Abstract:
:Intrauterine inflammation is a significant cause of injury to the developing fetal brain. Using a preterm fetal sheep model of in utero infection, we asked whether human amnion epithelial cells (hAECs) were able to reduce inflammation-induced fetal brain injury. Surgery was undertaken on pregnant sheep at ∼105 days gestation (term is 147 days) for implantation of vascular catheters. Lipopolysaccharide (LPS; 150 ng/kg bolus) or saline was administered IV at 109, 110, and 111 days. Sixty million fluorescent-labeled hAECs were administered at 110, 111, and 112 days gestation via the brachial artery catheter. Brains were collected at 114 days for histological assessment. hAECs were observed within the cortex, white matter, and hippocampus. Compared to control lambs, LPS administration was associated with significant and widespread fetal brain inflammation and injury as evidenced by increased number of activated microglia in the periventricular white matter (p = 0.02), increased pyknosis, cell degeneration (p = 0.01), and a nonsignificant trend of fewer oligodendrocytes in the subcortical and periventricular white matter. Administration of hAECs to LPS-treated animals was associated with a significant mitigation in both inflammation and injury as evidenced by fewer activated microglia (p = 0.03) and pyknotic cells (p = 0.03), significantly more oligodendrocytes in the subcortical and periventricular white matter (p = 0.01 and 0.02, respectively), and more myelin basic protein-positive cells within the periventricular white matter (p = 0.02). hAEC administration to fetal sheep exposed to multiple doses of LPS dampens the resultant fetal inflammatory response and mitigates associated brain injury.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Yawno T,Sabaretnam T,Li J,McDonald C,Lim R,Jenkin G,Wallace EM,Miller SLdoi
10.3727/096368916X693572subject
Has Abstractpub_date
2017-04-13 00:00:00pages
541-553issue
4eissn
0963-6897issn
1555-3892journal_volume
26pub_type
杂志文章abstract::At present, there are still significant barriers that impede the clinical use of hESCs and iPS cells, including ethics, immunorejection, tumorigenesis from hESCs, and teratoma formation from iPS cells. It is therefore necessary to search for alternative sources of stem cells. WJ-MSCs originate from embryonic epiblasts...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X662444
更新日期:2013-01-01 00:00:00
abstract::Membrane integrity fluorescent staining is used routinely to evaluate islet viability. Results are used as one of the determining factors in islet product release criteria, and are used to assess the efficacy of different culture conditions. Recently, it has been observed that there is variation in the viability stain...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000004783983701
更新日期:2004-01-01 00:00:00
abstract::Cell-based therapy for the bladder has its beginnings in the 1990s with the successful isolation and culture of bladder smooth muscle cells. Since then, several attempts have been made to artificially implant native cell types and stem cell-derived cells into damaged bladders in the form of single-cell injectables or ...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.3727/096368914X685050
更新日期:2015-01-01 00:00:00
abstract::The main challenge of cell transplantation as a treatment of myopathies is the large amount of tissue to treat. Intravascular delivery of cells may be an ideal route if proven to be effective and safe. Given the importance of nonhuman primates for preclinical research in transplantation, we tested the intra-arterial i...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X685032
更新日期:2014-01-01 00:00:00
abstract::The choice of enzyme blend is critical for successful islet isolation. Islet yield, viability, integrity, and function are important factors that influence the outcome of islet transplantation. Liberase HI has been used as a standard enzyme for pancreas digestion and has successfully produced islets that reversed dia...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X623826
更新日期:2012-01-01 00:00:00
abstract::Cells derived from the placenta have become the focus of extensive research concerning their ability to be used for regenerative medicine or cellular therapies. In a previous study, we characterized amnion-derived multipotent progenitor cells, or AMP cells, by in vitro methods and showed they were able to inhibit anti...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X670165
更新日期:2014-01-01 00:00:00
abstract::Human embryonic stem (hES) cells have the ability to differentiate into a variety of different cell lineages and potentially provide a source of differentiated cells for many therapeutic uses. Here we investigated an efficient method of hepatic differentiation from hES cells. A human ES cell line, KhES-1, was used and...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783981305
