Quantitative chemical imaging with stimulated Raman scattering microscopy.

Abstract:

:Chemical imaging plays an increasingly important role in studying heterogeneous biological systems. It combines molecular spectroscopy with high-resolution spatial information to create quantitative images of molecular distributions. Here I summarize recent progress in technical developments and biological applications of a specific chemical imaging technique-stimulated Raman scattering (SRS) microscopy. SRS microscopy allows for a wide range of molecules - both endogenous and exogenous - to be imaged at high spatial and temporal resolution in living cells, tissues, and organisms. I will focus on developments in SRS imaging that enable detection of non-fluorescent molecules and drive new biological applications.

journal_name

Curr Opin Chem Biol

authors

Fu D

doi

10.1016/j.cbpa.2017.05.002

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

24-31

eissn

1367-5931

issn

1879-0402

pii

S1367-5931(16)30195-8

journal_volume

39

pub_type

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