Selective recognition of metal ions by metalloregulatory proteins.

abstract：:Recent uses of phage-displayed combinatorial peptide and cDNA libraries have proven invaluable in mapping protein-protein interactions, protein-drug interactions, and the generation of 'molecular therapeutics'. This article reviews some of the findings of the past year and points out some of the pros and cons of phage...

journal_title：Current opinion in chemical biology

authors： Rodi DJ,Makowski L,Kay BK

更新日期：2002-02-01 00:00:00

abstract：:Lipids are a vital class of molecules that play important and varied roles in biological processes, however, fully understanding these roles is extremely difficult due to the immense number and diversity of possible lipid species. While recent advances in chromatography and high resolution mass spectrometry have great...

journal_title：Current opinion in chemical biology

authors： Zheng X,Smith RD,Baker ES

更新日期：2018-02-01 00:00:00

abstract：:Synthetic biologists typically construct new pathways within existing cells. While useful, this approach in many ways ignores the undefined but necessary components of life. A growing number of laboratories have begun to try to remove some of the mysteries of cellular life by building life-like systems from non-living...

journal_title：Current opinion in chemical biology

authors： Forlin M,Lentini R,Mansy SS

更新日期：2012-12-01 00:00:00

abstract：:RNA editing in kinetoplastids involves post-transcriptional insertion and deletion of uridylates (Us) to produce mature mitochondrial mRNAs with sequences specified by trans acting small guide RNAs. In vitro studies indicate the reaction pathway involves endonucleolytic cleavage of the precursor mRNA at the editing si...

journal_title：Current opinion in chemical biology

authors： Stuart K,Allen TE,Kable ML,Lawson S

更新日期：1997-10-01 00:00:00

abstract：:A long-standing goal of molecular imaging is to visualize cellular function within the context of living animals, necessitating the development of reporter genes compatible with deeply penetrant imaging modalities such as ultrasound and magnetic resonance imaging (MRI). Until recently, no reporter genes for ultrasound...

journal_title：Current opinion in chemical biology

authors： Lu GJ,Farhadi A,Mukherjee A,Shapiro MG

更新日期：2018-08-01 00:00:00

abstract：:Given the correlation between many human diseases and mis-regulated transcription, there is a growing need for molecules that can inhibit or mimic key interactions between transcriptional activators and their binding partners. Because transcriptional activators typically participate in many different protein-protein b...

journal_title：Current opinion in chemical biology

authors： Majmudar CY,Mapp AK

更新日期：2005-10-01 00:00:00

abstract：:Nanoparticle-based therapeutics have received increasing attention, as these systems can alleviate many drawbacks of conventional therapy. Metal-organic frameworks (MOFs), a new class of hybrid materials composed of metal ions and organic bridging ligands, have emerged as a promising platform for drug delivery, owing ...

journal_title：Current opinion in chemical biology

authors： Huxford RC,Della Rocca J,Lin W

更新日期：2010-04-01 00:00:00

abstract：:Structural genomics (SG) has significantly increased the number of novel protein structures of targets with medical relevance. In the protein kinase area, SG has contributed >50% of all novel kinases structures during the past three years and determined more than 30 novel catalytic domain structures. Many of the relea...

journal_title：Current opinion in chemical biology

authors： Marsden BD,Knapp S

更新日期：2008-02-01 00:00:00

abstract：:To transfer to the laboratory, the excellent efficiency shown by enzymes in Nature, biocatalysis, had to mimic several synthetic strategies used by the living organisms. Biosynthetic pathways are examples of tandem catalysis and may be assimilated in the biocatalysis field for the use of isolated multi-enzyme systems ...

journal_title：Current opinion in chemical biology

authors： Oroz-Guinea I,García-Junceda E

更新日期：2013-04-01 00:00:00

abstract：:Developing technologies such as unnatural amino acid mutagenesis, non-natural cofactor engineering, and computational design are generating proteins with novel functions; these proteins, however, often do not reach performance targets and would benefit from further optimization. Evolutionary methods can complement the...

