Molecular modeling of ion channels: structural predictions.

abstract：:Structural modification of naturally occurring beta-lactams and beta-lactones is a highly effective strategy for generating drugs for treating bacterial infections, cancer, obesity, and hyperlipidemia. These drugs acylate catalytic amino acids (serine, threonine, or cysteine) in enzyme targets such as penicillin-bindi...

journal_title：Current opinion in chemical biology

authors： Kluge AF,Petter RC

更新日期：2010-06-01 00:00:00

abstract：:The active site of cross-linked enzyme crystals can be further modified by chemical means, yielding a new type of chemically engineered enzyme. In the past year, the characteristics of cross-linked protein crystals as microporous materials have been studied and used as stationary phases in chromatographic separations....

journal_title：Current opinion in chemical biology

authors： Häring D,Schreier P

更新日期：1999-02-01 00:00:00

abstract：:The rapid growth of bio-sequence information has resulted in an increasing demand for reliable methods that group proteins. A few databases with curated alignments of protein families have demonstrated that expert-driven repositories can keep up with the data deluge in the genome era. These original resources implicit...

journal_title：Current opinion in chemical biology

authors： Liu J,Rost B

更新日期：2003-02-01 00:00:00

abstract：:Fifty years after the publication of the DNA double helix model by Watson and Crick, new nucleic acid structures keep emerging at an ever-increasing rate. The past three years have brought a flurry of new oligonucleotide structures, including those of a Hoogsteen-paired DNA duplex, Holliday junctions, DNA-drug complex...

journal_title：Current opinion in chemical biology

authors： Egli M

更新日期：2004-12-01 00:00:00

abstract：:Protein cysteine residues are central to redox signaling and to protection against oxidative damage through their interactions with reactive oxygen and nitrogen species, and electrophiles. Although there is considerable evidence for a functional role for cysteine modifications, the identity and physiological significa...

journal_title：Current opinion in chemical biology

authors： Chouchani ET,James AM,Fearnley IM,Lilley KS,Murphy MP

更新日期：2011-02-01 00:00:00

abstract：:In recent years, ogliopeptides have enjoyed ever increasing interest in two areas: first, approaches to biomimetic enzyme-like activity; and second, as metal-free catalysts for enantioselective transformations of synthetic interest. The discovery and optimization of peptides for these purposes has often used the metho...

journal_title：Current opinion in chemical biology

authors： Berkessel A

更新日期：2003-06-01 00:00:00

abstract：:Biosensors use biological elements to detect or quantify an analyte of interest. In bioprocessing, biosensors are employed to monitor key metabolites. There are two main types: fully biological systems or biological recognition coupled with physical/chemical detection. New developments in chemical biosensors include m...

journal_title：Current opinion in chemical biology

authors： Dekker L,Polizzi KM

更新日期：2017-10-01 00:00:00

abstract：:Extracellular electron transfer via filamentous protein appendages called 'microbial nanowires' has long been studied in Geobacter and other bacteria because of their crucial role in globally-important environmental processes and their applications for bioenergy, biofuels, and bioelectronics. Thousands of papers thoug...

journal_title：Current opinion in chemical biology

authors： Yalcin SE,Malvankar NS

更新日期：2020-12-01 00:00:00

abstract：:The nature of the oxidized halogen intermediate in vanadium bromoperoxidase has recently been shown to depend on the nature of the organic substrate. For example, in the presence of indoles, vanadium bromoperoxidase does not release a freely diffusible oxidized halogen intermediate (such as HOBr+/-BR2+/-Br3-). Regiose...

journal_title：Current opinion in chemical biology

authors： Butler A

更新日期：1998-04-01 00:00:00

abstract：:Significant progress in our understanding of the mechanism of fatty acid desaturation has been achieved. The site of initial oxidation has been determined for several membrane-bound desaturases and a common cryptoregiochemical theme has been revealed. The results of several studies, including a detailed analysis of a ...

