The blind men and the filament: Understanding structures and functions of microbial nanowires.

Abstract:

:Extracellular electron transfer via filamentous protein appendages called 'microbial nanowires' has long been studied in Geobacter and other bacteria because of their crucial role in globally-important environmental processes and their applications for bioenergy, biofuels, and bioelectronics. Thousands of papers thought these nanowires as pili without direct evidence. Here, we summarize recent discoveries that could help resolve two decades of confounding observations. Using cryo-electron microscopy with multimodal functional imaging and a suite of electrical, biochemical, and physiological studies, we find that rather than pili, nanowires are composed of cytochromes OmcS and OmcZ that transport electrons via seamless stacking of hemes over micrometers. We discuss the physiological need for two different nanowires and their potential applications for sensing, synthesis, and energy production.

journal_name

Curr Opin Chem Biol

authors

Yalcin SE,Malvankar NS

doi

10.1016/j.cbpa.2020.08.004

subject

Has Abstract

pub_date

2020-12-01 00:00:00

pages

193-201

eissn

1367-5931

issn

1879-0402

pii

S1367-5931(20)30118-6

journal_volume

59

pub_type

杂志文章,评审
  • Modern methods to produce natural-product libraries.

    abstract::Natural sources offer a wealth of chemically diverse compounds that have been evolutionary preselected to modulate biochemical pathways. Several industrial and academic groups are accessing this source using advanced technology platforms. Methods have been reported to generate large and diverse natural-product librari...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/s1367-5931(02)00338-1

    authors: Abel U,Koch C,Speitling M,Hansske FG

    更新日期:2002-08-01 00:00:00

  • Deconvoluting the biology and druggability of protein lipidation using chemical proteomics.

    abstract::Lipids are indispensable cellular building blocks, and their post-translational attachment to proteins makes them important regulators of many biological processes. Dysfunction of protein lipidation is also implicated in many pathological states, yet its systematic analysis presents significant challenges. Thanks to i...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2020.10.002

    authors: Losada de la Lastra A,Hassan S,Tate EW

    更新日期:2020-11-19 00:00:00

  • All-atom simulations to studying metallodrugs/target interactions.

    abstract::Metallodrugs are extensively used to treat and diagnose distinct disease types. The unique physical-chemical properties of metal ions offer tantalizing opportunities to tailor effective scaffolds for selectively targeting specific biomolecules. Modern experimental techniques have collected a large body of structural d...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2020.07.005

    authors: Janoš P,Spinello A,Magistrato A

    更新日期:2020-08-08 00:00:00

  • Advances in design of protein folds and assemblies.

    abstract::Conceptual and computational advances triggered an explosion of designed protein structures in the recent years. Various protein fold geometries have been robustly designed with atomic accuracy, including protein folds unseen in nature. The same principles and tools have been extended to design multi-chain assemblies....

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2017.06.020

    authors: Ljubetič A,Gradišar H,Jerala R

    更新日期:2017-10-01 00:00:00

  • Epigenetic regulation by endogenous metabolite pharmacology.

    abstract::Altered metabolite levels can drive epigenetic changes critical to development and disease. However, in many cases the specific protein-metabolite interactions that underlie this process remain enigmatic. In this review, we make the case that this fundamental missing information may be discovered by applying the tools...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2019.02.002

    authors: Kulkarni RA,Montgomery DC,Meier JL

    更新日期:2019-08-01 00:00:00

  • Stimulus responsive elastin biopolymers: Applications in medicine and biotechnology.

    abstract::Elastin-like polypeptides (ELPs) are artificial polypeptides, derived from Val-Pro-Gly-Xaa-Gly (VPGXG) pentapeptide repeats found in human tropoelastin, that reversibly coacervate above a critical temperature. Genetically encodable ELPs are monodisperse, stimuli responsive, and biocompatible, properties that make them...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2006.10.010

    authors: Chilkoti A,Christensen T,MacKay JA

    更新日期:2006-12-01 00:00:00

  • Nitrile hydrolases.

    abstract::A number of nitrile-related enzymes have been screened over the past year for use in synthetic applications. There have also been significant advances in our understanding of the structures and modes of regulation of metal-containing nitrile hydratases. Enzyme structural characterization has provided new insights into...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/s1367-5931(99)00058-7

    authors: Kobayashi M,Shimizu S

    更新日期:2000-02-01 00:00:00

  • Structure-directed combinatorial library design.

    abstract::In recent years pharmaceutical companies have utilized structure-based drug design and combinatorial library design techniques to speed up their drug discovery efforts. Both approaches are routinely used in the lead discovery and lead optimization stages of the drug discovery process. Fragment-based drug design, a new...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2008.02.007

    authors: Zhou JZ

    更新日期:2008-06-01 00:00:00

  • Mechanisms employed by cellulase systems to gain access through the complex architecture of lignocellulosic substrates.