journal_title：Current opinion in chemical biology

authors： Brustad EM,Arnold FH

更新日期：2011-04-01 00:00:00

abstract：:Although new detection screening methods must still be developed, the actual main limitation in combinatorial chemistry seems to be the diversity of ligands that can be generated in terms of real structural and chemical diversity. Thus, there is a strong interest for the development of different strategies for the par...

journal_title：Current opinion in chemical biology

authors： Lavastre O,Bonnette F,Gallard L

更新日期：2004-06-01 00:00:00

abstract：:The global nitrogen cycle is the process in which different forms of environmental N are interconverted by microorganisms either for assimilation into biomass or in respiratory energy-generating pathways. This short review highlights developments over the last 5 years in our understanding of functionality of nitrogena...

journal_title：Current opinion in chemical biology

authors： Eady RR,Antonyuk SV,Hasnain SS

更新日期：2016-04-01 00:00:00

abstract：:Nitrification is the aerobic process of the nitrogen cycle that converts ammonia to nitrate and is facilitated by ammonia-oxidizing and nitrite-oxidizing microorganisms. Ammonia-oxidizers are unique chemolithotrophs that evolved specialized networks of electron carriers to generate proton motive force using ammonia as...

journal_title：Current opinion in chemical biology

authors： Stein LY

更新日期：2019-04-01 00:00:00

abstract：:The fermentation of synthesis gas (including carbon monoxide, carbon dioxide, and hydrogen) with anaerobic acetogens is an established biotechnological process that has recently been transferred to a commercial scale. The natural product spectrum of acetogens is natively restricted to acetate, ethanol, and 2,3-butaned...

journal_title：Current opinion in chemical biology

authors： Molitor B,Marcellin E,Angenent LT

更新日期：2017-12-01 00:00:00

abstract：:Combinatorial chemistry methods and high-throughput screening for leads in industrial drug discovery have generated a potential bottleneck in the optimisation processes that seek to align potency with good pharmacokinetics in order to produce good medicines. This has resulted in the need for higher throughput methods ...

journal_title：Current opinion in chemical biology

authors： Tarbit MH,Berman J

更新日期：1998-06-01 00:00:00

abstract：:Although biotin and lipoic acid are two universally conserved cofactors essential for intermediary metabolism, their synthetic pathways have become known only in recent years. Both pathways have unusual features. Biotin synthesis in Escherichia coli requires a methylation that is later removed whereas lipoic acid is a...

journal_title：Current opinion in chemical biology

authors： Cronan JE

更新日期：2018-12-01 00:00:00

abstract：:There are likely more than 500000 potential phosphorylation sites in a cellular proteome. This dynamic phosphorylation is under tight control of a variety of kinases and phosphatases. In recent years significant progress has been made in the large-scale analysis of these in vitro and in vivo protein phosphorylation ev...

journal_title：Current opinion in chemical biology

authors： Lemeer S,Heck AJ

更新日期：2009-10-01 00:00:00

abstract：:Natural products containing a gamma-butyrolactone ring are abundant in nature; however, few general synthetic approaches to their stereoselective synthesis with broad structural variety are known. In this article, recent developments towards mono- and polycyclic gamma-butyrolactone natural products and analogs are dis...

journal_title：Current opinion in chemical biology

authors： Seitz M,Reiser O

更新日期：2005-06-01 00:00:00

abstract：:The past decade has seen rapid growth in the use of diverse compound libraries in classical phenotypic screens to identify modulators of a given process. The subsequent process of identifying the molecular targets of active hits, also called 'target deconvolution', is an essential step for understanding compound mecha...

journal_title：Current opinion in chemical biology

authors： Lee J,Bogyo M

更新日期：2013-02-01 00:00:00

abstract：:The asymmetric bioreduction of alkenes bearing an electron-withdrawing group using flavin-dependent enzymes from the 'old yellow enzyme' family at the expense of NAD(P)H yields the corresponding non-racemic alkanes going in hand with the creation of up to two chiral carbon centres. To avoid external cofactor recycling...

journal_title：Current opinion in chemical biology

authors： Stuermer R,Hauer B,Hall M,Faber K

更新日期：2007-04-01 00:00:00

abstract：:Initiating and terminating free-radical reactionis via hydrogen atom transfer (HAT) is an attractive means of avoiding substrate prefunctionalization. Small molecule catalysts and reagents, however, struggle to execute this fundamental step with useful levels of diastereoselectivity and enantioselectivity. In contrast...

