Abstract:
:Testicular germ cell tumors (TGCTs) are highly prevalent in young men aged 20-40 years and are one of the most common lethal solid tumors in men of this age. Due to the current unclear mechanism of tumor development, there is a lack of effective treatment, and therefore in-depth research of the molecular mechanism of the occurrence and development of TGCT and the search for suitable and effective therapeutic targets and molecular markers are of great significance for achieving effective treatment. METTL3 is a very important methylase, which has been implicated in the progression of many cancers, but the role of METTL3 in TGCT has not been fully elucidated. In this article, we found that METTL3 expression was significantly downregulated in TGCT tissues, and patients with low expression levels had lower overall survival and relapse-free survival rates. After overexpressing METTL3, cell proliferation, invasion, and migration ability significantly increased, while influencing the expression of epithelial-mesenchymal transition (EMT)-related proteins. In addition, we observed that the expression level of METTL3 positively correlated with molecular markers and infiltration level of CD8+ and CD4+ T cells and natural killer cells. In sum, our findings identified that METTL3 can be used as an independent prognostic marker in patients with TGCT. METTL3 participates in the proliferation, migration, and invasion of TGCT cells by regulating the expression of EMT-related genes and may also play a role in activating the tumor immune response in TGCT.
journal_name
Cell Transplantjournal_title
Cell transplantationauthors
Luo Y,Sun Y,Li L,Mao Ydoi
10.1177/0963689720946653subject
Has Abstractpub_date
2020-01-01 00:00:00pages
963689720946653eissn
0963-6897issn
1555-3892journal_volume
29pub_type
杂志文章abstract::Techniques for medical tissue regeneration require an abundant source of human adult stem cells. There is increasing evidence that adipose stem cells contribute to restoration of tissue vascularization and organ function. The object of our study was to isolate and characterize adult adipose-derived stem cells from pat...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/09638910X519265
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abstract::The clinical consequences of acute liver failure are associated with high mortality. Intensive medical intervention is required to treat the symptoms of liver failure, including coagulopathy, metabolic instability, and encephalopathy. Providing temporary liver support with an extracorporeal liver assist device could s...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900101
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abstract::The limited availability of human embryonic tissue for dopamine cell transplants in Parkinson's patients has led to an increased interest in using xenogeneic donor tissue. Unfortunately, without aggressive immunosuppression, such brain xenografts are rejected by the host immune system. Chronic brain xenograft rejectio...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
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abstract::Angiopoietin-1 (Ang1) mediates vascular maturation and immune response. Diabetes decreases Ang1 expression and disrupts Ang1/Tie2 signaling activity. Vasculotide is an Ang1 mimetic peptide, and has anti-inflammatory effects. In this study, we test the hypothesis that vasculotide treatment induces neuroprotection and d...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718791568
更新日期:2018-12-01 00:00:00
abstract::Stroke is the third leading cause of death worldwide and a huge perpetrator in adult disability. This pilot clinical study investigates the possible benefits of transplanting multiple cells in chronic stroke. A total of 10 consecutive stroke patients were treated by combination cell transplantation on the basis of an ...
journal_title:Cell transplantation
pub_type: 临床试验,杂志文章
doi:10.3727/096368913X672154
更新日期:2013-01-01 00:00:00
abstract::Ischemic stroke remains a serious threat to human life. There are limited effective therapies for the treatment of stroke. We have previously demonstrated that angiogenesis and neurogenesis in the brain play an important role in functional recovery following ischemic stroke. Recent studies indicate that increased arte...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718771889
更新日期:2018-04-01 00:00:00
abstract::Neuronal progenitor cells (NPCs) derived from human embryonic stem cells (hESCs) are an excellent cell source for transplantation therapy due to their availability and ethical acceptability. However, the traditional method of expansion and differentiation of hESCs into NPCs in monolayer cultures requires a long time, ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368915X690378
更新日期:2016-01-01 00:00:00
abstract::Adipose tissue contains a mesenchymal stem cell (MSC) population known as adipose-derived stem cells (ASCs) capable of differentiating into different cell types. Our aim was to induce hepatic transdifferentiation of ASCs by sequential exposure to several combinations of cytokines, growth factors, and hormones. The mos...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X12483162197321
更新日期:2009-01-01 00:00:00
abstract::Pancreatic islet transplantation has the potential to be an effective treatment for type 1 diabetes mellitus. While recent improvements have improved 1-year outcomes, follow-up studies show a persistent loss of graft function/survival over 5 years. One possible cause of islet transplant failure is the immunosuppressan...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X471198
更新日期:2009-01-01 00:00:00
