Abstract:
:Experiments on spinal cord injury (SCI) have largely focused on the transplantation of stem cells into injured spinal cords for motor recovery while neglecting to investigate bladder dysfunction. The present study was performed to investigate the effect of B10 human mesenchymal stem cells (hMSCs) directly transplanted into the bladder wall of SCI rats and to determine whether they are capable of inhibiting collagen deposition and improving cystometric parameters in SCI rats. Forty 6-week-old female Sprague-Dawley rats were divided into four groups (group 1: control, group 2: sham operated, group 3: SCI, group 4: SCI rats that received B10 cells). B10 cells were labeled with fluorescent magnetic nanoparticles (MNPs). Four weeks after the onset of SCI, MNP-labeled B10 cells were injected to the bladder wall. Serial magnetic resonance (MR) images were taken immediately after MNP-B10 injection and at 4 weeks posttransplantation. Voiding function was assessed at 4 weeks posttransplantation, and the bladder was harvested. Improvements in bladder fibrosis and bladder function were monitored by molecular MR imaging. Transplantation of B10 cells into the SCI rats markedly reduced their weights and collagen deposition. MR images showed a clear hypointense signal induced by the MNP-labeled B10 cells at 4 weeks posttransplantation. Transplanted B10 cells were found to differentiate into smooth muscle cells. The intercontraction interval decreased, and the maximal voiding pressure increased after SCI but recovered after B10 cell transplantation. Survival of B10 cells was found at 4 weeks posttransplantation using anti-human mitochondria antibody staining and MR imaging. The transplanted B10 cells inhibited bladder fibrosis and ameliorated bladder dysfunction in the rat SCI model. MSC-based cell transplantation may be a novel therapeutic strategy for bladder dysfunction in patients with SCI.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Lee HJ,An J,Doo SW,Kim JH,Choi SS,Lee SR,Park SW,Song YS,Kim SUdoi
10.3727/096368914X682125subject
Has Abstractpub_date
2015-01-01 00:00:00pages
1253-63issue
7eissn
0963-6897issn
1555-3892pii
content-CT-1227_Leejournal_volume
24pub_type
杂志文章abstract::The main challenge of cell transplantation as a treatment of myopathies is the large amount of tissue to treat. Intravascular delivery of cells may be an ideal route if proven to be effective and safe. Given the importance of nonhuman primates for preclinical research in transplantation, we tested the intra-arterial i...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X685032
更新日期:2014-01-01 00:00:00
abstract::Biological pacing has been proposed as a physiologic counterpart to electronic pacing, and the sinoatrial node (SAN) is the general standard for biological pacemakers. We tested the expression of SAN pacemaker cell activity when implanted autologously in the right ventricle (RV). We induced complete heart block and i...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X565038
更新日期:2011-01-01 00:00:00
abstract::A large proportion of islets are lost after transplantation partly due to a lack of functional vasculature. Islets revascularize from host tissue but the process takes up to 2 weeks and has been suggested to result in reduced vascular density in engrafted islets. We describe a method for observing and quantifying the ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000007783464993
更新日期:2007-01-01 00:00:00
abstract::Insufficient oxygenation can limit the long-term survival of encapsulated islets in subcutaneous tissue. Transplantation of coencapsulated pig islets with adipose or bone marrow mesenchymal stem cells (AMSCs or BM-MSCs, respectively) was investigated with regard to implant vascularization, oxygenation, and diabetes co...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X663550
更新日期:2014-01-01 00:00:00
abstract::Major skin burns are difficult to treat. Patients often require special care and long-term hospitalization. Besides specific complications associated with the wounds themselves, there may be impairment of the immune system and of other organs. Mesenchymal stromal cells (MSCs) are a recent therapeutic alternative to tr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688128
更新日期:2016-01-01 00:00:00
abstract::Radiotherapy is an effective treatment for brain tumors but often results in cognitive deficits in survivors. Transplantation of embryonic or brain-derived neural stem/progenitor cells (BNSPCs) ameliorated cognitive impairment after irradiation (IR) in animal models. However, such an approach in patients requires a cl...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X674648
更新日期:2014-01-01 00:00:00
abstract::Bisphosphonates (BPs) are widely used to treat several metabolic and oncological diseases affecting the skeletal system. Despite BPs' well-known therapeutic potential, they also displayed important side effects, among which is BPs-related osteonecrosis of the jaw, by targeting osteoclast activities, osteoblast, and os...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720948497
更新日期:2020-01-01 00:00:00
abstract::Testicular germ cell tumors (TGCTs) are highly prevalent in young men aged 20-40 years and are one of the most common lethal solid tumors in men of this age. Due to the current unclear mechanism of tumor development, there is a lack of effective treatment, and therefore in-depth research of the molecular mechanism of ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720946653
