The computational prediction of toxicity.

abstract：:Over the past two decades, there has been intense interest in designing and implementing selective (bioorthogonal) reactions for biomolecule tracking. Here we review the most widely used bioorthogonal chemistries in live cells and animals, drawing particular attention to the unique functional groups underlying these t...

journal_title：Current opinion in chemical biology

authors： Shih HW,Kamber DN,Prescher JA

更新日期：2014-08-01 00:00:00

abstract：:The first photocaged complexes appeared in the mid-1980s specifically to complement the ongoing work studying intracellular Ca(2+) signaling with fluorescent probes. Recently, the field of biological inorganic chemistry has focused increasingly on the homeostasis mechanisms and possible signaling functions of transiti...

journal_title：Current opinion in chemical biology

authors： Gwizdala C,Burdette SC

更新日期：2013-04-01 00:00:00

abstract：:The manufacture and use of protein microarrays with correctly folded and functional content presents significant challenges. Despite this, the feasibility and utility of such undertakings are now clear, and exciting progress has recently been demonstrated in the areas of content generation, printing strategies and pro...

journal_title：Current opinion in chemical biology

authors： Predki PF

更新日期：2004-02-01 00:00:00

abstract：:Recent uses of phage-displayed combinatorial peptide and cDNA libraries have proven invaluable in mapping protein-protein interactions, protein-drug interactions, and the generation of 'molecular therapeutics'. This article reviews some of the findings of the past year and points out some of the pros and cons of phage...

journal_title：Current opinion in chemical biology

authors： Rodi DJ,Makowski L,Kay BK

更新日期：2002-02-01 00:00:00

abstract：:Peptides that recognize specific cell types promise to be valuable tools both in research and clinical applications. Cell-specific peptides can be useful as drug delivery vehicles, diagnostic agents, affinity reagents for cell purification, gene therapy delivery agents, and research tools to probe the nature of a cell...

journal_title：Current opinion in chemical biology

authors： Brown KC

更新日期：2000-02-01 00:00:00

abstract：:Phosphorus cycling in the biosphere has traditionally been thought to involve almost exclusively transformations of the element in its pentavalent oxidation state. Recent evidence, however, suggests that a significant fraction of environmental phosphorus may exist in a more reduced form. Most abundant of these reduced...

journal_title：Current opinion in chemical biology

authors： Chin JP,McGrath JW,Quinn JP

更新日期：2016-04-01 00:00:00

abstract：:Study of protein adsorption to solid surfaces continues to be substantial because of its role in cellular responses to biomaterials, interest in molecular aspects such as conformation and orientation, new methods for making protein repellent surfaces, and new application areas such as nanoparticles and microfluidics. ...

journal_title：Current opinion in chemical biology

authors： Szott LM,Horbett TA

更新日期：2011-10-01 00:00:00

abstract：:A broad range of biochemicals, from proteins to nucleic acids, function properly only when associated with a metal, usually a divalent cation. Not any divalent metal will do: these metals differ in their ionic radius, dissociation in water, ionization potential, and number of unpaired electrons in their outer shells, ...

journal_title：Current opinion in chemical biology

authors： Bloom AJ

更新日期：2019-04-01 00:00:00

abstract：:Chromatin organization spans a wide range of structural complexity. Substructures at the 10-200nm scale are poorly characterized, especially in living cells, due to the limitations of electron microscopy and standard optical microscopy. Recently developed super-resolution fluorescence microscopy methods represent an e...

journal_title：Current opinion in chemical biology

authors： Flors C,Earnshaw WC

更新日期：2011-12-01 00:00:00

abstract：:Elastin-like polypeptides (ELPs) are artificial polypeptides, derived from Val-Pro-Gly-Xaa-Gly (VPGXG) pentapeptide repeats found in human tropoelastin, that reversibly coacervate above a critical temperature. Genetically encodable ELPs are monodisperse, stimuli responsive, and biocompatible, properties that make them...