abstract::Near infrared fluorescence (NIRF) optical imaging is a technique particularly powerful when studying in vivo processes at the molecular level in preclinical animal models. We recently demonstrated liver irradiation under the additional stimulus of partial hepatectomy as being an effective primer in the rat liver repop...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::Bone marrow-derived cells have different plastic properties, especially regarding cell fusion, which increases with time and is prompted by tissue injury. Several recessive mutations, including Purkinje Cell Degeneration, affect the number of Purkinje cells in homozygosis; heterozygous young animals have an apparently...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X638900
更新日期:2012-01-01 00:00:00
abstract::Cell therapy has attracted considerable interest as a promising therapeutic alternative for patients with Parkinson's disease (PD). Clinical studies have shown that grafted fetal neural tissue can achieve considerable biochemical and clinical improvements in PD. However, the source of fetal tissue grafts is limited an...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368916X691682
更新日期:2016-11-01 00:00:00
abstract::Multilineage-differentiating stress-enduring (Muse) cells are endogenous pluripotent stem cells that can be isolated based on stage-specific embryonic antigen-3 (SSEA-3), a pluripotent stem cell-surface marker. However, their capacities for survival, neurotrophic factor secretion, and neuronal and glial differentiatio...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719863809
更新日期:2019-09-01 00:00:00
abstract::Acute myocardial infarction (AMI) results in ischemic damage and death of cardiomyocytes and loss of vasculature. Stem cell therapy has emerged as a potentially promising strategy for maximizing cardiac function following ischemic injury. Issues of cell source, delivery, and quantification of response have challenged...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X603657
更新日期:2012-01-01 00:00:00
abstract::Efficient maintenance of the undifferentiated status of embryonic stem cells (ESCs) may be important for preparation of high-quality cell sources that can be successfully used for stem cell research and therapy. Here we tried to identify a compound that can enhance the quality of pluripotent stem cells. Treatment of E...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368914X683511
更新日期:2015-01-01 00:00:00
abstract::As an alternative to drug immunosuppression, attempts at inducing donor-specific tolerance by intrathymic (IT) inoculations to transplant recipient of donor origin alloantigenic products has proven very promising. Using fiber optic thoracoscopy, a technique for the study of this phenomena was developed for the dog. We...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368979400300413
更新日期:1994-07-01 00:00:00
abstract::Cell therapy is a promising treatment modality to improve heart function in acute myocardial infarction. However, the mechanisms of action and the most suitable cell type have not been finally determined. We performed a study to compare the effects of mesenchymal stem cells (MSCs) harvested from different tissues on ...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368911X627462
更新日期:2012-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689718784878
更新日期:2018-08-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970901805-614
更新日期:2009-01-01 00:00:00
abstract::Bone nonunion is a pathological condition in which all bone healing processes have stopped, resulting in abnormal mobility between 2 bone segments. The incidence of bone-related injuries will increase in an aging population, leading to such injuries reaching epidemic proportions. Tissue engineering and cell therapy us...
journal_title:Cell transplantation
pub_type: 杂志文章,评审
doi:10.1177/0963689717721203
更新日期:2017-09-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368912X658728
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/096368970000900621
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journal_title:Cell transplantation
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doi:10.1177/0963689720978219
更新日期:2021-01-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.1177/0963689719864318
更新日期:2019-11-01 00:00:00
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368909X12483162196647
更新日期:2009-01-01 00:00:00
abstract::This study is a preliminary investigation to determine if bone marrow-derived cells, when implanted into freeze-injured urinary bladders, differentiate into smooth muscle cells and reconstruct smooth muscle layers. Bone marrow cells were harvested from femurs of male ICR mice and cultured in collagen-coated dishes for...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096368908784153850
更新日期:2008-01-01 00:00:00
abstract::Rare cells are present in human umbilical cord blood that do not express the hematopoietic marker CD45 and in culture do not produce cells of hematopoietic lineage. These umbilical cord multipotent stem cells (UC-MC) behave as multilineage progenitor cells (stem cells) and can be expanded in tissue culture. Exposure t...
journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/096020197390022
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journal_title:Cell transplantation
pub_type: 杂志文章
doi:10.3727/000000001783986756
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abstract::Liver transplantation is the only existing modality for treating decompensated liver cirrhosis. Several factors, such as nonavailability of donors, combined with operative risks, complications associated with rejection, usage of immunosuppressive agents, and cost intensiveness, make this strategy available to only a f...
journal_title:Cell transplantation
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pub_type: 杂志文章,随机对照试验
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