Low-pressure aspiration abortion for obtaining embryonic and early gestational fetal tissue for research purposes.

Abstract:

:Successful transplantation of cadaver embryonic neural tissue is highly dependent on the method used to obtain the tissue. It is important that the tissue not be contaminated bacteriologically by vaginal flora during the procedure, and that it not be disrupted mechanically. A low-pressure aspiration abortion technique has been developed that allows for the safe, effective obtainment of embryonic tissue and reduces the risk of transmitting infection. Tissue was cultured in vitro in 102 cases with minimal evidence of contamination by vaginal flora. Transplants of neural tissue into over 300 rodents have resulted in no intracranial abscesses, even in the setting of immunosuppression. The suction apparatus and low-pressure aspiration minimize disruption of the embryonic tissue. Use of low pressure adds no significant additional risk to the patient. In over 300 cases, there have been no medical complications specifically attributable to the technique. Because local anesthetic is used and sonography is not routinely required, the procedure can easily be performed in an outpatient setting during routine elective abortions, with minimal slowing or disruption of the clinic's surgical schedule. In conclusion, the low-pressure aspiration abortion technique can be safely and effectively used to obtain embryonic and early gestational fetal tissue that is almost always free from bacterial, fungal, and yeast contamination and that is frequently structurally intact. It requires no significant alteration in indications for abortion, risks, methodology, timing of the abortion, or patient management.

journal_name

Cell Transplant

journal_title

Cell transplantation

authors

Nauert GM,Freeman TB

doi

10.1177/096368979400300202

subject

Has Abstract

pub_date

1994-03-01 00:00:00

pages

147-51

issue

2

eissn

0963-6897

issn

1555-3892

journal_volume

3

pub_type

杂志文章
  • ET-Kyoto solution plus dibutyryl cyclic adenosine monophosphate is superior to University of Wisconsin solution in rat liver preservation.

    abstract::ET-Kyoto solution (ET-K) is an extracellular-type organ preservation solution containing the cytoprotective disaccharide, trehalose. A previous study reported the supplement of dibutyryl cyclic adenosine monophosphate (db-cAMP) in conventional ET-K to attenuate lung ischemia-reperfusion injury. In this study, the effi...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/000000008783906928

    authors: Zhao X,Koshiba T,Nakamura T,Tsuruyama T,Li Y,Bando T,Wada H,Tanaka K

    更新日期:2008-01-01 00:00:00

  • Isolated pancreatic islets in three-dimensional matrices are responsive to stimulators and inhibitors of angiogenesis.

    abstract::The formation of a new microvasculature is essential for the long-term survival and function of the islet graft. In this study we examined endothelium of isolated pancreatic islets by stimulation with growth factors, different culture conditions, and genetic modification. We also inspected the effect of immunosuppress...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/000000006783981774

    authors: Kidszun A,Schneider D,Erb D,Hertl G,Schmidt V,Eckhard M,Preissner KT,Breier G,Bretzel RG,Linn T

    更新日期:2006-01-01 00:00:00

  • Standard recommendations for the application of Chinese clinical cell therapy for neurorestoration (2012).

    abstract::Cell therapy has been shown to be a promising alternative therapy for neurorestoration, and more than 30 different types of cells have been shown to possess some capability to restore lost or damaged neurological functions. Chinese physicians have successfully applied cell therapy to many neurological diseases and dam...

    journal_title:Cell transplantation

    pub_type: 杂志文章,评审

    doi:10.3727/096368913X672082

    authors: Chinese Branch of International Association of Neurorestoratology (IANR).,Preparatory Committee of Chinese Association of Neurorestoratology.