journal_title：Current opinion in chemical biology

authors： Chilkoti A,Christensen T,MacKay JA

更新日期：2006-12-01 00:00:00

abstract：:The asymmetric bioreduction of alkenes bearing an electron-withdrawing group using flavin-dependent enzymes from the 'old yellow enzyme' family at the expense of NAD(P)H yields the corresponding non-racemic alkanes going in hand with the creation of up to two chiral carbon centres. To avoid external cofactor recycling...

journal_title：Current opinion in chemical biology

authors： Stuermer R,Hauer B,Hall M,Faber K

更新日期：2007-04-01 00:00:00

abstract：:Fragment-based screening has now become an established method for the generation of lead molecules against therapeutic targets. Fragment molecules are simple, low molecular-weight compounds with few chemical functionalities. These characteristics lead to high hit rates for fragment screening as compared to the more cl...

journal_title：Current opinion in chemical biology

authors： Barelier S,Krimm I

更新日期：2011-08-01 00:00:00

abstract：:Due to the existence of numerous isomers, the in-depth analysis of glycans represents a major challenge. Currently, the majority of glycans are analysed using mass spectrometry (MS)-based techniques, which can provide information on regioisomers but usually fail to differentiate stereoisomers. A promising approach to ...

journal_title：Current opinion in chemical biology

authors： Manz C,Pagel K

更新日期：2018-02-01 00:00:00

abstract：:Electron bifurcation, or the coupling of exergonic and endergonic oxidation-reduction reactions, was discovered by Peter Mitchell and provides an elegant mechanism to rationalize and understand the logic that underpins the Q cycle of the respiratory chain. Thought to be a unique reaction of respiratory complex III for...

journal_title：Current opinion in chemical biology

authors： Peters JW,Beratan DN,Bothner B,Dyer RB,Harwood CS,Heiden ZM,Hille R,Jones AK,King PW,Lu Y,Lubner CE,Minteer SD,Mulder DW,Raugei S,Schut GJ,Seefeldt LC,Tokmina-Lukaszewska M,Zadvornyy OA,Zhang P,Adams MW

更新日期：2018-12-01 00:00:00

abstract：:Cellular communication drives diverse aspects of organismal biology ranging from immune function to memory formation. The mechanisms by which cells transact information in vivo, though, are not completely understood. This is due, in part, to a lack of tools for observing collections of cells in their native habitats. ...

journal_title：Current opinion in chemical biology

authors： Porterfield WB,Prescher JA

更新日期：2015-02-01 00:00:00

abstract：:Combinatorial chemistry methods and high-throughput screening for leads in industrial drug discovery have generated a potential bottleneck in the optimisation processes that seek to align potency with good pharmacokinetics in order to produce good medicines. This has resulted in the need for higher throughput methods ...

journal_title：Current opinion in chemical biology

authors： Tarbit MH,Berman J

更新日期：1998-06-01 00:00:00

abstract：:A variety of protein sequence databases exist, ranging from simple sequence repositories, which store data with little or no manual intervention in the creation of the records, to expertly curated universal databases that cover all species and in which the original sequence data are enhanced by the manual addition of ...

journal_title：Current opinion in chemical biology

authors： Apweiler R,Bairoch A,Wu CH

更新日期：2004-02-01 00:00:00

abstract：:The metal-free hydrogenase from methanogenic archaea (Hmd) is a unique enzyme: it catalyzes the reaction of its substrate, methenyl-tetrahydromethanopterin, with molecular hydrogen without the aid of a transition metal. In other words, Hmd is currently the only example of a purely organic hydrogenation catalyst. Recen...

journal_title：Current opinion in chemical biology

authors： Berkessel A

更新日期：2001-10-01 00:00:00

abstract：:Recent advances in multidimensional nuclear magnetic resonance methodology to obtain 1H, 15N and 13C resonance assignments, interproton distance and torsion angle restraints, and restraints that characterize long-range order, coupled with new methods of structure refinement and novel methods for reducing linewidths, h...

journal_title：Current opinion in chemical biology

authors： Clore GM,Gronenborn AM

更新日期：1998-10-01 00:00:00

abstract：:Developing technologies such as unnatural amino acid mutagenesis, non-natural cofactor engineering, and computational design are generating proteins with novel functions; these proteins, however, often do not reach performance targets and would benefit from further optimization. Evolutionary methods can complement the...