更新日期:2020-01-01 00:00:00
abstract::Endothelial progenitor cells (EPCs) have been applied for cell therapy because of their roles in angiogenesis and neovascularization in ischemic tissue. However, adverse responses caused by EPC therapy have not been fully investigated. In this study, a human peripheral blood sample was collected from a healthy donor a...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718821686
更新日期:2019-07-01 00:00:00
abstract::The human neuronal cell-1 (HCN-1) line has recently been established. Under favorable conditions, these cells differentiate into mature neuronal phenotypes. Here we report on further characterization of these cells. Cultured HCN-1 cells express fibronectin immunoreactivity and grow well on fibronectin substrate but do...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979200100104
更新日期:1992-01-01 00:00:00
abstract::This invited concise review was written for the special issue of Cell Transplantation to celebrate the 25th anniversary of the American Society for Neural Therapy and Repair (ASNTR). I aimed to present a succinct summary of two interweaved lines of research work carried out by my team members and collaborators over th...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719850088
更新日期:2019-04-01 00:00:00
abstract::Cardiovascular disease is a major cause of morbidity, disability, and mortality in kidney transplant patients. Cumulative reports indicate that the excessive risk of cardiovascular events is not entirely explained by the increased prevalence of traditional cardiovascular risk factors. Atherosclerosis is a chronic infl...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1177/0963689717735404
更新日期:2017-09-01 00:00:00
abstract::We developed a reliable and quantitative method for measuring the dynamic process of unidirectional two-dimensional (2-D) tissue formation of endothelial cells (ECs) in vitro. The culturing of bovine ECs in an assembled culture chamber provided a square monolayered cell sheet with a linear margin when disassembled at ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979200100601
更新日期:1992-01-01 00:00:00
abstract::Huntington's disease (HD) is a devastating neurodegenerative disorder that occurs in patients with a mutation in the huntingtin or IT15 gene. Patients are plagued by early cognitive signs, motor deficits, and psychiatric disturbances. Symptoms are attributed to cell death in the striatum and disruption of cortical-str...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/000000007783464687
更新日期:2007-01-01 00:00:00
abstract::Spinocerebellar ataxia type 1 (SCA1) is a dominant inherited disease caused by expanded trinucleotide repeats resulting in an increased polyglutamine tract in the gene product. As a potential therapeutic approach for SCA1, we tested antisense RNAs targeting two regions of the ataxin-1 message. Single-stranded regions ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908786516729
更新日期:2008-01-01 00:00:00
abstract::This study examined how the difference in pore size of porous scaffolds affected the in vitro chondrogenic differentiation of seeded adipose stem cells (ASCs) and the in vivo cartilage repair of ASC/scaffold construct. ASCs were isolated from 18 rabbits and seeded in a porous poly (ε-caprolactone) (PCL) scaffold with ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X638865
更新日期:2012-01-01 00:00:00
abstract::Prognosis of patients with heart failure remains poor despite improved conventional and interventional treatment regimens. The improvement of neovascularization and repair processes by administration of bone marrow-derived cells modestly improved the recovery after acute myocardial infarction. However, circulating pat...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.3727/096368912X654957
更新日期:2012-01-01 00:00:00
abstract::Glioblastoma multiforme (GBM) displays high resistance to radiation and chemotherapy, due to the presence of a fraction of GBM stem-like cells (GSLCs), which are thus representing the target for GBM elimination. Since mesenchymal stem cells (MSCs) display high tumor tropism, we examined possible antitumor effects of t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X687787
更新日期:2015-01-01 00:00:00
abstract::Gene therapy as well as methods capable of returning cells to a pluripotent state (iPS) have enabled the correction of genetic deficiencies in syngenic adult progenitors, reducing the need for immunosuppression in cell therapy approaches. However, in diseases involving mutations that lead to the complete lack of a pr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X545068
更新日期:2011-01-01 00:00:00
abstract::In diabetic patients cardiovascular morbidity and mortality is still a major problem. Our aim was to study the effect of kidney-pancreas transplantation on survival, cardiovascular events, and causes of death in diabetic type 1 uremic patients. Three hundred and thirty-three uremic IDDM patients were enrolled in our w...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900621
更新日期:2000-11-01 00:00:00
abstract::End-stage organ failure is a major cause of death worldwide that can occur in patients of all ages and transplantation is the current standard of care for chronic end-stage disease of many organs. Despite the success of organ transplantation, it is becoming clear that there will never be enough organs made available t...