    abstract::To improve the deconstruction of biomass, the most abundant terrestrial source of carbon polymers, en route to renewable fuels, chemicals, and materials more knowledge is needed into the mechanistic interplay between thermochemical pretreatment and enzymatic hydrolysis. In this review we highlight recent progress in a...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2015.08.014

    authors: Donohoe BS,Resch MG

    更新日期:2015-12-01 00:00:00

  • Encapsulation of proteins for improved delivery.

    abstract::Two recent advances have enabled the development of clinically useful, injectable, sustained-release protein formulations. The first is a nonaqueous, cryogenic atomization process that encapsulates the protein into microspheres composed of a biodegradable polymer from which the protein is released slowly. The second c...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/s1367-5931(98)80133-6

    authors: Putney SD

    更新日期:1998-08-01 00:00:00

  • Selective recognition of metal ions by metalloregulatory proteins.

    abstract::Significant advances have been made over the past decade on illustrating structural and functional features underneath the selective metal ion recognition by various proteins in nature. These efforts led to fruitful information regarding mechanisms that bacteria employed on the regulation, transportation, and utilizat...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2007.12.010

    authors: Chen PR,He C

    更新日期:2008-04-01 00:00:00

  • Systems modeling: a pathway to drug discovery.

    abstract::Systems modeling is emerging as a valuable tool in therapeutics. This is seen by the increasing use of clinically relevant computational models and a rise in systems biology companies working with the pharmaceutical industry. Systems models have helped understand the effects of pharmacological intervention at receptor...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2005.06.008

    authors: Rajasethupathy P,Vayttaden SJ,Bhalla US

    更新日期:2005-08-01 00:00:00

  • Optimizing non-natural protein function with directed evolution.

    abstract::Developing technologies such as unnatural amino acid mutagenesis, non-natural cofactor engineering, and computational design are generating proteins with novel functions; these proteins, however, often do not reach performance targets and would benefit from further optimization. Evolutionary methods can complement the...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2010.11.020

    authors: Brustad EM,Arnold FH

    更新日期:2011-04-01 00:00:00

  • Peroxynitrite: reactive, invasive and enigmatic.

    abstract::The reactive oxygen and nitrogen species superoxide ion, nitric oxide, nitrogen dioxide and peroxynitrite ion all react with biological target molecules. Some of these interactions are carefully orchestrated segments of signal transduction cascades or part of the armamentarium of the immune system, others are patholog...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/S1367-5931(99)80036-2

    authors: Groves JT

    更新日期:1999-04-01 00:00:00

  • NMR structure determination of proteins and protein complexes larger than 20 kDa.

    abstract::Recent advances in multidimensional nuclear magnetic resonance methodology to obtain 1H, 15N and 13C resonance assignments, interproton distance and torsion angle restraints, and restraints that characterize long-range order, coupled with new methods of structure refinement and novel methods for reducing linewidths, h...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/s1367-5931(98)80084-7

    authors: Clore GM,Gronenborn AM

    更新日期:1998-10-01 00:00:00

  • Synthetic in vitro circuits.

    abstract::Inspired by advances in the ability to construct programmable circuits in living organisms, in vitro circuits are emerging as a viable platform for designing, understanding, and exploiting dynamic biochemical circuitry. In vitro systems allow researchers to directly access and manipulate biomolecular parts without the...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2012.05.179

    authors: Hockenberry AJ,Jewett MC

    更新日期:2012-08-01 00:00:00

  • Recent advances in lipid separations and structural elucidation using mass spectrometry combined with ion mobility spectrometry, ion-molecule reactions and fragmentation approaches.

    abstract::Lipids are a vital class of molecules that play important and varied roles in biological processes, however, fully understanding these roles is extremely difficult due to the immense number and diversity of possible lipid species. While recent advances in chromatography and high resolution mass spectrometry have great...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2017.11.009

    authors: Zheng X,Smith RD,Baker ES

    更新日期:2018-02-01 00:00:00

  • Privileged scaffolds for library design and drug discovery.

    abstract::This review explores the concept of using privileged scaffolds to identify biologically active compounds through building chemical libraries. We hope to accomplish three main objectives: to provide one of the most comprehensive listings of privileged scaffolds; to reveal through four selected examples the present stat...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2010.02.018

    authors: Welsch ME,Snyder SA,Stockwell BR

    更新日期:2010-06-01 00:00:00

  • Anti-integrin as novel drug-discovery targets: potential therapeutic and diagnostic implications.

    abstract::The role of integrin and extracellular matrix proteins in various pathological processes (including angiogenesis, thrombosis, apoptosis and cell migration and proliferation), leading to both acute and chronic disease states (e.g. ocular diseases, metastasis, unstable angina, myocardial infarction, stroke, osteoporosis...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/s1367-5931(02)00350-2

    authors: Mousa SA

    更新日期:2002-08-01 00:00:00

  • Overcoming the energetic limitations of syngas fermentation.