    更新日期:2013-01-01 00:00:00

  • Neural progenitor cells derived from the adult rat subventricular zone: characterization and transplantation.

    abstract::In order to fully characterize and determine the therapeutic potential of adult neural progenitor cells (NPCs), it is important to be able to isolate and study NPCs from animals such as rats, in which there are existing models of brain injury and disease. The focus of this study was to characterize the cultivation, di...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/000000007783465253

    authors: Chen K,Hughes SM,Connor B

    更新日期:2007-01-01 00:00:00

  • Xenotransplantation of human umbilical cord blood mononuclear cells to rats with D-galactosamine-induced hepatitis.

    abstract::Cord blood is an attractive cell source in regenerative medicine and represents an alternative to bone marrow. The aim of this study was to investigate whether human umbilical cord blood mononuclear (HUCBM) cells might be valuable in hepatic regenerative medicine. HUCBM cells differentiated in vitro into hepatocytes, ...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/096368908786516837

    authors: Alvarez-Mercado AI,Sáez-Lara MJ,García-Mediavilla MV,Sánchez-Campos S,Abadía F,Cabello-Donayre M,Gil A,González-Gallego J,Fontana L

    更新日期:2008-01-01 00:00:00

  • HIV Non-Nucleoside Reverse Transcriptase Inhibitor Efavirenz Reduces Neural Stem Cell Proliferation in Vitro and in Vivo.

    abstract::The prevalence of HIV-associated neurocognitive disorders (HAND) remains high despite combination antiretroviral therapy (cART). There is evidence that neural stem cells (NSCs) can migrate to sites of brain injury such as those caused by inflammation and oxidative stress, which are pathological features of HAND. Thus,...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/096368916X691457

    authors: Jin J,Grimmig B,Izzo J,Brown LAM,Hudson C,Smith AJ,Tan J,Bickford PC,Giunta B

    更新日期:2016-11-01 00:00:00

  • Islet cryopreservation: improved recovery following taurine pretreatment.

    abstract::Simple and efficient freezing methods with maximal postthawing recovery form the basis of ideal cryopreservation. Taurine (2-amino ethanesulfonic acid), an end-product of sulphur amino acid metabolism, is one of the most abundant free amino acids in the body. The membrane stabilizing, free radical scavenging, and osmo...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/000000001783986756

    authors: Hardikar AA,Risbud MV,Remacle C,Reusens B,Hoet JJ,Bhonde RR

    更新日期:2001-01-01 00:00:00

  • An ectopic stromal implant model for hematopoietic reconstitution and in vivo evaluation of bone marrow niches.

    abstract::In adults, hematopoiesis takes places in the bone marrow, where specialized niches containing mesenchymal nonhematopoietic cells (stroma) harbor the hematopoietic stem cell (HSC). These niches are responsible and essential for the maintenance of HSCs. Attempts to expand HSCs fail to keep the general properties of ste...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/096368912X636993

    authors: Paraguassú-Braga FH,Alves AP,Santos IM,Bonamino M,Bonomo A

    更新日期:2012-01-01 00:00:00

  • Expansion of Hepatic and Hematopoietic Stem Cells Utilizing Mouse Embryonic Liver Explants.

    abstract::Ex vivo embryonic liver explant culture is a novel and attractive approach to obtain abundant hepatic and hematopoietic stem cells. Gene therapy of autologous hepatic and hematopoietic stem cells represents an alternative therapeutic approach to liver transplantation for genetic and metabolic disorders. In this study ...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/000000001783986945

    authors: Monga SPS,Tang Y,Candotti F,Rashid A,Wildner O,Mishra B,Iqbal S,Mishra L

    更新日期:2001-01-01 00:00:00

  • In vitro characterization of porcine hepatocyte function.

    abstract::The clinical consequences of acute liver failure are associated with high mortality. Intensive medical intervention is required to treat the symptoms of liver failure, including coagulopathy, metabolic instability, and encephalopathy. Providing temporary liver support with an extracorporeal liver assist device could s...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.1177/096368970000900101

    authors: Gregory PG,Connolly CK,Toner M,Sullivan SJ

    更新日期:2000-01-01 00:00:00

  • Protein transduction: a novel approach to induce in vitro pancreatic differentiation.

