Abstract：
:Endothelial progenitor cells (EPCs) have been applied for cell therapy because of their roles in angiogenesis and neovascularization in ischemic tissue. However, adverse responses caused by EPC therapy have not been fully investigated. In this study, a human peripheral blood sample was collected from a healthy donor and peripheral blood mononuclear cells were separated using Ficoll-Hypaque. There were four experimental groups: 10 ml saline infusion group (injection rate; 3 ml/min), 10 ml saline bolus group (injection rate; 60 ml/min), 10 ml EPCs infusion group (2 x 105 cells/ml, injection rate; 3 ml/min), 10 ml EPCs bolus group (2 × 105 cells/ml, injection rate; 60 ml/min). Clinical assessment included physical examination and laboratory examination for intravenous human EPC transplantation in dogs. The results revealed no remarkable findings in vital signs among the dogs used. In blood analysis, platelet counts in saline infusion groups were significantly higher than in the EPC groups within normal ranges, and no significant differences were observed except K+, Cl- and blood urea nitrogen/urea. In ELISA assay, no significant difference was observed in serum tumor necrosis factor alpha. The serum concentration of vascular endothelial growth factor was significantly higher in EPC groups than in saline groups, and interleukin 10 was significantly up-regulated in the EPC infusion group compared with other groups. In conclusion, we demonstrated that no clinical abnormalities were detected after intravenous transplantation of human EPCs in dogs. The transplanted xenogenic EPCs might be involved in anti-inflammatory and angiogenic functions in dogs.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Lee SH,Ra JC,Oh HJ,Kim MJ,Setyawan EMN,Choi YB,Yang JW,Kang SK,Han SH,Kim GA,Lee BCdoi
10.1177/0963689718821686subject
Has Abstractpub_date
2019-07-01 00:00:00pages
943-954issue
7eissn
0963-6897issn
1555-3892journal_volume
28pub_type
杂志文章abstract：:We attempted to regenerate bone in a significant osseous defect with various stem cells from deciduous teeth, extracted from puppies, and grafted them into a parent canine mandible as an allograft, parent dental pulp, and bone marrow by tissue engineering and regenerative medicine technology using platelet-rich plasm...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368910X539128
更新日期：2011-01-01 00:00:00
abstract：:Rare cells are present in human umbilical cord blood that do not express the hematopoietic marker CD45 and in culture do not produce cells of hematopoietic lineage. These umbilical cord multipotent stem cells (UC-MC) behave as multilineage progenitor cells (stem cells) and can be expanded in tissue culture. Exposure t...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096020197390022
更新日期：2002-04-01 00:00:00
abstract：:Multiple sclerosis (MS) is a severe debilitating disorder characterized by progressive demyelination and axonal damage of the central nervous system (CNS). Current therapies for MS inhibit the immune response and demonstrate reasonable benefits if applied during the early phase of relapsing–remitting MS (RRMS) while t...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X657404
更新日期：2013-01-01 00:00:00
abstract：:The requirement for more effective cryopreservation (CP) methodologies in support of the emerging fields of cell bioprocessing and cell therapy is now critical. Current CP strategies appropriately focus on minimizing the damaging actions of physicochemical stressors and membrane disruption associated with extra- and i...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:Currently, different techniques to expand human hepatocytes in vitro are being investigated to generate enough cells for liver-directed cell therapies. However, based on observations in fibroblasts and other cell types, telomere attrition limits the proliferative capacity of normal somatic cells. Therefore, we explore...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000003771000138
更新日期：2003-01-01 00:00:00
abstract：:Spinocerebellar ataxia type 1 (SCA1) is a dominant inherited disease caused by expanded trinucleotide repeats resulting in an increased polyglutamine tract in the gene product. As a potential therapeutic approach for SCA1, we tested antisense RNAs targeting two regions of the ataxin-1 message. Single-stranded regions ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368908786516729
更新日期：2008-01-01 00:00:00
abstract：:Hepatocyte transplantation is an alternative therapy to orthotopic liver transplantation for the treatment of liver diseases. However, the supply of hepatocytes is limited given the shortage of organs available to isolate good-functioning quality cells. Neonatal livers may be a potential source alternative to adult li...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368913X669743
更新日期：2014-01-01 00:00:00
