Abstract:
:Herein we review contemporary synthetic and protein design strategies to stabilize the alpha-helical motif in short peptides and miniature proteins. Advances in organometallic catalyst design, specifically for the olefin metathesis reaction, enable the use of hydrocarbon bridges to either crosslink side chains of specific residues or mimic intramolecular hydrogen bonds with carbon-carbon bonds. The resulting hydrocarbon-stapled and hydrogen bond surrogate alpha-helices provide unique synthetic ligands for targeting biomolecules. In the protein design realm, several classes of miniature proteins that display stable helical domains have been engineered and manipulated with powerful in vitro selection technologies to yield libraries of sequences that retain their helical folds. Rational re-design of these scaffolds provide distinctive reagents for the modulation of protein-protein interactions.
journal_name
Curr Opin Chem Bioljournal_title
Current opinion in chemical biologyauthors
Henchey LK,Jochim AL,Arora PSdoi
10.1016/j.cbpa.2008.08.019subject
Has Abstractpub_date
2008-12-01 00:00:00pages
692-7issue
6eissn
1367-5931issn
1879-0402pii
S1367-5931(08)00125-7journal_volume
12pub_type
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