journal_title：Current opinion in chemical biology

authors： Brustad EM,Arnold FH

更新日期：2011-04-01 00:00:00

abstract：:To improve the deconstruction of biomass, the most abundant terrestrial source of carbon polymers, en route to renewable fuels, chemicals, and materials more knowledge is needed into the mechanistic interplay between thermochemical pretreatment and enzymatic hydrolysis. In this review we highlight recent progress in a...

journal_title：Current opinion in chemical biology

authors： Donohoe BS,Resch MG

更新日期：2015-12-01 00:00:00

abstract：:The number of new chemical entities has remained rather constant (averaging 37 per year) in the past decade, despite the multiple-fold increase in the number of compounds that are being made and tested. Chemical space requires novel methods that can handle the increasing number of potentially accessible molecules. Nei...

journal_title：Current opinion in chemical biology

authors： Oprea TI

更新日期：2002-06-01 00:00:00

abstract：:Although new detection screening methods must still be developed, the actual main limitation in combinatorial chemistry seems to be the diversity of ligands that can be generated in terms of real structural and chemical diversity. Thus, there is a strong interest for the development of different strategies for the par...

journal_title：Current opinion in chemical biology

authors： Lavastre O,Bonnette F,Gallard L

更新日期：2004-06-01 00:00:00

abstract：:Peptide natural products (PNPs) represent a unique class of compounds known for their fascinating structural motifs with important biological activities. Lately, PNPs have garnered a lot of interest for their application in drug discovery. Nevertheless, lack of diversity oriented synthetic/biosynthetic platforms to ge...

journal_title：Current opinion in chemical biology

authors： Maini R,Umemoto S,Suga H

更新日期：2016-10-01 00:00:00

abstract：:Majority of eukaryotic proteins are glycosylated and their glycan moieties have numerous important structural, functional and regulatory roles. Because of structural complexity of glycans and technological limitations glycomics, and particularly glycoproteomics was not able to follow rapid progress in genomics and pro...

journal_title：Current opinion in chemical biology

authors： Zoldoš V,Horvat T,Lauc G

更新日期：2013-02-01 00:00:00

abstract：:Oligosaccharide recognition is a major means of bacterial-host cell attachment. Bacterial-host receptor binding can subvert host signaling pathways to cause pathology. In addition, pathogenic bacteria can utilize more than one recognition system to bind host cells. Recent studies of Helicobacter pylori illustrate both...

journal_title：Current opinion in chemical biology

authors： Lingwood CA

更新日期：1998-12-01 00:00:00

abstract：:Great progress has been made in expanding the repertoire of genetically encoded fluorescent sensors for monitoring intracellular transition metals (TMs). This powerful toolkit permits dynamic and non-invasive detection of TMs with high spatial-temporal resolution, which enables us to better understand the roles of TM ...

journal_title：Current opinion in chemical biology

authors： Hao Z,Zhu R,Chen PR

更新日期：2018-04-01 00:00:00

abstract：:The definition of molecular diversity and the development of measures for assessing the similarity or dissimilarity of molecules are central tasks for the design of novel biologically active compounds. Combinatorial chemistry allows the coupling of mathematical optimisation methods that do not require the a priori kno...

journal_title：Current opinion in chemical biology

authors： Weber L

更新日期：1998-06-01 00:00:00

abstract：:The eukaryotic ribonucleoprotein reverse transcriptase (RT) telomerase uses a template within its integral RNA subunit to extend chromosome ends by synthesis of single-stranded telomeric repeats. Telomerase is adapted to its unique cellular role by an ability to release product DNA in single-stranded form, regeneratin...

journal_title：Current opinion in chemical biology

authors： Collins K

更新日期：2011-10-01 00:00:00

abstract：:Nitrification is the aerobic process of the nitrogen cycle that converts ammonia to nitrate and is facilitated by ammonia-oxidizing and nitrite-oxidizing microorganisms. Ammonia-oxidizers are unique chemolithotrophs that evolved specialized networks of electron carriers to generate proton motive force using ammonia as...

journal_title：Current opinion in chemical biology

authors： Stein LY

更新日期：2019-04-01 00:00:00