abstract：:Achieving good islet isolation is one of the most important factors for successful islet transplantation. Porcine pancreas is suitable for islet isolation research due to its anatomical and physiological similarities to human pancreas. In this study, we evaluated a new porcine islet isolation method designed to maximi...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000005783982512
更新日期：2005-01-01 00:00:00
abstract：:Cardiovascular disease is a major cause of morbidity, disability, and mortality in kidney transplant patients. Cumulative reports indicate that the excessive risk of cardiovascular events is not entirely explained by the increased prevalence of traditional cardiovascular risk factors. Atherosclerosis is a chronic infl...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.1177/0963689717735404
更新日期：2017-09-01 00:00:00
abstract：:Multiple system atrophy (MSA) is a neurodegenerative disorder that occurs sporadically and causes parkinsonism, cerebellar, autonomic, urinary, and pyramidal dysfunction in many combinations. Progressive L-dopa-unresponsive parkinsonism due to underlying striatonigral degeneration dominates the clinical syndrome in th...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.1177/096368970000900213
更新日期：2000-03-01 00:00:00
abstract：:Articular cartilage is an avascular tissue composed of chondrocytes, a unique cell type responsible for abundant matrix synthesis and maintenance. When damaged, it never heals spontaneously under physiological circumstances. Therefore, the delivery of mesenchymal stem cells using hydrogel has been considered for cart...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368910X557191
更新日期：2011-01-01 00:00:00
abstract：BACKGROUND:Extracellular vesicles (EVs) released from mesenchymal stem/stromal cells (MSCs) mediate their paracrine effect, but their efficacy to protect the microcirculation of the kidney is unknown. Using a novel swine model of unilateral renovascular disease (RVD) complicated by metabolic syndrome (MetS), we tested ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689718780942
更新日期：2018-07-01 00:00:00
abstract：:Autism spectrum disorders (ASDs) are heterogeneous complex neurodevelopmental pathologies defined by behavioral symptoms, but which have well-characterized genetic, immunological, and physiological comorbidities. Despite extensive research efforts, there are presently no agreed upon therapeutic approaches for either t...
journal_title：Cell transplantation
pub_type： 临床试验,杂志文章
doi：10.3727/096368914X684916
更新日期：2014-01-01 00:00:00
abstract：:The technologies for adipose tissue harvesting, processing, and transplantation have substantially evolved in the past two decades. Clinically driven advancements have paralleled a significant improvement in the understanding of cellular, molecular, and immunobiological events surrounding cell and tissue transplantati...
journal_title：Cell transplantation
pub_type： 杂志文章,评审
doi：10.3727/096368910X507187
更新日期：2010-01-01 00:00:00
abstract：:Changes in morphology, metabolism, myosin heavy chain gene expression, and functional performances in damaged rabbit muscles with or without transplantation of primary satellite cells were investigated. For this purpose, we damaged bilaterally the fast muscle tibialis anterior (TA) with either 1.5 or 2.6 ml cardiotoxi...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:The development of new cell replacement strategies using neural stem cells (NSC) may provide an alternative and unlimited cell source for clinical neural transplantation in neurodegenerative diseases such as Parkinson's and Huntington's disease. The clinical application of neural transplantation using NSC will therefo...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000006783464426
更新日期：2006-01-01 00:00:00
abstract：:During the isolation of human islets of Langerhans the digest has repeated direct contact with the ambient atmosphere. In order to fulfill GMP requirements in clinical applications, the entire cell preparation must be performed in clean room facilities. We hypothesized that the use of a closed system, which avoids the...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000007783465091
更新日期：2007-01-01 00:00:00
abstract：:Islet-like cell clusters generated from human fetal pancreases between 18-24 wk gestation were able to grow and mature both morphologically and functionally after transplantation under the kidney capsule of athymic nude mice. Grafted tissue, predominantly ductal in appearance was unresponsive to glucose 2 wk after gra...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/096368979400300509
更新日期：1994-09-01 00:00:00
abstract：:Intrauterine inflammation is a significant cause of injury to the developing fetal brain. Using a preterm fetal sheep model of in utero infection, we asked whether human amnion epithelial cells (hAECs) were able to reduce inflammation-induced fetal brain injury. Surgery was undertaken on pregnant sheep at ∼105 days ge...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368916X693572
