small molecule catalysts and reagents, however, struggle to execute this fundamental step with useful levels of diastereoselectivity and enantioselectivity. In contrast...

journal_title：Current opinion in chemical biology

authors： Nakano Y,Biegasiewicz KF,Hyster TK

更新日期：2019-04-01 00:00:00

abstract：:Recent advances in multidimensional nuclear magnetic resonance methodology to obtain 1H, 15N and 13C resonance assignments, interproton distance and torsion angle restraints, and restraints that characterize long-range order, coupled with new methods of structure refinement and novel methods for reducing linewidths, h...

journal_title：Current opinion in chemical biology

authors： Clore GM,Gronenborn AM

更新日期：1998-10-01 00:00:00

abstract：:In the past five to ten years, it has become increasingly apparent that the function of Fe-S clusters is not limited to electron transfer, a function implicit in their discovery. We now know that the vulnerability of these structures to oxidative destruction is used by nature in sensing O2, iron, and possibly also nit...

journal_title：Current opinion in chemical biology

authors： Beinert H,Kiley PJ

更新日期：1999-04-01 00:00:00