    abstract::It is widely believed that human embryonic stem (huES) cells may represent a valid alternative to donor pancreata as a source of islets for transplantation. Much is known about the transcription factors whose sequential activation results in the generation of islets during pancreatic development. This knowledge has be...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/000000006783982359

    authors: Dominguez-Bendala J,Pastori RL,Ricordi C,Inverardi L

    更新日期:2006-01-01 00:00:00

  • Application of human persistent fetal vasculature neural progenitors for transplantation in the inner retina.

    abstract::Persistent fetal vasculature (PFV) is a potentially serious developmental anomaly in human eyes, which results from a failure of the primary vitreous and the hyaloid vascular systems to regress during development. Recent findings from our laboratory indicate that fibrovascular membranes harvested from subjects with P...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/096368912X647153

    authors: Chen G,Ma J,Shatos MA,Chen H,Cyr D,Lashkari K

    更新日期:2012-01-01 00:00:00

  • Neural stem/progenitor cell transplantation for cortical visual impairment in neonatal brain injured patients.

    abstract::The purpose of this study was to investigate the clinical efficacy of neural stem/progenitor cell (NS/PC) transplantation to treat severe cortical visual impairment (CVI), a sequela of neonatal brain injury. Fifty-two patients with cerebral injury and CVI were randomly divided into two groups: the treatment group (n =...

    journal_title:Cell transplantation

    pub_type: 杂志文章,随机对照试验

    doi:10.3727/096368913X672163

    authors: Luan Z,Qu S,Du K,Liu W,Yang Y,Wang Z,Cui Y,Du Q

    更新日期:2013-01-01 00:00:00

  • Iron Dyshomeostasis Induces Binding of APP to BACE1 for Amyloid Pathology, and Impairs APP/Fpn1 Complex in Microglia: Implication in Pathogenesis of Cerebral Microbleeds.

    abstract::As a putative marker of cerebral small vessel disease, cerebral microbleeds (CMBs) have been associated with vascular cognitive impairment. Both iron accumulation and amyloid protein precursor (APP) dysregulation are recognized as pathological hallmarks underlying the progression of CMBs, but their cross-talk is not y...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.1177/0963689719831707

    authors: Gong L,Tian X,Zhou J,Dong Q,Tan Y,Lu Y,Wu J,Zhao Y,Liu X

    更新日期:2019-08-01 00:00:00

  • Morphology and function of cultured human fetal pancreatic cells transplanted into athymic mice: a longitudinal study.

    abstract::Islet-like cell clusters generated from human fetal pancreases between 18-24 wk gestation were able to grow and mature both morphologically and functionally after transplantation under the kidney capsule of athymic nude mice. Grafted tissue, predominantly ductal in appearance was unresponsive to glucose 2 wk after gra...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.1177/096368979400300509

    authors: Beattie GM,Butler C,Hayek A

    更新日期:1994-09-01 00:00:00

  • ALLogeneic Heart STem Cells to Achieve Myocardial Regeneration (ALLSTAR) Trial: Rationale and Design.

    abstract::Autologous cardiosphere-derived cells (CDCs) were the first therapeutic modality to demonstrate myocardial regeneration with a decrease in scar size and an increase in viable, functional tissue. Widespread applicability of autologous CDC therapy is limited by the need for patient-specific myocardial biopsy, cell proce...

    journal_title:Cell transplantation

    pub_type: 杂志文章,多中心研究,随机对照试验

    doi:10.3727/096368916X692933

    authors: Chakravarty T,Makkar RR,Ascheim DD,Traverse JH,Schatz R,DeMaria A,Francis GS,Povsic TJ,Smith RR,Lima JA,Pogoda JM,Marbán L,Henry TD

    更新日期:2017-02-16 00:00:00

  • Mechanical stress-induced orientation and ultrastructural change of smooth muscle cells cultured in three-dimensional collagen lattices.

