Abstract:
:Chemical imaging plays an increasingly important role in studying heterogeneous biological systems. It combines molecular spectroscopy with high-resolution spatial information to create quantitative images of molecular distributions. Here I summarize recent progress in technical developments and biological applications of a specific chemical imaging technique-stimulated Raman scattering (SRS) microscopy. SRS microscopy allows for a wide range of molecules - both endogenous and exogenous - to be imaged at high spatial and temporal resolution in living cells, tissues, and organisms. I will focus on developments in SRS imaging that enable detection of non-fluorescent molecules and drive new biological applications.
journal_name
Curr Opin Chem Bioljournal_title
Current opinion in chemical biologyauthors
Fu Ddoi
10.1016/j.cbpa.2017.05.002subject
Has Abstractpub_date
2017-08-01 00:00:00pages
24-31eissn
1367-5931issn
1879-0402pii
S1367-5931(16)30195-8journal_volume
39pub_type
杂志文章,评审abstract::Four-electron reduction of dioxygen to water and splitting of water to dioxygen are extremely important processes in the context of attaining clean renewable energy sources. High-valent metal-oxo cores are proposed as reactive intermediates in these vital processes, although they have only been isolated in extremely r...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2015.01.014
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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abstract::The active site of cross-linked enzyme crystals can be further modified by chemical means, yielding a new type of chemically engineered enzyme. In the past year, the characteristics of cross-linked protein crystals as microporous materials have been studied and used as stationary phases in chromatographic separations....
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/s1367-5931(99)80007-6
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2007.12.010
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abstract::RNA editing in kinetoplastids involves post-transcriptional insertion and deletion of uridylates (Us) to produce mature mitochondrial mRNAs with sequences specified by trans acting small guide RNAs. In vitro studies indicate the reaction pathway involves endonucleolytic cleavage of the precursor mRNA at the editing si...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2004.06.010
更新日期:2004-08-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2010.07.005
更新日期:2010-12-01 00:00:00
abstract::The asymmetric bioreduction of alkenes bearing an electron-withdrawing group using flavin-dependent enzymes from the 'old yellow enzyme' family at the expense of NAD(P)H yields the corresponding non-racemic alkanes going in hand with the creation of up to two chiral carbon centres. To avoid external cofactor recycling...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2007.02.025
更新日期:2007-04-01 00:00:00
abstract::Herein we review contemporary synthetic and protein design strategies to stabilize the alpha-helical motif in short peptides and miniature proteins. Advances in organometallic catalyst design, specifically for the olefin metathesis reaction, enable the use of hydrocarbon bridges to either crosslink side chains of spec...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2008.08.019
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/s1367-5931(00)00144-7
更新日期:2000-12-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2014.09.011
更新日期:2014-10-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/s1367-5931(98)80123-3
更新日期:1998-08-01 00:00:00
abstract::Oligonucleotide in vitro selection provides surprisingly specific aptamers to protein targets. Synthetic chemistries for modifying nucleotides have been developed to enhance aptamer binding affinity. The diversity of nucleosides amenable to either triphosphate synthesis or phosphoramidite activation is now sufficientl...
journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/s1367-5931(97)80103-2
更新日期:1997-06-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2006.06.003
更新日期:2006-08-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/s1367-5931(99)00031-9
更新日期:1999-12-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2005.10.009
更新日期:2005-12-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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更新日期:2001-04-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2014.10.021
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2020.01.004
更新日期:2020-04-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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更新日期:2014-08-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2004.04.004
更新日期:2004-06-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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更新日期:2015-08-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2004.12.009
更新日期:2005-02-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2018.03.016
更新日期:2018-08-01 00:00:00
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journal_title:Current opinion in chemical biology
pub_type: 杂志文章,评审
doi:10.1016/j.cbpa.2009.11.009
更新日期:2010-04-01 00:00:00