更新日期：2017-04-13 00:00:00
abstract：:Hepatocyte transplantation has been proposed as a technique for bridging patients to whole-organ transplantation, for providing metabolic support during liver failure, and for replacing whole-organ transplantation in certain metabolic liver diseases. Assessment of hepatocyte engraftment has been difficult to measure, ...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000004783983945
更新日期：2004-01-01 00:00:00
abstract：:Cells derived from the placenta have become the focus of extensive research concerning their ability to be used for regenerative medicine or cellular therapies. In a previous study, we characterized amnion-derived multipotent progenitor cells, or AMP cells, by in vitro methods and showed they were able to inhibit anti...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368913X670165
更新日期：2014-01-01 00:00:00
abstract：:Membrane integrity fluorescent staining is used routinely to evaluate islet viability. Results are used as one of the determining factors in islet product release criteria, and are used to assess the efficacy of different culture conditions. Recently, it has been observed that there is variation in the viability stain...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/000000004783983701
更新日期：2004-01-01 00:00:00
abstract：:We investigated the effects of environmental enrichment (EE) on the function of transplanted adipose stem cells (ASCs) and the combined effect of EE and ASC transplantation on neurobehavioral function in an animal model of chronic hypoxic-ischemic (HI) brain injury. HI brain damage was induced in 7-day-old mice by uni...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X662390
更新日期：2013-01-01 00:00:00
abstract：:Successful transplantation of cadaver embryonic neural tissue is highly dependent on the method used to obtain the tissue. It is important that the tissue not be contaminated bacteriologically by vaginal flora during the procedure, and that it not be disrupted mechanically. A low-pressure aspiration abortion technique...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/096368979400300202
更新日期：1994-03-01 00:00:00
abstract：:Pancreatic beta cell lines may offer an abundant source of cells for beta-cell replacement in type I diabetes. Using regulatory elements of the bacterial tetracycline (tet) operon for conditional expression of SV40 T antigen oncoprotein in transgenic mouse beta cells, we have shown that reversible immortalization is a...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：
更新日期：2001-01-01 00:00:00
abstract：:This invited concise review was written for the special issue of Cell Transplantation to celebrate the 25th anniversary of the American Society for Neural Therapy and Repair (ASNTR). I aimed to present a succinct summary of two interweaved lines of research work carried out by my team members and collaborators over th...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689719850088
更新日期：2019-04-01 00:00:00
abstract：:Cell therapies consist in transplanting healthy cells into a disabled tissue with the goal to repopulate it and restore its function at least partially. In muscular diseases, most of the time, myoblasts are chosen for their expansion capacity in culture. Nevertheless, cell transplantation has limitations, among them,...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368912X655028
更新日期：2012-01-01 00:00:00
abstract：:In multiple sclerosis (MS), a chronic inflammatory relapsing demyelinating disease, failure to control or repair damage leads to progressive neurological dysfunction and neurodegeneration. Implantation of neural stem cells (NSCs) has been shown to promote repair and functional recovery in the acute experimental autoi...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.3727/096368911X637443
更新日期：2012-01-01 00:00:00
abstract：:Head and neck squamous cell carcinoma (HNSCC) is a malignant tumor of the upper aerodigestive tract affecting the oral cavity, lips, paranasal sinuses, larynx, and nasopharynx. Proteogenomics combines proteomics and genomics and employs mass spectrometry and high-throughput sequencing technologies to identify novel pe...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720929308
更新日期：2020-01-01 00:00:00
abstract：:There is still a lack of sufficient research on the mechanism behind neurogenic bladder (NB) treatment. The aim of this study was to explore the effect of overexpressed stromal cell-derived factor-1 (SDF-1) secreted by engineered immortalized mesenchymal stem cells (imMSCs) on the NB. In this study, primary bone marro...
journal_title：Cell transplantation
pub_type： 杂志文章
doi：10.1177/0963689720902466
更新日期：2020-01-01 00:00:00