journal_title：Current opinion in chemical biology

authors： Behrouzian B,Buist PH

更新日期：2002-10-01 00:00:00

abstract：:In recent years, an increasing number of proteins have been discovered which utilize heme cofactors to sense oxygen, carbon monoxide and nitric oxide. The identification and characterization of these proteins are revising our understanding of heme-mediated allostery first established in the early 1960s. Biochemical an...

journal_title：Current opinion in chemical biology

authors： Chan MK

更新日期：2001-04-01 00:00:00

abstract：:NMR, already some 50 years old, has long been an invaluable analytical method in industry for verification of chemical synthesis and compound characterisation. The range of molecular information accessible through NMR, however, offers a far larger horizon of applications. Of these, ligand screening by NMR has emerged ...

journal_title：Current opinion in chemical biology

authors： Diercks T,Coles M,Kessler H

更新日期：2001-06-01 00:00:00

abstract：:Thrombin is a multifunctional serine protease that plays a primary role in the pathogenic pathway of thrombosis as a consequence of its actions on the two principal components of blood clots, fibrin and activated platelets. Deficiencies in available therapies, heparin and coumadin, have recently led to an intense effo...

journal_title：Current opinion in chemical biology

authors： Ripka WC

更新日期：1997-08-01 00:00:00

abstract：:Fourier transform infrared (FTIR) spectroscopy has long been a powerful tool for structural analysis of membrane proteins. However, because of difficulties in resolving contributions from individual residues, most of the derived measurements tend to yield average properties for the system under study. Isotope editing,...

journal_title：Current opinion in chemical biology

authors： Arkin IT

更新日期：2006-10-01 00:00:00

abstract：:Elastin-like polypeptides (ELPs) are artificial polypeptides, derived from Val-Pro-Gly-Xaa-Gly (VPGXG) pentapeptide repeats found in human tropoelastin, that reversibly coacervate above a critical temperature. Genetically encodable ELPs are monodisperse, stimuli responsive, and biocompatible, properties that make them...

journal_title：Current opinion in chemical biology

authors： Chilkoti A,Christensen T,MacKay JA

更新日期：2006-12-01 00:00:00

abstract：:Major advances have been made in our understanding of cytochrome c oxidase owing to continued crystallographic work on important intermediates. This, together with a wealth of data derived from selective mutations and sophisticated spectroscopic probes, has provided significant new insights into oxidase dioxygen chemi...

journal_title：Current opinion in chemical biology

authors： Poulos TL,Li H,Raman CS

更新日期：1999-04-01 00:00:00

abstract：:The appearance of the highly pathogenic avian influenza virus H5N1 highlighted the potential impact of influenza virus on humanity. The emergence of this high profile virus stimulated much research towards a better understanding of the key determinants for successful human-to-human transmission and as such has provide...

journal_title：Current opinion in chemical biology

authors： von Itzstein M

更新日期：2008-02-01 00:00:00

abstract：:Natural products continue to provide privileged scaffolds for drug discovery. However, challenges in supply and structure diversification can limit development. Here, we discuss recent (2017-2020) examples of synthetic biology approaches used to address challenges in supply and contribute to structure diversification ...

journal_title：Current opinion in chemical biology

authors： Romanowski S,Eustáquio AS

更新日期：2020-10-01 00:00:00

abstract：:Self-assembled monolayer (SAM) and fluorescent quantum dots (QDs) share common ground as emerging tools for nanoscale observation of biological interactions. SAMs provide excellent means of controlling the surface characteristics through individually tailored and engineered building blocks. SAMs on various surfaces ha...

journal_title：Current opinion in chemical biology

authors： Shin SK,Yoon HJ,Jung YJ,Park JW

更新日期：2006-10-01 00:00:00

abstract：:The definition of molecular diversity and the development of measures for assessing the similarity or dissimilarity of molecules are central tasks for the design of novel biologically active compounds. Combinatorial chemistry allows the coupling of mathematical optimisation methods that do not require the a priori kno...