    abstract::The effect of tensile stress on the orientation and phenotype of arterial smooth muscle cells (SMCs) cultured in three-dimensional (3D) type I collagen gels was morphologically investigated. Ring-shaped hybrid tissues were prepared by thermal gelation of a cold mixed solution of type I collagen and SMCs derived from b...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.1177/096368979400300605

    authors: Kanda K,Matsuda T

    更新日期:1994-11-01 00:00:00

  • Islet isolation from juvenile porcine pancreas after 24-h hypothermic machine perfusion preservation.

    abstract::Pancreas procurement for islet isolation and transplantation is limited by concerns for the detrimental effects of postmortem ischemia. Hypothermic machine perfusion (HMP) preservation technology has had a major impact in circumventing ischemic injury in clinical kidney transplantation and is applied here to the prese...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/096368910X486316

    authors: Taylor MJ,Baicu S,Greene E,Vazquez A,Brassil J

    更新日期:2010-01-01 00:00:00

  • Impact of Age on Human Adipose Stem Cells for Bone Tissue Engineering.

    abstract::Bone nonunion is a pathological condition in which all bone healing processes have stopped, resulting in abnormal mobility between 2 bone segments. The incidence of bone-related injuries will increase in an aging population, leading to such injuries reaching epidemic proportions. Tissue engineering and cell therapy us...

    journal_title:Cell transplantation

    pub_type: 杂志文章,评审

    doi:10.1177/0963689717721203

    authors: Dufrane D

    更新日期:2017-09-01 00:00:00

  • Intrastriatal cografts of autologous adrenal medulla and sural nerve in MPTP-induced parkinsonian macaques: behavioral and anatomical assessment.

    abstract::To examine the effects of autologous sural nerve and adrenal medullary tissue intrastriatal cografts upon voluntary motor performance in parkinsonism, a non-human primate 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model was employed to quantitatively assess skilled hand movements. Motor performance was studie...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.1016/0963-6897(95)92155-r

    authors: Watts RL,Mandir AS,Bakay RA

    更新日期:1995-01-01 00:00:00

  • Dose-Dependent Effects of Zoledronic Acid on Human Periodontal Ligament Stem Cells: An In Vitro Pilot Study.

    abstract::Bisphosphonates (BPs) are widely used to treat several metabolic and oncological diseases affecting the skeletal system. Despite BPs' well-known therapeutic potential, they also displayed important side effects, among which is BPs-related osteonecrosis of the jaw, by targeting osteoclast activities, osteoblast, and os...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.1177/0963689720948497

    authors: Di Vito A,Chiarella E,Baudi F,Scardamaglia P,Antonelli A,Giudice D,Barni T,Fortunato L,Giudice A

    更新日期:2020-01-01 00:00:00

  • Transcriptome Analysis of Induced Pluripotent Stem Cell (iPSC)-derived Pancreatic β-like Cell Differentiation.

    abstract::Diabetes affects millions of people worldwide, and β-cell replacement is one of the promising new strategies for treatment. Induced pluripotent stem cells (iPSCs) can differentiate into any cell type, including pancreatic β cells, providing a potential treatment for diabetes. However, the molecular mechanisms underlyi...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.1177/0963689717720281

    authors: Huang Y,Wan J,Guo Y,Zhu S,Wang Y,Wang L,Guo Q,Lu Y,Wang Z

    更新日期:2017-08-01 00:00:00

  • Effective islet isolation method with extremely high islet yields from adult pigs.

    abstract::Achieving good islet isolation is one of the most important factors for successful islet transplantation. Porcine pancreas is suitable for islet isolation research due to its anatomical and physiological similarities to human pancreas. In this study, we evaluated a new porcine islet isolation method designed to maximi...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/000000005783982512

    authors: Yonekawa Y,Matsumoto S,Okitsu T,Arata T,Iwanaga Y,Noguchi H,Nagata H,O'Neil JJ,Tanaka K

    更新日期:2005-01-01 00:00:00

  • Successful single donor islet allotransplantation in the streptozotocin diabetes rat model.