journal_title：Current opinion in chemical biology

authors： Nakano Y,Biegasiewicz KF,Hyster TK

更新日期：2019-04-01 00:00:00

abstract：:Electron bifurcation, or the coupling of exergonic and endergonic oxidation-reduction reactions, was discovered by Peter Mitchell and provides an elegant mechanism to rationalize and understand the logic that underpins the Q cycle of the respiratory chain. Thought to be a unique reaction of respiratory complex III for...

journal_title：Current opinion in chemical biology

authors： Peters JW,Beratan DN,Bothner B,Dyer RB,Harwood CS,Heiden ZM,Hille R,Jones AK,King PW,Lu Y,Lubner CE,Minteer SD,Mulder DW,Raugei S,Schut GJ,Seefeldt LC,Tokmina-Lukaszewska M,Zadvornyy OA,Zhang P,Adams MW

更新日期：2018-12-01 00:00:00

abstract：:The range and diversity of solid-phase synthetic methodology continues to develop very rapidly, allowing the generation of previously inaccessible compound libraries. The design of such libraries requires consideration of linkage strategies, newly developed solid-phase reactions and emerging solution-phase processes. ...

journal_title：Current opinion in chemical biology

authors： Oliver SF,Abell C

更新日期：1999-06-01 00:00:00

abstract：:Major advances have been made in our understanding of cytochrome c oxidase owing to continued crystallographic work on important intermediates. This, together with a wealth of data derived from selective mutations and sophisticated spectroscopic probes, has provided significant new insights into oxidase dioxygen chemi...

journal_title：Current opinion in chemical biology

authors： Poulos TL,Li H,Raman CS

更新日期：1999-04-01 00:00:00

abstract：:Molecular oxygen plays an indispensable role as a terminal electron acceptor in the electron transport chain in mitochondria. Acute or chronic oxygen deprivation (hypoxia) in organisms results in various diseases, and the elucidation of the pathogenic mechanism of hypoxia-related diseases and various cellular response...

journal_title：Current opinion in chemical biology

authors： Tobita S,Yoshihara T

更新日期：2016-08-01 00:00:00

abstract：:A variety of protein sequence databases exist, ranging from simple sequence repositories, which store data with little or no manual intervention in the creation of the records, to expertly curated universal databases that cover all species and in which the original sequence data are enhanced by the manual addition of ...

journal_title：Current opinion in chemical biology

authors： Apweiler R,Bairoch A,Wu CH

更新日期：2004-02-01 00:00:00

abstract：:Fragment-based screening has now become an established method for the generation of lead molecules against therapeutic targets. Fragment molecules are simple, low molecular-weight compounds with few chemical functionalities. These characteristics lead to high hit rates for fragment screening as compared to the more cl...

journal_title：Current opinion in chemical biology

authors： Barelier S,Krimm I

更新日期：2011-08-01 00:00:00

abstract：:Mass spectrometry has become a choice method for broad-spectrum metabolite analysis in both fundamental and applied research. This can range from comprehensive analysis achieved through time-consuming chromatography to the rapid analysis of a few target metabolites without chromatography. In this review article, we hi...

journal_title：Current opinion in chemical biology

authors： de Raad M,Fischer CR,Northen TR

更新日期：2016-02-01 00:00:00

abstract：:Biosensors use biological elements to detect or quantify an analyte of interest. In bioprocessing, biosensors are employed to monitor key metabolites. There are two main types: fully biological systems or biological recognition coupled with physical/chemical detection. New developments in chemical biosensors include m...

journal_title：Current opinion in chemical biology

authors： Dekker L,Polizzi KM

更新日期：2017-10-01 00:00:00

abstract：:Majority of eukaryotic proteins are glycosylated and their glycan moieties have numerous important structural, functional and regulatory roles. Because of structural complexity of glycans and technological limitations glycomics, and particularly glycoproteomics was not able to follow rapid progress in genomics and pro...

journal_title：Current opinion in chemical biology

authors： Zoldoš V,Horvat T,Lauc G

更新日期：2013-02-01 00:00:00