journal_title：Current opinion in chemical biology

authors： Weber L

更新日期：1998-06-01 00:00:00

abstract：:RNA editing in kinetoplastids involves post-transcriptional insertion and deletion of uridylates (Us) to produce mature mitochondrial mRNAs with sequences specified by trans acting small guide RNAs. In vitro studies indicate the reaction pathway involves endonucleolytic cleavage of the precursor mRNA at the editing si...

journal_title：Current opinion in chemical biology

authors： Stuart K,Allen TE,Kable ML,Lawson S

更新日期：1997-10-01 00:00:00

abstract：:The field of enzymology has long used small-molecule mass spectrometry. However, the direct interrogation of covalent and non-covalent intermediates by large-molecule mass spectrometry of enzymes or large peptide substrates is illuminating an increasingly diverse array of chemistries used in nature. Recent advances no...

journal_title：Current opinion in chemical biology

authors： Kelleher NL,Hicks LM

更新日期：2005-10-01 00:00:00

abstract：:In many species, double-stranded RNA can specifically and effectively silence genes. This newly discovered biological phenomenon, called RNA interference (RNAi), has practical implications for functional genomics. As shown by two recent reports, RNAi provides a rapid method to test the function of genes in the nematod...

journal_title：Current opinion in chemical biology

authors： Barstead R

更新日期：2001-02-01 00:00:00

abstract：:Recent uses of phage-displayed combinatorial peptide and cDNA libraries have proven invaluable in mapping protein-protein interactions, protein-drug interactions, and the generation of 'molecular therapeutics'. This article reviews some of the findings of the past year and points out some of the pros and cons of phage...

journal_title：Current opinion in chemical biology

authors： Rodi DJ,Makowski L,Kay BK

更新日期：2002-02-01 00:00:00

abstract：:Recent advances in -omic profiling technologies have ushered in an era where we no longer want to merely measure the presence or absence of a biomolecule of interest, but instead hope to understand its function and interactions within larger signaling networks. Here, we review several emerging proteomic technologies c...

journal_title：Current opinion in chemical biology

authors： Huang JX,Coukos JS,Moellering RE

更新日期：2020-02-01 00:00:00

abstract：:Herein we review contemporary synthetic and protein design strategies to stabilize the alpha-helical motif in short peptides and miniature proteins. Advances in organometallic catalyst design, specifically for the olefin metathesis reaction, enable the use of hydrocarbon bridges to either crosslink side chains of spec...

journal_title：Current opinion in chemical biology

authors： Henchey LK,Jochim AL,Arora PS

更新日期：2008-12-01 00:00:00

abstract：:RNA plays an essential cellular role in nearly every aspect of the transmission and expression of genetic information, including regulatory roles that have significance for cellular development. Access to RNA bearing synthetic modifications has allowed biological chemists to probe deep into the inner workings of cellu...

journal_title：Current opinion in chemical biology

authors： Das SR,Fong R,Piccirilli JA

更新日期：2005-12-01 00:00:00

abstract：:Given the correlation between many human diseases and mis-regulated transcription, there is a growing need for molecules that can inhibit or mimic key interactions between transcriptional activators and their binding partners. Because transcriptional activators typically participate in many different protein-protein b...

journal_title：Current opinion in chemical biology

authors： Majmudar CY,Mapp AK

更新日期：2005-10-01 00:00:00

abstract：:Covalent attachment of glycosylphosphatidylinositols (GPIs) to the protein C-terminus is one of the most common posttranslational modifications in eukaryotic cells. In addition to anchoring surface proteins to the cell membrane, GPIs also have many other important biological functions, determined by their unique struc...

journal_title：Current opinion in chemical biology

authors： Yu S,Guo Z,Johnson C,Gu G,Wu Q

更新日期：2013-12-01 00:00:00

abstract：:The manufacture and use of protein microarrays with correctly folded and functional content presents significant challenges. Despite this, the feasibility and utility of such undertakings are now clear, and exciting progress has recently been demonstrated in the areas of content generation, printing strategies and pro...

journal_title：Current opinion in chemical biology

authors： Predki PF

更新日期：2004-02-01 00:00:00