    abstract::The fermentation of synthesis gas (including carbon monoxide, carbon dioxide, and hydrogen) with anaerobic acetogens is an established biotechnological process that has recently been transferred to a commercial scale. The natural product spectrum of acetogens is natively restricted to acetate, ethanol, and 2,3-butaned...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2017.10.003

    authors: Molitor B,Marcellin E,Angenent LT

    更新日期:2017-12-01 00:00:00

  • Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases.

    abstract::Methanogenesis from trimethylamine, dimethylamine or monomethylamine is initiated by a series of corrinoid-dependent methyltransferases. The non-homologous genes encoding the full-length methyltransferases each possess an in-frame UAG (amber) codon that does not terminate translation. The amber codon is decoded by a d...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2004.08.012

    authors: Krzycki JA

    更新日期:2004-10-01 00:00:00

  • Mechanistic insights into Diels-Alder reactions in natural product biosynthesis.

    abstract::Natural enzymes that catalyze Diels-Alder reactions have long been sought after, yet few enzymes have been experimentally confirmed to perform this reaction. In the past five years, several stand-alone enzymes that can catalyze the Diels-Alder reaction had been identified and characterized. Among which, the crystal st...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2016.09.015

    authors: Hashimoto T,Kuzuyama T

    更新日期:2016-12-01 00:00:00

  • The computational prediction of toxicity.

    abstract::Recent developments in the prediction of toxicity from chemical structure have been reviewed. Attention has been drawn to some of the problems that can be encountered in the area of predictive toxicology, including the need for a multi-disciplinary approach and the need to address mechanisms of action. Progress has be...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/s1367-5931(00)00218-0

    authors: Barratt MD,Rodford RA

    更新日期:2001-08-01 00:00:00

  • Proteomic approaches to the characterization of protein thiol modification.

    abstract::Protein cysteine residues are central to redox signaling and to protection against oxidative damage through their interactions with reactive oxygen and nitrogen species, and electrophiles. Although there is considerable evidence for a functional role for cysteine modifications, the identity and physiological significa...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2010.11.003

    authors: Chouchani ET,James AM,Fearnley IM,Lilley KS,Murphy MP

    更新日期:2011-02-01 00:00:00

  • Vitamin B12 in the spotlight again.

    abstract::The ability of cobalamin to coordinate different upper axial ligands gives rise to a diversity of reactivity. Traditionally, adenosylcobalamin is associated with radical-based rearrangements, and methylcobalamin with methyl cation transfers. Recently, however, a new role for adenosylcobalamin has been discovered as a ...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2017.01.013

    authors: Bridwell-Rabb J,Drennan CL

    更新日期:2017-04-01 00:00:00

  • Enzyme (re)design: lessons from natural evolution and computation.

    abstract::The (re)design of enzymes to catalyze 'new' reactions is a topic of considerable practical and intellectual interest. Directed evolution (random mutagenesis followed by screening/selection) has been used widely to identify novel biocatalysts. However, 'rational' approaches using either natural divergent evolution or c...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2009.01.014

    authors: Gerlt JA,Babbitt PC

    更新日期:2009-02-01 00:00:00

  • Protein sequence databases.

    abstract::A variety of protein sequence databases exist, ranging from simple sequence repositories, which store data with little or no manual intervention in the creation of the records, to expertly curated universal databases that cover all species and in which the original sequence data are enhanced by the manual addition of ...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2003.12.004

    authors: Apweiler R,Bairoch A,Wu CH

    更新日期:2004-02-01 00:00:00

  • Target deconvolution techniques in modern phenotypic profiling.

    abstract::The past decade has seen rapid growth in the use of diverse compound libraries in classical phenotypic screens to identify modulators of a given process. The subsequent process of identifying the molecular targets of active hits, also called 'target deconvolution', is an essential step for understanding compound mecha...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2012.12.022

    authors: Lee J,Bogyo M

    更新日期:2013-02-01 00:00:00

  • Insights into the physiology of ammonia-oxidizing microorganisms.

    abstract::Nitrification is the aerobic process of the nitrogen cycle that converts ammonia to nitrate and is facilitated by ammonia-oxidizing and nitrite-oxidizing microorganisms. Ammonia-oxidizers are unique chemolithotrophs that evolved specialized networks of electron carriers to generate proton motive force using ammonia as...

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/j.cbpa.2018.09.003

    authors: Stein LY

    更新日期:2019-04-01 00:00:00

  • Cross-linked enzyme crystals.

    abstract::The active site of cross-linked enzyme crystals can be further modified by chemical means, yielding a new type of chemically engineered enzyme. In the past year, the characteristics of cross-linked protein crystals as microporous materials have been studied and used as stationary phases in chromatographic separations....

    journal_title:Current opinion in chemical biology

    pub_type: 杂志文章,评审

    doi:10.1016/s1367-5931(99)80007-6

    authors: Häring D,Schreier P

    更新日期:1999-02-01 00:00:00