更新日期:2006-01-01 00:00:00
abstract::Apoptosis is a vital pathological factor that accounts for the poor prognosis of traumatic spinal cord injury (t-SCI). The 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB3) is a critical regulator for energy metabolism and proven to have antiapoptotic effects. This study aimed to investigate the neuroprote...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720950226
更新日期:2020-01-01 00:00:00
abstract::Cell therapies consist in transplanting healthy cells into a disabled tissue with the goal to repopulate it and restore its function at least partially. In muscular diseases, most of the time, myoblasts are chosen for their expansion capacity in culture. Nevertheless, cell transplantation has limitations, among them,...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X655028
更新日期:2012-01-01 00:00:00
abstract::In previous work, we engineered functional cell sheets using bone marrow-derived mesenchymal stem cells (BM-MSCs) to promote islet graft survival. In the present study, we hypothesized that a cell sheet using dermal fibroblasts could be an alternative to MSCs, and then we aimed to evaluate the effects of this cell she...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X690854
更新日期:2016-01-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a fatal disease of motor neuron degeneration in the brain and spinal cord. Progressive paralysis of the diaphragm and other respiratory muscles leading to respiratory dysfunction and failure is the most common cause of death in ALS patients. Respiratory impairment has also been s...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720913494
更新日期:2020-01-01 00:00:00
abstract::Several clinical trials of bone marrow cell infusion in patients with liver cirrhosis (LC) have shown clinical improvement, despite conflicting results from animal models. We investigated serial pathological features and the clinical impact after autologous bone marrow infusion (ABMI) in patients with advanced LC. Ten...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X506863
更新日期:2010-01-01 00:00:00
abstract::Insufficient oxygenation can limit the long-term survival of encapsulated islets in subcutaneous tissue. Transplantation of coencapsulated pig islets with adipose or bone marrow mesenchymal stem cells (AMSCs or BM-MSCs, respectively) was investigated with regard to implant vascularization, oxygenation, and diabetes co...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368913X663550
更新日期:2014-01-01 00:00:00
abstract::The objective of this study was to define pretransplant islet culture conditions for optimum tissue engraftment in the rat islet allotransplantation model. Lewis rat islets were cultured in TCM 199/5% fetal calf serum for I day at 37 degrees C, followed by 1 day of culture at 22 degrees C. When islets from single dono...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::Cerebral palsy (CP) is a common disability which results in permanent chronic motor disability appearing in early childhood. Recently human umbilical cord blood mesenchymal stem cell (hUCB-MSC) infusion has emerged as a promising therapeutic strategy for CP, and the treatment efficacy remains to be confirmed by clinic...
journal_title:Cell transplantation
pub_type: 杂志文章,随机对照试验
doi:10.1177/0963689717729379
更新日期:2018-02-01 00:00:00
abstract::Pluripotent stem cells, including human embryonic stem cells and induced pluripotent stem cells, have generated much excitement about their prospects for use in cell transplantation therapies. This is largely attributable to their virtually unlimited growth potential, their ability to be precisely genetically altered ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X498944
更新日期:2010-01-01 00:00:00
abstract::Critical length nerve defects in the rat sciatic nerve model were reconstructed with chitosan nerve guides filled with Schwann cells (SCs) containing hydrogel. The transplanted SCs were naive or had been genetically modified to overexpress neurotrophic factors, thus providing a cellular neurotrophic factor delivery sy...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X688010
更新日期:2016-01-01 00:00:00
abstract::Gene therapy as well as methods capable of returning cells to a pluripotent state (iPS) have enabled the correction of genetic deficiencies in syngenic adult progenitors, reducing the need for immunosuppression in cell therapy approaches. However, in diseases involving mutations that lead to the complete lack of a pr...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X545068
更新日期:2011-01-01 00:00:00
