Abstract:
:Signal amplification in conventional enzyme-based biosensors is not high enough to achieve the ultrasensitive detection of biomolecules. In recent years, signal amplification has been improved by combining enzymatic reactions with redox cycling or employing multienzyme labels per detection probe. Electrochemical-chemical redox cycling and electrochemical-chemical-chemical redox cycling allow ultrasensitive detection simply by including one or two more chemicals in a solution without the use of an additional enzyme and/or electrode. Multiple horseradish peroxidase labels on magnetic bead carriers provide high signal enhancement along with a multiplex detection possibility. In both cases, the detection procedures are the same as those in conventional enzyme-based electrochemical sensors.
journal_name
Curr Opin Chem Bioljournal_title
Current opinion in chemical biologyauthors
Yang Hdoi
10.1016/j.cbpa.2012.03.015subject
Has Abstractpub_date
2012-08-01 00:00:00pages
422-8issue
3-4eissn
1367-5931issn
1879-0402pii
S1367-5931(12)00050-6journal_volume
16pub_type
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journal_title:Current opinion in chemical biology
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journal_title:Current opinion in chemical biology
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