    abstract::The objective of this study was to define pretransplant islet culture conditions for optimum tissue engraftment in the rat islet allotransplantation model. Lewis rat islets were cultured in TCM 199/5% fetal calf serum for I day at 37 degrees C, followed by 1 day of culture at 22 degrees C. When islets from single dono...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:

    authors: Jahr H,Hussmann B,Eckhardt T,Bretzel RG

    更新日期:2002-01-01 00:00:00

  • The TheraCyte™ device protects against islet allograft rejection in immunized hosts.

    abstract::Clinically, many candidates for islet transplantation are already immunized, which increases their risk of graft rejection. Encapsulation of pancreatic islets using the TheraCyte™ device has been shown to protect against allograft rejection in nonimmunized recipients. However, the capacity of the TheraCyte™ device to ...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/096368912X657486

    authors: Kumagai-Braesch M,Jacobson S,Mori H,Jia X,Takahashi T,Wernerson A,Flodström-Tullberg M,Tibell A

    更新日期:2013-01-01 00:00:00

  • Survival and integration of neurons derived from human embryonic stem cells in MPTP-lesioned primates.

    abstract::A human embryonic stem cell (HESC) line, H1, was studied after differentiation to a dopaminergic phenotype in vitro in order to carry out in vivo studies in Parkinsonian monkeys. To identify morphological characteristics of transplanted donor cells, HESCs were transfected with a GFP lentiviral vector. Gene expression ...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/096368913X664865

    authors: Wakeman DR,Weiss S,Sladek JR,Elsworth JD,Bauereis B,Leranth C,Hurley PJ,Roth RH,Redmond DE

    更新日期:2014-01-01 00:00:00

  • Posttranscriptional mechanisms of glucocorticoid antiproliferative effects: glucocorticoids inhibit IL-6-induced proliferation of B9 hybridoma cells.

    abstract::Addition of rIL-6 to IL-6-dependent B9 cells starved for IL-6 for 16-20 h stimulated a vigorous proliferative response. Glucocorticoids (GCs), in a concentration-dependent manner, inhibited rIL-6-stimulated proliferation of B9 cells This inhibition was specific for the GCs, evident by the capacity of the GCs, dexameth...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:

    authors: Almawi WY,Tamim H

    更新日期:2001-03-01 00:00:00

  • Embedded-culture of pancreatic beta-cells derived from transgenic mouse insulinoma as a potential source for xenotransplantation using a diffusion chamber.

    abstract::Embedded-culture of pancreatic beta-cells derived from a transgenic mouse insulinoma (MIN6 cells) was studied in vitro and in vivo. The MIN6 cells were enmeshed in an agarose-PVMA-collagen matrix for long-term maintenance. The cells formed islet-like cell clusters (ICCs) in the mixed matrix. When 10 mmol/L nicotinamid...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.1016/0963-6897(95)00001-e

    authors: Ohgawara H,Miyazaki J,Karibe S,Tashiro F,Akaike T,Hashimoto Y

    更新日期:1995-05-01 00:00:00

  • Quantitative assay for quality assurance of human cells for clinical transplantation.

    abstract::Transplantation of human cells after isolation and culture has become an important alternative for treatment of acute or chronic skin wounds. To increase the efficacy and reduce cost for transplantation of skin cells, more efficient and accurate techniques for evaluation of cell proliferation are needed. Hemocytometer...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.3727/000000006783982115

    authors: Boyce ST,Anderson BA,Rodriguez-Rilo HL

    更新日期:2006-01-01 00:00:00

  • Follow-up study of the revascularization process of purified rat islet beta-cell grafts.

    abstract::The revascularization of islets of Langerhans transplanted in heterotopic sites like the liver by portal vein embolization or the renal subcapsular space is a major process necessary for the viability of grafted cells. This process has been extensively studied by different techniques and the results have shown that is...

    journal_title:Cell transplantation

    pub_type: 杂志文章

    doi:10.1016/s0963-6897(97)00099-7

    authors: Mendola JF,Conget I,Manzanares JM,Corominola H,Viñas O,Barcelo J,Gomis R

    更新日期:1997-11-01 00:00:00