abstract::Cord blood is an attractive cell source in regenerative medicine and represents an alternative to bone marrow. The aim of this study was to investigate whether human umbilical cord blood mononuclear (HUCBM) cells might be valuable in hepatic regenerative medicine. HUCBM cells differentiated in vitro into hepatocytes, ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908786516837
更新日期:2008-01-01 00:00:00
abstract::Large-scale sterile methods for isolating hepatocytes are desirable for the development of bioartificial liver support systems. In this study the traditional centrifuge method was compared with the use of a Baylor Rapid Autologous Transfusion (BRAT) machine for isolating large quantities of porcine hepatocytes. After ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000005783983007
更新日期:2005-01-01 00:00:00
abstract::We attempted to regenerate bone in a significant osseous defect with various stem cells from deciduous teeth, extracted from puppies, and grafted them into a parent canine mandible as an allograft, parent dental pulp, and bone marrow by tissue engineering and regenerative medicine technology using platelet-rich plasm...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368910X539128
更新日期:2011-01-01 00:00:00
abstract::Gastric cancer (GC) is a leading cause of cancer-related death with poor prognosis. Growing evidence has shown that long noncoding ribonucleic acid (lncRNA) FEZ family zinc finger 1 antisense RNA 1(FEZF1-AS1), an "oncogene," regulates tumor progression and supports cancer stem cell. However, the tumorigenic mechanism ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720925059
更新日期:2020-01-01 00:00:00
abstract::Mononuclear stem cells have been studied for their potential in myocardial ischemia. In our previous published article, ReACT(®) phase I/II clinical trial, our results suggest that a certain cell population, promonocytes, directly correlated with the perceived angiogenesis in refractory angina patients. This study is ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X681595
更新日期:2015-01-01 00:00:00
abstract::Rare cells are present in human umbilical cord blood that do not express the hematopoietic marker CD45 and in culture do not produce cells of hematopoietic lineage. These umbilical cord multipotent stem cells (UC-MC) behave as multilineage progenitor cells (stem cells) and can be expanded in tissue culture. Exposure t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096020197390022
更新日期:2002-04-01 00:00:00
abstract::It is currently said that CO has anti-inflammatory and antiapoptosis effects and it has attracted attention as a medical gas. We used CO for rat hearts and conducted a preservation experiment. We isolated rat hearts, placed them into a specially made chamber, filled the chamber with a gas mixture of PCO (4,000 hPa) a...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X605547
更新日期:2012-01-01 00:00:00
abstract::To engineer liver tissues with a clinically significant size, in vivo evaluation of performance using large-scale animal studies are necessary before proceeding to human clinical trials. As pigs are the most suitable candidates, the development of culture conditions suitable for porcine hepatocyte progenitors is very ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000006783981486
更新日期:2006-01-01 00:00:00
abstract::Pigs share many anatomical and physiological features with humans, offering a unique and viable model for biomedical research. Although porcine female germline stem cells (FGSCs) were identified in the juvenile ovary, no reports described the isolation and purification of FGSCs from the pig at sexual maturity. Here, w...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718784878
更新日期:2018-08-01 00:00:00
abstract::Despite recent advances and promising results in children, liver cell transplantation (LCT) should still be regarded as an experimental therapy. Several substantial complications are known from animal studies and individual patients. However, safety data on liver cell infusion in children are scarce. We used LCT in fo...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X485058
更新日期:2010-01-01 00:00:00
abstract::Testicular germ cell tumors (TGCTs) are highly prevalent in young men aged 20-40 years and are one of the most common lethal solid tumors in men of this age. Due to the current unclear mechanism of tumor development, there is a lack of effective treatment, and therefore in-depth research of the molecular mechanism of ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689720946653
更新日期:2020-01-01 00:00:00
abstract::Pancreatic islet transplantation has the potential to be an effective treatment for type 1 diabetes mellitus. While recent improvements have improved 1-year outcomes, follow-up studies show a persistent loss of graft function/survival over 5 years. One possible cause of islet transplant failure is the immunosuppressan...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X471198
更新日期:2009-01-01 00:00:00