journal_title:Cell transplantation
pub_type: 历史文章,杂志文章
doi:10.3727/000000006783982340
更新日期:2006-01-01 00:00:00
abstract::Liver cell transplantation into host organs like the spleen may possibly provide a temporary relief after extensive liver resection or severe liver disease or may enable treatment of an enzyme deficiency. With time, however, dedifferentiation or malignant transformation of the ectopically transplanted cells may be pos...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000003108746858
更新日期:2003-01-01 00:00:00
abstract::Mechanical thrombectomy (MT) is effective in managing patients with acute ischemic stroke (AIS) caused by large-vessel occlusions and allows for valuable histological analysis of thrombi. However, whether bridging therapy (pretreatment with intravenous thrombolysis before MT) provides additional benefits in patients w...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718823206
更新日期:2019-06-01 00:00:00
abstract::Epithelial wound repair assures the recovery of the epithelial barrier after wounding. During wound healing epithelial cells migrate to cover the wound surface. The presented experiments were carried out to compare the migration of human keratinocytes from primary and secondary culture on polystyrene, collagen, and fi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::Transplantation of human cells after isolation and culture has become an important alternative for treatment of acute or chronic skin wounds. To increase the efficacy and reduce cost for transplantation of skin cells, more efficient and accurate techniques for evaluation of cell proliferation are needed. Hemocytometer...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783982115
更新日期:2006-01-01 00:00:00
abstract::We examined a possible protective effect of the nonpeptidyl low molecular weight radical scavenger IAC [bis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decanedioate di-hydrochloride] on isolated human islet cells against isolation and culture oxidative stress. Islets isolated from pancreases of nondiabetic multiorgan ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908787236639
更新日期:2008-01-01 00:00:00
abstract::Despite the substantial efforts to elucidate the role of early brain injury in subarachnoid hemorrhage (SAH), an effective pharmaceutical therapy for patients with SAH continues to be unavailable. This study aims to reveal the role of necroptosis after SAH, and explore whether the disruption of the blood-brain barrier...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719867285
更新日期:2019-11-01 00:00:00
abstract::The present study was designed to evaluate the use of collagen gel loaded with human retinal pigment epithelium (ARPE19) in cellular transfer and to assess its viability within the gel. Collagen solution was prepared by dissolving calfskin in hydrochloric acid to make a final concentration of 2.0 mg/ml and this was mi...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983666
更新日期:2004-01-01 00:00:00
abstract::Hypoxia is an adverse condition that can jeopardize the function of a bioartificial pancreatic construct. In this study we have investigated the effects of short-term hypoxic exposure (up to 24 h) on the bioenergetic status, metabolism, and insulin secretion of perfused pancreatic constructs composed of alginate/poly-...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900312
更新日期:2000-05-01 00:00:00
abstract::Embedded-culture of pancreatic beta-cells derived from a transgenic mouse insulinoma (MIN6 cells) was studied in vitro and in vivo. The MIN6 cells were enmeshed in an agarose-PVMA-collagen matrix for long-term maintenance. The cells formed islet-like cell clusters (ICCs) in the mixed matrix. When 10 mmol/L nicotinamid...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1016/0963-6897(95)00001-e
更新日期:1995-05-01 00:00:00